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The map label for this gene is pleD [H]

Identifier: 77457563

GI number: 77457563

Start: 1508779

End: 1510266

Strand: Reverse

Name: pleD [H]

Synonym: Pfl01_1336

Alternate gene names: 77457563

Gene position: 1510266-1508779 (Counterclockwise)

Preceding gene: 77457564

Following gene: 77457562

Centisome position: 23.46

GC content: 59.01

Gene sequence:

>1488_bases
ATGCCGCTCAGATCTGCGTTTCACTCCCAACGTTCGCTGGTACTGACCCTCGTCGCCCTGCTCGGCGCAGGTTTCCTTGC
TACTTCGCTGCTCAGCTACTACGCCTCGCGGGCGTCGATCCGCGACAACATCGTCAACACCGAACTGCCGCTGACCTCCG
ACACGGTGTACTCGGAAATCCAGAAAGACCTGGTCAGACCGATCCTTATTTCTTCGATGATGTCCCGCGACACCTTCATG
CGCGACTGGGTAGTGAACGGCGAGAAAGACAGCGACCAGATGACCCGCTACCTCGACGAGGTCATGACCCACTACGGCGC
CTACACAGCGTTTTTCGTCTCCAACAGCACCCACACTTACTACCACGCCAAAGGTGTGCTCAAGCAGGTAAAGATCGACG
AACCGCGTGACGCCTGGTACTTCCGCGTACGCGACATGAAGGATCCATACGAGATCAATGTCGACCCGGATCTGGCCAAC
AAAGACAACATGACGTTCTTCATCAACTACAAGGTGTACGACTACAACAATCGCTTCATCGGCGCCGCTGGCGTGGGGCT
GACGGTGGACGCGGTGATCAAGCTGATCGACAAGTATCAGCAGCGCTATCAACGCAGCGTTTACTTCGTCGACACCTTCG
GTCGGCTGGTCTTGACCGGCGCCGAGGGTGGGCCGGAAGGGGCGCGGGTCGGGAAAAGCCTGACCGACCTCGACAGCATG
AAAAGCCTGGTCAGCCAATTGCCCAAACCCCACAGCGGCAGCTACGAATATTCCACCCACGGCCAGGGGCATTTCCTCAA
CGTGCGGTTCATTCCGGAGCTGAACTGGTACCTGTTTGTCGACAAACGCGAAGACGGCGCACTGAGCGAAATCCGCCAGT
CGCTCTATCTCAACCTGCTGATCTGCCTGCTGGTGACGCTGATCGTGCTGGCCCTGGTCAACCGCTTGATCCGCCGCTAT
CAGGAAAAAATCCACGCCCAGGCCACCCTCGACAGCCTCACCGAACTGCCCAACCGCCGCGGCTTCGACCTGCTCGCCGC
GCAAGCGCTGCACGAAGCGCACCGTGAGCCGAAACCGCTGACCGCGCTGCTGCTGGATCTGGATCACTTCAAGGTTTTGA
ACGACACCTACGGGCACATGGCCGGCGATCAGGTGCTGATCGGTTTCGCCAGGGACCTGCAAAGTTGTCTGCGGCATTCC
GACATTGTCTGTCGTTGGGGCGGTGAGGAATTTATCGTGCTGCTGAAGGACACCGACGGCGAGACCGGTCAGAAGATTGC
CGAGAAGATCCGCCAGCACGTCGAACAGCATGAATATGCCTACAACGGTCACACCCTGAATCTGACCGTCAGCATCGGCG
CCACCACCCTGCAACCGGATGACACCTTGCACAGTCTGCTGTCCCGGGCCGATCATGCGATGTACCGGGCCAAACAGACC
GGCCGCAACCGCACCTGCGTGGAAATGCCTCACTCGACCTATGCCTGA

Upstream 100 bases:

