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The map label for this gene is puuB [H]

Identifier: 77458136

GI number: 77458136

Start: 2182832

End: 2184115

Strand: Reverse

Name: puuB [H]

Synonym: Pfl01_1909

Alternate gene names: 77458136

Gene position: 2184115-2182832 (Counterclockwise)

Preceding gene: 77458139

Following gene: 77458115

Centisome position: 33.92

GC content: 61.92

Gene sequence:

>1284_bases
ATGGCGAACACCCCTTACCCAGAGTCTTATTACGCTGCGTCGGCCAACGCCGTACCACCACGCCCTGCCCTGCAGGATGA
CGTGGAGACGGATGTCTGTGTGATCGGCGCTGGTTATACGGGACTCTCCTCGGCCCTGTTCCTGCTGGAGAACGGTTTTC
GCGTGACCGTGCTGGAAGCCGCCAAGGTCGGTTTCGGCGCTTCGGGGCGCAATGGCGGGCAAATCGTCAACAGCTACAGC
CGTGACATCGATGTGATCGAACGCAGCGTCGGCCCGAAGCAGGCGCAACTGCTCGGGCAGATGGCGTTCGAAGGCGGCCG
GATCATTCGTGAGCGCGTCAGCAAGTACAACATTCAGTGCGACTTGAAGGACGGTGGCGTATTCGCCGCCCTCACCGCCA
AGCAAATGGGCCACCTGGAATCGCAGAAGCGCTTGTGGGAACGCTTCGGCCATACCCAACTGGAACTGCTCGATCAACGC
CGCATCCGCGAAGTGGTGGCTTGTGACCAATATGTCGGTGGCATGCTCGACATGAGCGGCGGCCACATCCACCCGCTTAA
CCTGGCGCTGGGCGAAGCCGCCGCTGTGGAATCCCTGGGCGGCACCATCCATGAACAGTCGCCGGCCGTGCGCATCGAAC
GCGGCGCCAACCCGGTCGTGCATACACCGCAGGGCAAGGTCCGAGCCAAATTCATCATCGTTGCCGGCAACGCCTACCTC
GGCAATCTGATGCCGGAACTGGCGGCCAAATCGATGCCGTGCGGCACTCAGGTCATCACCACCGAACCGCTCGGCGATGA
ACTGGCGAAATCCTTGCTGCCGCAGGACTACTGCGTAGAAGACTGCAATTACCTGCTCGACTACTACCGCCTCACCGGCG
ACAAACGCCTGATCTTCGGCGGCGGCGTGGTATATGGCGCGCGGGATCCGGCAAACATCGAAGCGATCATCCGCCCGAAA
ATGCTCAAGGCCTTCCCGCAGCTCAAGGACGTGAAAATCGACTACGCCTGGACCGGCAATTTCCTGCTGACCCTGTCGCG
CCTGCCTCAGGTCGGACGTCTGGGCGACAACATCTATTATTCTCAAGGCTGCAGTGGCCACGGCGTGACCTATACACACC
TGGCCGGCAAGGTACTGGCCGAGGCTCTGCGCGGACAAGCCGAGCGTTTCGATGCGTTTGCCGACCTGCCGCACTACCCG
TTCCCCGGCGGGCAACTGCTGCGCACACCGTTTGCCGCACTGGGCGCGTGGTACTACGGACTGCGCGACAAGCTCGGTTT
CTGA

Upstream 100 bases:

>100_bases
TTTTGACCCGGCGCAAGAAGATTTCTCGGTGGCTGGCGGAGGTTTTTCAACTGGGGTAGGATCGCTGCACTCGTAAAATA
TATTAAACAGAGGCTGCAGG

Downstream 100 bases:

>100_bases
TGAGCAAAAAAAGGGCCACTGAAAAGTGGCCCTTTTTACATCCAGCATCAATCACAACTGATCCCGTGCAATCCCGCTGC
GAATCCGCAGGATATCTGCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MANTPYPESYYAASANAVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFRVTVLEAAKVGFGASGRNGGQIVNSYS
RDIDVIERSVGPKQAQLLGQMAFEGGRIIRERVSKYNIQCDLKDGGVFAALTAKQMGHLESQKRLWERFGHTQLELLDQR
RIREVVACDQYVGGMLDMSGGHIHPLNLALGEAAAVESLGGTIHEQSPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYL
GNLMPELAAKSMPCGTQVITTEPLGDELAKSLLPQDYCVEDCNYLLDYYRLTGDKRLIFGGGVVYGARDPANIEAIIRPK
MLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYP
FPGGQLLRTPFAALGAWYYGLRDKLGF

