Click here to switch to the map view.

The map label for this gene is mlhB [H]

Identifier: 77458466

GI number: 77458466

Start: 2569326

End: 2570294

Strand: Direct

Name: mlhB [H]

Synonym: Pfl01_2239

Alternate gene names: 77458466

Gene position: 2569326-2570294 (Clockwise)

Preceding gene: 77458464

Following gene: 77458468

Centisome position: 39.91

GC content: 61.82

Gene sequence:

>969_bases
ATGAAGAAATCAATGGACTGGCCCGCAGATGCTAACGAGTTGGCGCAATTGCGCGCGTTCAACCGCAAGCTTGCCCGGCT
GCCGCGCTTTCGCATCCGCAATCGCATCACGCCACGGGCGATTCAGTTGCTGTTGCGGCTAAGCCAATTGGGCGGCGCAA
GAAAGTTGCTCAAGCACGGGCTCGAGGCTGAACGAAAAGTGGTGGGTGTCGACGGCGCATCAGTGCCGGTGCGGATTATT
CGGCCAAAGACAAAGGCCAAAGGTGTGGTGCTGGATATTCATGGTGGCGGCTGGGTCATCGGCAACGCGCAGATGAACGA
CCCCCTCAATATCGCCATGGTGAAAGCCTGCGACGTGGCAGTGGTCTCGGTCGACTATCGGCTTGTGGTCTCGACTCCGA
TCGAGGGCGTCATGCAGGACTGCCTCACGGCGGCCCGCTGGCTGCTGAGCGATGACTGCCGCGAGTTTGCGAACCTGCCC
GTCATCGTCGTGGGCGAGTCGGCAGGCGGCCATCTCGCCGTCGCGACCCTGTTGCAACTCAAACAGTGGCCGGACCTGCT
CGAACGGGTGAGCGGGGCGCTGCTGTATTACGGCGTCTATGACCTTGCCGGAACCCCCAGCGTACGCAACGCCGGGCCCG
AGACCTTGCTGCTGGACGGGCCAGGGATGGTTGAAGCGCTGCAAATGCTCACGCCGGGATTGAACGATGAAGAACGCCGG
CAGGCGCCGTTGTCACCGCTGTACGGTGACTTCACCGGTTTTCCGCCAGCGTTGATGTTTGCTGCGCAACTGGATCCGCT
CAGGGATGACACGCTTGAGTTGGCTGAGCGTTGGGGCAGGGCGGCGGAAGTGGAGGTGCATCTGTTGCCGGAAACCGCAC
ATGGGGTGATTCATTTTCCGCTGGGGTTGGCGGGGAGGGTGCTTGGGTATAGTCGTACGTGGGTTGCGGGGCGGCTTGTT
GAGGGGTGA

Upstream 100 bases:

>100_bases
CAATGAGCTTTTTGCGTTGCTCCGGGTATCCAGGCAGCAACAGACCCAATGCGCTGATATTTTTTCATTCTGCGCAACGC
TCGCGAATAAAGAGGACGAA

Downstream 100 bases:

>100_bases
GCCATCGACCCTCACTCAGCCGCCACCTTGAATTGCTCGAGAATAAGTTCAGCCAATGTCCTGACAGGCTCGGTGAAACT
TCGCCGAGGCCTGTGCACCA

Product: esterase/lipase/thioesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKKSMDWPADANELAQLRAFNRKLARLPRFRIRNRITPRAIQLLLRLSQLGGARKLLKHGLEAERKVVGVDGASVPVRII
RPKTKAKGVVLDIHGGGWVIGNAQMNDPLNIAMVKACDVAVVSVDYRLVVSTPIEGVMQDCLTAARWLLSDDCREFANLP
VIVVGESAGGHLAVATLLQLKQWPDLLERVSGALLYYGVYDLAGTPSVRNAGPETLLLDGPGMVEALQMLTPGLNDEERR
QAPLSPLYGDFTGFPPALMFAAQLDPLRDDTLELAERWGRAAEVEVHLLPETAHGVIHFPLGLAGRVLGYSRTWVAGRLV
EG

Sequences:

