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The map label for this gene is pleD [H]

Identifier: 77458016

GI number: 77458016

Start: 1991939

End: 1994323

Strand: Direct

Name: pleD [H]

Synonym: Pfl01_1789

Alternate gene names: 77458016

Gene position: 1991939-1994323 (Clockwise)

Preceding gene: 77458015

Following gene: 77458018

Centisome position: 30.94

GC content: 62.77

Gene sequence:

>2385_bases
ATGTCGTTGCACCCCGTGCGCCCGAAGATATTGGGTTTTATCAGCGAAGACGTCTCGGCCTGGCTGGTCGGGGTGCTGGT
ATTGCTCGCCGGCGGAATTCTTACGGGGCTGCTCGCCTGGGGCACCCTCAATCAGTTTCACAGCCAGTTGCGCCAACGCT
TTCAGCTGCTGGCCAACGAACGCTATAGCCGCATCGAAGAACGATTTCAGGATCAGGAGCAACGTCTCGATGGCCTGCGC
CGGTTCTTCGCCAACTCCGAATCGGTGTCCCGCGCCGAATTCGACGGTTACACCCATCCTCTATTGCTGCGCACCCAGGC
TTATTCGTTTGCCTTGCGGGTGAGCGGTGCCGAACGTGCCGCCTTCGAGCAGCGCGTGCGAGATGAGGGCCTGAGCACCT
TCAGCGTGCGCGAACTCAATGCGCGCGGCGAACTGCAACTGGCCTCGGCCCGCGATGAATACGTGGTGGTGTTGTACAGC
CAGACCCAGAGCCGGCTCGGTTCTCCGCTGGGTTACGACTTGCTGGCTCAGCCGTTGCGCCGCTCGACCCTGGAGCGCGC
CGACCAGCTTCGCAGTCTGGCAGTGTCGCAGCCGATGCATCTGGTCGGTATCGAGCCGGCATACGCACGCGGCGTATTGC
TGGTGGCACCTGTGTTACGCGAAGGCGAGCCCAGATCATACGGCTACGTGATGGCTGTCATCAGCATGCGTCAGCTGCTG
GCAGACGGCTTGCCGGATGCATTTCATGACTATCTGTCGGTGCGCATCCTCGATCTGTCCACCACTGATCAGCATGAAGT
GCTGTTCGAATCGACCAATGAACCGGCGCAGAGTGATCTGTCGGCGACCCGGCTGGTGCGCATGGCCGATCACGACTATC
AGGTAGATATCCTGCCCAGCGAAGCGTTCATGCAGGCCAACCATTCGTCGGTGGGCAGCGTAGTGGTGCTGGGCGGGTTG
CTCAGTCTGCTGCTGAGCGCGCTGCTTTATGTGCTGGTCAGCCAGCGCCAGCGTGCGTTGCGCATGGTCGAGTTGCGCAC
TCAGGAACTGCATGAGCGCGAGCAGGAACTGCGCGGCACCCACGGTCAGTTGCGCGGGGTGCTCAACGCGGCGACCCAGG
TTGCAATCATCGCCACCGACCTGCGCGGAGTGATCAACACTTTCAACCCCGGTGCCGAGCAGATGCTCGGTTATCACAGC
AACGAAGTGGTCGGCCACATGACCCTGGAAAACCTGCACTTTCCCCGGGAACTGGTGGCCCGCGCGGCGGAACTCAGCGC
CCGTTATGGCAAGGCCATTCCGACCTGTCAGGCGATGCTGGTGGAGGGCGGCGAAGTCGGCGGGCACGAAGCGCGGGAAT
GGACGCTGGTGCGCAAGGATGGCAGCCACCTGCCGGTGAACATGTTGGCCACCCCGGTGCTGGACGAACAAGGGCTGTGG
GTCGGCCATCTGGCGATCTGCATCGACATCACCGAGCGCAAGCGCGTGCATGAAGCCCTGGCCGCGCGGGACGTGCTGTT
GAAGAAGCTCAGTGCCCACGTTCCCGGCGGGATCTATCAATTCAAAATGGAGTTCGACGGACGCTTCAGCGTGATCTACG
CCAGCGACGGTATCCGCGAGATCTATGAGCTGGAGCCGGACGTGCTGTTGCTCAACGCCGAAGCGATCTTCACCCGCATC
CACCCGCAGGATGTCAGCCGCGTGCGCAAGTCGATCCGTGCTTCGGCGGACAATCTCAGCCCATGGCGTGAGGAGTATCG
TGTGCAATTGCCCGAGCGTGGCCTGCGCTGGGTTCGCGGCGAGGCGACGCCGGAGGAACTGCCGGGCGGCGGGGTGCTGT
GGCATGGCTATATTTCGGATATTTCCGACCTGAAACGGGTGGAAGAAGAGCTGCGCGCGCTGTCGGTGACCGATTCGCTG
ACCGGGATTCACAACCGGCGCTATTTCCAGGAGCGCCTGACCACCGAAATGGCCCGGGTCGAGCGCGGCGGAGGCGAGTT
GTCGGTGATCATGCTCGACATCGACCACTTCAAGCGGATCAACGATCAGTACGGTCACGCGGTAGGTGACCGCGTGTTGC
AGGCGGTCTGCGAGCGCATCGGCCATCGTCTGCGGCGTACCGACGTATTCTGCCGCTTGGGTGGCGAAGAGTTCATGGTG
CTGTGCCCGGACATCGACGGCGATCACGCCCACATGCTGGCCGAAGAGTTATGGCACAGCCTGCGCAGTGCACCGATCGA
CGTGGTGGGGGTGGTGACGGCCAGTTTCGGGATTGCCAGCTGGCGACCGGGAGAGGGCGCGGATGCGCTGTTGCTGCGGG
CGGATTCCGGGGTGTATGCGGCGAAGCAGGGCGGACGGGATCGGGTCGAGCGGCATATGAACTGA

Upstream 100 bases:

>100_bases
TGGATCCGTTCAGAGCTAGCCAAATTAGTGGCTAATAGCTATAACTCACACACGGCCGATGGGGTTTTTCCCGCAGATGC
TGACGTAGTGAGTGGCTTTT

Downstream 100 bases:

>100_bases
TCCGGTTTGCCGCTGAATCCCTGTGGGAGCGAGCTTGCTTGCGAAGGGGCCATCAAAGTCGACATCTTCATCGACTGAAT
GGACGTCTTCGCGAGCAAGT

Product: PAS/PAC sensor-containing diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 794; Mature: 793

Protein sequence:

>794_residues
MSLHPVRPKILGFISEDVSAWLVGVLVLLAGGILTGLLAWGTLNQFHSQLRQRFQLLANERYSRIEERFQDQEQRLDGLR
RFFANSESVSRAEFDGYTHPLLLRTQAYSFALRVSGAERAAFEQRVRDEGLSTFSVRELNARGELQLASARDEYVVVLYS
QTQSRLGSPLGYDLLAQPLRRSTLERADQLRSLAVSQPMHLVGIEPAYARGVLLVAPVLREGEPRSYGYVMAVISMRQLL
ADGLPDAFHDYLSVRILDLSTTDQHEVLFESTNEPAQSDLSATRLVRMADHDYQVDILPSEAFMQANHSSVGSVVVLGGL
LSLLLSALLYVLVSQRQRALRMVELRTQELHEREQELRGTHGQLRGVLNAATQVAIIATDLRGVINTFNPGAEQMLGYHS
NEVVGHMTLENLHFPRELVARAAELSARYGKAIPTCQAMLVEGGEVGGHEAREWTLVRKDGSHLPVNMLATPVLDEQGLW
VGHLAICIDITERKRVHEALAARDVLLKKLSAHVPGGIYQFKMEFDGRFSVIYASDGIREIYELEPDVLLLNAEAIFTRI
HPQDVSRVRKSIRASADNLSPWREEYRVQLPERGLRWVRGEATPEELPGGGVLWHGYISDISDLKRVEEELRALSVTDSL
TGIHNRRYFQERLTTEMARVERGGGELSVIMLDIDHFKRINDQYGHAVGDRVLQAVCERIGHRLRRTDVFCRLGGEEFMV
LCPDIDGDHAHMLAEELWHSLRSAPIDVVGVVTASFGIASWRPGEGADALLLRADSGVYAAKQGGRDRVERHMN

Sequences:

