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The map label for this gene is yneJ [H]

Identifier: 77459497

GI number: 77459497

Start: 3757159

End: 3758046

Strand: Reverse

Name: yneJ [H]

Synonym: Pfl01_3275

Alternate gene names: 77459497

Gene position: 3758046-3757159 (Counterclockwise)

Preceding gene: 77459499

Following gene: 77459496

Centisome position: 58.37

GC content: 62.39

Gene sequence:

>888_bases
ATGACTGTGGATCTGGTGCAACTGGAAATCTTCAAGGCCGTTGCCGAGCACGGCAGCATCAGTGCTGCGGCGGCGCAGAT
CCATCGCGTGCCGTCGAACCTGACGACGCGCATCAAGCAGTTGGAGGAGGATCTAGGCGTCGACCTGTTCATCCGCGAGA
AAAGCCGTTTGCGCCTGTCGCCGGCCGGCTGGAGTTTTCTTGAATACGCGCGGCGCATTCTCGACCTGGTCCAGGAAGCC
CGCGCCACGGTGGCCGGCGAGGAACCGCAAGGGGCGTTCCCGCTGGGTTCGCTCGAAAGCACGGCGGCGGTGCGGATTCC
CGGTTTGCTGGCGACCTATAACCAGCAACATCCCAAGGTCGATCTGGACCTGTCGACCGGTCCGTCCGGGACGATGATCG
ACGGTGTGCTTTCCGGTCGCCTGGCGGCCGCGTTCGTCGACGGCCCGGTCCTGCATCCGGCGCTGGAAGGCATTCCGGCG
TTCGAAGAGGAAATGGTCATCATCTCTCCACTTCATCACGGGCCGATCCAGCGCGCTGCGGACGTGAATGGCGAGAACAT
CTATGCATTCCGTTCGAACTGCTCCTATCGCCATCACTTCGAAAAATGGTTCAGCACCGATGCTGCCGTGCCCGGCAAGA
TCTTCGAAATGGAGTCTTATCACGGCATGCTCGCCTGTGTCAGTGCCGGCGCCGGCCTGGCCCTGATGCCACGCAGCATG
CTGCAAAGCATGCCCGGCTGCGCGACGGTCAGCGTATGGCCGCTGGCGTCGGATTTCCGCTATCTGACGACGTGGCTGGT
ATGGCGGCGGGGCACGGTTTCGCGCAGTCTGAGCATGTTTGTGCGCTTGCTGGAAGAGCGCGGTGTGGTGCGCGCAGAAG
AGAATTGA

Upstream 100 bases:

>100_bases
CATGGCATCGTCCTGCGAGGTCAGTGGATGCGCTCAGAGTAGGGTGGCGTTTGGTTTCTGGAAACTGAATAATAAAGAGC
GTTTCTTTCACGATTGGAGA

Downstream 100 bases:

>100_bases
CACGTTGTGTCATGGTTGTATCTTAGGCGTCCTGTTTCGCAATATAAAAAACTATACAGCGCAAGCGATTAGCCAGAAGG
AATCAGCGATGACCACAGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MTVDLVQLEIFKAVAEHGSISAAAAQIHRVPSNLTTRIKQLEEDLGVDLFIREKSRLRLSPAGWSFLEYARRILDLVQEA
RATVAGEEPQGAFPLGSLESTAAVRIPGLLATYNQQHPKVDLDLSTGPSGTMIDGVLSGRLAAAFVDGPVLHPALEGIPA
FEEEMVIISPLHHGPIQRAADVNGENIYAFRSNCSYRHHFEKWFSTDAAVPGKIFEMESYHGMLACVSAGAGLALMPRSM
LQSMPGCATVSVWPLASDFRYLTTWLVWRRGTVSRSLSMFVRLLEERGVVRAEEN

Sequences:

>Translated_295_residues
MTVDLVQLEIFKAVAEHGSISAAAAQIHRVPSNLTTRIKQLEEDLGVDLFIREKSRLRLSPAGWSFLEYARRILDLVQEA
RATVAGEEPQGAFPLGSLESTAAVRIPGLLATYNQQHPKVDLDLSTGPSGTMIDGVLSGRLAAAFVDGPVLHPALEGIPA
FEEEMVIISPLHHGPIQRAADVNGENIYAFRSNCSYRHHFEKWFSTDAAVPGKIFEMESYHGMLACVSAGAGLALMPRSM
LQSMPGCATVSVWPLASDFRYLTTWLVWRRGTVSRSLSMFVRLLEERGVVRAEEN
>Mature_294_residues
TVDLVQLEIFKAVAEHGSISAAAAQIHRVPSNLTTRIKQLEEDLGVDLFIREKSRLRLSPAGWSFLEYARRILDLVQEAR
ATVAGEEPQGAFPLGSLESTAAVRIPGLLATYNQQHPKVDLDLSTGPSGTMIDGVLSGRLAAAFVDGPVLHPALEGIPAF
EEEMVIISPLHHGPIQRAADVNGENIYAFRSNCSYRHHFEKWFSTDAAVPGKIFEMESYHGMLACVSAGAGLALMPRSML
QSMPGCATVSVWPLASDFRYLTTWLVWRRGTVSRSLSMFVRLLEERGVVRAEEN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=66.0777385159011, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=23.728813559322, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=26.2948207171315, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI157672245, Length=255, Percent_Identity=24.7058823529412, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788481, Length=180, Percent_Identity=29.4444444444444, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082132, Length=297, Percent_Identity=24.5791245791246, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790399, Length=280, Percent_Identity=23.9285714285714, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790262, Length=249, Percent_Identity=24.0963855421687, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=155, Percent_Identity=29.0322580645161, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI145693105, Length=279, Percent_Identity=21.1469534050179, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789639, Length=243, Percent_Identity=25.1028806584362, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788706, Length=147, Percent_Identity=27.891156462585, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32386; Mature: 32254

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDLVQLEIFKAVAEHGSISAAAAQIHRVPSNLTTRIKQLEEDLGVDLFIREKSRLRLS
CCEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEEC
PAGWSFLEYARRILDLVQEARATVAGEEPQGAFPLGSLESTAAVRIPGLLATYNQQHPKV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCEE
DLDLSTGPSGTMIDGVLSGRLAAAFVDGPVLHPALEGIPAFEEEMVIISPLHHGPIQRAA
EEEECCCCCCCHHHHHHHCHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHH
DVNGENIYAFRSNCSYRHHFEKWFSTDAAVPGKIFEMESYHGMLACVSAGAGLALMPRSM
CCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEHHHCHHHHHHHCCCCCEECCHHH
LQSMPGCATVSVWPLASDFRYLTTWLVWRRGTVSRSLSMFVRLLEERGVVRAEEN
HHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TVDLVQLEIFKAVAEHGSISAAAAQIHRVPSNLTTRIKQLEEDLGVDLFIREKSRLRLS
CEEHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCEEEC
PAGWSFLEYARRILDLVQEARATVAGEEPQGAFPLGSLESTAAVRIPGLLATYNQQHPKV
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCEE
DLDLSTGPSGTMIDGVLSGRLAAAFVDGPVLHPALEGIPAFEEEMVIISPLHHGPIQRAA
EEEECCCCCCCHHHHHHHCHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHHH
DVNGENIYAFRSNCSYRHHFEKWFSTDAAVPGKIFEMESYHGMLACVSAGAGLALMPRSM
CCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEHHHCHHHHHHHCCCCCEECCHHH
LQSMPGCATVSVWPLASDFRYLTTWLVWRRGTVSRSLSMFVRLLEERGVVRAEEN
HHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]