>Translated_322_residues
MKKSMDWPADANELAQLRAFNRKLARLPRFRIRNRITPRAIQLLLRLSQLGGARKLLKHGLEAERKVVGVDGASVPVRII
RPKTKAKGVVLDIHGGGWVIGNAQMNDPLNIAMVKACDVAVVSVDYRLVVSTPIEGVMQDCLTAARWLLSDDCREFANLP
VIVVGESAGGHLAVATLLQLKQWPDLLERVSGALLYYGVYDLAGTPSVRNAGPETLLLDGPGMVEALQMLTPGLNDEERR
QAPLSPLYGDFTGFPPALMFAAQLDPLRDDTLELAERWGRAAEVEVHLLPETAHGVIHFPLGLAGRVLGYSRTWVAGRLV
EG
>Mature_322_residues
MKKSMDWPADANELAQLRAFNRKLARLPRFRIRNRITPRAIQLLLRLSQLGGARKLLKHGLEAERKVVGVDGASVPVRII
RPKTKAKGVVLDIHGGGWVIGNAQMNDPLNIAMVKACDVAVVSVDYRLVVSTPIEGVMQDCLTAARWLLSDDCREFANLP
VIVVGESAGGHLAVATLLQLKQWPDLLERVSGALLYYGVYDLAGTPSVRNAGPETLLLDGPGMVEALQMLTPGLNDEERR
QAPLSPLYGDFTGFPPALMFAAQLDPLRDDTLELAERWGRAAEVEVHLLPETAHGVIHFPLGLAGRVLGYSRTWVAGRLV
EG

Specific function: Lactone hydrolase that is required for the degradation of monocyclic monoterpenes such as menthol and carvone. Catalyzes the ring opening of lactones formed during degradation of monocyclic monoterpenes. Can hydrolyze epsilon-caprolactone, the lactone der

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=238, Percent_Identity=26.4705882352941, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=120, Percent_Identity=40, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17540028, Length=174, Percent_Identity=29.3103448275862, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71996133, Length=174, Percent_Identity=28.1609195402299, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.83 [H]

Molecular weight: Translated: 35151; Mature: 35151

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSMDWPADANELAQLRAFNRKLARLPRFRIRNRITPRAIQLLLRLSQLGGARKLLKHG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCC
LEAERKVVGVDGASVPVRIIRPKTKAKGVVLDIHGGGWVIGNAQMNDPLNIAMVKACDVA
CCHHCEEEECCCCCCCEEEECCCCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEECCEE
VVSVDYRLVVSTPIEGVMQDCLTAARWLLSDDCREFANLPVIVVGESAGGHLAVATLLQL
EEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
KQWPDLLERVSGALLYYGVYDLAGTPSVRNAGPETLLLDGPGMVEALQMLTPGLNDEERR
HHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCHHHH
QAPLSPLYGDFTGFPPALMFAAQLDPLRDDTLELAERWGRAAEVEVHLLPETAHGVIHFP
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEC
LGLAGRVLGYSRTWVAGRLVEG
CHHHHHHHCCCCHHHCCEECCC
>Mature Secondary Structure
MKKSMDWPADANELAQLRAFNRKLARLPRFRIRNRITPRAIQLLLRLSQLGGARKLLKHG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHCC
LEAERKVVGVDGASVPVRIIRPKTKAKGVVLDIHGGGWVIGNAQMNDPLNIAMVKACDVA
CCHHCEEEECCCCCCCEEEECCCCCCCEEEEEECCCCEEEECCCCCCCCCEEEEEECCEE
VVSVDYRLVVSTPIEGVMQDCLTAARWLLSDDCREFANLPVIVVGESAGGHLAVATLLQL
EEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHH
KQWPDLLERVSGALLYYGVYDLAGTPSVRNAGPETLLLDGPGMVEALQMLTPGLNDEERR
HHHHHHHHHHCCCEEEEEHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCHHHH
QAPLSPLYGDFTGFPPALMFAAQLDPLRDDTLELAERWGRAAEVEVHLLPETAHGVIHFP
CCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCEEEEC
LGLAGRVLGYSRTWVAGRLVEG
CHHHHHHHCCCCHHHCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA