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The map label for this gene is rutD [H]

Identifier: 77458702

GI number: 77458702

Start: 2832285

End: 2833154

Strand: Reverse

Name: rutD [H]

Synonym: Pfl01_2477

Alternate gene names: 77458702

Gene position: 2833154-2832285 (Counterclockwise)

Preceding gene: 77458703

Following gene: 77458701

Centisome position: 44.0

GC content: 60.34

Gene sequence:

>870_bases
ATGAGTTCAATGCAGACAAATTCCCGCTCGGGTTCGCCGACGTCATTCATCGACGCGGCGAACCAGTCGATCACGGTCAA
CGGCATTCCCTTTGCCTACCGCGACACCGGGCCCACAACCGGCGTGCCGCTTGTACTGTTCAACCATTGGGGCGCGGTGC
TGGACAACTTCGACCCCGCGATCATCGATGGCCTGGCGCAAACCAGACGCGTCATCACCACCGACTATCGCGGCATCGGC
AACTCGGGTGGGACCGCGCCACTGACGGTGGGTGAAATGGCCGACGATGCCATCCAGCTGATCAGGGCGCTGGGGCTGAA
AACCGTGGATGTGCTCGGTTTTTCCCTCGGAGGTTTCGTCGCTCAGGACATTGCCCTCAAGGCGCCTGATTTGGTACGCC
GGTTGATTCTGACCGGTACTGGACCGGCGGGCGGCACCGGCATCGACAAGGTCGGCAGAGTGTCATGGCCATTGATCCTC
AAAGGCTTGCTGACCCTGCGTGATCCCAAGTTCTACCTTTTCTTCACCTCGTCGGCCAATGGGCGACGGTCTGCCTCGCA
GTATTTAAGACGCCTCAAGGCACGCAAGAACAATCGCGACAAGGGCCCGACGCCACGCGCCTTCCTGCGGCAATTGAAAG
CCATCACGGCGTGGGGCAGACAACCGCGGCAAGCGCTCGGACGCTTGCGCACACCGACACTGGTCGTCAACGGCGACAAC
GACATCATGGTGCCCAGCGTTAACTCGTTTGAACTGGCCAGGCTTATCCCCGACGCACAGTTGGTGATTTATGAGGATGC
CGGGCACGGCGGGATTTTCCAGCATCACGCTGACTTTGTGGCCAAGGCGCAGGCGTTTCTCGATGCTTGA

Upstream 100 bases:

>100_bases
TATCAGACGCCACAGTGATTTTTTGCTAGCGAGCATTCGCGCCGGGTTCGATGCAGGCACATGGCATTGCATCGCATCCT
GCCGGCTTGCCGGAGTGACA

Downstream 100 bases:

>100_bases
TCACGTCACGCAGCAACACCGCAACCCACCGACTACCCTCCGTACAACCAGCAAGAGCCGCACCACGATGAAAGCATTTC
TGATTGATGGCTATGGCAAG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: Aminohydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSSMQTNSRSGSPTSFIDAANQSITVNGIPFAYRDTGPTTGVPLVLFNHWGAVLDNFDPAIIDGLAQTRRVITTDYRGIG
NSGGTAPLTVGEMADDAIQLIRALGLKTVDVLGFSLGGFVAQDIALKAPDLVRRLILTGTGPAGGTGIDKVGRVSWPLIL
KGLLTLRDPKFYLFFTSSANGRRSASQYLRRLKARKNNRDKGPTPRAFLRQLKAITAWGRQPRQALGRLRTPTLVVNGDN
DIMVPSVNSFELARLIPDAQLVIYEDAGHGGIFQHHADFVAKAQAFLDA

Sequences:

>Translated_289_residues
MSSMQTNSRSGSPTSFIDAANQSITVNGIPFAYRDTGPTTGVPLVLFNHWGAVLDNFDPAIIDGLAQTRRVITTDYRGIG
NSGGTAPLTVGEMADDAIQLIRALGLKTVDVLGFSLGGFVAQDIALKAPDLVRRLILTGTGPAGGTGIDKVGRVSWPLIL
KGLLTLRDPKFYLFFTSSANGRRSASQYLRRLKARKNNRDKGPTPRAFLRQLKAITAWGRQPRQALGRLRTPTLVVNGDN
DIMVPSVNSFELARLIPDAQLVIYEDAGHGGIFQHHADFVAKAQAFLDA
>Mature_288_residues
SSMQTNSRSGSPTSFIDAANQSITVNGIPFAYRDTGPTTGVPLVLFNHWGAVLDNFDPAIIDGLAQTRRVITTDYRGIGN
SGGTAPLTVGEMADDAIQLIRALGLKTVDVLGFSLGGFVAQDIALKAPDLVRRLILTGTGPAGGTGIDKVGRVSWPLILK
GLLTLRDPKFYLFFTSSANGRRSASQYLRRLKARKNNRDKGPTPRAFLRQLKAITAWGRQPRQALGRLRTPTLVVNGDND
IMVPSVNSFELARLIPDAQLVIYEDAGHGGIFQHHADFVAKAQAFLDA

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR019913 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31169; Mature: 31038

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSMQTNSRSGSPTSFIDAANQSITVNGIPFAYRDTGPTTGVPLVLFNHWGAVLDNFDPA
CCCCCCCCCCCCCHHHHHCCCCEEEECCCCEEECCCCCCCCCCEEEEHHHHHHHCCCCHH
IIDGLAQTRRVITTDYRGIGNSGGTAPLTVGEMADDAIQLIRALGLKTVDVLGFSLGGFV
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AQDIALKAPDLVRRLILTGTGPAGGTGIDKVGRVSWPLILKGLLTLRDPKFYLFFTSSAN
HHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCC
GRRSASQYLRRLKARKNNRDKGPTPRAFLRQLKAITAWGRQPRQALGRLRTPTLVVNGDN
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCC
DIMVPSVNSFELARLIPDAQLVIYEDAGHGGIFQHHADFVAKAQAFLDA
CEEECCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSMQTNSRSGSPTSFIDAANQSITVNGIPFAYRDTGPTTGVPLVLFNHWGAVLDNFDPA
CCCCCCCCCCCCHHHHHCCCCEEEECCCCEEECCCCCCCCCCEEEEHHHHHHHCCCCHH
IIDGLAQTRRVITTDYRGIGNSGGTAPLTVGEMADDAIQLIRALGLKTVDVLGFSLGGFV
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AQDIALKAPDLVRRLILTGTGPAGGTGIDKVGRVSWPLILKGLLTLRDPKFYLFFTSSAN
HHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCC
GRRSASQYLRRLKARKNNRDKGPTPRAFLRQLKAITAWGRQPRQALGRLRTPTLVVNGDN
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCC
DIMVPSVNSFELARLIPDAQLVIYEDAGHGGIFQHHADFVAKAQAFLDA
CEEECCCCCHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA