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The map label for this gene is prpB [H]

Identifier: 77457991

GI number: 77457991

Start: 1959137

End: 1960027

Strand: Direct

Name: prpB [H]

Synonym: Pfl01_1764

Alternate gene names: 77457991

Gene position: 1959137-1960027 (Clockwise)

Preceding gene: 77457990

Following gene: 77457992

Centisome position: 30.43

GC content: 63.64

Gene sequence:

>891_bases
ATGAGTTCCAACAAGAGCACTCCAGGCCAGCGTTTCCGCGATGCGGTCGCCAGCGAACATCCGCTGCAAGTGGTCGGCGC
GATCAACGCCAACCACGCGCTGCTGGCCAAGCGCGCCGGTTTCAAGGCGATCTACCTGTCGGGTGGCGGGGTGGCGGCCG
GCTCCCTCGGCGTGCCGGACCTGGGCATCACCGGCCTGGATGACGTGCTGACAGACGTGCGCCGCATCACCGACGTCTGC
GACCTGCCGCTGCTGGTGGACGTGGACACCGGTTTCGGAGCCTCGGCGTTCAACGTGGCCCGCACCGTCAAGTCGATGAT
CAAGTTCGGCGCGGCGGCGATTCACATCGAAGACCAGGTCGGCGCCAAGCGCTGCGGCCACCGTCCGAACAAAGAAATCG
TGACCCAGCAGGAAATGGTCGACCGCATCAAGGCCGCCGTCGATGCCCGCACCGACGACAGCTTCGTGATCATGGCCCGC
ACCGATGCGCTGGCCGTTGAGGGTCTGGAATCGGCACTGGATCGCGCCGCCGCGTGCATCGAGGCCGGCGCCGACATGAT
CTTCCCGGAAGCCATCACTGAGCTTGAGATGTACAAGCTGTTCGCCAGCCGCGTGAAAGCGCCGATTCTGGCCAACATCA
CCGAATTCGGCTCGACGCCGCTGTACACCACCGAGCAACTGGCGGGCGCCGACGTTTCGCTGGTGCTGTACCCGCTATCG
GCGTTCCGCGCGATGAACAAGGCTGCGGAAAACGTCTACACCGCGATCCGCCGCGACGGCACCCAACAGAATGTCATCGA
CACCATGCAGACTCGCATGGAGCTTTACGATCGCATCGATTACCACACCTTCGAGCAGAAGCTCGACGCGTTGTTTGCGG
CGAAGAAGTAA

Upstream 100 bases:

>100_bases
AATTGTTGATGCGCCGTCACATCGGCGCCTCGAAACGCAACATCGCCCGTCACTACCAGGACGGCGCCGACAACAAGACA
GCCACTGAACGAGGTGAGTC

Downstream 100 bases:

>100_bases
GCCGCAACCGTACTCCCTAATAAATTCAAGATTGGAGACAACAATGGCCGAAGCAAAAGTACTCAGTGGCGCCGGGCTCC
GGGGCCAGGTCGCCGGGCAA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MSSNKSTPGQRFRDAVASEHPLQVVGAINANHALLAKRAGFKAIYLSGGGVAAGSLGVPDLGITGLDDVLTDVRRITDVC
DLPLLVDVDTGFGASAFNVARTVKSMIKFGAAAIHIEDQVGAKRCGHRPNKEIVTQQEMVDRIKAAVDARTDDSFVIMAR
TDALAVEGLESALDRAAACIEAGADMIFPEAITELEMYKLFASRVKAPILANITEFGSTPLYTTEQLAGADVSLVLYPLS
AFRAMNKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK

Sequences:

>Translated_296_residues
MSSNKSTPGQRFRDAVASEHPLQVVGAINANHALLAKRAGFKAIYLSGGGVAAGSLGVPDLGITGLDDVLTDVRRITDVC
DLPLLVDVDTGFGASAFNVARTVKSMIKFGAAAIHIEDQVGAKRCGHRPNKEIVTQQEMVDRIKAAVDARTDDSFVIMAR
TDALAVEGLESALDRAAACIEAGADMIFPEAITELEMYKLFASRVKAPILANITEFGSTPLYTTEQLAGADVSLVLYPLS
AFRAMNKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK
>Mature_295_residues
SSNKSTPGQRFRDAVASEHPLQVVGAINANHALLAKRAGFKAIYLSGGGVAAGSLGVPDLGITGLDDVLTDVRRITDVCD
LPLLVDVDTGFGASAFNVARTVKSMIKFGAAAIHIEDQVGAKRCGHRPNKEIVTQQEMVDRIKAAVDARTDDSFVIMART
DALAVEGLESALDRAAACIEAGADMIFPEAITELEMYKLFASRVKAPILANITEFGSTPLYTTEQLAGADVSLVLYPLSA
FRAMNKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=287, Percent_Identity=73.5191637630662, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1790445, Length=260, Percent_Identity=27.3076923076923, Blast_Score=77, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71982926, Length=177, Percent_Identity=32.2033898305085, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17561814, Length=177, Percent_Identity=32.2033898305085, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 31958; Mature: 31827

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNKSTPGQRFRDAVASEHPLQVVGAINANHALLAKRAGFKAIYLSGGGVAAGSLGVPD
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEECCCCCCC
LGITGLDDVLTDVRRITDVCDLPLLVDVDTGFGASAFNVARTVKSMIKFGAAAIHIEDQV
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECHHH
GAKRCGHRPNKEIVTQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALDRAAACI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAITELEMYKLFASRVKAPILANITEFGSTPLYTTEQLAGADVSLVLYPLS
HHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHH
AFRAMNKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSNKSTPGQRFRDAVASEHPLQVVGAINANHALLAKRAGFKAIYLSGGGVAAGSLGVPD
CCCCCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCEEECCCCCCC
LGITGLDDVLTDVRRITDVCDLPLLVDVDTGFGASAFNVARTVKSMIKFGAAAIHIEDQV
CCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECHHH
GAKRCGHRPNKEIVTQQEMVDRIKAAVDARTDDSFVIMARTDALAVEGLESALDRAAACI
HHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
EAGADMIFPEAITELEMYKLFASRVKAPILANITEFGSTPLYTTEQLAGADVSLVLYPLS
HHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCHHHHHHHHH
AFRAMNKAAENVYTAIRRDGTQQNVIDTMQTRMELYDRIDYHTFEQKLDALFAAKK
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9006051; 11677609; 10482501; 14575713 [H]