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The map label for this gene is metZ [H]

Identifier: 77458130

GI number: 77458130

Start: 2174331

End: 2175542

Strand: Direct

Name: metZ [H]

Synonym: Pfl01_1903

Alternate gene names: 77458130

Gene position: 2174331-2175542 (Clockwise)

Preceding gene: 77458129

Following gene: 77458131

Centisome position: 33.77

GC content: 62.62

Gene sequence:

>1212_bases
ATGAGTCAGGAATGGGATGCCGGTCGGCTGGACAGCGACCTCGAAGGCGTAGCGTTCGATACCCTGGCCGTACGTGCCGG
TCAGCACCGTACGCCGGAAGGCGAGCACGGTGATCCGATGTTCTTCACTTCCAGCTATGTGTTTCGCACCGCCGCCGATG
CGGCTGCGCGGTTTGCCGGGGAAGTGCCGGGCAACGTCTACTCGCGTTACACCAACCCGACCGTTCGCGCCTTCGAAGAG
CGCATTGCCGCGCTGGAAAGCGCCGAGCAGGCGGTGGCCACGGCCACCGGCATGGCCGCGATCATGGCTGTGGTGATGAG
CCTGTGCAGCGCCGGCGACCATGTGCTGGTGTCGCGCAGTGTGTTCGGTTCGACCATCAGCCTGTTCGAGAAGTATTTCA
AGCGTTTTGGCGTGGAAGTCGATTACGTGCCGCTGGCCGATCTGTCGGCCTGGGGCGCAGCGATCAAGTCCAACACCAAA
TTGCTGTTCGTCGAGTCGCCGTCCAATCCGTTGGCTGAACTGGTGGATATCACCGCTCTGTCGGAAATCGCGCACGCCAA
GGGTGCGATGCTGGTAGTCGACAACTGCTTCTGCACGCCTGCCTTGCAGCAGCCGCTGAAGCTGGGCGCAGACATCGTTG
TGCATTCGGCCACCAAGTTCATCGATGGCCAGGGCCGTTGCATGGGCGGCGTGGTTGCCGGTCGCAGCGAACAGATGAAA
GAAGTCGTCGGCTTCCTGCGTACCGCCGGGCCGACCCTCAGCCCGTTCAACGCCTGGATCTTCCTCAAGGGGCTGGAAAC
CCTGAACCTGCGGATGAAGGCGCACTGCGCCAATGCCCAACAACTGGCTGAGTGGCTGGAGCAGCAGGATGGCATCGAGA
AGGTGCATTACGCCGGTCTCAAGAGCCATCCGCAGCACGAACTGGCTCAGCGTCAGCAGAAGGGCTTCGGCGCGGTGGTG
AGCTTTGAGGTCAAGGGCGGCAAAGAGGGTGCCTGGCGTTTCATCGATGCGACCCGTTTGATCTCGATTACTGCCAACCT
CGGTGACAGCAAAACCACCATCACCCACCCGAGCACCACGTCCCATGGCCGTCTGGCGCCGCAGGAGCGTGAGGCAGCCG
GCATTCGTGACAGCCTGATCCGCATCGCGGTCGGTCTGGAAGACGTGGCTGACCTGCAAGCCGACCTGTCGCGCGGTCTG
GCGGCGTTGTGA

Upstream 100 bases:

>100_bases
TCGATCTGTACAACAACTGAGTTTCAACCGGCCCTTAGGGGCCGGTTTTGTATCTGGCAAAAGACTTTTATTTATCGAGA
ACAGGGCAAGGAGTGACAGC

Downstream 100 bases:

>100_bases
TCGAGTTGTCTACGCCAAAGATCGGCACCCATGGCCGCGTTGCATTGGTCACGGGTGCCGCGCGCGGGATCGGCTTGGGC
ATCGCGGCATGGCTGATCAG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSQEWDAGRLDSDLEGVAFDTLAVRAGQHRTPEGEHGDPMFFTSSYVFRTAADAAARFAGEVPGNVYSRYTNPTVRAFEE
RIAALESAEQAVATATGMAAIMAVVMSLCSAGDHVLVSRSVFGSTISLFEKYFKRFGVEVDYVPLADLSAWGAAIKSNTK
LLFVESPSNPLAELVDITALSEIAHAKGAMLVVDNCFCTPALQQPLKLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMKAHCANAQQLAEWLEQQDGIEKVHYAGLKSHPQHELAQRQQKGFGAVV
SFEVKGGKEGAWRFIDATRLISITANLGDSKTTITHPSTTSHGRLAPQEREAAGIRDSLIRIAVGLEDVADLQADLSRGL
AAL

Sequences:

