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The map label for this gene is nemA [H]

Identifier: 77457509

GI number: 77457509

Start: 1458476

End: 1459525

Strand: Reverse

Name: nemA [H]

Synonym: Pfl01_1282

Alternate gene names: 77457509

Gene position: 1459525-1458476 (Counterclockwise)

Preceding gene: 77457510

Following gene: 77457507

Centisome position: 22.67

GC content: 65.43

Gene sequence:

>1050_bases
ATGGCAACAATTTTCGATCCGATCAAACTGGGCGACCTCGAGCTGTCCAACCGCATCATCATGGCGCCGCTGACCCGTTG
CCGCGCCGACGAAGGCCGCGTACCGAACGCGCTGATGGCCGAGTACTACGTACAACGCGCCTCGGCCGGCCTGATCCTCA
GCGAAGCCACCTCGGTCACCCCGATGGGCGTCGGCTACCCGGACACCCCGGGCATCTGGTCCAACGACCAGGTGCGCGGC
TGGACCAACGTGACCAAGGCCGTTCACGCCGCCGGCGGCAAGATCGTCCTGCAACTGTGGCACGTCGGCCGGATCTCCCA
CCCGTCGTACCTGAACGGCGAAACGCCGGTTGCACCGAGCGCCATCCAGCCAAAAGGCCATGTCAGCCTGGTTCGCCCGC
TGGCCGACTATCCAACCCCGCGTGCGCTGGAAACCGCTGAAATCGCCGACATCGTCGACGCCTACCGCGTGGGTGCGGAA
AACGCCAAGGCGGCCGGTTTCGACGGCGTGGAAATCCACGGCGCCAACGGTTACCTGCTCGACCAGTTCCTGCAAAGCAG
CACCAACCAGCGCACCGACAACTACGGCGGCTCCCTGGAAAACCGTGCACGCCTGCTGCTGGAAGTGACCGACGCGGCCA
TCGAAGTCTGGGGCGCCGGCCGTGTCGGCGTACACCTGGCACCACGCGCCGACTCCCATGACATGGGCGACGACAACCTG
GCCGAAACCTTCACCTACGTCGCCCGCGAGCTGGGCAAGCGTGGCATCGCCTTCATCTGCTCCCGCGAGAAAGAAGGCGC
CGACAGCCTCGGTCCACAACTGAAAGAAGCCTTCGGCGGCGCGTACATCGCCAACGAGCGTTTCACCAAGGACAGCGCCA
ACGCGTGGCTGGCCGAGGGCAAGGCTGACGCCGTGGCATTCGGCATCCCGTTCATTGCCAACCCGGACCTGCCGGCCCGT
CTGAAGGCCGATGCGCCGCTGAACGAGCCGCACCCGGAAACCTTCTACGGCAAAGGTGCGGTCGGTTACCTCGACTACCC
GACCCTGTAA

Upstream 100 bases:

>100_bases
TCGCGCTGCTGGTGACCCTGATCACATTCCGCCAGAACGGCAATCCCGAGCTGGCCACCGCTAACTGATTCACTCACTTA
CCTCACGAGGGTTGTATCTC

Downstream 100 bases:

>100_bases
GCCACACCGCAGAAACACAAAAGCCCCCGACTCGTGAGAATCGGGGGCTTTTTATTGGTCGCCGGACGGGTGACTCGCTC
ACATTCCTTCACAGGCTCGA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MATIFDPIKLGDLELSNRIIMAPLTRCRADEGRVPNALMAEYYVQRASAGLILSEATSVTPMGVGYPDTPGIWSNDQVRG
WTNVTKAVHAAGGKIVLQLWHVGRISHPSYLNGETPVAPSAIQPKGHVSLVRPLADYPTPRALETAEIADIVDAYRVGAE
NAKAAGFDGVEIHGANGYLLDQFLQSSTNQRTDNYGGSLENRARLLLEVTDAAIEVWGAGRVGVHLAPRADSHDMGDDNL
AETFTYVARELGKRGIAFICSREKEGADSLGPQLKEAFGGAYIANERFTKDSANAWLAEGKADAVAFGIPFIANPDLPAR
LKADAPLNEPHPETFYGKGAVGYLDYPTL

Sequences:

>Translated_349_residues
MATIFDPIKLGDLELSNRIIMAPLTRCRADEGRVPNALMAEYYVQRASAGLILSEATSVTPMGVGYPDTPGIWSNDQVRG
WTNVTKAVHAAGGKIVLQLWHVGRISHPSYLNGETPVAPSAIQPKGHVSLVRPLADYPTPRALETAEIADIVDAYRVGAE
NAKAAGFDGVEIHGANGYLLDQFLQSSTNQRTDNYGGSLENRARLLLEVTDAAIEVWGAGRVGVHLAPRADSHDMGDDNL
AETFTYVARELGKRGIAFICSREKEGADSLGPQLKEAFGGAYIANERFTKDSANAWLAEGKADAVAFGIPFIANPDLPAR
LKADAPLNEPHPETFYGKGAVGYLDYPTL
>Mature_348_residues
ATIFDPIKLGDLELSNRIIMAPLTRCRADEGRVPNALMAEYYVQRASAGLILSEATSVTPMGVGYPDTPGIWSNDQVRGW
TNVTKAVHAAGGKIVLQLWHVGRISHPSYLNGETPVAPSAIQPKGHVSLVRPLADYPTPRALETAEIADIVDAYRVGAEN
AKAAGFDGVEIHGANGYLLDQFLQSSTNQRTDNYGGSLENRARLLLEVTDAAIEVWGAGRVGVHLAPRADSHDMGDDNLA
ETFTYVARELGKRGIAFICSREKEGADSLGPQLKEAFGGAYIANERFTKDSANAWLAEGKADAVAFGIPFIANPDLPARL
KADAPLNEPHPETFYGKGAVGYLDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=363, Percent_Identity=47.6584022038567, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789463, Length=340, Percent_Identity=29.7058823529412, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=258, Percent_Identity=31.0077519379845, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=28.0130293159609, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=353, Percent_Identity=27.4787535410765, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI72001454, Length=344, Percent_Identity=26.1627906976744, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=306, Percent_Identity=27.7777777777778, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559802, Length=265, Percent_Identity=29.0566037735849, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=191, Percent_Identity=30.8900523560209, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=382, Percent_Identity=35.8638743455497, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321973, Length=380, Percent_Identity=36.3157894736842, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37500; Mature: 37368

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIFDPIKLGDLELSNRIIMAPLTRCRADEGRVPNALMAEYYVQRASAGLILSEATSVT
CCCCCCCCEECCEECCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
PMGVGYPDTPGIWSNDQVRGWTNVTKAVHAAGGKIVLQLWHVGRISHPSYLNGETPVAPS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
AIQPKGHVSLVRPLADYPTPRALETAEIADIVDAYRVGAENAKAAGFDGVEIHGANGYLL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHH
DQFLQSSTNQRTDNYGGSLENRARLLLEVTDAAIEVWGAGRVGVHLAPRADSHDMGDDNL
HHHHHHCCCCCCCCCCCCHHHHHEEEEEEHHHHHEEECCCCCCEEECCCCCCCCCCCCHH
AETFTYVARELGKRGIAFICSREKEGADSLGPQLKEAFGGAYIANERFTKDSANAWLAEG
HHHHHHHHHHHCCCCEEEEECCCCCCCHHCCHHHHHHHCCEEEECCCCCCCCCCCEEECC
KADAVAFGIPFIANPDLPARLKADAPLNEPHPETFYGKGAVGYLDYPTL
CCCEEEECCCEEECCCCCCEEECCCCCCCCCCCCEECCCCEECCCCCCC
>Mature Secondary Structure 
ATIFDPIKLGDLELSNRIIMAPLTRCRADEGRVPNALMAEYYVQRASAGLILSEATSVT
CCCCCCCEECCEECCCEEEEECHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
PMGVGYPDTPGIWSNDQVRGWTNVTKAVHAAGGKIVLQLWHVGRISHPSYLNGETPVAPS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
AIQPKGHVSLVRPLADYPTPRALETAEIADIVDAYRVGAENAKAAGFDGVEIHGANGYLL
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHH
DQFLQSSTNQRTDNYGGSLENRARLLLEVTDAAIEVWGAGRVGVHLAPRADSHDMGDDNL
HHHHHHCCCCCCCCCCCCHHHHHEEEEEEHHHHHEEECCCCCCEEECCCCCCCCCCCCHH
AETFTYVARELGKRGIAFICSREKEGADSLGPQLKEAFGGAYIANERFTKDSANAWLAEG
HHHHHHHHHHHCCCCEEEEECCCCCCCHHCCHHHHHHHCCEEEECCCCCCCCCCCEEECC
KADAVAFGIPFIANPDLPARLKADAPLNEPHPETFYGKGAVGYLDYPTL
CCCEEEECCCEEECCCCCCEEECCCCCCCCCCCCEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]