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The map label for this gene is purF [H]

Identifier: 77458129

GI number: 77458129

Start: 2172745

End: 2174250

Strand: Direct

Name: purF [H]

Synonym: Pfl01_1902

Alternate gene names: 77458129

Gene position: 2172745-2174250 (Clockwise)

Preceding gene: 77458128

Following gene: 77458130

Centisome position: 33.75

GC content: 60.16

Gene sequence:

>1506_bases
ATGTGTGGCATCGTCGGTATCGTCGGTAAGTCGAACGTCAATCAGGCGCTGTATGACGCGCTAACCGTGCTCCAGCACCG
CGGCCAGGACGCTGCCGGTATCGTGACCAGCCATGATGGCCGGTTGTTCTTGCGCAAGGACAATGGCCTGGTGCGTGACG
TGTTTCAACAGCGTCACATGCAGCGCCTGGTCGGCCACATGGGTATCGGCCACGTCCGTTACCCGACTGCCGGCAGCTCG
ACGTCGGCCGAGGCTCAGCCGTTCTACGTCAACTCGCCTTACGGCATCACCCTGGCGCACAACGGTAACCTGACCAACGT
TGAACAGTTGGCCAAAGAGATCTACGAATCCGATCTGCGTCACGTCAACACCAGCTCCGACTCGGAAGTGTTGCTGAACG
TGTTCGCCCACGAGCTGGCCCAGCGCGGCAAGCTGCAACCGACCGAAGAAGACGTGTTTGCCGCCGTGACCGACGTGCAC
AACCGTTGCGTCGGTGGTTACGCCGTTGTGGCGATGGTGACCGGTTACGGCATCGTCGGCTTCCGTGACCCGCACGGCAT
CCGCCCGATCGTGTTCGGTCAGCGTCACACCGACGAAGGCGTCGAGTACATGATCGCCTCCGAAAGCGTTTCGCTGGACG
TGCTTGGCTTCACTCTGATCCGCGACCTGGCACCGGGCGAAGCGGTCTACATCACTGAAGACGGCAAGCTGCACACTCGT
CAGTGCGCGACCAACCCGTCCCTGACTCCGTGCATCTTCGAACACGTCTACCTGGCGCGTCCGGATTCGATCATCGACGG
CGTGTCGGTCTACAAAGCCCGTCTGCGCATGGGTGAGAAGCTGGCCGAGAAGATCCTGCGCGAGCGTCCAGAACACGACA
TCGACGTGGTCATCCCGATTCCGGACACCAGTCGCACCGCAGCACTGGAACTGGCCAACCATCTGGGCGTGAAATTCCGC
GAAGGCTTCGTCAAGAACCGTTACATCGGCCGTACCTTCATCATGCCCGGCCAGGCTGCGCGCAAGAAATCGGTACGCCA
GAAGCTCAACGCCATCGAACTGGAATTCCGCGGCAAGAACGTGATGCTGGTGGACGACTCGATCGTTCGCGGCACCACCT
GCAAGCAGATCATCCAGATGGCCCGTGAAGCCGGCGCGAAAAACGTCTACTTCTGCTCGGCGGCTCCGGCTGTTCGCTTC
CCGAACGTGTACGGCATCGACATGCCGAGCGCTCACGAGCTGATCGCTCACAACCGTTCGACTCAGGATGTCGCCGACCT
GATCGGCGCCGACTGGCTGATCTATCAGGACCTGCCTGACTTGATCGAAGCGGTCGGTGGCGGCAAGATCAAGATCGAAA
ACTTCGATTGCGCAGTGTTTGACGGCAAGTACGTCACCGGCGATGTCGACGAGGCTTACCTGAACAAGATCGAACAGGCA
CGCAACGATGCCTCGAAGGTCAAGACCCAGGCGGTCAGTGCGATCATCGATCTGTACAACAACTGA

Upstream 100 bases:

>100_bases
TACCCGCTGATATTCCGTTCAAGGAGCAACTCTTGCCGTCGGCCAAAACGCCTCAGTGAGTGTTGTTCAGTTCAGATCCA
TTAAGTAGGGGTTGCGTCGC

Downstream 100 bases:

>100_bases
GTTTCAACCGGCCCTTAGGGGCCGGTTTTGTATCTGGCAAAAGACTTTTATTTATCGAGAACAGGGCAAGGAGTGACAGC
ATGAGTCAGGAATGGGATGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFQQRHMQRLVGHMGIGHVRYPTAGSS
TSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTSSDSEVLLNVFAHELAQRGKLQPTEEDVFAAVTDVH
NRCVGGYAVVAMVTGYGIVGFRDPHGIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
QCATNPSLTPCIFEHVYLARPDSIIDGVSVYKARLRMGEKLAEKILRERPEHDIDVVIPIPDTSRTAALELANHLGVKFR
EGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRF
PNVYGIDMPSAHELIAHNRSTQDVADLIGADWLIYQDLPDLIEAVGGGKIKIENFDCAVFDGKYVTGDVDEAYLNKIEQA
RNDASKVKTQAVSAIIDLYNN

