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The map label for this gene is rlmL

Identifier: 77458015

GI number: 77458015

Start: 1989522

End: 1991792

Strand: Direct

Name: rlmL

Synonym: Pfl01_1788

Alternate gene names: 77458015

Gene position: 1989522-1991792 (Clockwise)

Preceding gene: 77458013

Following gene: 77458016

Centisome position: 30.9

GC content: 63.06

Gene sequence:

>2271_bases
ATGTCCGACCGTTTCGAACTCTTCCTCACTTGCCCCAAAGGCCTTGAAGGCCTGCTCATCGAGGAAGCCGTCGGGCTTGG
CCTTGAAGAGGCCCGCGAACACACTTCCGCCGTGCGTGGCATGGCGACCATGGAAACCGCTTATCGCCTGTGCCTCTGGT
CGCGTCTGGCCAACCGGGTGCTGCTGGTGCTCAAGCGCTTCCCGATGAAAAACGCTGAAGACCTGTACCACGGCGTACTC
GACGTCGACTGGCAGGATCACATGCTGGCCGACGGCACCCTGGCGGTGGAATTCAGCGGCCACGGTTCGGGCATCGACAA
CACTCACTTCGGCGCCCTGAAGGTCAAGGACGCCATCGTCGACAAACTGCGCACACCGCAGGGCGACCGCCCGAGCATCG
ACAAACTCAACCCGGACCTGCGCATTCACCTGCGTCTGGATCGCGGCGAAGCGATTCTGTCCCTCGACCTGTCCGGCCAC
AGCCTGCACCAGCGCGGTTACCGCTTGCAGCAGGGCGCTGCGCCGCTGAAGGAGAACCTTGCAGCCGCGATCCTGATCCG
TTCCGGCTGGCCGCGCATTGCCGCCGAAGGTGGCGCGCTGGCTGACCCGATGTGCGGTGTCGGCACGTTCCTGGTCGAAG
CCGGTATGATCGCCGCCGACATGGCGCCGAACCTGCGTCGCGAACAGTGGGGCTTCACCGCCTGGCTCGGTCACGTGCCG
GCGTTGTGGAAAAAACTGCATGAAGAAGCCGTCGAGCGTGCTGCTGCCGGTCTGGCCAAGCCGCCGCTGTGGATTCGCGG
TTATGAAGCCGACCCACGGCTGATCCAACCGGGCCGCAACAACGTCGAACGCGCCGGCCTGAGCGAGTGGATCAAGATCT
ATCAGGGCGAAGTGGCGACGTTCGAACCGCGTCCGGATCAGAACCAGAAAGGTCTGGTCATCTGCAACCCGCCGTACGGC
GAGCGTCTGGGCGATGAAGCCAGCCTGTTGTACCTCTACCAGAACCTCGGCGAGCGTCTGCGTCAGGCCTGCCTGAACTG
GGAAGCGGCGGTGTTCACCGGCGCGCCGGACCTGGGCAAGCGCATGGGCATCCGCAGCCACAAGCAGTATTCGTTCTGGA
ACGGCGCGTTGCCGTGCAAGCTGCTGTTGATCAAAGTCCTGCCGGATCAGTTCGTCACCGGCGAGCGCCGCACCCCGGAA
CAACGTCAGGCCGAGCGCGAGCAAGCCGCTTACGACCAGACGCCGAACGAGCCGCAGGAGCGCAAGTTCAACAAGAACGG
CAACCCGATCAAACCGACGCCGGCCCCGGCGCCAGTGATCGAGCAACCGCGCCTGAGCGAAGGCGGGCAGATGTTTGCCA
ACCGCCTGCAAAAGAACCTCAAGGCCATGAGCAAGTGGGTCAAGCGTGAAGGCATCGATTGCTACCGCGTCTACGATGCG
GACATGCCGGAATACGCCATGGCCATCGACCTGTACCACGACTGGGTGCACGTTCAGGAATACGCCGCGCCGAAGTCGAT
CGACCCGGAAAAGGCCTCGATCCGCATGTTCGACGCCCTGGCGGCCATTCCGCAGGCGCTGAACATCGACAAGAGCCGCG
TGGTGGTCAAGCGCCGGGAGCGTCAGAGCGGCACCAAACAGTACGAGCGTCAGGCCGCCCAGGGCAAGTTCAATGAAGTG
ACCGAGGGCGGCGTGAAGTTGCTGGTCAACCTCACCGACTACCTCGACACCGGGCTGTTCCTCGATCACCGGCCGATGCG
TATGCGGATCCAGAAAGAGGCCGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCGACCGCTTCCGTCCACGCAG
CCAAGGGCGGCGCGCGCAGCACCACCAGTGTCGACCTGTCGAAAACCTACCTCGACTGGGCGCGCCGCAACCTGTCGCTC
AACGGTTTCTCCGACAAGAACCGTCTGGAGCAGGGCGATGTGATGGCGTGGCTGGAAAATTGCCGCGAAGAGTACGACCT
GATCTTCATCGATCCGCCGACCTTCTCCAACTCCAAGCGCATGGAAGGCATCTTCGACGTGCAGCGAGATCAGGTGCAGT
TGATCGACCTGGCCATGGCCCGACTGGCGAGCGGTGGGGTGTTGTATTTCTCCAACAACTTCCGCAAGTTCCAGCTTGAA
GACAACCTGGCCGAGCGCTACGCGGTCGAGGAAATCACCGCCAGCACCATCGACCCGGATTTCGCCCGTAACGGCAAGAT
CCACCGCGCCTGGAAAATCACGGCTCGTTGA

