Click here to switch to the map view.

The map label for this gene is opuE [H]

Identifier: 77457681

GI number: 77457681

Start: 1625040

End: 1626419

Strand: Reverse

Name: opuE [H]

Synonym: Pfl01_1454

Alternate gene names: 77457681

Gene position: 1626419-1625040 (Counterclockwise)

Preceding gene: 77457682

Following gene: 77457680

Centisome position: 25.26

GC content: 62.25

Gene sequence:

>1380_bases
ATGGCTTTGGATTTATTCGTCGTCCTCATCTACGCCGCCGGCATGCTCTTGCTCGGCTACTTCGGCATGCGCAAGGCCAA
GACCAACGAGGACTTCCTGGTCGCCGGGCGTAACCTCGGCCCTAGCCTGTACATGGGCACCATGGCTGCGACCGTTCTCG
GTGGCGCGTCCACCGTCGGCACCGTGCGTCTGGGCTACGTGCATGGCATCTCCGGTTTCTGGCTGTGCGCGGCACTGGGT
TGCGGCATCGTGGCGCTGAACCTGTTCCTGGCCAAGCCGCTGCTGAAACTGAAGATCTACACCGTTACCCAGGTCCTGGA
AAAACGCTACAACCCGATGGCCCGCTCGGCGAGCGCGGCGATCATGCTGGCCTACGCGCTGATGATCGGCGTGACCTCGA
TCCTCGCCATCGGCACCGTTCTTCAGGTTCTTTTCGGCCTGCCGTTCTGGATCTCGGTACTGCTCGGCGGTGGCGTGGTC
GTGGTGTATTCGGCCATCGGCGGCATGTGGTCGCTGACCCTGACCGACATCGTCCAGTTCATCATCAAGACCGTCGGCCT
GATGTTCATCCTGTTGCCAATCTGCCTGTACCGCGTCGGCGGCTGGGATGAACTGGTGCTGAAACTGCCGGCCACCGCCT
TCAGCTTCACCACCATCGGCTGGGACACGATCATCACCTACTTCATGATCTACTTCTTCGGCATCCTGATCGGTCAGGAC
ATCTGGCAACGGGTGTTCACCGTCAAGAACGAAAAAGTCGCTCAGTACGCCGGTAGCTTCGCGGGCATCTACTGCATCCT
TTACGGTCTGGCCTGTGCCCTGATCGGCATGGCAGCGCACGTTCTGATCCCGGATCTGGACAACGTCAACAACGCCTTCG
CCGCGATCGTCAAACTGTCGCTGCCGGACGGCATCCGTGGTCTGGTGATCGCCGCTGCCCTGGCCGCGATGATGTCCACC
GCCAGCGCCGGCCTGCTCGCCGCCGCCACCACCCTGACCGAAGACCTGCTGCCGAAACTGCGCGGCGGCAAACAGTCGAG
CCTGGGGGTGAACCGTCTGTTCACCCTGCTGACCGGTGTGGTGGTGCTGGGCATCGCGCTGGTGGTCAACGACGTGATCA
GCGCCCTGACCCTCGCTTACAACCTGCTGGTGGGCGGCATGCTGATACCGCTGATCGGGGCGATCTTCTGGAAACGCGCG
ACCACCGCCGGCGCCATCGCCAGCATGGGCCTGGGTTTCGCCACCGCCCTGCTGTTCATGTTCAAGGACGGTCTGGATGC
CAACACCCCGATCTACTACAGCCTGGCTGTGGGTCTGGTGAGTTTTGTGGTGGTCAGCCTGATGTCGCGTCGCCCAAACG
TGGTGGCCAGCGCGATCTAA

Upstream 100 bases:

>100_bases
GGCGCTGCGTCAGGCTGCCTGATAAACGAATCGCCTGTGGGGGTCAGCCTTCTCCCACAGTGTTCCACCCCATAAAAAAG
ACAATCGGAGACACGCCATC

Downstream 100 bases:

>100_bases
GCTGAACAAAACGACTTGATGCTTTCTTCGGCGGGTGTGGCGTCGGTTGCCACACCCGTTTTTTTCGTCTGGAGAAAATG
CTTGATGAAGATTGTTTCTC

Product: Na+/solute symporter

Products: Na (I) [Cytoplasm]; pantothenate [Cytoplasm] [C]

Alternate protein names: Sodium/proline symporter [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MALDLFVVLIYAAGMLLLGYFGMRKAKTNEDFLVAGRNLGPSLYMGTMAATVLGGASTVGTVRLGYVHGISGFWLCAALG
CGIVALNLFLAKPLLKLKIYTVTQVLEKRYNPMARSASAAIMLAYALMIGVTSILAIGTVLQVLFGLPFWISVLLGGGVV
VVYSAIGGMWSLTLTDIVQFIIKTVGLMFILLPICLYRVGGWDELVLKLPATAFSFTTIGWDTIITYFMIYFFGILIGQD
IWQRVFTVKNEKVAQYAGSFAGIYCILYGLACALIGMAAHVLIPDLDNVNNAFAAIVKLSLPDGIRGLVIAAALAAMMST
ASAGLLAAATTLTEDLLPKLRGGKQSSLGVNRLFTLLTGVVVLGIALVVNDVISALTLAYNLLVGGMLIPLIGAIFWKRA
TTAGAIASMGLGFATALLFMFKDGLDANTPIYYSLAVGLVSFVVVSLMSRRPNVVASAI

Sequences:

>Translated_459_residues
MALDLFVVLIYAAGMLLLGYFGMRKAKTNEDFLVAGRNLGPSLYMGTMAATVLGGASTVGTVRLGYVHGISGFWLCAALG
CGIVALNLFLAKPLLKLKIYTVTQVLEKRYNPMARSASAAIMLAYALMIGVTSILAIGTVLQVLFGLPFWISVLLGGGVV
VVYSAIGGMWSLTLTDIVQFIIKTVGLMFILLPICLYRVGGWDELVLKLPATAFSFTTIGWDTIITYFMIYFFGILIGQD
IWQRVFTVKNEKVAQYAGSFAGIYCILYGLACALIGMAAHVLIPDLDNVNNAFAAIVKLSLPDGIRGLVIAAALAAMMST
ASAGLLAAATTLTEDLLPKLRGGKQSSLGVNRLFTLLTGVVVLGIALVVNDVISALTLAYNLLVGGMLIPLIGAIFWKRA
TTAGAIASMGLGFATALLFMFKDGLDANTPIYYSLAVGLVSFVVVSLMSRRPNVVASAI
>Mature_458_residues
ALDLFVVLIYAAGMLLLGYFGMRKAKTNEDFLVAGRNLGPSLYMGTMAATVLGGASTVGTVRLGYVHGISGFWLCAALGC
GIVALNLFLAKPLLKLKIYTVTQVLEKRYNPMARSASAAIMLAYALMIGVTSILAIGTVLQVLFGLPFWISVLLGGGVVV
VYSAIGGMWSLTLTDIVQFIIKTVGLMFILLPICLYRVGGWDELVLKLPATAFSFTTIGWDTIITYFMIYFFGILIGQDI
WQRVFTVKNEKVAQYAGSFAGIYCILYGLACALIGMAAHVLIPDLDNVNNAFAAIVKLSLPDGIRGLVIAAALAAMMSTA
SAGLLAAATTLTEDLLPKLRGGKQSSLGVNRLFTLLTGVVVLGIALVVNDVISALTLAYNLLVGGMLIPLIGAIFWKRAT
TAGAIASMGLGFATALLFMFKDGLDANTPIYYSLAVGLVSFVVVSLMSRRPNVVASAI

Specific function: Catalyzes the sodium-dependent uptake of extracellular proline [H]

COG id: COG0591

COG function: function code ER; Na+/proline symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family [H]

Homologues:

Organism=Homo sapiens, GI310128183, Length=478, Percent_Identity=24.2677824267782, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI4507035, Length=397, Percent_Identity=24.4332493702771, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI157671931, Length=483, Percent_Identity=25.879917184265, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI87082237, Length=420, Percent_Identity=26.4285714285714, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787251, Length=470, Percent_Identity=24.2553191489362, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17539284, Length=462, Percent_Identity=24.4588744588745, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648033, Length=424, Percent_Identity=23.5849056603774, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21356865, Length=424, Percent_Identity=23.5849056603774, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011851
- InterPro:   IPR001734
- InterPro:   IPR018212
- InterPro:   IPR019900 [H]

Pfam domain/function: PF00474 SSF [H]

EC number: NA

Molecular weight: Translated: 48623; Mature: 48492

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50283 NA_SOLUT_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
ALDLFVVLIYAAGMLLLGYFGMRKAKTNEDFLVAGRNLGPSLYMGTMAATVLGGASTVG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHH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HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; pantothenate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + pantothenate [Periplasm] = Na (I) [Cytoplasm] + pantothenate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377 [H]