Click here to switch to the map view.

The map label for this gene is puuB [H]

Identifier: 77458369

GI number: 77458369

Start: 2425466

End: 2426749

Strand: Reverse

Name: puuB [H]

Synonym: Pfl01_2142

Alternate gene names: 77458369

Gene position: 2426749-2425466 (Counterclockwise)

Preceding gene: 77458370

Following gene: 77458366

Centisome position: 37.69

GC content: 62.38

Gene sequence:

>1284_bases
ATGACTGAACGCAGCAATTCCTACTACACCGCGACCCTCAACCGCGACACTGACTACCCGACCCTGCAAGGTCGGCACAA
GGTCGATGTGGTGATCATCGGTGGCGGTTTCACCGGCGTCGCCACCGCCGTCGAGCTGGCCGAGAAAGGCCTGAAAGTCG
CCATCGTCGAAAGCCACAAGATCGGCTGGGGCGCCACCGGGCGCAATGGCGGGCAGGTCACCGGCAGCCTGTCCGGCGAC
GGCGCGATGCGCAAACAAATGCGCAACACCTTGGGCGACGAGGTCGATGACTTTATCTGGCACCTGCGCTGGCGTGGGCA
CGAAATCATCAAACAGCGCGTCGAGAAATACGCGATCGATTGCGACCTCAAGCACGGCCATTTGCACGCCGCCTACAAGC
CGAGCCACATGGCCGGTTTGCGCAGCGATTACGACGAAGCCGTGCGTCGCGGCATGGGTGATGAAGTCAGCCTGCTCGAT
CGCAGCCAGGTGCGCGACCTGCTGCAAAGCGAGCTCTATCACGGCGCAATCAAAAACACTCGCAACATGCACCTGCACCC
GCTCAACCTGTGCATCGGTGAAGCGCGAGCGGCGGAAAGCAACGGCGCGCTGATCTTCGAAAACAGCGAAGTGCTGGAGA
TCATTCACGGCGACAGCCCCGGCGTGCGCACCACCCACGGCCGGATCGACGCCAATCAGGTGCTGCTGGCCGGCGACGTC
TACCACAAGCTCGAGCCGGGCCAGCTCAAGGGCAAGATCTTCCCGGCCATGGGCGGCATCGTCACCACCGAACCATTGGG
CGATCTGGCCAGACAGATCAACCCTGAAGACCTCGCCGTTTACGACTGCCGCTTCGTCCTCGATTACTACCGCCTCACCG
CCGACGGTCGCCTGCTGTTCGGCGGCGGCGCCAACTACAGCGGCAAGGACTCACGGGACATCGCCGGCGAACTGCGGCCG
TGCATCGAACAGACTTTCCCGGCGCTCAAAGGGGTGAAGATCGATTACCAGTGGAGCTGCGCGATGGGCATCGTCATCAA
CCGCATCCCGCAACTGGGCAAGCTCTCGGACAACGTCTGGTATTGCCAGGGCTACTCCGGCCACGGCATCGCTACGACCC
ACATCATGGGTGAAATCATGAGCCGGGCGATCACCGGGCAGATGCAGCAGTTCGACACGTTTGCCGCGTGCTCGCACATT
CGCGTGCCGATGGGCGATCTGCTGGGGAATCCGATGCTGGCGGCCGGCATGTGGTACTACCAGATGCTGGAAAAACTGCG
CTGA

Upstream 100 bases:

>100_bases
CGGAATACCGGCAGTTCATGGCTGAAGTGGGTGAGCAGGACTGGCGCTGGTATCTGACCGAAGCCTGATCCTTTTTCCTC
TTCTTGCCCTGGATACCGAC

Downstream 100 bases:

>100_bases
TCGAACAGGCCCCATCGCCAGTGATGGGGTCTTTTTTGCCGCAAAACCTCAGTCGGGCAACTGCTCCACCTCAATCCCCA
ACCGCCGATAATCCTCGACA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTERSNSYYTATLNRDTDYPTLQGRHKVDVVIIGGGFTGVATAVELAEKGLKVAIVESHKIGWGATGRNGGQVTGSLSGD
GAMRKQMRNTLGDEVDDFIWHLRWRGHEIIKQRVEKYAIDCDLKHGHLHAAYKPSHMAGLRSDYDEAVRRGMGDEVSLLD
RSQVRDLLQSELYHGAIKNTRNMHLHPLNLCIGEARAAESNGALIFENSEVLEIIHGDSPGVRTTHGRIDANQVLLAGDV
YHKLEPGQLKGKIFPAMGGIVTTEPLGDLARQINPEDLAVYDCRFVLDYYRLTADGRLLFGGGANYSGKDSRDIAGELRP
CIEQTFPALKGVKIDYQWSCAMGIVINRIPQLGKLSDNVWYCQGYSGHGIATTHIMGEIMSRAITGQMQQFDTFAACSHI
RVPMGDLLGNPMLAAGMWYYQMLEKLR

Sequences:

