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The map label for this gene is cobQ

Identifier: 77457873

GI number: 77457873

Start: 1836886

End: 1838352

Strand: Direct

Name: cobQ

Synonym: Pfl01_1646

Alternate gene names: 77457873

Gene position: 1836886-1838352 (Clockwise)

Preceding gene: 77457872

Following gene: 77457874

Centisome position: 28.53

GC content: 63.12

Gene sequence:

>1467_bases
ATGACCACATTAATGGTGCAGGGCACCACCTCCGACGCCGGCAAAAGCACGCTGGTGACGGCGCTGTGCCGCTGGGCCAC
CCGTCAGGGCGTTGCGGTGGTGCCATTCAAACCGCAGAACATGGCGCTCAACAGCGCGGTGACGGCCGACGGCGGCGAGA
TCGGTCGCGCGCAAGCGGTGCAGGCCCAGGCGGCGTTTCTCGAGCCGCACACCGACATGAATCCGGTGCTGCTCAAGCCC
AACAGTGACACCGGCGCCCAGGTCATCATCCACGGTCGTGCGGTCACCAGCATGAACGCCGTCGCCTATCACGACTACAA
AGCCATCGCCATGCAGGCCGTGCTCGCTTCCCACGAACGTTTGAGTGCCGCCTATCCGCTGGTGATGGTCGAAGGTGCGG
GGTCGCCGGCCGAGATCAATCTGCGCGCCGGTGACATCGCCAACATGGGCTTCGCCGAAGCGGTGGATTGCCCGGTGTTG
CTGATCGCCGACATCAATCGTGGCGGCGTGTTTGCCCATCTGGTGGGCACGCTGGAATTGCTGTCGCCGAGCGAACAGGC
ACGGATCAAAGGTTTCATCATCAACCGTTTTCGCGGCGACATCGCCTTGCTGCAACCGGGCCTCGACTGGCTGGAACAGC
GCACCGGCAAACCGGTGGTGGGCGTGCTGCCGTACGTGATGGACCTGCATCTGGAGGCCGAGGACGGCATTGACCAGCGC
CAGACCGACAAGGCCGAGCAAGTTTTGAAAGTGGTGGTGCCGGTGCTGCCGCGTATCAGCAACCACACCGATTTCGATCC
GTTGCGTCTGCATCCTCAGGTGGATCTGCAATTCATCGGCCCGGGGCAGGCGATTCCTCCGGCCGATCTGATCATTCTTC
CGGGCTCGAAAAGCGTGCGCAGTGATTTGGCGTATCTGCGGGCCAATGGCTGGGAATCGGCGATCCGCCGTCACCTGCGT
TACGGCGGCAAATTGCTGGGGATCTGCGGCGGCCTGCAAATGCTCGGCGAGCAGGTGCACGATCCGCTGGGCCTGGAAGG
CGCGCCGGGTTCCAGCGCCGGTCTGGGTTTGTTGGCGTTTGAAACGCAGCTCGAAGCCGAAAAGCAGTTGCGCAATGTGC
GTGGACGTCTGGCGCTGGAAGATGCAGAGGTCAGCGGTTATGAAATTCATGCCGGCGTGACCACGGGAGCGGCGCTGGAA
AAGGCTGCCGTGCATCTGGACGACGGCCGTTGCGACGGCGCGCAAAGTCTCGACGGGCAGATTTTCGGCACCTACCTGCA
TGGCCTGTTCGAATCGCCGGCGGCCAGTGCCGCGCTGTTGCGCTGGGCCGGCCTGTACGATGTACAGGAGGTGGATTACC
ACGGCCTGCGCGAACGCGATATCGAGCGGCTGGCGGATCTGGTGGAGAAGCACCTGGATACCGGGCTGTTACGTGAACTC
TGTGGAATCTCCCCATGCTCCAGTTGA

Upstream 100 bases:

>100_bases
GCAGCCTGCGTTTTGGGCTGCCCGCCGATGCCGCCGACGAGGCGCGTCTTGAGCAAGCCCTGCAAGCATTCGCCAAGCAT
TGCTCCATGGAAAAACCAGC

Downstream 100 bases:

>100_bases
TCCTCGGCGGCGCGCGCTCCGGCAAAAGTCGCCTGGCCGAAAAGCTCGCCACTGACAGCGCGCTGGCCGTGACGTACATC
GCCACCAGCCAGCCGCTGGA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 487

Protein sequence:

>488_residues
MTTLMVQGTTSDAGKSTLVTALCRWATRQGVAVVPFKPQNMALNSAVTADGGEIGRAQAVQAQAAFLEPHTDMNPVLLKP
NSDTGAQVIIHGRAVTSMNAVAYHDYKAIAMQAVLASHERLSAAYPLVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVL
LIADINRGGVFAHLVGTLELLSPSEQARIKGFIINRFRGDIALLQPGLDWLEQRTGKPVVGVLPYVMDLHLEAEDGIDQR
QTDKAEQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQAIPPADLIILPGSKSVRSDLAYLRANGWESAIRRHLR
YGGKLLGICGGLQMLGEQVHDPLGLEGAPGSSAGLGLLAFETQLEAEKQLRNVRGRLALEDAEVSGYEIHAGVTTGAALE
KAAVHLDDGRCDGAQSLDGQIFGTYLHGLFESPAASAALLRWAGLYDVQEVDYHGLRERDIERLADLVEKHLDTGLLREL
CGISPCSS

Sequences:

>Translated_488_residues
MTTLMVQGTTSDAGKSTLVTALCRWATRQGVAVVPFKPQNMALNSAVTADGGEIGRAQAVQAQAAFLEPHTDMNPVLLKP
NSDTGAQVIIHGRAVTSMNAVAYHDYKAIAMQAVLASHERLSAAYPLVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVL
LIADINRGGVFAHLVGTLELLSPSEQARIKGFIINRFRGDIALLQPGLDWLEQRTGKPVVGVLPYVMDLHLEAEDGIDQR
QTDKAEQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQAIPPADLIILPGSKSVRSDLAYLRANGWESAIRRHLR
YGGKLLGICGGLQMLGEQVHDPLGLEGAPGSSAGLGLLAFETQLEAEKQLRNVRGRLALEDAEVSGYEIHAGVTTGAALE
KAAVHLDDGRCDGAQSLDGQIFGTYLHGLFESPAASAALLRWAGLYDVQEVDYHGLRERDIERLADLVEKHLDTGLLREL
CGISPCSS
>Mature_487_residues
TTLMVQGTTSDAGKSTLVTALCRWATRQGVAVVPFKPQNMALNSAVTADGGEIGRAQAVQAQAAFLEPHTDMNPVLLKPN
SDTGAQVIIHGRAVTSMNAVAYHDYKAIAMQAVLASHERLSAAYPLVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVLL
IADINRGGVFAHLVGTLELLSPSEQARIKGFIINRFRGDIALLQPGLDWLEQRTGKPVVGVLPYVMDLHLEAEDGIDQRQ
TDKAEQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQAIPPADLIILPGSKSVRSDLAYLRANGWESAIRRHLRY
GGKLLGICGGLQMLGEQVHDPLGLEGAPGSSAGLGLLAFETQLEAEKQLRNVRGRLALEDAEVSGYEIHAGVTTGAALEK
AAVHLDDGRCDGAQSLDGQIFGTYLHGLFESPAASAALLRWAGLYDVQEVDYHGLRERDIERLADLVEKHLDTGLLRELC
GISPCSS

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_PSEPF (Q3KFR7)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_347378.1
- ProteinModelPortal:   Q3KFR7
- SMR:   Q3KFR7
- STRING:   Q3KFR7
- GeneID:   3716819
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1646
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   PFLU205922:PFL_1646-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 52243; Mature: 52112

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 329-329 ACT_SITE 427-427

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLMVQGTTSDAGKSTLVTALCRWATRQGVAVVPFKPQNMALNSAVTADGGEIGRAQAV
CCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEECCEEECCCCCCCHHHHH
QAQAAFLEPHTDMNPVLLKPNSDTGAQVIIHGRAVTSMNAVAYHDYKAIAMQAVLASHER
HHHHHHCCCCCCCCCEEECCCCCCCCEEEEECCEECCCCHHEECHHHHHHHHHHHHHHHH
LSAAYPLVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVLLIADINRGGVFAHLVGTLEL
HHCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHH
LSPSEQARIKGFIINRFRGDIALLQPGLDWLEQRTGKPVVGVLPYVMDLHLEAEDGIDQR
CCCCHHHHEEEEEEECCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCHH
QTDKAEQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQAIPPADLIILPGSKSVR
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECCCHHHH
SDLAYLRANGWESAIRRHLRYGGKLLGICGGLQMLGEQVHDPLGLEGAPGSSAGLGLLAF
HHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
ETQLEAEKQLRNVRGRLALEDAEVSGYEIHAGVTTGAALEKAAVHLDDGRCDGAQSLDGQ
HHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCCCHHHHHHEEEECCCCCCCCCCCCCH
IFGTYLHGLFESPAASAALLRWAGLYDVQEVDYHGLRERDIERLADLVEKHLDTGLLREL
HHHHHHHHHHCCCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHH
CGISPCSS
HCCCCCCC
>Mature Secondary Structure 
TTLMVQGTTSDAGKSTLVTALCRWATRQGVAVVPFKPQNMALNSAVTADGGEIGRAQAV
CEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEECCEEECCCCCCCHHHHH
QAQAAFLEPHTDMNPVLLKPNSDTGAQVIIHGRAVTSMNAVAYHDYKAIAMQAVLASHER
HHHHHHCCCCCCCCCEEECCCCCCCCEEEEECCEECCCCHHEECHHHHHHHHHHHHHHHH
LSAAYPLVMVEGAGSPAEINLRAGDIANMGFAEAVDCPVLLIADINRGGVFAHLVGTLEL
HHCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEEEECCCCCCHHHHHHHHHH
LSPSEQARIKGFIINRFRGDIALLQPGLDWLEQRTGKPVVGVLPYVMDLHLEAEDGIDQR
CCCCHHHHEEEEEEECCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCHH
QTDKAEQVLKVVVPVLPRISNHTDFDPLRLHPQVDLQFIGPGQAIPPADLIILPGSKSVR
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECCCHHHH
SDLAYLRANGWESAIRRHLRYGGKLLGICGGLQMLGEQVHDPLGLEGAPGSSAGLGLLAF
HHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
ETQLEAEKQLRNVRGRLALEDAEVSGYEIHAGVTTGAALEKAAVHLDDGRCDGAQSLDGQ
HHHHHHHHHHHHHHCCEEEECCCCCCEEEECCCCCCHHHHHHEEEECCCCCCCCCCCCCH
IFGTYLHGLFESPAASAALLRWAGLYDVQEVDYHGLRERDIERLADLVEKHLDTGLLREL
HHHHHHHHHHCCCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHH
CGISPCSS
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA