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The map label for this gene is gatA [H]

Identifier: 77457564

GI number: 77457564

Start: 1510435

End: 1512207

Strand: Reverse

Name: gatA [H]

Synonym: Pfl01_1337

Alternate gene names: 77457564

Gene position: 1512207-1510435 (Counterclockwise)

Preceding gene: 77457565

Following gene: 77457563

Centisome position: 23.49

GC content: 65.09

Gene sequence:

>1773_bases
ATGAATCTGCAACTCGATGCCCTGCGCCGGGCCTACCGCAATGGCGACACCACACCCCGGCAATTGCTGTTGGCGTTGCG
GGAAAAAGCCGCCGCGCTGAACCCGGACTATCACCTGTTCATCCATCTGCTCAGCGCCGAAGAACTGGAACCGTATCTGG
CCGCCCTCGACGGTCGCGATCTCGACAGCCTGCCGTTGTATGGCGTGCCGTTCGCGATCAAGGACAACATTGATCTGGCC
GGTGTCCCGACCACCGCTGCATGTCCGGCGTTCGCCTACGTACCGGAACGTTCGGCGACCATCGTCGAGCAACTGCTGGC
GCTGGGCGCGATCCCGCTGGGCAAGACCAATCTCGACCAGTTCGCCACCGGTCTCAACGGCAGCCGCTCGCCGTACGGCG
CCTGCCCGAATAGCGTGATGGCGGAATATCCGTCCGGCGGCTCAAGCGCCGGCTCATCGCTGGCCGTGGCGCTCGGCGTG
GCCAGTTTCGCGTTGGGCACCGACACTGCCGGCTCCGGCCGGGTGCCGGCGGCGTTGAACAATCTGGTGGGCCTGAAGGC
CAGCAAAGGGCTGATCTCCACCGCCGGCGTGCTGCCGGCCTGTCGCACGCTGGACTGCGTCACCACGTTCACCGCAACGG
CCCGGGAAGCCAGTCAGCTGCTGGCGCTGACCGCGCGCCTCGATCCGAGGGACGAATACAGCCGCAGCAATCCGTCATGG
AACGACGGTACCGCGTTCGGCGCGCCACGGCCGTTTCGCTTCGGTGTACCGCGTGCGCGAGACCTGGCGTTTTTCGGCTG
TGAGGAAGGCCCGCGGCTGTTCGGCGATGCCATCGACCGTCTGAAAGCTCTGGGCGGTGAAGCGGTCGAGCTGGACTTGT
CGCCGTTCCTCGAAGCGGCGCGGTTGCTCTATGAAGGGCCGTGGGTGGCCGAGCGCTACAGCGTGGCCGGCGAGTTGATG
GAAGAAAATCCCGAAGCGGTGCTGCCGGTGATCCGCGCGGTGCTGGCCAAAGCACCGGCCGTCAGTGGCGTGCAGACGTT
CCGCGCGCAATACCGCCTGCAAGCGCTGAAAGCCGTTTGCGATAAAGCACTGGAAGAACTCGACTGCGTGCTCACGCCCA
CCATCGGTCGTCCGGTGACGCTGGCGGAACTGGACGCCGAACCGGTGCTGCGCAATTCCGAACTGGGCTACTACACCAAC
TTCATGAACCTGCTCGATTACGCTGCCGTCGCCGTGCCCAGCGGTTTCATGGCCAACGGGCTGCCGTGGGGTGTGACGCT
GTTCGGACGAACGTTCACCGACCAGTATTTGCTGAGTGTGGCGGACGCTTTGCAGCGCCAGCAGGATCCCGCGCTGCCGA
CGCCGAACAACCCGGCGCGCCATGACCGTGCACGACTGGTGGTCTGCGGTGCGCACCTGGACGGACTGGCCCAGAACTGG
CAGTTGCGGCAACGCGGCGCACGCCTGATCGAGGCCACTTACAGCTCGAAGAACTACAAACTCTATGCCTTGGCCGGCGG
CCCACCGTTGCGTCCAGGAATGCTGCGCGTGGCCGAAGGTGGCGTGGCCATTGCAGTGGAAGTCTGGGAGTTGCCGAGCA
GTGAACTGGGCTCGTTCCTGACCGGCATCCCGGCACCGCTGGGACTGGGCAAGGTGCAACTGGCGGATGGCCGTTGGGAA
AGCGGATTCATCTGCGAACCCTACGGCCTGGAAGGTGCGATCGACATCAGCCACCTGGGCGGGTGGCGAGCGTATCTGCA
AACCGGAAACTGA

Upstream 100 bases:

>100_bases
GGTGCAGGACATTCGCGTGCAGCCGGGATCGGCGGTGCGTGCCGGACAACGGGTCGTGGTGCTGGAACTCGACTGAAACC
ATTTGCCAAAGGATCAACCC

Downstream 100 bases:

>100_bases
TCCGAACGGCGGCCCCCTTTCGTTCCGGGAGGGGGCTGGATGAGTGAGCCATTCTCCAGACACCCCTGCCCGGCTGATCC
CCCCGCATTTCAGGCACAAA

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MNLQLDALRRAYRNGDTTPRQLLLALREKAAALNPDYHLFIHLLSAEELEPYLAALDGRDLDSLPLYGVPFAIKDNIDLA
GVPTTAACPAFAYVPERSATIVEQLLALGAIPLGKTNLDQFATGLNGSRSPYGACPNSVMAEYPSGGSSAGSSLAVALGV
ASFALGTDTAGSGRVPAALNNLVGLKASKGLISTAGVLPACRTLDCVTTFTATAREASQLLALTARLDPRDEYSRSNPSW
NDGTAFGAPRPFRFGVPRARDLAFFGCEEGPRLFGDAIDRLKALGGEAVELDLSPFLEAARLLYEGPWVAERYSVAGELM
EENPEAVLPVIRAVLAKAPAVSGVQTFRAQYRLQALKAVCDKALEELDCVLTPTIGRPVTLAELDAEPVLRNSELGYYTN
FMNLLDYAAVAVPSGFMANGLPWGVTLFGRTFTDQYLLSVADALQRQQDPALPTPNNPARHDRARLVVCGAHLDGLAQNW
QLRQRGARLIEATYSSKNYKLYALAGGPPLRPGMLRVAEGGVAIAVEVWELPSSELGSFLTGIPAPLGLGKVQLADGRWE
SGFICEPYGLEGAIDISHLGGWRAYLQTGN

Sequences:

>Translated_590_residues
MNLQLDALRRAYRNGDTTPRQLLLALREKAAALNPDYHLFIHLLSAEELEPYLAALDGRDLDSLPLYGVPFAIKDNIDLA
GVPTTAACPAFAYVPERSATIVEQLLALGAIPLGKTNLDQFATGLNGSRSPYGACPNSVMAEYPSGGSSAGSSLAVALGV
ASFALGTDTAGSGRVPAALNNLVGLKASKGLISTAGVLPACRTLDCVTTFTATAREASQLLALTARLDPRDEYSRSNPSW
NDGTAFGAPRPFRFGVPRARDLAFFGCEEGPRLFGDAIDRLKALGGEAVELDLSPFLEAARLLYEGPWVAERYSVAGELM
EENPEAVLPVIRAVLAKAPAVSGVQTFRAQYRLQALKAVCDKALEELDCVLTPTIGRPVTLAELDAEPVLRNSELGYYTN
FMNLLDYAAVAVPSGFMANGLPWGVTLFGRTFTDQYLLSVADALQRQQDPALPTPNNPARHDRARLVVCGAHLDGLAQNW
QLRQRGARLIEATYSSKNYKLYALAGGPPLRPGMLRVAEGGVAIAVEVWELPSSELGSFLTGIPAPLGLGKVQLADGRWE
SGFICEPYGLEGAIDISHLGGWRAYLQTGN
>Mature_590_residues
MNLQLDALRRAYRNGDTTPRQLLLALREKAAALNPDYHLFIHLLSAEELEPYLAALDGRDLDSLPLYGVPFAIKDNIDLA
GVPTTAACPAFAYVPERSATIVEQLLALGAIPLGKTNLDQFATGLNGSRSPYGACPNSVMAEYPSGGSSAGSSLAVALGV
ASFALGTDTAGSGRVPAALNNLVGLKASKGLISTAGVLPACRTLDCVTTFTATAREASQLLALTARLDPRDEYSRSNPSW
NDGTAFGAPRPFRFGVPRARDLAFFGCEEGPRLFGDAIDRLKALGGEAVELDLSPFLEAARLLYEGPWVAERYSVAGELM
EENPEAVLPVIRAVLAKAPAVSGVQTFRAQYRLQALKAVCDKALEELDCVLTPTIGRPVTLAELDAEPVLRNSELGYYTN
FMNLLDYAAVAVPSGFMANGLPWGVTLFGRTFTDQYLLSVADALQRQQDPALPTPNNPARHDRARLVVCGAHLDGLAQNW
QLRQRGARLIEATYSSKNYKLYALAGGPPLRPGMLRVAEGGVAIAVEVWELPSSELGSFLTGIPAPLGLGKVQLADGRWE
SGFICEPYGLEGAIDISHLGGWRAYLQTGN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=27.1084337349398, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI195972892, Length=199, Percent_Identity=27.6381909547739, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=28.921568627451, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71990152, Length=190, Percent_Identity=32.6315789473684, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=126, Percent_Identity=34.1269841269841, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=162, Percent_Identity=32.0987654320988, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=136, Percent_Identity=33.0882352941176, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=579, Percent_Identity=42.4870466321244, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6323950, Length=273, Percent_Identity=28.2051282051282, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648113, Length=435, Percent_Identity=27.1264367816092, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24644968, Length=482, Percent_Identity=21.7842323651452, Blast_Score=69, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24652985, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI19922090, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24652981, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24652983, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 63000; Mature: 63000

