Click here to switch to the map view.

The map label for this gene is dac [H]

Identifier: 77458017

GI number: 77458017

Start: 1994466

End: 1995926

Strand: Reverse

Name: dac [H]

Synonym: Pfl01_1790

Alternate gene names: 77458017

Gene position: 1995926-1994466 (Counterclockwise)

Preceding gene: 77458019

Following gene: 77458014

Centisome position: 31.0

GC content: 62.97

Gene sequence:

>1461_bases
ATGATCAAATCGTTGCGTCCTCTGCTCCTGGCCGGTCTTCTTCTGCCGCTGGCCCTGCCCGTCTCCGCTGCCACCATCAA
CACCGCCCTGACCCCCAACGTCGAAAAAGCCCTCAAGGCCAGCAAGCTGCAGCCCTCTGCCCTGTCGCTGGTGATGGTGC
CGCTGGACGGCCCGGGCACGCCGACCGTGTACAACGCCGATGTGTCGGTCAACCCGGCCTCGACCATGAAGCTGGTCACC
ACTTACGCGGCGCTGGAAATGCTCGGCCCCAACCATCAGTGGAAAACCGAGTTCTATACCGACGGCGACCTGAACGGCGG
GATCCTCAACGGCAACCTCTACCTCAAGGGTGGCGGCGATCCGAAGCTGAACATGGAAAAACTCTGGCTGCTGATGCGCG
ACCTGCGCGCCAACGGCGTGACCCAGGTCACCGGTGATCTGGTACTCGATCGCAGCTTCTTCGTGCAGCCGCAACTGCCG
GAATTCAACGATGACGGCAACGACGAGAACAAGCCGTTCCTGGTCAAGCCGGATTCGCTGCTGGTCAACCTCAAGGCCTT
GCGTTTCGTGGCCCGCAACGACAACGGCCGGGTGCTGATCTCGGTCGAACCGCCGATTGCCAGCATCAACATCGAAAACA
CTGTCAAAGCGCTCAACTCCAAACAATGCACCGGCGGCGTGCGCTACAACCCGGTGCCGCAGGCCGATGGCAGCGTGACC
GTGACCGTGGCCGGCCAGTTGGGCGAAGGCTGCAGCTCGCAGACTTACCTGTCGCTGCTTGACCACGCGACCTACACCGC
CGGCGCCGTGCGGGCGATCTGGAAAGAATTGGGCGGCAGCATCCAGGGCAAGGATCGTCTGGCCCCGACCCCGAGCAGCG
CGAAAATGCTGGCCCGTGCGTTCTCGCCGGATCTGGCGGAAATCATCCGCGACATCAACAAATACAGTAACAACACCATG
GCTCAGCAGCTGTTCCTGAGCCTGGGCCAGAAATTCCGCAACGACGCCGACGGCGATGACGCCAAAGCCGCGCAACGGGT
GGTGCGTCAGTGGCTGGCGAAGAAAGGCATCACCGCGCCGCATCTGGTGATGGAGAACGGTTCCGGTCTGTCGCGTTCCG
AGCGGGTCAGCGCCCGGGAAATGGCCAACATGCTGCAAGCGGCCTGGCACAGCCCTTACGCCGCCGAGTACATCAGCTCG
CTGCCGATTGCCGGCACCGACGGCACCATGCGCAAACGCCTGAAGACCACCGCGATGCGCGGAGAGGCCCACGTCAAGAC
CGGCACTCTGAACACGGTACGGGCAATCGCCGGCTTCAGCCGCGACGTCAACGGCAACACTTGGGCGGTGGTGGCGATCC
TCAACGACAAGGCCCCGTTCGGCGCGTCGTCGGTGCTGGATCAGGTACTGCTGGATCTCTATAAACAGCCGCGGACGGCG
CAGACTGCTTCGGTTCTGTAA

Upstream 100 bases:

>100_bases
TTGCACCTTGGGGCATAATGCCGACACTTTCATGACCACTGAGGGCCTTTTCATCCATGTCCTCGTCTTTTTTCCCCTCG
GCTGTAGGGTTTTATTTCAT

Downstream 100 bases:

>100_bases
GCACACCACAAAACACTGTGGGAGCGACGGTGCGACGACTCGACTTGCTCGCGAAGACGTCCATTCAGTCGATGAAGATG
TCGACTTTGATGGCCCCTTC

Product: D-alanyl-D-alanine carboxypeptidase PBP3

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein; PBP [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MIKSLRPLLLAGLLLPLALPVSAATINTALTPNVEKALKASKLQPSALSLVMVPLDGPGTPTVYNADVSVNPASTMKLVT
TYAALEMLGPNHQWKTEFYTDGDLNGGILNGNLYLKGGGDPKLNMEKLWLLMRDLRANGVTQVTGDLVLDRSFFVQPQLP
EFNDDGNDENKPFLVKPDSLLVNLKALRFVARNDNGRVLISVEPPIASINIENTVKALNSKQCTGGVRYNPVPQADGSVT
VTVAGQLGEGCSSQTYLSLLDHATYTAGAVRAIWKELGGSIQGKDRLAPTPSSAKMLARAFSPDLAEIIRDINKYSNNTM
AQQLFLSLGQKFRNDADGDDAKAAQRVVRQWLAKKGITAPHLVMENGSGLSRSERVSAREMANMLQAAWHSPYAAEYISS
LPIAGTDGTMRKRLKTTAMRGEAHVKTGTLNTVRAIAGFSRDVNGNTWAVVAILNDKAPFGASSVLDQVLLDLYKQPRTA
QTASVL