>Translated_794_residues
MSLHPVRPKILGFISEDVSAWLVGVLVLLAGGILTGLLAWGTLNQFHSQLRQRFQLLANERYSRIEERFQDQEQRLDGLR
RFFANSESVSRAEFDGYTHPLLLRTQAYSFALRVSGAERAAFEQRVRDEGLSTFSVRELNARGELQLASARDEYVVVLYS
QTQSRLGSPLGYDLLAQPLRRSTLERADQLRSLAVSQPMHLVGIEPAYARGVLLVAPVLREGEPRSYGYVMAVISMRQLL
ADGLPDAFHDYLSVRILDLSTTDQHEVLFESTNEPAQSDLSATRLVRMADHDYQVDILPSEAFMQANHSSVGSVVVLGGL
LSLLLSALLYVLVSQRQRALRMVELRTQELHEREQELRGTHGQLRGVLNAATQVAIIATDLRGVINTFNPGAEQMLGYHS
NEVVGHMTLENLHFPRELVARAAELSARYGKAIPTCQAMLVEGGEVGGHEAREWTLVRKDGSHLPVNMLATPVLDEQGLW
VGHLAICIDITERKRVHEALAARDVLLKKLSAHVPGGIYQFKMEFDGRFSVIYASDGIREIYELEPDVLLLNAEAIFTRI
HPQDVSRVRKSIRASADNLSPWREEYRVQLPERGLRWVRGEATPEELPGGGVLWHGYISDISDLKRVEEELRALSVTDSL
TGIHNRRYFQERLTTEMARVERGGGELSVIMLDIDHFKRINDQYGHAVGDRVLQAVCERIGHRLRRTDVFCRLGGEEFMV
LCPDIDGDHAHMLAEELWHSLRSAPIDVVGVVTASFGIASWRPGEGADALLLRADSGVYAAKQGGRDRVERHMN
>Mature_793_residues
SLHPVRPKILGFISEDVSAWLVGVLVLLAGGILTGLLAWGTLNQFHSQLRQRFQLLANERYSRIEERFQDQEQRLDGLRR
FFANSESVSRAEFDGYTHPLLLRTQAYSFALRVSGAERAAFEQRVRDEGLSTFSVRELNARGELQLASARDEYVVVLYSQ
TQSRLGSPLGYDLLAQPLRRSTLERADQLRSLAVSQPMHLVGIEPAYARGVLLVAPVLREGEPRSYGYVMAVISMRQLLA
DGLPDAFHDYLSVRILDLSTTDQHEVLFESTNEPAQSDLSATRLVRMADHDYQVDILPSEAFMQANHSSVGSVVVLGGLL
SLLLSALLYVLVSQRQRALRMVELRTQELHEREQELRGTHGQLRGVLNAATQVAIIATDLRGVINTFNPGAEQMLGYHSN
EVVGHMTLENLHFPRELVARAAELSARYGKAIPTCQAMLVEGGEVGGHEAREWTLVRKDGSHLPVNMLATPVLDEQGLWV
GHLAICIDITERKRVHEALAARDVLLKKLSAHVPGGIYQFKMEFDGRFSVIYASDGIREIYELEPDVLLLNAEAIFTRIH
PQDVSRVRKSIRASADNLSPWREEYRVQLPERGLRWVRGEATPEELPGGGVLWHGYISDISDLKRVEEELRALSVTDSLT
GIHNRRYFQERLTTEMARVERGGGELSVIMLDIDHFKRINDQYGHAVGDRVLQAVCERIGHRLRRTDVFCRLGGEEFMVL
CPDIDGDHAHMLAEELWHSLRSAPIDVVGVVTASFGIASWRPGEGADALLLRADSGVYAAKQGGRDRVERHMN

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=203, Percent_Identity=35.4679802955665, Blast_Score=110, Evalue=5e-25,
Organism=Escherichia coli, GI1787262, Length=158, Percent_Identity=37.3417721518987, Blast_Score=104, Evalue=3e-23,
Organism=Escherichia coli, GI1787541, Length=172, Percent_Identity=36.046511627907, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=33.1491712707182, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=31.2101910828025, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=31.2849162011173, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=33.1521739130435, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=32.7272727272727, Blast_Score=83, Evalue=6e-17,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=30.8176100628931, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787816, Length=160, Percent_Identity=31.25, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 88989; Mature: 88857

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50839 CHASE ; PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLHPVRPKILGFISEDVSAWLVGVLVLLAGGILTGLLAWGTLNQFHSQLRQRFQLLANE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYSRIEERFQDQEQRLDGLRRFFANSESVSRAEFDGYTHPLLLRTQAYSFALRVSGAERA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEHHEEEEEEECCCHHH
AFEQRVRDEGLSTFSVRELNARGELQLASARDEYVVVLYSQTQSRLGSPLGYDLLAQPLR
HHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCEEEEEEECHHHHCCCCCCHHHHHHHHH
RSTLERADQLRSLAVSQPMHLVGIEPAYARGVLLVAPVLREGEPRSYGYVMAVISMRQLL
HHHHHHHHHHHHHHHCCCCEEEECCHHHHCCHHEEEHHHHCCCCCCHHHHHHHHHHHHHH
ADGLPDAFHDYLSVRILDLSTTDQHEVLFESTNEPAQSDLSATRLVRMADHDYQVDILPS
HCCCCHHHHHHHEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECH
EAFMQANHSSVGSVVVLGGLLSLLLSALLYVLVSQRQRALRMVELRTQELHEREQELRGT
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HGQLRGVLNAATQVAIIATDLRGVINTFNPGAEQMLGYHSNEVVGHMTLENLHFPRELVA
HHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEHHHCCCCHHHHH
RAAELSARYGKAIPTCQAMLVEGGEVGGHEAREWTLVRKDGSHLPVNMLATPVLDEQGLW
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCE
VGHLAICIDITERKRVHEALAARDVLLKKLSAHVPGGIYQFKMEFDGRFSVIYASDGIRE
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCHHH
IYELEPDVLLLNAEAIFTRIHPQDVSRVRKSIRASADNLSPWREEYRVQLPERGLRWVRG
HHHCCCCEEEEEHHHHHEECCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHCC
EATPEELPGGGVLWHGYISDISDLKRVEEELRALSVTDSLTGIHNRRYFQERLTTEMARV
CCCHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGGGELSVIMLDIDHFKRINDQYGHAVGDRVLQAVCERIGHRLRRTDVFCRLGGEEFMV
HCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
LCPDIDGDHAHMLAEELWHSLRSAPIDVVGVVTASFGIASWRPGEGADALLLRADSGVYA
ECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
AKQGGRDRVERHMN
HHCCCHHHHHHCCC
>Mature Secondary Structure 
SLHPVRPKILGFISEDVSAWLVGVLVLLAGGILTGLLAWGTLNQFHSQLRQRFQLLANE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYSRIEERFQDQEQRLDGLRRFFANSESVSRAEFDGYTHPLLLRTQAYSFALRVSGAERA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEHHEEEEEEECCCHHH
AFEQRVRDEGLSTFSVRELNARGELQLASARDEYVVVLYSQTQSRLGSPLGYDLLAQPLR
HHHHHHHHCCCCEEEEEECCCCCCEEEECCCCCEEEEEEECHHHHCCCCCCHHHHHHHHH
RSTLERADQLRSLAVSQPMHLVGIEPAYARGVLLVAPVLREGEPRSYGYVMAVISMRQLL
HHHHHHHHHHHHHHHCCCCEEEECCHHHHCCHHEEEHHHHCCCCCCHHHHHHHHHHHHHH
ADGLPDAFHDYLSVRILDLSTTDQHEVLFESTNEPAQSDLSATRLVRMADHDYQVDILPS
HCCCCHHHHHHHEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECH
EAFMQANHSSVGSVVVLGGLLSLLLSALLYVLVSQRQRALRMVELRTQELHEREQELRGT
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HGQLRGVLNAATQVAIIATDLRGVINTFNPGAEQMLGYHSNEVVGHMTLENLHFPRELVA
HHHHHHHHHHHHHHEEEHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEEHHHCCCCHHHHH
RAAELSARYGKAIPTCQAMLVEGGEVGGHEAREWTLVRKDGSHLPVNMLATPVLDEQGLW
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCE
VGHLAICIDITERKRVHEALAARDVLLKKLSAHVPGGIYQFKMEFDGRFSVIYASDGIRE
EEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCEEEEEEECCCHHH
IYELEPDVLLLNAEAIFTRIHPQDVSRVRKSIRASADNLSPWREEYRVQLPERGLRWVRG
HHHCCCCEEEEEHHHHHEECCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHCC
EATPEELPGGGVLWHGYISDISDLKRVEEELRALSVTDSLTGIHNRRYFQERLTTEMARV
CCCHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERGGGELSVIMLDIDHFKRINDQYGHAVGDRVLQAVCERIGHRLRRTDVFCRLGGEEFMV
HCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
LCPDIDGDHAHMLAEELWHSLRSAPIDVVGVVTASFGIASWRPGEGADALLLRADSGVYA
ECCCCCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCEEEEEECCCEEE
AKQGGRDRVERHMN
HHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA