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The map label for this gene is potD-B [H]

Identifier: 77459463

GI number: 77459463

Start: 3722020

End: 3723045

Strand: Direct

Name: potD-B [H]

Synonym: Pfl01_3241

Alternate gene names: 77459463

Gene position: 3722020-3723045 (Clockwise)

Preceding gene: 77459462

Following gene: 77459464

Centisome position: 57.81

GC content: 62.48

Gene sequence:

>1026_bases
ATGGTGTTGAACAAAGCTGCAACCGCGATCTTTCTTGCGGGACTGCTGAGCGTGACCGGTCAGGCCGCGATGGCCGCCGA
GAGTGTGAACTTTGTCAGCTGGGGCGGTAGCACCCAGGATGCGCAGAAACAGGCCTGGGCCGATCCGTTCAGCAAGGCCA
GCGGCATCACCGTCGTGCAGGACGGCCCGACCGACTACGGCAAACTCAAGGCCATGGTCGAAAGCGGCAACGTGCAATGG
GACGTGGTCGACGTCGAAGCCGACTTCGCTCTGCGCGCCGCCGCCGAAGGCCTGCTCGAACCCCTCGATTTCAAACAGAT
CCAGCGCGACAAGATCGACCCGCGTTTTGTCAGCGACCACGGCGTCGGCTCGTTCTTCTTCTCCTTCGTCCTCGGCTACA
ACGAGGGCAAACTCGGCGCCAACAAGCCGCAGGACTGGAGCGCGCTGTTCGACACCAAGACCTTCCCCGGCAAACGCGCC
CTCTATAAATGGCCGAGCCCCGGCGTACTGGAACTGGCCCTGCTCGCCGATGGCGTAGCGGCCGACAAGCTTTACCCGCT
GGACTTGGATCGCGCCTTCAAGAAACTCGACACCATCAAGAAAGACATCGTCTGGTGGGGCGGCGGCGCGCAGTCGCAGC
AACTGCTGGCCTCCGGTGAAGCGAGCATGGGCCAGTTCTGGAACGGCCGGATTCATGCCCTGCAGGAAGACGGCGCACCG
GTCGGCGTGAGCTGGAAACAGAACCTGGTCATGGCCGACATTCTGGTCGTGCCAAAGGGCTCGAAAAACAAGGACGCGGC
GATGAAGTTCCTGGCCAGCGCCAGTAGCGCGAAGGGCCAGGCCGACTTCTCCAACCTGACCGCTTACGCCCCGGTCAACG
TCGACAGCGTGGCGCGTCTGGACTCGACGCTGGCCCCGAACCTGCCGACTGCCTACGCTAAGGATCAGATCACTCTTGAT
TTCGCGTACTGGGCCAAGAACGGTCCGGCCATCGCGACACGGTGGAACGAATGGCTGGTCAAATGA

Upstream 100 bases:

>100_bases
TTCCATAGACCGGACGATCTGAAAAACCGTCCAGCTACTGCTCCCGACAGGCGGGCAACAACGCAAGCCCGCTCAAAAAC
ACAACAATGAGGGCGTAAAA

Downstream 100 bases:

>100_bases
AAATGGCGGCCACCGCATCCCGTCCCTCCACTGCCACCGGGAGCGCCCCGAGCGCTGCCGGCCTGGCATCCGGCAAGGCG
AGTGCAATGGCGCACGCCCC

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MVLNKAATAIFLAGLLSVTGQAAMAAESVNFVSWGGSTQDAQKQAWADPFSKASGITVVQDGPTDYGKLKAMVESGNVQW
DVVDVEADFALRAAAEGLLEPLDFKQIQRDKIDPRFVSDHGVGSFFFSFVLGYNEGKLGANKPQDWSALFDTKTFPGKRA
LYKWPSPGVLELALLADGVAADKLYPLDLDRAFKKLDTIKKDIVWWGGGAQSQQLLASGEASMGQFWNGRIHALQEDGAP
VGVSWKQNLVMADILVVPKGSKNKDAAMKFLASASSAKGQADFSNLTAYAPVNVDSVARLDSTLAPNLPTAYAKDQITLD
FAYWAKNGPAIATRWNEWLVK

Sequences:

>Translated_341_residues
MVLNKAATAIFLAGLLSVTGQAAMAAESVNFVSWGGSTQDAQKQAWADPFSKASGITVVQDGPTDYGKLKAMVESGNVQW
DVVDVEADFALRAAAEGLLEPLDFKQIQRDKIDPRFVSDHGVGSFFFSFVLGYNEGKLGANKPQDWSALFDTKTFPGKRA
LYKWPSPGVLELALLADGVAADKLYPLDLDRAFKKLDTIKKDIVWWGGGAQSQQLLASGEASMGQFWNGRIHALQEDGAP
VGVSWKQNLVMADILVVPKGSKNKDAAMKFLASASSAKGQADFSNLTAYAPVNVDSVARLDSTLAPNLPTAYAKDQITLD
FAYWAKNGPAIATRWNEWLVK
>Mature_341_residues
MVLNKAATAIFLAGLLSVTGQAAMAAESVNFVSWGGSTQDAQKQAWADPFSKASGITVVQDGPTDYGKLKAMVESGNVQW
DVVDVEADFALRAAAEGLLEPLDFKQIQRDKIDPRFVSDHGVGSFFFSFVLGYNEGKLGANKPQDWSALFDTKTFPGKRA
LYKWPSPGVLELALLADGVAADKLYPLDLDRAFKKLDTIKKDIVWWGGGAQSQQLLASGEASMGQFWNGRIHALQEDGAP
VGVSWKQNLVMADILVVPKGSKNKDAAMKFLASASSAKGQADFSNLTAYAPVNVDSVARLDSTLAPNLPTAYAKDQITLD
FAYWAKNGPAIATRWNEWLVK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36827; Mature: 36827

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLNKAATAIFLAGLLSVTGQAAMAAESVNFVSWGGSTQDAQKQAWADPFSKASGITVVQ
CCCCHHHHHHHHHHHHHHCCHHHHHHHCCEEEECCCCCCHHHHHHHCCCHHHCCCEEEEE
DGPTDYGKLKAMVESGNVQWDVVDVEADFALRAAAEGLLEPLDFKQIQRDKIDPRFVSDH
CCCCCHHHHHHEEECCCEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCC
GVGSFFFSFVLGYNEGKLGANKPQDWSALFDTKTFPGKRALYKWPSPGVLELALLADGVA
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCEEEEEHHCCCC
ADKLYPLDLDRAFKKLDTIKKDIVWWGGGAQSQQLLASGEASMGQFWNGRIHALQEDGAP
CCCEECCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCCHHCCCCCCEEEEEECCCCC
VGVSWKQNLVMADILVVPKGSKNKDAAMKFLASASSAKGQADFSNLTAYAPVNVDSVARL
CCCCHHCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHH
DSTLAPNLPTAYAKDQITLDFAYWAKNGPAIATRWNEWLVK
HHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEHHHHCCC
>Mature Secondary Structure
MVLNKAATAIFLAGLLSVTGQAAMAAESVNFVSWGGSTQDAQKQAWADPFSKASGITVVQ
CCCCHHHHHHHHHHHHHHCCHHHHHHHCCEEEECCCCCCHHHHHHHCCCHHHCCCEEEEE
DGPTDYGKLKAMVESGNVQWDVVDVEADFALRAAAEGLLEPLDFKQIQRDKIDPRFVSDH
CCCCCHHHHHHEEECCCEEEEEEEECCCHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCC
GVGSFFFSFVLGYNEGKLGANKPQDWSALFDTKTFPGKRALYKWPSPGVLELALLADGVA
CHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCEEEEEHHCCCC
ADKLYPLDLDRAFKKLDTIKKDIVWWGGGAQSQQLLASGEASMGQFWNGRIHALQEDGAP
CCCEECCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHCCCCHHCCCCCCEEEEEECCCCC
VGVSWKQNLVMADILVVPKGSKNKDAAMKFLASASSAKGQADFSNLTAYAPVNVDSVARL
CCCCHHCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHH
DSTLAPNLPTAYAKDQITLDFAYWAKNGPAIATRWNEWLVK
HHHCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]