>100_bases
CCCGGCTGATCCCCCCGCATTTCAGGCACAAATCCCGCAGTCTTTTCCCCGCCGTGCCACTAGAATGCTGCCCATCGGGT
CACTGTCAACGGAATTGCCA

Downstream 100 bases:

>100_bases
CACCAATAACACACCTGACCTCTGCCCGGCCTGCGGCGCCCGCAACGACTGCACCCTGGCCGACCCGCGCACCGCCGACC
GCGCCTGCTGGTGCTACGGC

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MPLRSAFHSQRSLVLTLVALLGAGFLATSLLSYYASRASIRDNIVNTELPLTSDTVYSEIQKDLVRPILISSMMSRDTFM
RDWVVNGEKDSDQMTRYLDEVMTHYGAYTAFFVSNSTHTYYHAKGVLKQVKIDEPRDAWYFRVRDMKDPYEINVDPDLAN
KDNMTFFINYKVYDYNNRFIGAAGVGLTVDAVIKLIDKYQQRYQRSVYFVDTFGRLVLTGAEGGPEGARVGKSLTDLDSM
KSLVSQLPKPHSGSYEYSTHGQGHFLNVRFIPELNWYLFVDKREDGALSEIRQSLYLNLLICLLVTLIVLALVNRLIRRY
QEKIHAQATLDSLTELPNRRGFDLLAAQALHEAHREPKPLTALLLDLDHFKVLNDTYGHMAGDQVLIGFARDLQSCLRHS
DIVCRWGGEEFIVLLKDTDGETGQKIAEKIRQHVEQHEYAYNGHTLNLTVSIGATTLQPDDTLHSLLSRADHAMYRAKQT
GRNRTCVEMPHSTYA

Sequences:

>Translated_495_residues
MPLRSAFHSQRSLVLTLVALLGAGFLATSLLSYYASRASIRDNIVNTELPLTSDTVYSEIQKDLVRPILISSMMSRDTFM
RDWVVNGEKDSDQMTRYLDEVMTHYGAYTAFFVSNSTHTYYHAKGVLKQVKIDEPRDAWYFRVRDMKDPYEINVDPDLAN
KDNMTFFINYKVYDYNNRFIGAAGVGLTVDAVIKLIDKYQQRYQRSVYFVDTFGRLVLTGAEGGPEGARVGKSLTDLDSM
KSLVSQLPKPHSGSYEYSTHGQGHFLNVRFIPELNWYLFVDKREDGALSEIRQSLYLNLLICLLVTLIVLALVNRLIRRY
QEKIHAQATLDSLTELPNRRGFDLLAAQALHEAHREPKPLTALLLDLDHFKVLNDTYGHMAGDQVLIGFARDLQSCLRHS
DIVCRWGGEEFIVLLKDTDGETGQKIAEKIRQHVEQHEYAYNGHTLNLTVSIGATTLQPDDTLHSLLSRADHAMYRAKQT
GRNRTCVEMPHSTYA
>Mature_494_residues
PLRSAFHSQRSLVLTLVALLGAGFLATSLLSYYASRASIRDNIVNTELPLTSDTVYSEIQKDLVRPILISSMMSRDTFMR
DWVVNGEKDSDQMTRYLDEVMTHYGAYTAFFVSNSTHTYYHAKGVLKQVKIDEPRDAWYFRVRDMKDPYEINVDPDLANK
DNMTFFINYKVYDYNNRFIGAAGVGLTVDAVIKLIDKYQQRYQRSVYFVDTFGRLVLTGAEGGPEGARVGKSLTDLDSMK
SLVSQLPKPHSGSYEYSTHGQGHFLNVRFIPELNWYLFVDKREDGALSEIRQSLYLNLLICLLVTLIVLALVNRLIRRYQ
EKIHAQATLDSLTELPNRRGFDLLAAQALHEAHREPKPLTALLLDLDHFKVLNDTYGHMAGDQVLIGFARDLQSCLRHSD
IVCRWGGEEFIVLLKDTDGETGQKIAEKIRQHVEQHEYAYNGHTLNLTVSIGATTLQPDDTLHSLLSRADHAMYRAKQTG
RNRTCVEMPHSTYA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=198, Percent_Identity=37.3737373737374, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1787816, Length=159, Percent_Identity=41.5094339622642, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI87081881, Length=167, Percent_Identity=40.1197604790419, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693134, Length=194, Percent_Identity=34.5360824742268, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=38.6503067484663, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1786584, Length=170, Percent_Identity=34.1176470588235, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=199, Percent_Identity=33.6683417085427, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1787541, Length=176, Percent_Identity=34.6590909090909, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=33.6898395721925, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87081974, Length=196, Percent_Identity=28.0612244897959, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI87081977, Length=187, Percent_Identity=33.1550802139037, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=151, Percent_Identity=37.0860927152318, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=160, Percent_Identity=31.875, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56363; Mature: 56232

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRSAFHSQRSLVLTLVALLGAGFLATSLLSYYASRASIRDNIVNTELPLTSDTVYSEI
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
QKDLVRPILISSMMSRDTFMRDWVVNGEKDSDQMTRYLDEVMTHYGAYTAFFVSNSTHTY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEE
YHAKGVLKQVKIDEPRDAWYFRVRDMKDPYEINVDPDLANKDNMTFFINYKVYDYNNRFI
EEHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEEEEECCEEE
GAAGVGLTVDAVIKLIDKYQQRYQRSVYFVDTFGRLVLTGAEGGPEGARVGKSLTDLDSM
ECCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCHHHHCCHHHHHHHH
KSLVSQLPKPHSGSYEYSTHGQGHFLNVRFIPELNWYLFVDKREDGALSEIRQSLYLNLL
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
ICLLVTLIVLALVNRLIRRYQEKIHAQATLDSLTELPNRRGFDLLAAQALHEAHREPKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCH
TALLLDLDHFKVLNDTYGHMAGDQVLIGFARDLQSCLRHSDIVCRWGGEEFIVLLKDTDG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCC
ETGQKIAEKIRQHVEQHEYAYNGHTLNLTVSIGATTLQPDDTLHSLLSRADHAMYRAKQT
CHHHHHHHHHHHHHHHHCEEECCEEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHC
GRNRTCVEMPHSTYA
CCCCCEEECCCCCCC
>Mature Secondary Structure 
PLRSAFHSQRSLVLTLVALLGAGFLATSLLSYYASRASIRDNIVNTELPLTSDTVYSEI
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
QKDLVRPILISSMMSRDTFMRDWVVNGEKDSDQMTRYLDEVMTHYGAYTAFFVSNSTHTY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCEE
YHAKGVLKQVKIDEPRDAWYFRVRDMKDPYEINVDPDLANKDNMTFFINYKVYDYNNRFI
EEHHHHHHHCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEEEEECCEEE
GAAGVGLTVDAVIKLIDKYQQRYQRSVYFVDTFGRLVLTGAEGGPEGARVGKSLTDLDSM
ECCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCCCCHHHHCCHHHHHHHH
KSLVSQLPKPHSGSYEYSTHGQGHFLNVRFIPELNWYLFVDKREDGALSEIRQSLYLNLL
HHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHH
ICLLVTLIVLALVNRLIRRYQEKIHAQATLDSLTELPNRRGFDLLAAQALHEAHREPKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCH
TALLLDLDHFKVLNDTYGHMAGDQVLIGFARDLQSCLRHSDIVCRWGGEEFIVLLKDTDG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCC
ETGQKIAEKIRQHVEQHEYAYNGHTLNLTVSIGATTLQPDDTLHSLLSRADHAMYRAKQT
CHHHHHHHHHHHHHHHHCEEECCEEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHC
GRNRTCVEMPHSTYA
CCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA