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The map label for this gene is rsuA [H]

Identifier: 77457562

GI number: 77457562

Start: 1507830

End: 1508522

Strand: Reverse

Name: rsuA [H]

Synonym: Pfl01_1335

Alternate gene names: 77457562

Gene position: 1508522-1507830 (Counterclockwise)

Preceding gene: 77457563

Following gene: 77457561

Centisome position: 23.43

GC content: 61.18

Gene sequence:

>693_bases
ATGCGTGTCGACCGTTTCCTGAGTAACCTGCCCCGCTACAACCGCAAGCAGGTTCGCCTGTTGCTGGTGGAAAAGCGCGT
GCGCATTGACGGAAAAGTCGTCAGCGATCCCCACAGCGAAGTCCTGGAGTTCAGCCGCGTGGAGGTCGACGACGAAGTGC
TGCAACCCGGCAAACCGGCGCGCTACTTCATGCTGAACAAACCGCAGGGCTGCGTCAGCGCCACCCGCGATCCCGAGCAT
CCGACCGTGCTCGACCTGATTCACGAGCCGGACAAGGACGACCTGCACATCGCCGGGCGTCTGGATTTCAACACCACCGG
GCTGATGCTGATCACCAACGACGGCAGCTGGTCGCGGCGCCTGACCCAGCCGCAGACCAAACTGCCGAAGGTCTATTACG
TCGAGACCGAACAGGAGATCGGCCCGGAATATGCGATCAAGTTCGCCGAGGGGATCTACTTCGCCTTTGAGGACCTGACC
ACCCAACCGGCGCAGCTGCAAGTGCTCGGCCCGCGAACGGCGCGGTTGAGCATCGTCGAAGGTCGTTACCATCAGGTGAA
GCGCATGTTCGGCCACTTCAACAACAAAGTGCTGCGCCTGCACCGCGAATCCATGGGCCCGCTGGCGCTGGATCACGCGC
TAAAACCGGGCGAGTACCGCGCTTTGCGCACCGAAGAGATTCATTTGTTCTAA

Upstream 100 bases:

>100_bases
CTGCCTGTGCCCGCGCTGCGCCCAGGTCGAGGCGCAACTGCAAGCGGCCAGGCCGTCGATCCCGTAAGATGCGCGCCCCG
CTACCTGCCGATTGCTGATC

Downstream 100 bases:

>100_bases
GCAGCCGACCGCTCAGCAGAAGTTGTCGAACAATTTACCAACGGCACTTGCGCGATCCGGATCCCCCTGCTTGAATCAGG
TCGTCGGCCAAATTGTGACC

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRVDRFLSNLPRYNRKQVRLLLVEKRVRIDGKVVSDPHSEVLEFSRVEVDDEVLQPGKPARYFMLNKPQGCVSATRDPEH
PTVLDLIHEPDKDDLHIAGRLDFNTTGLMLITNDGSWSRRLTQPQTKLPKVYYVETEQEIGPEYAIKFAEGIYFAFEDLT
TQPAQLQVLGPRTARLSIVEGRYHQVKRMFGHFNNKVLRLHRESMGPLALDHALKPGEYRALRTEEIHLF

Sequences:

>Translated_230_residues
MRVDRFLSNLPRYNRKQVRLLLVEKRVRIDGKVVSDPHSEVLEFSRVEVDDEVLQPGKPARYFMLNKPQGCVSATRDPEH
PTVLDLIHEPDKDDLHIAGRLDFNTTGLMLITNDGSWSRRLTQPQTKLPKVYYVETEQEIGPEYAIKFAEGIYFAFEDLT
TQPAQLQVLGPRTARLSIVEGRYHQVKRMFGHFNNKVLRLHRESMGPLALDHALKPGEYRALRTEEIHLF
>Mature_230_residues
MRVDRFLSNLPRYNRKQVRLLLVEKRVRIDGKVVSDPHSEVLEFSRVEVDDEVLQPGKPARYFMLNKPQGCVSATRDPEH
PTVLDLIHEPDKDDLHIAGRLDFNTTGLMLITNDGSWSRRLTQPQTKLPKVYYVETEQEIGPEYAIKFAEGIYFAFEDLT
TQPAQLQVLGPRTARLSIVEGRYHQVKRMFGHFNNKVLRLHRESMGPLALDHALKPGEYRALRTEEIHLF

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=227, Percent_Identity=45.374449339207, Blast_Score=192, Evalue=1e-50,
Organism=Escherichia coli, GI87081838, Length=181, Percent_Identity=30.3867403314917, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790453, Length=233, Percent_Identity=24.4635193133047, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=24.6753246753247, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26644; Mature: 26644

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDRFLSNLPRYNRKQVRLLLVEKRVRIDGKVVSDPHSEVLEFSRVEVDDEVLQPGKPA
CCHHHHHHHCCCCCCHHEEEEEEEHHHCCCCEEECCCHHHHHHHHHCCCCHHHHCCCCCC
RYFMLNKPQGCVSATRDPEHPTVLDLIHEPDKDDLHIAGRLDFNTTGLMLITNDGSWSRR
EEEEEECCCCCCCCCCCCCCCEEEHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCC
LTQPQTKLPKVYYVETEQEIGPEYAIKFAEGIYFAFEDLTTQPAQLQVLGPRTARLSIVE
CCCCHHHCCEEEEEECHHHCCHHHHHHHHCCEEEEECCCCCCCCEEEEECCCCEEEEEEC
GRYHQVKRMFGHFNNKVLRLHRESMGPLALDHALKPGEYRALRTEEIHLF
CHHHHHHHHHHHHCCCEEEEEHHCCCCEEHHHCCCCCCEEEEECEEEEEC
>Mature Secondary Structure
MRVDRFLSNLPRYNRKQVRLLLVEKRVRIDGKVVSDPHSEVLEFSRVEVDDEVLQPGKPA
CCHHHHHHHCCCCCCHHEEEEEEEHHHCCCCEEECCCHHHHHHHHHCCCCHHHHCCCCCC
RYFMLNKPQGCVSATRDPEHPTVLDLIHEPDKDDLHIAGRLDFNTTGLMLITNDGSWSRR
EEEEEECCCCCCCCCCCCCCCEEEHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCC
LTQPQTKLPKVYYVETEQEIGPEYAIKFAEGIYFAFEDLTTQPAQLQVLGPRTARLSIVE
CCCCHHHCCEEEEEECHHHCCHHHHHHHHCCEEEEECCCCCCCCEEEEECCCCEEEEEEC
GRYHQVKRMFGHFNNKVLRLHRESMGPLALDHALKPGEYRALRTEEIHLF
CHHHHHHHHHHHHCCCEEEEEHHCCCCEEHHHCCCCCCEEEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]