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The map label for this gene is puuA [H]

Identifier: 77458370

GI number: 77458370

Start: 2426782

End: 2428146

Strand: Reverse

Name: puuA [H]

Synonym: Pfl01_2143

Alternate gene names: 77458370

Gene position: 2428146-2426782 (Counterclockwise)

Preceding gene: 77458371

Following gene: 77458369

Centisome position: 37.71

GC content: 63.81

Gene sequence:

>1365_bases
ATGAAATTCGCAGCCATTGAAGACGCCCGCAGCTTTCTCGAGCACAACCCCGACATCGACATGATCGAGCTGTTCATCCT
CGACGCCAACGGCGTGCCCCGCGGCAAGCTGTTGCACCGCGAAGAACTGCTCGCCGTATACGAAAGTGGCCGCCCGCTGC
CAAGCACCATCCTCGGCCTGACGGTGCACGGCGAAGACGTGGAGAACTCCGGTCTGGTGTGGGACGTCGGCGACATCGAC
TGCCGCGCCTACCCGCTGGAAGGCAGTCTGGTACGCCTGCCGTGGCGGCAGATTCCGACTGCTGCGGTGCAGGTCAGCAT
GCATCCGGAACAAGGCATGCCGGCGAGCATTGCCGACCCGCGTCACCTGTTGATCAAGATCATCGACCGCTTGAAAGCCG
AGGGTTACCACCCGGTAATGGCCTGCGAGCTGGAGTTCTATCTGCTCGACGCCAAACGCGATCACAACGGTCGCCCGCAA
CCGGCGCTGGATGCCGACGGTGGTCGACCGAGACACACGCAGGTCTACGGTTTGCGTGAGCTGGAACAGATCGAACCGTT
CCTCGCCGATCTCTACAGCGCCTGCAAACTGCACGGCATTCCGGCGCGCACGGCGATCTCGGAATACGCGCCGGGCCAGG
TGGAAATTACCCTCGAACACGGCGATGCACTGGAGGCGATGGACCAGGCCGTGCGCTACAAACGACTGGTCAAAGCCGTG
GCGCACAAGCACGGGATGCAGGCGACGTTCATGGCCAAGCCGTTCGATCACCTGGCTGGCACCGGCATGCACATGCACGT
CAGCCTCGCCGATGCCGAGGGACGCAATCTGTTTGCCTCCGAGGACCCGGCCGGCACCCCGCTGTTGCGCACGGCGATTG
GCGGGATGCTCGCTTCCCTGCTCGATTCGCTGCTGCTGTTCTGCCCGAACGCCAACTCCTACCGTCGCTTCCAGGCCAAC
AGTTACGCGCCACTGGCGCCGACCTGGGGCGTCGACAACCGTACCGTCAGCCTGCGCGTGCCCGGCGGCCCGGCCAACAC
CCGGCACATCGAACACCGCATCTGCGGCGCCGATGCCAACCCGTATCTGGCAGCGGCGGCGATCCTGGCGGGCATTCATC
GCGGGATAAAAGAAGACATCGATCCGGGTGAACCGGTGGAAGGCAACGGTTACGCGCAGGCGAAAGAATTGCTGCCGACC
GACTGGCTGACCTCGCTTACGGCGCTGGAGAATTCGGTGTGGGCGCGGGATGCGCTGGGGCAGGAGTTTCTCGGGGTGTA
TCTGGCGGTGAAACGCGCGGAATACCGGCAGTTCATGGCTGAAGTGGGTGAGCAGGACTGGCGCTGGTATCTGACCGAAG
CCTGA

Upstream 100 bases:

>100_bases
CTCTTTTTTCGATATCCCTGCCAGGACGCCCGCGCCTTTTGCCGCGCTGCGTCCGGCCCGAATCTGCGCGCCTGTGCGGC
GCCATCGTGAGTGATTGATT

Downstream 100 bases:

>100_bases
TCCTTTTTCCTCTTCTTGCCCTGGATACCGACATGACTGAACGCAGCAATTCCTACTACACCGCGACCCTCAACCGCGAC
ACTGACTACCCGACCCTGCA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKFAAIEDARSFLEHNPDIDMIELFILDANGVPRGKLLHREELLAVYESGRPLPSTILGLTVHGEDVENSGLVWDVGDID
CRAYPLEGSLVRLPWRQIPTAAVQVSMHPEQGMPASIADPRHLLIKIIDRLKAEGYHPVMACELEFYLLDAKRDHNGRPQ
PALDADGGRPRHTQVYGLRELEQIEPFLADLYSACKLHGIPARTAISEYAPGQVEITLEHGDALEAMDQAVRYKRLVKAV
AHKHGMQATFMAKPFDHLAGTGMHMHVSLADAEGRNLFASEDPAGTPLLRTAIGGMLASLLDSLLLFCPNANSYRRFQAN
SYAPLAPTWGVDNRTVSLRVPGGPANTRHIEHRICGADANPYLAAAAILAGIHRGIKEDIDPGEPVEGNGYAQAKELLPT
DWLTSLTALENSVWARDALGQEFLGVYLAVKRAEYRQFMAEVGEQDWRWYLTEA

Sequences:

>Translated_454_residues
MKFAAIEDARSFLEHNPDIDMIELFILDANGVPRGKLLHREELLAVYESGRPLPSTILGLTVHGEDVENSGLVWDVGDID
CRAYPLEGSLVRLPWRQIPTAAVQVSMHPEQGMPASIADPRHLLIKIIDRLKAEGYHPVMACELEFYLLDAKRDHNGRPQ
PALDADGGRPRHTQVYGLRELEQIEPFLADLYSACKLHGIPARTAISEYAPGQVEITLEHGDALEAMDQAVRYKRLVKAV
AHKHGMQATFMAKPFDHLAGTGMHMHVSLADAEGRNLFASEDPAGTPLLRTAIGGMLASLLDSLLLFCPNANSYRRFQAN
SYAPLAPTWGVDNRTVSLRVPGGPANTRHIEHRICGADANPYLAAAAILAGIHRGIKEDIDPGEPVEGNGYAQAKELLPT
DWLTSLTALENSVWARDALGQEFLGVYLAVKRAEYRQFMAEVGEQDWRWYLTEA
>Mature_454_residues
MKFAAIEDARSFLEHNPDIDMIELFILDANGVPRGKLLHREELLAVYESGRPLPSTILGLTVHGEDVENSGLVWDVGDID
CRAYPLEGSLVRLPWRQIPTAAVQVSMHPEQGMPASIADPRHLLIKIIDRLKAEGYHPVMACELEFYLLDAKRDHNGRPQ
PALDADGGRPRHTQVYGLRELEQIEPFLADLYSACKLHGIPARTAISEYAPGQVEITLEHGDALEAMDQAVRYKRLVKAV
AHKHGMQATFMAKPFDHLAGTGMHMHVSLADAEGRNLFASEDPAGTPLLRTAIGGMLASLLDSLLLFCPNANSYRRFQAN
SYAPLAPTWGVDNRTVSLRVPGGPANTRHIEHRICGADANPYLAAAAILAGIHRGIKEDIDPGEPVEGNGYAQAKELLPT
DWLTSLTALENSVWARDALGQEFLGVYLAVKRAEYRQFMAEVGEQDWRWYLTEA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=421, Percent_Identity=24.7030878859857, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081870, Length=461, Percent_Identity=37.9609544468547, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1790301, Length=234, Percent_Identity=33.7606837606838, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50139; Mature: 50139

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAAIEDARSFLEHNPDIDMIELFILDANGVPRGKLLHREELLAVYESGRPLPSTILGL
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
TVHGEDVENSGLVWDVGDIDCRAYPLEGSLVRLPWRQIPTAAVQVSMHPEQGMPASIADP
EEECCCCCCCCCEEECCCCCEEEECCCCCEEECCHHHCCCEEEEEEECCCCCCCCCCCCH
RHLLIKIIDRLKAEGYHPVMACELEFYLLDAKRDHNGRPQPALDADGGRPRHTQVYGLRE
HHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCHHH
LEQIEPFLADLYSACKLHGIPARTAISEYAPGQVEITLEHGDALEAMDQAVRYKRLVKAV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
AHKHGMQATFMAKPFDHLAGTGMHMHVSLADAEGRNLFASEDPAGTPLLRTAIGGMLASL
HHHCCCCEEEECCCHHHHCCCCEEEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHH
LDSLLLFCPNANSYRRFQANSYAPLAPTWGVDNRTVSLRVPGGPANTRHIEHRICGADAN
HHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHEECCCCC
PYLAAAAILAGIHRGIKEDIDPGEPVEGNGYAQAKELLPTDWLTSLTALENSVWARDALG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QEFLGVYLAVKRAEYRQFMAEVGEQDWRWYLTEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MKFAAIEDARSFLEHNPDIDMIELFILDANGVPRGKLLHREELLAVYESGRPLPSTILGL
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEE
TVHGEDVENSGLVWDVGDIDCRAYPLEGSLVRLPWRQIPTAAVQVSMHPEQGMPASIADP
EEECCCCCCCCCEEECCCCCEEEECCCCCEEECCHHHCCCEEEEEEECCCCCCCCCCCCH
RHLLIKIIDRLKAEGYHPVMACELEFYLLDAKRDHNGRPQPALDADGGRPRHTQVYGLRE
HHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEECCHHH
LEQIEPFLADLYSACKLHGIPARTAISEYAPGQVEITLEHGDALEAMDQAVRYKRLVKAV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHH
AHKHGMQATFMAKPFDHLAGTGMHMHVSLADAEGRNLFASEDPAGTPLLRTAIGGMLASL
HHHCCCCEEEECCCHHHHCCCCEEEEEEEECCCCCCEECCCCCCCCHHHHHHHHHHHHHH
LDSLLLFCPNANSYRRFQANSYAPLAPTWGVDNRTVSLRVPGGPANTRHIEHRICGADAN
HHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHEECCCCC
PYLAAAAILAGIHRGIKEDIDPGEPVEGNGYAQAKELLPTDWLTSLTALENSVWARDALG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QEFLGVYLAVKRAEYRQFMAEVGEQDWRWYLTEA
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]