Sequences:

>Translated_427_residues
MANTPYPESYYAASANAVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFRVTVLEAAKVGFGASGRNGGQIVNSYS
RDIDVIERSVGPKQAQLLGQMAFEGGRIIRERVSKYNIQCDLKDGGVFAALTAKQMGHLESQKRLWERFGHTQLELLDQR
RIREVVACDQYVGGMLDMSGGHIHPLNLALGEAAAVESLGGTIHEQSPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYL
GNLMPELAAKSMPCGTQVITTEPLGDELAKSLLPQDYCVEDCNYLLDYYRLTGDKRLIFGGGVVYGARDPANIEAIIRPK
MLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYP
FPGGQLLRTPFAALGAWYYGLRDKLGF
>Mature_426_residues
ANTPYPESYYAASANAVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFRVTVLEAAKVGFGASGRNGGQIVNSYSR
DIDVIERSVGPKQAQLLGQMAFEGGRIIRERVSKYNIQCDLKDGGVFAALTAKQMGHLESQKRLWERFGHTQLELLDQRR
IREVVACDQYVGGMLDMSGGHIHPLNLALGEAAAVESLGGTIHEQSPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYLG
NLMPELAAKSMPCGTQVITTEPLGDELAKSLLPQDYCVEDCNYLLDYYRLTGDKRLIFGGGVVYGARDPANIEAIIRPKM
LKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYYSQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPF
PGGQLLRTPFAALGAWYYGLRDKLGF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=65.3301886792453, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46501; Mature: 46370

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANTPYPESYYAASANAVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFRVTVLEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
AKVGFGASGRNGGQIVNSYSRDIDVIERSVGPKQAQLLGQMAFEGGRIIRERVSKYNIQC
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEEE
DLKDGGVFAALTAKQMGHLESQKRLWERFGHTQLELLDQRRIREVVACDQYVGGMLDMSG
EECCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEECCC
GHIHPLNLALGEAAAVESLGGTIHEQSPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYL
CEEEEEHHHHCCHHHHHHHCCCCCCCCCCEEEECCCCCCEECCCCCEEEEEEEEECCHHH
GNLMPELAAKSMPCGTQVITTEPLGDELAKSLLPQDYCVEDCNYLLDYYRLTGDKRLIFG
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
GGVVYGARDPANIEAIIRPKMLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY
CEEEECCCCCCCCCEEECHHHHHHCCCHHCEEEEEEECCCCEEEEHHCCCCCCCCCCEEE
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAALGAWYYG
ECCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LRDKLGF
HHHHCCC
>Mature Secondary Structure 
ANTPYPESYYAASANAVPPRPALQDDVETDVCVIGAGYTGLSSALFLLENGFRVTVLEA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEEE
AKVGFGASGRNGGQIVNSYSRDIDVIERSVGPKQAQLLGQMAFEGGRIIRERVSKYNIQC
ECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCEEE
DLKDGGVFAALTAKQMGHLESQKRLWERFGHTQLELLDQRRIREVVACDQYVGGMLDMSG
EECCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEECCC
GHIHPLNLALGEAAAVESLGGTIHEQSPAVRIERGANPVVHTPQGKVRAKFIIVAGNAYL
CEEEEEHHHHCCHHHHHHHCCCCCCCCCCEEEECCCCCCEECCCCCEEEEEEEEECCHHH
GNLMPELAAKSMPCGTQVITTEPLGDELAKSLLPQDYCVEDCNYLLDYYRLTGDKRLIFG
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEE
GGVVYGARDPANIEAIIRPKMLKAFPQLKDVKIDYAWTGNFLLTLSRLPQVGRLGDNIYY
CEEEECCCCCCCCCEEECHHHHHHCCCHHCEEEEEEECCCCEEEEHHCCCCCCCCCCEEE
SQGCSGHGVTYTHLAGKVLAEALRGQAERFDAFADLPHYPFPGGQLLRTPFAALGAWYYG
ECCCCCCCEEHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
LRDKLGF
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]