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLQLDALRRAYRNGDTTPRQLLLALREKAAALNPDYHLFIHLLSAEELEPYLAALDGRD
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCHHHHHCCCCC
LDSLPLYGVPFAIKDNIDLAGVPTTAACPAFAYVPERSATIVEQLLALGAIPLGKTNLDQ
CCCCCCCCCCEEEECCCCCCCCCCHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCHHH
FATGLNGSRSPYGACPNSVMAEYPSGGSSAGSSLAVALGVASFALGTDTAGSGRVPAALN
HHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
NLVGLKASKGLISTAGVLPACRTLDCVTTFTATAREASQLLALTARLDPRDEYSRSNPSW
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC
NDGTAFGAPRPFRFGVPRARDLAFFGCEEGPRLFGDAIDRLKALGGEAVELDLSPFLEAA
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHH
RLLYEGPWVAERYSVAGELMEENPEAVLPVIRAVLAKAPAVSGVQTFRAQYRLQALKAVC
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DKALEELDCVLTPTIGRPVTLAELDAEPVLRNSELGYYTNFMNLLDYAAVAVPSGFMANG
HHHHHHCCEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCC
LPWGVTLFGRTFTDQYLLSVADALQRQQDPALPTPNNPARHDRARLVVCGAHLDGLAQNW
CCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHH
QLRQRGARLIEATYSSKNYKLYALAGGPPLRPGMLRVAEGGVAIAVEVWELPSSELGSFL
HHHHHHHHEEHHEECCCCEEEEEEECCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHH
TGIPAPLGLGKVQLADGRWESGFICEPYGLEGAIDISHLGGWRAYLQTGN
HCCCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEHHHCCCEEEEEECCC
>Mature Secondary Structure
MNLQLDALRRAYRNGDTTPRQLLLALREKAAALNPDYHLFIHLLSAEELEPYLAALDGRD
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCHHHHHCCCCC
LDSLPLYGVPFAIKDNIDLAGVPTTAACPAFAYVPERSATIVEQLLALGAIPLGKTNLDQ
CCCCCCCCCCEEEECCCCCCCCCCHHCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCHHH
FATGLNGSRSPYGACPNSVMAEYPSGGSSAGSSLAVALGVASFALGTDTAGSGRVPAALN
HHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
NLVGLKASKGLISTAGVLPACRTLDCVTTFTATAREASQLLALTARLDPRDEYSRSNPSW
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC
NDGTAFGAPRPFRFGVPRARDLAFFGCEEGPRLFGDAIDRLKALGGEAVELDLSPFLEAA
CCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHH
RLLYEGPWVAERYSVAGELMEENPEAVLPVIRAVLAKAPAVSGVQTFRAQYRLQALKAVC
HHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
DKALEELDCVLTPTIGRPVTLAELDAEPVLRNSELGYYTNFMNLLDYAAVAVPSGFMANG
HHHHHHCCEEECCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCC
LPWGVTLFGRTFTDQYLLSVADALQRQQDPALPTPNNPARHDRARLVVCGAHLDGLAQNW
CCCCEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHH
QLRQRGARLIEATYSSKNYKLYALAGGPPLRPGMLRVAEGGVAIAVEVWELPSSELGSFL
HHHHHHHHEEHHEECCCCEEEEEEECCCCCCCCCEEEECCCEEEEEEEECCCCHHHHHHH
TGIPAPLGLGKVQLADGRWESGFICEPYGLEGAIDISHLGGWRAYLQTGN
HCCCCCCCCCEEEEECCCCCCCEEECCCCCCCEEEHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]