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The map label for this gene is epsM [H]

Identifier: 77457742

GI number: 77457742

Start: 1702381

End: 1703040

Strand: Direct

Name: epsM [H]

Synonym: Pfl01_1515

Alternate gene names: 77457742

Gene position: 1702381-1703040 (Clockwise)

Preceding gene: 77457741

Following gene: 77457743

Centisome position: 26.44

GC content: 62.73

Gene sequence:

>660_bases
ATGTATCTGCTGGGGGCCGCCAACCCGGAAGCGGTGCGCATGCTGCACGCGGTCAAGCGCAGTACGCCGAATGTCGAGTT
CGCCTTTCTGGACAACGATCCGCGCAAGCACGGCACGCTGTTTTACGGGGTGCCGGTGGTGGGTGGTTCGGAGCGGGTCG
CCGAGCTCAATGGCCCGGACGTGCGCTTCGTCAATCTGATCACCGGCAGTACCCGGTTACGCTACGAAACCACCTGTCAG
TTGGTGGAGGCAGGGGCGAAGCTCGGCCAGTTCGTTCATCCCGGTATCGACCTGAGCATGATCCGCATGGGGCAGGGCAG
TTACCTGCAGGAAGGCGTGTTGATGCAGGCCGAAGTCGAACTCGGCGACAACACCAGCATCAGCGCCGGTAGCGTCGTGG
GGCATGAAGGGCGGATCGGCCATTCAGTGTTCATGGCGCCGGGCGTGTGCATCGCCGGCTGTGTGGAAATCGGCGACGGT
ACGTTTATCGGTACCAACGCCACGATTCTGCCGCGCCTGCGCATCGGTCGCTGGGTGACCATCGGCGCCGGCGCTGTGGT
GACAAAGGACGTCCCGGATTTTTCGGTCGTGGTGGGCAATCCCGCCAGGATCATCAAGACCAACACCGTGCCATACCCTG
ACGCCAGGGTGTTCAGGTAA

Upstream 100 bases:

>100_bases
CCTTCAATCTGCCGAGCTATCACGACATGACCGACGCCGATCAGCAGCGCGTCGTGCAAGTGGTGCTGGACATGTTGGCA
GGCAGGCCCGCGCTGTGAAG

Downstream 100 bases:

>100_bases
GCCGTTTTCCCGAATTTTTGGAGAGTCGCAATGAATCCACATGAGCAGTTTCGCGAAGAAGTAAAAGCCAACATCGAAGG
CCTGCAACAGGACAAGGCGC

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MYLLGAANPEAVRMLHAVKRSTPNVEFAFLDNDPRKHGTLFYGVPVVGGSERVAELNGPDVRFVNLITGSTRLRYETTCQ
LVEAGAKLGQFVHPGIDLSMIRMGQGSYLQEGVLMQAEVELGDNTSISAGSVVGHEGRIGHSVFMAPGVCIAGCVEIGDG
TFIGTNATILPRLRIGRWVTIGAGAVVTKDVPDFSVVVGNPARIIKTNTVPYPDARVFR

Sequences:

>Translated_219_residues
MYLLGAANPEAVRMLHAVKRSTPNVEFAFLDNDPRKHGTLFYGVPVVGGSERVAELNGPDVRFVNLITGSTRLRYETTCQ
LVEAGAKLGQFVHPGIDLSMIRMGQGSYLQEGVLMQAEVELGDNTSISAGSVVGHEGRIGHSVFMAPGVCIAGCVEIGDG
TFIGTNATILPRLRIGRWVTIGAGAVVTKDVPDFSVVVGNPARIIKTNTVPYPDARVFR
>Mature_219_residues
MYLLGAANPEAVRMLHAVKRSTPNVEFAFLDNDPRKHGTLFYGVPVVGGSERVAELNGPDVRFVNLITGSTRLRYETTCQ
LVEAGAKLGQFVHPGIDLSMIRMGQGSYLQEGVLMQAEVELGDNTSISAGSVVGHEGRIGHSVFMAPGVCIAGCVEIGDG
TFIGTNATILPRLRIGRWVTIGAGAVVTKDVPDFSVVVGNPARIIKTNTVPYPDARVFR

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 23393; Mature: 23393

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLLGAANPEAVRMLHAVKRSTPNVEFAFLDNDPRKHGTLFYGVPVVGGSERVAELNGPD
CEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEECCCCEEEECCCCC
VRFVNLITGSTRLRYETTCQLVEAGAKLGQFVHPGIDLSMIRMGQGSYLQEGVLMQAEVE
EEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCEEEEEEEE
LGDNTSISAGSVVGHEGRIGHSVFMAPGVCIAGCVEIGDGTFIGTNATILPRLRIGRWVT
ECCCCCCCCCCEECCCCCCCCEEEECCCCEEEEEEECCCCEEECCCCEEEEEEECCCEEE
IGAGAVVTKDVPDFSVVVGNPARIIKTNTVPYPDARVFR
ECCCEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MYLLGAANPEAVRMLHAVKRSTPNVEFAFLDNDPRKHGTLFYGVPVVGGSERVAELNGPD
CEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEEEECCCCEEEECCCCC
VRFVNLITGSTRLRYETTCQLVEAGAKLGQFVHPGIDLSMIRMGQGSYLQEGVLMQAEVE
EEEEEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCEEEEEEEE
LGDNTSISAGSVVGHEGRIGHSVFMAPGVCIAGCVEIGDGTFIGTNATILPRLRIGRWVT
ECCCCCCCCCCEECCCCCCCCEEEECCCCEEEEEEECCCCEEECCCCEEEEEEECCCEEE
IGAGAVVTKDVPDFSVVVGNPARIIKTNTVPYPDARVFR
ECCCEEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 40 {triethanolamine}} 90 {maltose}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]