Click here to switch to the map view.

The map label for this gene is 77457743

Identifier: 77457743

GI number: 77457743

Start: 1703071

End: 1703799

Strand: Direct

Name: 77457743

Synonym: Pfl01_1516

Alternate gene names: NA

Gene position: 1703071-1703799 (Clockwise)

Preceding gene: 77457742

Following gene: 77457744

Centisome position: 26.45

GC content: 57.61

Gene sequence:

>729_bases
ATGAATCCACATGAGCAGTTTCGCGAAGAAGTAAAAGCCAACATCGAAGGCCTGCAACAGGACAAGGCGCTGCAAACCGA
GTCTCTGGATTGGGTCGGCACTACGGCAAAACACAAGTACACCTACAACTTCAGCTGGATGGGCCGGCCGATCATTCAGT
TCCCGCAGGATATGGTCGCCATGCAGGAAATCATCTGGAACCTGCGTCCGGACATCATCGTGGAAACCGGTATCGCCCAC
GGCGGTTCGCTGGTGTTCTACGCCTCGATCCTCGAGCTGATCGGTCACGGCGAAGTCCTGGGCATCGATATCGACATCCG
CCAGCACAATCGCGAAGCCATCGAAGCGCACCCGATGTTCAAGCGGATCAGCATGATCCAGGGTTCGAGCATCGACACCG
CGATCGTCGACCAGGTGCGCGAACGCGTGCAGGGCAAGAAAGTGCTGGTGGTGCTGGATTCCAATCACACCCACGAGCAC
GTCCTCGAAGAGCTGCGTCTGTACGCACCGCTGGTGTCGGTGGGCAGCTACTGCGTGGTCATGGACACCGTGGTCGAAGA
CATGCCGCAAGACGCCTTCCCGGATCGTCCGTGGGGCAAGGGTGACAACCCGAAAACCGCTGTGTGGGCTTACCTGAAAG
AGAGCAGCGATTTCGAAATCGATCAGGCCATTCACAGCAAGTTGCTGATCACTGTCGCGCCGGATGGCTATCTGCGTCGC
GTTCGTTAA

Upstream 100 bases:

>100_bases
GGTGGGCAATCCCGCCAGGATCATCAAGACCAACACCGTGCCATACCCTGACGCCAGGGTGTTCAGGTAAGCCGTTTTCC
CGAATTTTTGGAGAGTCGCA

Downstream 100 bases:

>100_bases
TCGACAAAAACAACGTTGAGTTTTCGGCGACTCGTCGCTTGTGGGGAATTTATGCAAGGCAAGAACAGTTCTGGACACGG
TTTGGCACTCAACGAACAGT

Product: cephalosporin hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNPHEQFREEVKANIEGLQQDKALQTESLDWVGTTAKHKYTYNFSWMGRPIIQFPQDMVAMQEIIWNLRPDIIVETGIAH
GGSLVFYASILELIGHGEVLGIDIDIRQHNREAIEAHPMFKRISMIQGSSIDTAIVDQVRERVQGKKVLVVLDSNHTHEH
VLEELRLYAPLVSVGSYCVVMDTVVEDMPQDAFPDRPWGKGDNPKTAVWAYLKESSDFEIDQAIHSKLLITVAPDGYLRR
VR

Sequences:

>Translated_242_residues
MNPHEQFREEVKANIEGLQQDKALQTESLDWVGTTAKHKYTYNFSWMGRPIIQFPQDMVAMQEIIWNLRPDIIVETGIAH
GGSLVFYASILELIGHGEVLGIDIDIRQHNREAIEAHPMFKRISMIQGSSIDTAIVDQVRERVQGKKVLVVLDSNHTHEH
VLEELRLYAPLVSVGSYCVVMDTVVEDMPQDAFPDRPWGKGDNPKTAVWAYLKESSDFEIDQAIHSKLLITVAPDGYLRR
VR
>Mature_242_residues
MNPHEQFREEVKANIEGLQQDKALQTESLDWVGTTAKHKYTYNFSWMGRPIIQFPQDMVAMQEIIWNLRPDIIVETGIAH
GGSLVFYASILELIGHGEVLGIDIDIRQHNREAIEAHPMFKRISMIQGSSIDTAIVDQVRERVQGKKVLVVLDSNHTHEH
VLEELRLYAPLVSVGSYCVVMDTVVEDMPQDAFPDRPWGKGDNPKTAVWAYLKESSDFEIDQAIHSKLLITVAPDGYLRR
VR

Specific function: Catalyzes the O-methylation of the hydroxyl group located on C-2 of the first rhamnosyl residue linked to the phenolic group of glycosylated phenolphthiocerol dimycocerosates (PGL) and p-hydroxybenzoic acid derivatives (p-HBAD) [H]

COG id: COG3510

COG function: function code V; Cephalosporin hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rhamnosyl O-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007072 [H]

Pfam domain/function: PF04989 CmcI [H]

EC number: NA

Molecular weight: Translated: 27559; Mature: 27559

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPHEQFREEVKANIEGLQQDKALQTESLDWVGTTAKHKYTYNFSWMGRPIIQFPQDMVA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHCCHHHHH
MQEIIWNLRPDIIVETGIAHGGSLVFYASILELIGHGEVLGIDIDIRQHNREAIEAHPMF
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
KRISMIQGSSIDTAIVDQVRERVQGKKVLVVLDSNHTHEHVLEELRLYAPLVSVGSYCVV
HHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHH
MDTVVEDMPQDAFPDRPWGKGDNPKTAVWAYLKESSDFEIDQAIHSKLLITVAPDGYLRR
HHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEECCCCCCHHHHHHHCEEEEEECCCHHHCC
VR
CC
>Mature Secondary Structure
MNPHEQFREEVKANIEGLQQDKALQTESLDWVGTTAKHKYTYNFSWMGRPIIQFPQDMVA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCHHHHCCHHHHH
MQEIIWNLRPDIIVETGIAHGGSLVFYASILELIGHGEVLGIDIDIRQHNREAIEAHPMF
HHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
KRISMIQGSSIDTAIVDQVRERVQGKKVLVVLDSNHTHEHVLEELRLYAPLVSVGSYCVV
HHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHH
MDTVVEDMPQDAFPDRPWGKGDNPKTAVWAYLKESSDFEIDQAIHSKLLITVAPDGYLRR
HHHHHHHCCCCCCCCCCCCCCCCCCHHEEEEECCCCCCHHHHHHHCEEEEEECCCHHHCC
VR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]