Sequences:

>Translated_486_residues
MIKSLRPLLLAGLLLPLALPVSAATINTALTPNVEKALKASKLQPSALSLVMVPLDGPGTPTVYNADVSVNPASTMKLVT
TYAALEMLGPNHQWKTEFYTDGDLNGGILNGNLYLKGGGDPKLNMEKLWLLMRDLRANGVTQVTGDLVLDRSFFVQPQLP
EFNDDGNDENKPFLVKPDSLLVNLKALRFVARNDNGRVLISVEPPIASINIENTVKALNSKQCTGGVRYNPVPQADGSVT
VTVAGQLGEGCSSQTYLSLLDHATYTAGAVRAIWKELGGSIQGKDRLAPTPSSAKMLARAFSPDLAEIIRDINKYSNNTM
AQQLFLSLGQKFRNDADGDDAKAAQRVVRQWLAKKGITAPHLVMENGSGLSRSERVSAREMANMLQAAWHSPYAAEYISS
LPIAGTDGTMRKRLKTTAMRGEAHVKTGTLNTVRAIAGFSRDVNGNTWAVVAILNDKAPFGASSVLDQVLLDLYKQPRTA
QTASVL
>Mature_486_residues
MIKSLRPLLLAGLLLPLALPVSAATINTALTPNVEKALKASKLQPSALSLVMVPLDGPGTPTVYNADVSVNPASTMKLVT
TYAALEMLGPNHQWKTEFYTDGDLNGGILNGNLYLKGGGDPKLNMEKLWLLMRDLRANGVTQVTGDLVLDRSFFVQPQLP
EFNDDGNDENKPFLVKPDSLLVNLKALRFVARNDNGRVLISVEPPIASINIENTVKALNSKQCTGGVRYNPVPQADGSVT
VTVAGQLGEGCSSQTYLSLLDHATYTAGAVRAIWKELGGSIQGKDRLAPTPSSAKMLARAFSPDLAEIIRDINKYSNNTM
AQQLFLSLGQKFRNDADGDDAKAAQRVVRQWLAKKGITAPHLVMENGSGLSRSERVSAREMANMLQAAWHSPYAAEYISS
LPIAGTDGTMRKRLKTTAMRGEAHVKTGTLNTVRAIAGFSRDVNGNTWAVVAILNDKAPFGASSVLDQVLLDLYKQPRTA
QTASVL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=473, Percent_Identity=26.8498942917548, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52321; Mature: 52321

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSLRPLLLAGLLLPLALPVSAATINTALTPNVEKALKASKLQPSALSLVMVPLDGPGT
CCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCC
PTVYNADVSVNPASTMKLVTTYAALEMLGPNHQWKTEFYTDGDLNGGILNGNLYLKGGGD
CEEEECCEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEECEEEEECCCC
PKLNMEKLWLLMRDLRANGVTQVTGDLVLDRSFFVQPQLPEFNDDGNDENKPFLVKPDSL
CCCCHHHHHHHHHHHHCCCCEEECCCEEECCCEEECCCCCCCCCCCCCCCCCEEECCHHH
LVNLKALRFVARNDNGRVLISVEPPIASINIENTVKALNSKQCTGGVRYNPVPQADGSVT
EEEHHHHEEEEECCCCEEEEEECCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEE
VTVAGQLGEGCSSQTYLSLLDHATYTAGAVRAIWKELGGSIQGKDRLAPTPSSAKMLARA
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
FSPDLAEIIRDINKYSNNTMAQQLFLSLGQKFRNDADGDDAKAAQRVVRQWLAKKGITAP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
HLVMENGSGLSRSERVSAREMANMLQAAWHSPYAAEYISSLPIAGTDGTMRKRLKTTAMR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GEAHVKTGTLNTVRAIAGFSRDVNGNTWAVVAILNDKAPFGASSVLDQVLLDLYKQPRTA
CCCEEECCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
QTASVL
HHHCCC
>Mature Secondary Structure
MIKSLRPLLLAGLLLPLALPVSAATINTALTPNVEKALKASKLQPSALSLVMVPLDGPGT
CCCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCC
PTVYNADVSVNPASTMKLVTTYAALEMLGPNHQWKTEFYTDGDLNGGILNGNLYLKGGGD
CEEEECCEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEECEEEEECCCC
PKLNMEKLWLLMRDLRANGVTQVTGDLVLDRSFFVQPQLPEFNDDGNDENKPFLVKPDSL
CCCCHHHHHHHHHHHHCCCCEEECCCEEECCCEEECCCCCCCCCCCCCCCCCEEECCHHH
LVNLKALRFVARNDNGRVLISVEPPIASINIENTVKALNSKQCTGGVRYNPVPQADGSVT
EEEHHHHEEEEECCCCEEEEEECCCEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCCEE
VTVAGQLGEGCSSQTYLSLLDHATYTAGAVRAIWKELGGSIQGKDRLAPTPSSAKMLARA
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
FSPDLAEIIRDINKYSNNTMAQQLFLSLGQKFRNDADGDDAKAAQRVVRQWLAKKGITAP
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCC
HLVMENGSGLSRSERVSAREMANMLQAAWHSPYAAEYISSLPIAGTDGTMRKRLKTTAMR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHC
GEAHVKTGTLNTVRAIAGFSRDVNGNTWAVVAILNDKAPFGASSVLDQVLLDLYKQPRTA
CCCEEECCCHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
QTASVL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1554361; 7305936 [H]