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The map label for this gene is potC [H]

Identifier: 77459465

GI number: 77459465

Start: 3723993

End: 3724802

Strand: Direct

Name: potC [H]

Synonym: Pfl01_3243

Alternate gene names: 77459465

Gene position: 3723993-3724802 (Clockwise)

Preceding gene: 77459464

Following gene: 77459466

Centisome position: 57.84

GC content: 63.21

Gene sequence:

>810_bases
ATGCTCCTGACGCCCAATGCCATGAGTCGCCGGATGCGCTTCGGCCTGTATTTCACCACCGGGTTGATCGGCCTGTTCCT
GCTGTTGCCGATCGTGTTCATCGTCCTGCTGTCGTTCGGTTCGTCCCAGTGGCTGGTGTTCCCGCCACCGGGCTGGACGC
TGAAATGGTACGTGCAGTTTTTCTCCAATGCCGACTGGATGAGCGCAGCGCTGGCCAGCCTCAAGGTCGCGGTGCTGACC
ACGATCTGCGCCGTCGCCCTCGGTTTGCCGACGGCCTTTGCGCTGGTGCGCGGACGCTTCCCCGGCCGGGAAATGCTCTA
CGGCCTGTTCACCCTGCCGATGATCGTGCCGCTGGTGATCATCGCCGTGGCGGTGTACGCGCTGTTCCTCAAGCTCGGCT
ACACCGGCACGATGTTCGCCTTCGTCGTCAGCCACGTGATCGTCGCGCTGCCGTTCACCATCATCTCGATCATCAATTCG
CTGAAGCTGTTCGATCAGTCGATTGAAGACGCGGCGGTGATCTGCGGTGCGTCACGCCTGCAAGCGGTGTTCAAGGTGAC
CTTCCCGGCGATCCGTCCGGGGATGGTGGCCGGCGCCCTCTTCGCCTTCCTGGTTTCGTGGGACGAAGTGGTGCTCAGCG
TGATGATGGCCAGCCCGACCCTGCAAACCCTTCCCGTGAAAATGTGGACCACCCTGCGCCAAGACCTGACGCCCGTGATC
GCCGTCGCCTCGACGCTGCTGATCGGCCTGTCGGTATTGGTCATGGTGATCGCCGCCGCCCTGCGCCGGCGCAACGAAAT
CAGCGCCTGA

Upstream 100 bases:

>100_bases
CGCCGCGTTGCTGATCGTCATCACGCTGGTGCTGTTCTACGTCTACCTGAAGCTGCAGCCGGAATCCCCGGTTGGCGCCA
GTCACGCGAGGTAAGCCGAC

Downstream 100 bases:

>100_bases
GCGCCCAGGAGTACGACATGAGTGCCGTGATCAAAGACGCATCCCAGCAGAACGACAAACCCCTGGTCAGCCTGCGCAAC
CTGAACAAGCACTACGGCGA

Product: binding-protein dependent transport system inner membrane protein

Products: Phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MLLTPNAMSRRMRFGLYFTTGLIGLFLLLPIVFIVLLSFGSSQWLVFPPPGWTLKWYVQFFSNADWMSAALASLKVAVLT
TICAVALGLPTAFALVRGRFPGREMLYGLFTLPMIVPLVIIAVAVYALFLKLGYTGTMFAFVVSHVIVALPFTIISIINS
LKLFDQSIEDAAVICGASRLQAVFKVTFPAIRPGMVAGALFAFLVSWDEVVLSVMMASPTLQTLPVKMWTTLRQDLTPVI
AVASTLLIGLSVLVMVIAAALRRRNEISA

Sequences:

>Translated_269_residues
MLLTPNAMSRRMRFGLYFTTGLIGLFLLLPIVFIVLLSFGSSQWLVFPPPGWTLKWYVQFFSNADWMSAALASLKVAVLT
TICAVALGLPTAFALVRGRFPGREMLYGLFTLPMIVPLVIIAVAVYALFLKLGYTGTMFAFVVSHVIVALPFTIISIINS
LKLFDQSIEDAAVICGASRLQAVFKVTFPAIRPGMVAGALFAFLVSWDEVVLSVMMASPTLQTLPVKMWTTLRQDLTPVI
AVASTLLIGLSVLVMVIAAALRRRNEISA
>Mature_269_residues
MLLTPNAMSRRMRFGLYFTTGLIGLFLLLPIVFIVLLSFGSSQWLVFPPPGWTLKWYVQFFSNADWMSAALASLKVAVLT
TICAVALGLPTAFALVRGRFPGREMLYGLFTLPMIVPLVIIAVAVYALFLKLGYTGTMFAFVVSHVIVALPFTIISIINS
LKLFDQSIEDAAVICGASRLQAVFKVTFPAIRPGMVAGALFAFLVSWDEVVLSVMMASPTLQTLPVKMWTTLRQDLTPVI
AVASTLLIGLSVLVMVIAAALRRRNEISA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787368, Length=229, Percent_Identity=33.1877729257642, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787081, Length=248, Percent_Identity=32.6612903225806, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787714, Length=244, Percent_Identity=28.6885245901639, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787571, Length=227, Percent_Identity=25.9911894273128, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788764, Length=170, Percent_Identity=28.8235294117647, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29369; Mature: 29369

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLTPNAMSRRMRFGLYFTTGLIGLFLLLPIVFIVLLSFGSSQWLVFPPPGWTLKWYVQF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
FSNADWMSAALASLKVAVLTTICAVALGLPTAFALVRGRFPGREMLYGLFTLPMIVPLVI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAVAVYALFLKLGYTGTMFAFVVSHVIVALPFTIISIINSLKLFDQSIEDAAVICGASRL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVFKVTFPAIRPGMVAGALFAFLVSWDEVVLSVMMASPTLQTLPVKMWTTLRQDLTPVI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
AVASTLLIGLSVLVMVIAAALRRRNEISA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]