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The map label for this gene is potH [H]

Identifier: 77459464

GI number: 77459464

Start: 3723042

End: 3723986

Strand: Direct

Name: potH [H]

Synonym: Pfl01_3242

Alternate gene names: 77459464

Gene position: 3723042-3723986 (Clockwise)

Preceding gene: 77459463

Following gene: 77459465

Centisome position: 57.83

GC content: 64.02

Gene sequence:

>945_bases
ATGAAAATGGCGGCCACCGCATCCCGTCCCTCCACTGCCACCGGGAGCGCCCCGAGCGCTGCCGGCCTGGCATCCGGCAA
GGCGAGTGCAATGGCGCACGCCCCGTCCCTCAAGCAACGCTGGCGCGGCGCCGGCAACCTCGCGCCGGCGCTGTTGTTCC
TCGGTCTGTTCTTTCTCGCCCCGCTGATTGGTTTGTTGCTGCGCGGCGTGCTGGAACCGGTGCCGGGCCTCGGCAACTAT
GAGCAACTGTTCGCTAACTCGGCCTACGCCCGGGTGCTGTTCAACACCTTTTCGGTAGCGGGATTGGTGACGGTGTTCAG
CCTGCTGCTGGGCTTTCCGCTGGCCTGGGCAATCACCCTGGTGCCGCGTGGCTGGGGCCGCTGGATCCTCAACATCGTGC
TGCTGTCGATGTGGACCAGCCTCCTCGCCCGCACCTATTCGTGGCTGGTGCTGTTGCAGGCTTCGGGTGTCATCAATAAA
GCCTTGATGGCGATGGGCATCATCGATCAGCCGCTGGAAATGGTGCACAACCTGACCGGCGTGGTGATCGGCATGAGCTA
CATCATGATTCCGTTCATCGTGCTGCCGTTGCAGGCGACCATGCAGGCCATCGACCCGATGATCCTGCAGGCCGGCTCGA
TCTGCGGCGCGAGCCCGTGGACCAACTTCTTTCGGGTGTTCCTGCCGCTGTGCCGGCCGGGTCTGGCGTCCGGCGGCTTG
ATGGTGTTCGTGATGTCGCTCGGTTACTACGTCACCCCGGCGCTGCTGGGCGGGGCGCAGAACATGATGCTGCCGGAATT
CATCATTCAGCAGGTGCAGTCGTTCCTCAATTGGGGCCTGGCCAGCGCCGGCGCCGCGTTGCTGATCGTCATCACGCTGG
TGCTGTTCTACGTCTACCTGAAGCTGCAGCCGGAATCCCCGGTTGGCGCCAGTCACGCGAGGTAA

Upstream 100 bases:

>100_bases
TGCCGACTGCCTACGCTAAGGATCAGATCACTCTTGATTTCGCGTACTGGGCCAAGAACGGTCCGGCCATCGCGACACGG
TGGAACGAATGGCTGGTCAA

Downstream 100 bases:

>100_bases
GCCGACATGCTCCTGACGCCCAATGCCATGAGTCGCCGGATGCGCTTCGGCCTGTATTTCACCACCGGGTTGATCGGCCT
GTTCCTGCTGTTGCCGATCG

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKMAATASRPSTATGSAPSAAGLASGKASAMAHAPSLKQRWRGAGNLAPALLFLGLFFLAPLIGLLLRGVLEPVPGLGNY
EQLFANSAYARVLFNTFSVAGLVTVFSLLLGFPLAWAITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGVINK
ALMAMGIIDQPLEMVHNLTGVVIGMSYIMIPFIVLPLQATMQAIDPMILQAGSICGASPWTNFFRVFLPLCRPGLASGGL
MVFVMSLGYYVTPALLGGAQNMMLPEFIIQQVQSFLNWGLASAGAALLIVITLVLFYVYLKLQPESPVGASHAR

Sequences:

>Translated_314_residues
MKMAATASRPSTATGSAPSAAGLASGKASAMAHAPSLKQRWRGAGNLAPALLFLGLFFLAPLIGLLLRGVLEPVPGLGNY
EQLFANSAYARVLFNTFSVAGLVTVFSLLLGFPLAWAITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGVINK
ALMAMGIIDQPLEMVHNLTGVVIGMSYIMIPFIVLPLQATMQAIDPMILQAGSICGASPWTNFFRVFLPLCRPGLASGGL
MVFVMSLGYYVTPALLGGAQNMMLPEFIIQQVQSFLNWGLASAGAALLIVITLVLFYVYLKLQPESPVGASHAR
>Mature_314_residues
MKMAATASRPSTATGSAPSAAGLASGKASAMAHAPSLKQRWRGAGNLAPALLFLGLFFLAPLIGLLLRGVLEPVPGLGNY
EQLFANSAYARVLFNTFSVAGLVTVFSLLLGFPLAWAITLVPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGVINK
ALMAMGIIDQPLEMVHNLTGVVIGMSYIMIPFIVLPLQATMQAIDPMILQAGSICGASPWTNFFRVFLPLCRPGLASGGL
MVFVMSLGYYVTPALLGGAQNMMLPEFIIQQVQSFLNWGLASAGAALLIVITLVLFYVYLKLQPESPVGASHAR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=231, Percent_Identity=35.0649350649351, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1787369, Length=223, Percent_Identity=30.4932735426009, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787713, Length=229, Percent_Identity=30.5676855895196, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33555; Mature: 33555

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMAATASRPSTATGSAPSAAGLASGKASAMAHAPSLKQRWRGAGNLAPALLFLGLFFLA
CCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHH
PLIGLLLRGVLEPVPGLGNYEQLFANSAYARVLFNTFSVAGLVTVFSLLLGFPLAWAITL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGVINKALMAMGIIDQPLEMVHNLTG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVIGMSYIMIPFIVLPLQATMQAIDPMILQAGSICGASPWTNFFRVFLPLCRPGLASGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
MVFVMSLGYYVTPALLGGAQNMMLPEFIIQQVQSFLNWGLASAGAALLIVITLVLFYVYL
HHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KLQPESPVGASHAR
HCCCCCCCCCCCCC
>Mature Secondary Structure
MKMAATASRPSTATGSAPSAAGLASGKASAMAHAPSLKQRWRGAGNLAPALLFLGLFFLA
CCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHH
PLIGLLLRGVLEPVPGLGNYEQLFANSAYARVLFNTFSVAGLVTVFSLLLGFPLAWAITL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPRGWGRWILNIVLLSMWTSLLARTYSWLVLLQASGVINKALMAMGIIDQPLEMVHNLTG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVIGMSYIMIPFIVLPLQATMQAIDPMILQAGSICGASPWTNFFRVFLPLCRPGLASGGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
MVFVMSLGYYVTPALLGGAQNMMLPEFIIQQVQSFLNWGLASAGAALLIVITLVLFYVYL
HHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KLQPESPVGASHAR
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]