Sequences:

>Translated_501_residues
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFQQRHMQRLVGHMGIGHVRYPTAGSS
TSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTSSDSEVLLNVFAHELAQRGKLQPTEEDVFAAVTDVH
NRCVGGYAVVAMVTGYGIVGFRDPHGIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
QCATNPSLTPCIFEHVYLARPDSIIDGVSVYKARLRMGEKLAEKILRERPEHDIDVVIPIPDTSRTAALELANHLGVKFR
EGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRF
PNVYGIDMPSAHELIAHNRSTQDVADLIGADWLIYQDLPDLIEAVGGGKIKIENFDCAVFDGKYVTGDVDEAYLNKIEQA
RNDASKVKTQAVSAIIDLYNN
>Mature_501_residues
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFQQRHMQRLVGHMGIGHVRYPTAGSS
TSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLRHVNTSSDSEVLLNVFAHELAQRGKLQPTEEDVFAAVTDVH
NRCVGGYAVVAMVTGYGIVGFRDPHGIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
QCATNPSLTPCIFEHVYLARPDSIIDGVSVYKARLRMGEKLAEKILRERPEHDIDVVIPIPDTSRTAALELANHLGVKFR
EGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKNVMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRF
PNVYGIDMPSAHELIAHNRSTQDVADLIGADWLIYQDLPDLIEAVGGGKIKIENFDCAVFDGKYVTGDVDEAYLNKIEQA
RNDASKVKTQAVSAIIDLYNN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=475, Percent_Identity=34.9473684210526, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=63.220675944334, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=31.2138728323699, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=34.0248962655602, Blast_Score=238, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6323958, Length=512, Percent_Identity=53.3203125, Blast_Score=525, Evalue=1e-150,
Organism=Drosophila melanogaster, GI24659598, Length=485, Percent_Identity=34.2268041237113, Blast_Score=249, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24659604, Length=476, Percent_Identity=34.6638655462185, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI28573187, Length=504, Percent_Identity=33.3333333333333, Blast_Score=241, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55249; Mature: 55249

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFQQRHM
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHH
QRLVGHMGIGHVRYPTAGSSTSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLR
HHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTSSDSEVLLNVFAHELAQRGKLQPTEEDVFAAVTDVHNRCVGGYAVVAMVTGYGIVG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEE
FRDPHGIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
ECCCCCCCCEEECCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCEEEH
QCATNPSLTPCIFEHVYLARPDSIIDGVSVYKARLRMGEKLAEKILRERPEHDIDVVIPI
HCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTAALELANHLGVKFREGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKN
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCEEEEEECCCE
VMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRFPNVYGIDMPSAHELIAHNRS
EEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHCCCC
TQDVADLIGADWLIYQDLPDLIEAVGGGKIKIENFDCAVFDGKYVTGDVDEAYLNKIEQA
HHHHHHHHCCCEEEEHHHHHHHHHCCCCEEEEECCEEEEECCEEEECCCCHHHHHHHHHH
RNDASKVKTQAVSAIIDLYNN
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIVGIVGKSNVNQALYDALTVLQHRGQDAAGIVTSHDGRLFLRKDNGLVRDVFQQRHM
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHH
QRLVGHMGIGHVRYPTAGSSTSAEAQPFYVNSPYGITLAHNGNLTNVEQLAKEIYESDLR
HHHHHHCCCCCEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHH
HVNTSSDSEVLLNVFAHELAQRGKLQPTEEDVFAAVTDVHNRCVGGYAVVAMVTGYGIVG
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEE
FRDPHGIRPIVFGQRHTDEGVEYMIASESVSLDVLGFTLIRDLAPGEAVYITEDGKLHTR
ECCCCCCCCEEECCCCCCCCCEEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCEEEH
QCATNPSLTPCIFEHVYLARPDSIIDGVSVYKARLRMGEKLAEKILRERPEHDIDVVIPI
HCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PDTSRTAALELANHLGVKFREGFVKNRYIGRTFIMPGQAARKKSVRQKLNAIELEFRGKN
CCCCHHHHHHHHHHCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCEEEEEECCCE
VMLVDDSIVRGTTCKQIIQMAREAGAKNVYFCSAAPAVRFPNVYGIDMPSAHELIAHNRS
EEEECCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHCCCC
TQDVADLIGADWLIYQDLPDLIEAVGGGKIKIENFDCAVFDGKYVTGDVDEAYLNKIEQA
HHHHHHHHCCCEEEEHHHHHHHHHCCCCEEEEECCEEEEECCEEEECCCCHHHHHHHHHH
RNDASKVKTQAVSAIIDLYNN
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]