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The map label for this gene is epsN [H]

Identifier: 77457741

GI number: 77457741

Start: 1701257

End: 1702378

Strand: Direct

Name: epsN [H]

Synonym: Pfl01_1514

Alternate gene names: 77457741

Gene position: 1701257-1702378 (Clockwise)

Preceding gene: 77457740

Following gene: 77457742

Centisome position: 26.42

GC content: 63.28

Gene sequence:

>1122_bases
ATGAGCAGAATTCACTACACCAAACCCAGCGTCGGCGAACTCGAAGCGCAATATGCCCTGGATGCCGTGCAGAACGGCTG
GGGCGCGCGCTGCTACGAATACCTGACCCGCTTCGAGCACGGTTTTGCCGAGCACCTGGGCGCGACCTACGCCATCGCCA
CCTCCAGTTGCACCGGAGCCCTGCACATGGGCATGGCGGCGCTGGGCATCGGCGAAGGCGATGAAGTGATCCTGGGCAAC
ACCAACTGGATCGCCTCGGCGGCGCCAATCACTTATCTGGGCGCCACGCCGGTGTTCGTCGATGTGCTGGCCGACAGCTG
GTGCCTCGACCCGCAGCAGGTCAGAAACGCGATCAGCCCGCGCACCAAGGCCATTCTGGCGGTGCACCTGTACGGCAATC
TGTGTGACATGGATGCCTTGCTGGCCATCGGCAAGGAATTCGGGATTCCCGTGATCGAAGATGCGGCCGAAGCCATCGGT
TCGCAGTGGCGCGGTCAGGCGGCTGGTTCGATGGGAGCCTTCGGTGCTTTCTCGTTCCACGGCACCAAGACCATGACCAC
CGGCGAGGGCGGAATGTTCGTCACCTCGGACAAGGCCTTGTACGAGCGCGTGCTGACCCTGTCCAACCACGGTCGCGTGG
CGGGCTGCACCAAGCAGTTCTGGCCAGAGTTCGTCGGTTTCAAATACAAGATGAGCAACCTGCAGGCGGCCGTCGGTTGC
GCGCAGGTCGAGCGGATCGAAGAGTTGATCGCGCGCAAGCGGGCGATCTTTTCCAACTATGCGCAGGCGCTGGAAGGCGT
ACCGGGCGTGTCGCTCAATCCCGAGCCGGCTCACGGGCGCAACGGTTACTGGATGCCGACCGTGGTGTTCGATGCCGACA
CCGGTATCCATCGCGACCAGTTGATCGCGGCTTTCCAGGCGGCTGACATCGATGCCCGGGTGTTCTTCTGGCCGCTGTCG
ACGTTGCCGATGTTCAGCGGGCACAACGTTGACACCCCGGTGTCATTCGCCCTGCCGGAGCGCGCCTTCAATCTGCCGAG
CTATCACGACATGACCGACGCCGATCAGCAGCGCGTCGTGCAAGTGGTGCTGGACATGTTGGCAGGCAGGCCCGCGCTGT
GA

Upstream 100 bases:

>100_bases
TTCTGCCCTGGAACCTGTTGAGCGAAGTGACGCAACAACTGGCCGAAGCCAAAACATGGGGTGCACGCTTTGTCGTCGCC
GTTCCGGAGTTGAGCATTCA

Downstream 100 bases:

>100_bases
AGATGTATCTGCTGGGGGCCGCCAACCCGGAAGCGGTGCGCATGCTGCACGCGGTCAAGCGCAGTACGCCGAATGTCGAG
TTCGCCTTTCTGGACAACGA

Product: DegT/DnrJ/EryC1/StrS aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MSRIHYTKPSVGELEAQYALDAVQNGWGARCYEYLTRFEHGFAEHLGATYAIATSSCTGALHMGMAALGIGEGDEVILGN
TNWIASAAPITYLGATPVFVDVLADSWCLDPQQVRNAISPRTKAILAVHLYGNLCDMDALLAIGKEFGIPVIEDAAEAIG
SQWRGQAAGSMGAFGAFSFHGTKTMTTGEGGMFVTSDKALYERVLTLSNHGRVAGCTKQFWPEFVGFKYKMSNLQAAVGC
AQVERIEELIARKRAIFSNYAQALEGVPGVSLNPEPAHGRNGYWMPTVVFDADTGIHRDQLIAAFQAADIDARVFFWPLS
TLPMFSGHNVDTPVSFALPERAFNLPSYHDMTDADQQRVVQVVLDMLAGRPAL

Sequences:

>Translated_373_residues
MSRIHYTKPSVGELEAQYALDAVQNGWGARCYEYLTRFEHGFAEHLGATYAIATSSCTGALHMGMAALGIGEGDEVILGN
TNWIASAAPITYLGATPVFVDVLADSWCLDPQQVRNAISPRTKAILAVHLYGNLCDMDALLAIGKEFGIPVIEDAAEAIG
SQWRGQAAGSMGAFGAFSFHGTKTMTTGEGGMFVTSDKALYERVLTLSNHGRVAGCTKQFWPEFVGFKYKMSNLQAAVGC
AQVERIEELIARKRAIFSNYAQALEGVPGVSLNPEPAHGRNGYWMPTVVFDADTGIHRDQLIAAFQAADIDARVFFWPLS
TLPMFSGHNVDTPVSFALPERAFNLPSYHDMTDADQQRVVQVVLDMLAGRPAL
>Mature_372_residues
SRIHYTKPSVGELEAQYALDAVQNGWGARCYEYLTRFEHGFAEHLGATYAIATSSCTGALHMGMAALGIGEGDEVILGNT
NWIASAAPITYLGATPVFVDVLADSWCLDPQQVRNAISPRTKAILAVHLYGNLCDMDALLAIGKEFGIPVIEDAAEAIGS
QWRGQAAGSMGAFGAFSFHGTKTMTTGEGGMFVTSDKALYERVLTLSNHGRVAGCTKQFWPEFVGFKYKMSNLQAAVGCA
QVERIEELIARKRAIFSNYAQALEGVPGVSLNPEPAHGRNGYWMPTVVFDADTGIHRDQLIAAFQAADIDARVFFWPLST
LPMFSGHNVDTPVSFALPERAFNLPSYHDMTDADQQRVVQVVLDMLAGRPAL

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=376, Percent_Identity=34.5744680851064, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI2367285, Length=380, Percent_Identity=30.2631578947368, Blast_Score=159, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 40435; Mature: 40304

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIHYTKPSVGELEAQYALDAVQNGWGARCYEYLTRFEHGFAEHLGATYAIATSSCTGA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHH
LHMGMAALGIGEGDEVILGNTNWIASAAPITYLGATPVFVDVLADSWCLDPQQVRNAISP
HHHHHHHHCCCCCCEEEECCCCCEECCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHCC
RTKAILAVHLYGNLCDMDALLAIGKEFGIPVIEDAAEAIGSQWRGQAAGSMGAFGAFSFH
CHHEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
GTKTMTTGEGGMFVTSDKALYERVLTLSNHGRVAGCTKQFWPEFVGFKYKMSNLQAAVGC
CCEEEEECCCCEEEECCHHHHHHHHHHCCCCCEECCHHHHHHHHHCCEEEHHHHHHHHHH
AQVERIEELIARKRAIFSNYAQALEGVPGVSLNPEPAHGRNGYWMPTVVFDADTGIHRDQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECEEEEEECCCCCCHHH
LIAAFQAADIDARVFFWPLSTLPMFSGHNVDTPVSFALPERAFNLPSYHDMTDADQQRVV
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCHHHCCCCCCCCCCCHHHHHHH
QVVLDMLAGRPAL
HHHHHHHCCCCCC
>Mature Secondary Structure 
SRIHYTKPSVGELEAQYALDAVQNGWGARCYEYLTRFEHGFAEHLGATYAIATSSCTGA
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHH
LHMGMAALGIGEGDEVILGNTNWIASAAPITYLGATPVFVDVLADSWCLDPQQVRNAISP
HHHHHHHHCCCCCCEEEECCCCCEECCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHHCC
RTKAILAVHLYGNLCDMDALLAIGKEFGIPVIEDAAEAIGSQWRGQAAGSMGAFGAFSFH
CHHEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEC
GTKTMTTGEGGMFVTSDKALYERVLTLSNHGRVAGCTKQFWPEFVGFKYKMSNLQAAVGC
CCEEEEECCCCEEEECCHHHHHHHHHHCCCCCEECCHHHHHHHHHCCEEEHHHHHHHHHH
AQVERIEELIARKRAIFSNYAQALEGVPGVSLNPEPAHGRNGYWMPTVVFDADTGIHRDQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECEEEEEECCCCCCHHH
LIAAFQAADIDARVFFWPLSTLPMFSGHNVDTPVSFALPERAFNLPSYHDMTDADQQRVV
HHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCHHHCCCCCCCCCCCHHHHHHH
QVVLDMLAGRPAL
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]