Click here to switch to the map view.

The map label for this gene is stp [H]

Identifier: 77459499

GI number: 77459499

Start: 3759585

End: 3761126

Strand: Reverse

Name: stp [H]

Synonym: Pfl01_3277

Alternate gene names: 77459499

Gene position: 3761126-3759585 (Counterclockwise)

Preceding gene: 77459500

Following gene: 77459497

Centisome position: 58.42

GC content: 63.23

Gene sequence:

>1542_bases
ATGAAATCCATGACAACCGTCTCCCAATACCAGGGCGCGCGCGAAAAGCTGCTGATCCTCGCGGCCATTTGCCTGTCGGC
GCTGGTCCTTCCGTTAAGTTTTACCGGTGGCGCGGTGGCAACGCCGGCCATCGGCCGTGACCTGGGCGGCAGCCCGGTGG
CGCTGACCTGGATCACCAATGCGTTCATGTTGTCGTTCGGCAGTTTGCTGATGGCGGCCGGGGCGCTCGCCGACGTCTAT
GGACGCAAGCGCCTGTTCACTGCCGGCATGCTGTTATTCACCGCAGCGTCGATTGCCCAGAGCCTGGCGCCTTCGGTGTT
CTGGCTGGATGTGCTTCGGGCGATTCAGGGCGTGGCCGGTGCGGCGGCGCTGGCCAGTGGTTCGGCGGCGTTGGCGCAGG
AATTCGAAGGCCATGCGCGCACTCGTGCGTACAGCATGCTCGGCGCCACGTTTGGCATCGGTCTCGCGTTCGGACCTCTG
GTGGCCGGGGCGTTGATCGAGGCGTTCAACTGGCGCGCGATCTTTGTGTTTACCGCGCTGATTGGTGTGATCGCGATGGT
CTTCGGCCTGCCGCGCATGCGTGAAACCCGTGACCCCGACGCCAGCGGTCTCGACTGGCCGGGTACCGTGACGTTCAGCT
CGATGCTGGCGCTGTTCACGTTCGGTGTGATCCAGGCGCCCGAAAGCGGTTGGGGCAGTCCTTTAGTGGTCGGTCTGTTG
GGTGCTTCGGCGTTGTTGCTGGCACTGTTCGTCTGCATCGAAATGCGCGTCAAACGTCCAATGCTCGATCTGACGCTGTT
CCGCTTTCCGCGCTTCGTCGGCGTGCAGATGCTGCCGATCGGCACCTGCTATTGCTACATCGTGCTGGTGGTCATGCTGC
CGCTGCGCTTTATCGGTGTCGAAGGCTTGAGTGAAATCGATGCCGGCCTGCTGTTGCTGGCGCTGTCGGCGCCGATGCTG
GTGGTGCCGATGCTGGCGGCGTCCATGACACGGTTTGTCTCGGCCGGCATCCTGTCGGGCGTGGGCTTCCTGATTGCCGC
CATGGGGCTGCACTGGCTGAGCCTGTACAACGTCGGCGAGCCGAAATTCGCGCTGGTGGTGCCGATGGTGTTGATCGGGA
TCGGCGCGGGTCTGCCGTGGGGCTTGATGGATGGACTGTCGGTCAGCGTGGTGCCGAAAGAGCGCGCCGGCATGGCGGCC
GGGATTTTCAACACGGTACGGGTGGCGGGTGAAGGGATTGCACTGGCGAGCGTGGCGGCGATTCTTGCCTCGCTGCTGCA
TACCGATCTGCAGGCTGTGGCCAACGGTGCAGATGCAGCGCTGATTGCCGAGACCGCGCACCGGGTGACCACTGGCGACA
TGGCCCATGCGATCAACACGGTTCCGGGCCTGGCCAATGAGCATCTGGTTCAAGGTTACGCTTCGGCGTTTCAGTACCTG
CTGCACATCCTGACGATCATCACGCTGGTGTCGGCGGCTGTGGTGCTGGGGCTATTAAGCAAAGATCGGACGGTGTCGGA
ACACCAACAGGCTCCGGCATAA

Upstream 100 bases:

>100_bases
AGCTGGCGCTGTCCACGGATTTCAAGGACTGACCTAGCCCCTCGTTGAAGCCATCGCAGGCTGCGCTCTGTTGATCGCAG
CCTTGTTTTCTATTCATTGG

Downstream 100 bases:

>100_bases
ATGAAAAAGCCCGGAAGCGCAAAACGCTTCCGGGCTTCTTTTCATTGGATCAGCGGCGATCCAGCCACACCGTCTGCGCG
TTGCAGAACTCGCGCACGCC

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MKSMTTVSQYQGAREKLLILAAICLSALVLPLSFTGGAVATPAIGRDLGGSPVALTWITNAFMLSFGSLLMAAGALADVY
GRKRLFTAGMLLFTAASIAQSLAPSVFWLDVLRAIQGVAGAAALASGSAALAQEFEGHARTRAYSMLGATFGIGLAFGPL
VAGALIEAFNWRAIFVFTALIGVIAMVFGLPRMRETRDPDASGLDWPGTVTFSSMLALFTFGVIQAPESGWGSPLVVGLL
GASALLLALFVCIEMRVKRPMLDLTLFRFPRFVGVQMLPIGTCYCYIVLVVMLPLRFIGVEGLSEIDAGLLLLALSAPML
VVPMLAASMTRFVSAGILSGVGFLIAAMGLHWLSLYNVGEPKFALVVPMVLIGIGAGLPWGLMDGLSVSVVPKERAGMAA
GIFNTVRVAGEGIALASVAAILASLLHTDLQAVANGADAALIAETAHRVTTGDMAHAINTVPGLANEHLVQGYASAFQYL
LHILTIITLVSAAVVLGLLSKDRTVSEHQQAPA

Sequences:

>Translated_513_residues
MKSMTTVSQYQGAREKLLILAAICLSALVLPLSFTGGAVATPAIGRDLGGSPVALTWITNAFMLSFGSLLMAAGALADVY
GRKRLFTAGMLLFTAASIAQSLAPSVFWLDVLRAIQGVAGAAALASGSAALAQEFEGHARTRAYSMLGATFGIGLAFGPL
VAGALIEAFNWRAIFVFTALIGVIAMVFGLPRMRETRDPDASGLDWPGTVTFSSMLALFTFGVIQAPESGWGSPLVVGLL
GASALLLALFVCIEMRVKRPMLDLTLFRFPRFVGVQMLPIGTCYCYIVLVVMLPLRFIGVEGLSEIDAGLLLLALSAPML
VVPMLAASMTRFVSAGILSGVGFLIAAMGLHWLSLYNVGEPKFALVVPMVLIGIGAGLPWGLMDGLSVSVVPKERAGMAA
GIFNTVRVAGEGIALASVAAILASLLHTDLQAVANGADAALIAETAHRVTTGDMAHAINTVPGLANEHLVQGYASAFQYL
LHILTIITLVSAAVVLGLLSKDRTVSEHQQAPA
>Mature_513_residues
MKSMTTVSQYQGAREKLLILAAICLSALVLPLSFTGGAVATPAIGRDLGGSPVALTWITNAFMLSFGSLLMAAGALADVY
GRKRLFTAGMLLFTAASIAQSLAPSVFWLDVLRAIQGVAGAAALASGSAALAQEFEGHARTRAYSMLGATFGIGLAFGPL
VAGALIEAFNWRAIFVFTALIGVIAMVFGLPRMRETRDPDASGLDWPGTVTFSSMLALFTFGVIQAPESGWGSPLVVGLL
GASALLLALFVCIEMRVKRPMLDLTLFRFPRFVGVQMLPIGTCYCYIVLVVMLPLRFIGVEGLSEIDAGLLLLALSAPML
VVPMLAASMTRFVSAGILSGVGFLIAAMGLHWLSLYNVGEPKFALVVPMVLIGIGAGLPWGLMDGLSVSVVPKERAGMAA
GIFNTVRVAGEGIALASVAAILASLLHTDLQAVANGADAALIAETAHRVTTGDMAHAINTVPGLANEHLVQGYASAFQYL
LHILTIITLVSAAVVLGLLSKDRTVSEHQQAPA

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=392, Percent_Identity=27.2959183673469, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=24.5614035087719, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788710, Length=430, Percent_Identity=23.4883720930233, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789042, Length=422, Percent_Identity=24.6445497630332, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=243, Percent_Identity=27.1604938271605, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53471; Mature: 53471

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMTTVSQYQGAREKLLILAAICLSALVLPLSFTGGAVATPAIGRDLGGSPVALTWITN
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHH
AFMLSFGSLLMAAGALADVYGRKRLFTAGMLLFTAASIAQSLAPSVFWLDVLRAIQGVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AAALASGSAALAQEFEGHARTRAYSMLGATFGIGLAFGPLVAGALIEAFNWRAIFVFTAL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IGVIAMVFGLPRMRETRDPDASGLDWPGTVTFSSMLALFTFGVIQAPESGWGSPLVVGLL
HHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GASALLLALFVCIEMRVKRPMLDLTLFRFPRFVGVQMLPIGTCYCYIVLVVMLPLRFIGV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
EGLSEIDAGLLLLALSAPMLVVPMLAASMTRFVSAGILSGVGFLIAAMGLHWLSLYNVGE
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKFALVVPMVLIGIGAGLPWGLMDGLSVSVVPKERAGMAAGIFNTVRVAGEGIALASVAA
CHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECHHHCCHHHHHHHHHHHHCCCHHHHHHHH
ILASLLHTDLQAVANGADAALIAETAHRVTTGDMAHAINTVPGLANEHLVQGYASAFQYL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LHILTIITLVSAAVVLGLLSKDRTVSEHQQAPA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure
MKSMTTVSQYQGAREKLLILAAICLSALVLPLSFTGGAVATPAIGRDLGGSPVALTWITN
CCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHHH
AFMLSFGSLLMAAGALADVYGRKRLFTAGMLLFTAASIAQSLAPSVFWLDVLRAIQGVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AAALASGSAALAQEFEGHARTRAYSMLGATFGIGLAFGPLVAGALIEAFNWRAIFVFTAL
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
IGVIAMVFGLPRMRETRDPDASGLDWPGTVTFSSMLALFTFGVIQAPESGWGSPLVVGLL
HHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GASALLLALFVCIEMRVKRPMLDLTLFRFPRFVGVQMLPIGTCYCYIVLVVMLPLRFIGV
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
EGLSEIDAGLLLLALSAPMLVVPMLAASMTRFVSAGILSGVGFLIAAMGLHWLSLYNVGE
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKFALVVPMVLIGIGAGLPWGLMDGLSVSVVPKERAGMAAGIFNTVRVAGEGIALASVAA
CHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECHHHCCHHHHHHHHHHHHCCCHHHHHHHH
ILASLLHTDLQAVANGADAALIAETAHRVTTGDMAHAINTVPGLANEHLVQGYASAFQYL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LHILTIITLVSAAVVLGLLSKDRTVSEHQQAPA
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]