>Translated_427_residues
MTERSNSYYTATLNRDTDYPTLQGRHKVDVVIIGGGFTGVATAVELAEKGLKVAIVESHKIGWGATGRNGGQVTGSLSGD
GAMRKQMRNTLGDEVDDFIWHLRWRGHEIIKQRVEKYAIDCDLKHGHLHAAYKPSHMAGLRSDYDEAVRRGMGDEVSLLD
RSQVRDLLQSELYHGAIKNTRNMHLHPLNLCIGEARAAESNGALIFENSEVLEIIHGDSPGVRTTHGRIDANQVLLAGDV
YHKLEPGQLKGKIFPAMGGIVTTEPLGDLARQINPEDLAVYDCRFVLDYYRLTADGRLLFGGGANYSGKDSRDIAGELRP
CIEQTFPALKGVKIDYQWSCAMGIVINRIPQLGKLSDNVWYCQGYSGHGIATTHIMGEIMSRAITGQMQQFDTFAACSHI
RVPMGDLLGNPMLAAGMWYYQMLEKLR
>Mature_426_residues
TERSNSYYTATLNRDTDYPTLQGRHKVDVVIIGGGFTGVATAVELAEKGLKVAIVESHKIGWGATGRNGGQVTGSLSGDG
AMRKQMRNTLGDEVDDFIWHLRWRGHEIIKQRVEKYAIDCDLKHGHLHAAYKPSHMAGLRSDYDEAVRRGMGDEVSLLDR
SQVRDLLQSELYHGAIKNTRNMHLHPLNLCIGEARAAESNGALIFENSEVLEIIHGDSPGVRTTHGRIDANQVLLAGDVY
HKLEPGQLKGKIFPAMGGIVTTEPLGDLARQINPEDLAVYDCRFVLDYYRLTADGRLLFGGGANYSGKDSRDIAGELRPC
IEQTFPALKGVKIDYQWSCAMGIVINRIPQLGKLSDNVWYCQGYSGHGIATTHIMGEIMSRAITGQMQQFDTFAACSHIR
VPMGDLLGNPMLAAGMWYYQMLEKLR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=429, Percent_Identity=39.6270396270396, Blast_Score=323, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47232; Mature: 47100

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERSNSYYTATLNRDTDYPTLQGRHKVDVVIIGGGFTGVATAVELAEKGLKVAIVESHK
CCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
IGWGATGRNGGQVTGSLSGDGAMRKQMRNTLGDEVDDFIWHLRWRGHEIIKQRVEKYAID
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHEEC
CDLKHGHLHAAYKPSHMAGLRSDYDEAVRRGMGDEVSLLDRSQVRDLLQSELYHGAIKNT
EECCCCEEEEEECCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RNMHLHPLNLCIGEARAAESNGALIFENSEVLEIIHGDSPGVRTTHGRIDANQVLLAGDV
CCCEEEEHHHHHCCHHCCCCCCEEEEECCCEEEEEECCCCCCEEECCCCCCCCEEEECCH
YHKLEPGQLKGKIFPAMGGIVTTEPLGDLARQINPEDLAVYDCRFVLDYYRLTADGRLLF
HHCCCCCCCCCEEECCCCCEEECCCHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEEE
GGGANYSGKDSRDIAGELRPCIEQTFPALKGVKIDYQWSCAMGIVINRIPQLGKLSDNVW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCCCCCCCCEE
YCQGYSGHGIATTHIMGEIMSRAITGQMQQFDTFAACSHIRVPMGDLLGNPMLAAGMWYY
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHH
QMLEKLR
HHHHHCC
>Mature Secondary Structure 
TERSNSYYTATLNRDTDYPTLQGRHKVDVVIIGGGFTGVATAVELAEKGLKVAIVESHK
CCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCC
IGWGATGRNGGQVTGSLSGDGAMRKQMRNTLGDEVDDFIWHLRWRGHEIIKQRVEKYAID
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHEEC
CDLKHGHLHAAYKPSHMAGLRSDYDEAVRRGMGDEVSLLDRSQVRDLLQSELYHGAIKNT
EECCCCEEEEEECCHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RNMHLHPLNLCIGEARAAESNGALIFENSEVLEIIHGDSPGVRTTHGRIDANQVLLAGDV
CCCEEEEHHHHHCCHHCCCCCCEEEEECCCEEEEEECCCCCCEEECCCCCCCCEEEECCH
YHKLEPGQLKGKIFPAMGGIVTTEPLGDLARQINPEDLAVYDCRFVLDYYRLTADGRLLF
HHCCCCCCCCCEEECCCCCEEECCCHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEEE
GGGANYSGKDSRDIAGELRPCIEQTFPALKGVKIDYQWSCAMGIVINRIPQLGKLSDNVW
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHCCCCCCCCCCCEE
YCQGYSGHGIATTHIMGEIMSRAITGQMQQFDTFAACSHIRVPMGDLLGNPMLAAGMWYY
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHH
QMLEKLR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]