>Translated_403_residues
MSQEWDAGRLDSDLEGVAFDTLAVRAGQHRTPEGEHGDPMFFTSSYVFRTAADAAARFAGEVPGNVYSRYTNPTVRAFEE
RIAALESAEQAVATATGMAAIMAVVMSLCSAGDHVLVSRSVFGSTISLFEKYFKRFGVEVDYVPLADLSAWGAAIKSNTK
LLFVESPSNPLAELVDITALSEIAHAKGAMLVVDNCFCTPALQQPLKLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMKAHCANAQQLAEWLEQQDGIEKVHYAGLKSHPQHELAQRQQKGFGAVV
SFEVKGGKEGAWRFIDATRLISITANLGDSKTTITHPSTTSHGRLAPQEREAAGIRDSLIRIAVGLEDVADLQADLSRGL
AAL
>Mature_402_residues
SQEWDAGRLDSDLEGVAFDTLAVRAGQHRTPEGEHGDPMFFTSSYVFRTAADAAARFAGEVPGNVYSRYTNPTVRAFEER
IAALESAEQAVATATGMAAIMAVVMSLCSAGDHVLVSRSVFGSTISLFEKYFKRFGVEVDYVPLADLSAWGAAIKSNTKL
LFVESPSNPLAELVDITALSEIAHAKGAMLVVDNCFCTPALQQPLKLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMKE
VVGFLRTAGPTLSPFNAWIFLKGLETLNLRMKAHCANAQQLAEWLEQQDGIEKVHYAGLKSHPQHELAQRQQKGFGAVVS
FEVKGGKEGAWRFIDATRLISITANLGDSKTTITHPSTTSHGRLAPQEREAAGIRDSLIRIAVGLEDVADLQADLSRGLA
AL

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=36.280487804878, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=32.9268292682927, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=30.8868501529052, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=37.5, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789383, Length=348, Percent_Identity=27.0114942528736, Blast_Score=124, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538055, Length=383, Percent_Identity=37.0757180156658, Blast_Score=245, Evalue=3e-65,
Organism=Caenorhabditis elegans, GI17560056, Length=325, Percent_Identity=40, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=34.5945945945946, Blast_Score=215, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=396, Percent_Identity=35.3535353535354, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=34.7126436781609, Blast_Score=225, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321254, Length=411, Percent_Identity=27.4939172749392, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=27.1341463414634, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=345, Percent_Identity=27.2463768115942, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922554, Length=344, Percent_Identity=36.3372093023256, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43366; Mature: 43234

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEWDAGRLDSDLEGVAFDTLAVRAGQHRTPEGEHGDPMFFTSSYVFRTAADAAARFAG
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCC
EVPGNVYSRYTNPTVRAFEERIAALESAEQAVATATGMAAIMAVVMSLCSAGDHVLVSRS
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
VFGSTISLFEKYFKRFGVEVDYVPLADLSAWGAAIKSNTKLLFVESPSNPLAELVDITAL
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
SEIAHAKGAMLVVDNCFCTPALQQPLKLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
HHHHHHCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHCCCCCCEEECEECCCHHHHH
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMKAHCANAQQLAEWLEQQDGIEKVHYAGL
HHHHHHHHCCCCCCCCCEEEEEECCHHHCEEHHHHHCCHHHHHHHHHHCCCHHHHHHCCC
KSHPQHELAQRQQKGFGAVVSFEVKGGKEGAWRFIDATRLISITANLGDSKTTITHPSTT
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEHEEEEEEEECCCCCCCEEECCCCC
SHGRLAPQEREAAGIRDSLIRIAVGLEDVADLQADLSRGLAAL
CCCCCCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SQEWDAGRLDSDLEGVAFDTLAVRAGQHRTPEGEHGDPMFFTSSYVFRTAADAAARFAG
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCC
EVPGNVYSRYTNPTVRAFEERIAALESAEQAVATATGMAAIMAVVMSLCSAGDHVLVSRS
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHH
VFGSTISLFEKYFKRFGVEVDYVPLADLSAWGAAIKSNTKLLFVESPSNPLAELVDITAL
HHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
SEIAHAKGAMLVVDNCFCTPALQQPLKLGADIVVHSATKFIDGQGRCMGGVVAGRSEQMK
HHHHHHCCCEEEEECCCCCHHHHHHHHCCCCEEEEHHHHHCCCCCCEEECEECCCHHHHH
EVVGFLRTAGPTLSPFNAWIFLKGLETLNLRMKAHCANAQQLAEWLEQQDGIEKVHYAGL
HHHHHHHHCCCCCCCCCEEEEEECCHHHCEEHHHHHCCHHHHHHHHHHCCCHHHHHHCCC
KSHPQHELAQRQQKGFGAVVSFEVKGGKEGAWRFIDATRLISITANLGDSKTTITHPSTT
CCCCHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEHEEEEEEEECCCCCCCEEECCCCC
SHGRLAPQEREAAGIRDSLIRIAVGLEDVADLQADLSRGLAAL
CCCCCCCCHHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]