Upstream 100 bases:

>100_bases
CACCTTCGTGCCACGCGGGCGCTCTTGTAGAAAAGCGCCTGATTGAAAACCGGATCGGCAATGCGTTGCCGGTTCATCAG
CCAAAGGCTCTGGAAAAACC

Downstream 100 bases:

>100_bases
CACTTTGCAAAATTGGATCCACAGAGCCTTGATTTTTAAAGGCTCTTGGATCCGTTCAGAGCTAGCCAAATTAGTGGCTA
ATAGCTATAACTCACACACG

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 756; Mature: 755

Protein sequence:

>756_residues
MSDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMATMETAYRLCLWSRLANRVLLVLKRFPMKNAEDLYHGVL
DVDWQDHMLADGTLAVEFSGHGSGIDNTHFGALKVKDAIVDKLRTPQGDRPSIDKLNPDLRIHLRLDRGEAILSLDLSGH
SLHQRGYRLQQGAAPLKENLAAAILIRSGWPRIAAEGGALADPMCGVGTFLVEAGMIAADMAPNLRREQWGFTAWLGHVP
ALWKKLHEEAVERAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVATFEPRPDQNQKGLVICNPPYG
ERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGKRMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPE
QRQAEREQAAYDQTPNEPQERKFNKNGNPIKPTPAPAPVIEQPRLSEGGQMFANRLQKNLKAMSKWVKREGIDCYRVYDA
DMPEYAMAIDLYHDWVHVQEYAAPKSIDPEKASIRMFDALAAIPQALNIDKSRVVVKRRERQSGTKQYERQAAQGKFNEV
TEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQKEAAGKRFLNLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSL
NGFSDKNRLEQGDVMAWLENCREEYDLIFIDPPTFSNSKRMEGIFDVQRDQVQLIDLAMARLASGGVLYFSNNFRKFQLE
DNLAERYAVEEITASTIDPDFARNGKIHRAWKITAR

Sequences:

>Translated_756_residues
MSDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMATMETAYRLCLWSRLANRVLLVLKRFPMKNAEDLYHGVL
DVDWQDHMLADGTLAVEFSGHGSGIDNTHFGALKVKDAIVDKLRTPQGDRPSIDKLNPDLRIHLRLDRGEAILSLDLSGH
SLHQRGYRLQQGAAPLKENLAAAILIRSGWPRIAAEGGALADPMCGVGTFLVEAGMIAADMAPNLRREQWGFTAWLGHVP
ALWKKLHEEAVERAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVATFEPRPDQNQKGLVICNPPYG
ERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGKRMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPE
QRQAEREQAAYDQTPNEPQERKFNKNGNPIKPTPAPAPVIEQPRLSEGGQMFANRLQKNLKAMSKWVKREGIDCYRVYDA
DMPEYAMAIDLYHDWVHVQEYAAPKSIDPEKASIRMFDALAAIPQALNIDKSRVVVKRRERQSGTKQYERQAAQGKFNEV
TEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQKEAAGKRFLNLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSL
NGFSDKNRLEQGDVMAWLENCREEYDLIFIDPPTFSNSKRMEGIFDVQRDQVQLIDLAMARLASGGVLYFSNNFRKFQLE
DNLAERYAVEEITASTIDPDFARNGKIHRAWKITAR
>Mature_755_residues
SDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMATMETAYRLCLWSRLANRVLLVLKRFPMKNAEDLYHGVLD
VDWQDHMLADGTLAVEFSGHGSGIDNTHFGALKVKDAIVDKLRTPQGDRPSIDKLNPDLRIHLRLDRGEAILSLDLSGHS
LHQRGYRLQQGAAPLKENLAAAILIRSGWPRIAAEGGALADPMCGVGTFLVEAGMIAADMAPNLRREQWGFTAWLGHVPA
LWKKLHEEAVERAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVATFEPRPDQNQKGLVICNPPYGE
RLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGKRMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPEQ
RQAEREQAAYDQTPNEPQERKFNKNGNPIKPTPAPAPVIEQPRLSEGGQMFANRLQKNLKAMSKWVKREGIDCYRVYDAD
MPEYAMAIDLYHDWVHVQEYAAPKSIDPEKASIRMFDALAAIPQALNIDKSRVVVKRRERQSGTKQYERQAAQGKFNEVT
EGGVKLLVNLTDYLDTGLFLDHRPMRMRIQKEAAGKRFLNLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSLN
GFSDKNRLEQGDVMAWLENCREEYDLIFIDPPTFSNSKRMEGIFDVQRDQVQLIDLAMARLASGGVLYFSNNFRKFQLED
NLAERYAVEEITASTIDPDFARNGKIHRAWKITAR

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=753, Percent_Identity=43.6918990703851, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI145693111, Length=266, Percent_Identity=29.3233082706767, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_PSEPF (Q3KFC5)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347520.1
- ProteinModelPortal:   Q3KFC5
- SMR:   Q3KFC5
- STRING:   Q3KFC5
- GeneID:   3716586
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1788
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- ProtClustDB:   PRK11783
- BioCyc:   PFLU205922:PFL_1788-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 85282; Mature: 85151

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE ; PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMATMETAYRLCLWSRLANRV
CCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVLKRFPMKNAEDLYHGVLDVDWQDHMLADGTLAVEFSGHGSGIDNTHFGALKVKDAIV
HHHHHHCCCCCHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCEEEHHHHHH
DKLRTPQGDRPSIDKLNPDLRIHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
HHHCCCCCCCCCHHHCCCCEEEEEEECCCCEEEEEECCCCHHHHCCCHHHCCCCCHHHCC
AAAILIRSGWPRIAAEGGALADPMCGVGTFLVEAGMIAADMAPNLRREQWGFTAWLGHVP
EEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHCCHHHHHHHHH
ALWKKLHEEAVERAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVAT
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEE
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGK
ECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
RMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPEQRQAEREQAAYDQTPNEPQE
HCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
RKFNKNGNPIKPTPAPAPVIEQPRLSEGGQMFANRLQKNLKAMSKWVKREGIDCYRVYDA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DMPEYAMAIDLYHDWVHVQEYAAPKSIDPEKASIRMFDALAAIPQALNIDKSRVVVKRRE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RQSGTKQYERQAAQGKFNEVTEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQKEAAGKRFL
HHHHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHHHCCCCEECCCCHHHHHHHHHHHHHHH
NLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSLNGFSDKNRLEQGDVMAWLEN
HHEEEECCCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
CREEYDLIFIDPPTFSNSKRMEGIFDVQRDQVQLIDLAMARLASGGVLYFSNNFRKFQLE
HHHHCCEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEC
DNLAERYAVEEITASTIDPDFARNGKIHRAWKITAR
HHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCC
>Mature Secondary Structure 
SDRFELFLTCPKGLEGLLIEEAVGLGLEEAREHTSAVRGMATMETAYRLCLWSRLANRV
CCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLVLKRFPMKNAEDLYHGVLDVDWQDHMLADGTLAVEFSGHGSGIDNTHFGALKVKDAIV
HHHHHHCCCCCHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCCCCCCCCCEEEHHHHHH
DKLRTPQGDRPSIDKLNPDLRIHLRLDRGEAILSLDLSGHSLHQRGYRLQQGAAPLKENL
HHHCCCCCCCCCHHHCCCCEEEEEEECCCCEEEEEECCCCHHHHCCCHHHCCCCCHHHCC
AAAILIRSGWPRIAAEGGALADPMCGVGTFLVEAGMIAADMAPNLRREQWGFTAWLGHVP
EEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHCCHHHHHCCCCCHHHHCCHHHHHHHHH
ALWKKLHEEAVERAAAGLAKPPLWIRGYEADPRLIQPGRNNVERAGLSEWIKIYQGEVAT
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEE
FEPRPDQNQKGLVICNPPYGERLGDEASLLYLYQNLGERLRQACLNWEAAVFTGAPDLGK
ECCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
RMGIRSHKQYSFWNGALPCKLLLIKVLPDQFVTGERRTPEQRQAEREQAAYDQTPNEPQE
HCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHH
RKFNKNGNPIKPTPAPAPVIEQPRLSEGGQMFANRLQKNLKAMSKWVKREGIDCYRVYDA
HHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECC
DMPEYAMAIDLYHDWVHVQEYAAPKSIDPEKASIRMFDALAAIPQALNIDKSRVVVKRRE
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RQSGTKQYERQAAQGKFNEVTEGGVKLLVNLTDYLDTGLFLDHRPMRMRIQKEAAGKRFL
HHHHHHHHHHHHHCCCHHHHHCCCCEEEEEHHHHHCCCCEECCCCHHHHHHHHHHHHHHH
NLFCYTATASVHAAKGGARSTTSVDLSKTYLDWARRNLSLNGFSDKNRLEQGDVMAWLEN
HHEEEECCCEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
CREEYDLIFIDPPTFSNSKRMEGIFDVQRDQVQLIDLAMARLASGGVLYFSNNFRKFQLE
HHHHCCEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEC
DNLAERYAVEEITASTIDPDFARNGKIHRAWKITAR
HHHHHHHHHHHHHHHCCCCCHHCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA