| Definition | Pseudomonas fluorescens Pf0-1 chromosome, complete genome. |
|---|---|
| Accession | NC_007492 |
| Length | 6,438,405 |
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The map label for this gene is yusZ [H]
Identifier: 77458465
GI number: 77458465
Start: 2568586
End: 2569113
Strand: Reverse
Name: yusZ [H]
Synonym: Pfl01_2238
Alternate gene names: 77458465
Gene position: 2569113-2568586 (Counterclockwise)
Preceding gene: 77458467
Following gene: 77458463
Centisome position: 39.9
GC content: 63.26
Gene sequence:
>528_bases ATGACCACCACCAAAACCCTCTTCATCACCGGCGTCAGCAGCGGCTTCGGCCACGCGCTGGCCAAGGAAGCGCTGGCTGC CGGTCACCGCGTCATCGGCACCGTGCGCAGCGGAGCCGACCTGCAAGCCTTTCAGGCGCTGTCCATCGACAACGCGTACG GCGTGCTGCTGGACGTCACCGACTTCGACCGCATCGAAGGCGTCGTGGCGACTGTCGAAGCGACCCACGGCCCGGTCGAT GTGCTGGTCAACAACGCCGGCTACGGCCACGAAGGCATTTTAGAAGAATCGCCGCTGGAGGACATGCGCCGCCAGTTCGA CGTGAATGTGTTCGGCGCGGTCGCGGTGACCAAGGCGTTTGTGCCGTTCTTTCGCCAGCGGCGGGCGGGTCATATTCTCA ATATCACCTCGATGGGCGGCACAATCACCATGCCGGGTATCGCGTACTACTGTGGCAGCAAGTTTGCGCTCGAAGGCGTT GCGCCATGCCATGACTGCCTCAGCGGCGGCGTTCAGTGTCGGCTATGA
Upstream 100 bases:
>100_bases GCCGTTGATAGGAATAGGCAATGATCAAAGGCAAACCGACATAGGTGAGTGTTTCTTCGATGGATACCATGAGTTGAAAC CGCATTGACTGGAGCCCGTC
Downstream 100 bases:
>100_bases AAGCGCTTCGCAATTCAGCCGCGAGTTCAAAAGGTTCTTTGGCAGGACACCGCTGGCGGAGATCGAGTGGATGAAGGCAA CGTATGCGTTGCCTGCGCCG
Product: Short-chain dehydrogenase/reductase SDR
Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]
Alternate protein names: ORFA [H]
Number of amino acids: Translated: 175; Mature: 174
Protein sequence:
>175_residues MTTTKTLFITGVSSGFGHALAKEALAAGHRVIGTVRSGADLQAFQALSIDNAYGVLLDVTDFDRIEGVVATVEATHGPVD VLVNNAGYGHEGILEESPLEDMRRQFDVNVFGAVAVTKAFVPFFRQRRAGHILNITSMGGTITMPGIAYYCGSKFALEGV APCHDCLSGGVQCRL
Sequences:
>Translated_175_residues MTTTKTLFITGVSSGFGHALAKEALAAGHRVIGTVRSGADLQAFQALSIDNAYGVLLDVTDFDRIEGVVATVEATHGPVD VLVNNAGYGHEGILEESPLEDMRRQFDVNVFGAVAVTKAFVPFFRQRRAGHILNITSMGGTITMPGIAYYCGSKFALEGV APCHDCLSGGVQCRL >Mature_174_residues TTTKTLFITGVSSGFGHALAKEALAAGHRVIGTVRSGADLQAFQALSIDNAYGVLLDVTDFDRIEGVVATVEATHGPVDV LVNNAGYGHEGILEESPLEDMRRQFDVNVFGAVAVTKAFVPFFRQRRAGHILNITSMGGTITMPGIAYYCGSKFALEGVA PCHDCLSGGVQCRL
Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]
COG id: COG1028
COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]
Homologues:
Organism=Homo sapiens, GI20149619, Length=173, Percent_Identity=34.1040462427746, Blast_Score=91, Evalue=4e-19, Organism=Homo sapiens, GI7657478, Length=163, Percent_Identity=38.6503067484663, Blast_Score=89, Evalue=3e-18, Organism=Homo sapiens, GI7706318, Length=164, Percent_Identity=29.8780487804878, Blast_Score=82, Evalue=3e-16, Organism=Homo sapiens, GI4503817, Length=163, Percent_Identity=31.9018404907975, Blast_Score=80, Evalue=1e-15, Organism=Homo sapiens, GI40548397, Length=162, Percent_Identity=33.9506172839506, Blast_Score=78, Evalue=5e-15, Organism=Homo sapiens, GI214010158, Length=162, Percent_Identity=33.9506172839506, Blast_Score=78, Evalue=5e-15, Organism=Homo sapiens, GI214010156, Length=162, Percent_Identity=33.9506172839506, Blast_Score=78, Evalue=5e-15, Organism=Homo sapiens, GI22758144, Length=162, Percent_Identity=33.9506172839506, Blast_Score=78, Evalue=5e-15, Organism=Homo sapiens, GI223718074, Length=163, Percent_Identity=36.8098159509202, Blast_Score=74, Evalue=5e-14, Organism=Homo sapiens, GI44680136, Length=168, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-13, Organism=Homo sapiens, GI44680133, Length=168, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-13, Organism=Homo sapiens, GI17738292, Length=168, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=1e-13, Organism=Homo sapiens, GI22507405, Length=162, Percent_Identity=32.7160493827161, Blast_Score=70, Evalue=1e-12, Organism=Homo sapiens, GI150247226, Length=167, Percent_Identity=32.3353293413174, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI19743808, Length=171, Percent_Identity=32.1637426900585, Blast_Score=69, Evalue=3e-12, Organism=Homo sapiens, GI157739938, Length=177, Percent_Identity=29.3785310734463, Blast_Score=68, Evalue=4e-12, Organism=Homo sapiens, GI50726952, Length=160, Percent_Identity=34.375, Blast_Score=67, Evalue=6e-12, Organism=Homo sapiens, GI210032110, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11, Organism=Homo sapiens, GI142976729, Length=104, Percent_Identity=33.6538461538462, Blast_Score=66, Evalue=2e-11, Organism=Homo sapiens, GI109715829, Length=159, Percent_Identity=27.0440251572327, Blast_Score=65, Evalue=2e-11, Organism=Escherichia coli, GI1787335, Length=159, Percent_Identity=33.9622641509434, Blast_Score=84, Evalue=7e-18, Organism=Escherichia coli, GI1787820, Length=150, Percent_Identity=35.3333333333333, Blast_Score=82, Evalue=1e-17, Organism=Escherichia coli, GI1786701, Length=155, Percent_Identity=32.258064516129, Blast_Score=69, Evalue=2e-13, Organism=Caenorhabditis elegans, GI193203115, Length=155, Percent_Identity=32.9032258064516, Blast_Score=72, Evalue=1e-13, Organism=Caenorhabditis elegans, GI193203117, Length=155, Percent_Identity=32.9032258064516, Blast_Score=72, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17564282, Length=167, Percent_Identity=29.3413173652695, Blast_Score=70, Evalue=8e-13, Organism=Caenorhabditis elegans, GI115534694, Length=162, Percent_Identity=29.6296296296296, Blast_Score=67, Evalue=3e-12, Organism=Caenorhabditis elegans, GI17570069, Length=166, Percent_Identity=30.7228915662651, Blast_Score=67, Evalue=4e-12, Organism=Caenorhabditis elegans, GI17567345, Length=160, Percent_Identity=30, Blast_Score=67, Evalue=5e-12, Organism=Caenorhabditis elegans, GI71997402, Length=171, Percent_Identity=30.9941520467836, Blast_Score=65, Evalue=1e-11, Organism=Caenorhabditis elegans, GI17508895, Length=169, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=2e-11, Organism=Caenorhabditis elegans, GI17538486, Length=183, Percent_Identity=27.8688524590164, Blast_Score=63, Evalue=6e-11, Organism=Saccharomyces cerevisiae, GI6323882, Length=163, Percent_Identity=31.2883435582822, Blast_Score=81, Evalue=1e-16, Organism=Saccharomyces cerevisiae, GI6320089, Length=171, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=5e-14, Organism=Drosophila melanogaster, GI21358495, Length=164, Percent_Identity=35.3658536585366, Blast_Score=89, Evalue=1e-18, Organism=Drosophila melanogaster, GI281364892, Length=160, Percent_Identity=27.5, Blast_Score=79, Evalue=1e-15, Organism=Drosophila melanogaster, GI24580925, Length=166, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=4e-13, Organism=Drosophila melanogaster, GI24641388, Length=166, Percent_Identity=34.9397590361446, Blast_Score=70, Evalue=7e-13, Organism=Drosophila melanogaster, GI24640492, Length=158, Percent_Identity=27.2151898734177, Blast_Score=68, Evalue=3e-12, Organism=Drosophila melanogaster, GI24643142, Length=156, Percent_Identity=29.4871794871795, Blast_Score=67, Evalue=7e-12, Organism=Drosophila melanogaster, GI21357041, Length=165, Percent_Identity=30.9090909090909, Blast_Score=66, Evalue=1e-11, Organism=Drosophila melanogaster, GI21355631, Length=161, Percent_Identity=26.7080745341615, Blast_Score=64, Evalue=4e-11, Organism=Drosophila melanogaster, GI28571387, Length=163, Percent_Identity=30.6748466257669, Blast_Score=63, Evalue=9e-11, Organism=Drosophila melanogaster, GI28571526, Length=163, Percent_Identity=30.0613496932515, Blast_Score=63, Evalue=1e-10,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR002198 - InterPro: IPR002347 - InterPro: IPR016040 - InterPro: IPR020904 [H]
Pfam domain/function: PF00106 adh_short [H]
EC number: 1.1.1.100 [C]
Molecular weight: Translated: 18533; Mature: 18402
Theoretical pI: Translated: 6.22; Mature: 6.22
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.3 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 4.6 %Cys+Met (Translated Protein) 2.3 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 4.0 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTTKTLFITGVSSGFGHALAKEALAAGHRVIGTVRSGADLQAFQALSIDNAYGVLLDVT CCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEECCCCCCEEEEECC DFDRIEGVVATVEATHGPVDVLVNNAGYGHEGILEESPLEDMRRQFDVNVFGAVAVTKAF CCHHHCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH VPFFRQRRAGHILNITSMGGTITMPGIAYYCGSKFALEGVAPCHDCLSGGVQCRL HHHHHHHCCCCEEEEECCCCEEECCCCHHHHCCCEEECCCCHHHHHHCCCEEECC >Mature Secondary Structure TTTKTLFITGVSSGFGHALAKEALAAGHRVIGTVRSGADLQAFQALSIDNAYGVLLDVT CCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHEECCCCCCEEEEECC DFDRIEGVVATVEATHGPVDVLVNNAGYGHEGILEESPLEDMRRQFDVNVFGAVAVTKAF CCHHHCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH VPFFRQRRAGHILNITSMGGTITMPGIAYYCGSKFALEGVAPCHDCLSGGVQCRL HHHHHHHCCCCEEEEECCCCEEECCCCHHHHCCCEEECCCCHHHHHHCCCEEECC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NADPH [C]
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]
Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]
General reaction: Redox reaction [C]
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 9353931; 9384377; 8396117 [H]