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The map label for this gene is mepA [H]

Identifier: 77457507

GI number: 77457507

Start: 1456170

End: 1457327

Strand: Reverse

Name: mepA [H]

Synonym: Pfl01_1280

Alternate gene names: 77457507

Gene position: 1457327-1456170 (Counterclockwise)

Preceding gene: 77457509

Following gene: 77457506

Centisome position: 22.63

GC content: 62.61

Gene sequence:

>1158_bases
ATGCAATTCAAGCCAGCTGTTACCGCTCTGGTCACTGCCGTCGCCCTGGCATCGCTGCTCAGCGGATGTAAAAAGGAAGA
GGCGGCTCCCGCCGCACCACCTCCTCAGGTCGGCGTCGTCACCCTGCAACCACAAGCCTTTACCCTGACTTCCGAATTGC
CGGGCCGCACCAGCGCGTTCCGCATCGCCGAAGTTCGTCCGCAGGTCAACGGCATCATTCTCAAGCGTCTGTTCAAGGAA
GGCGGCGATGTCAAAGCCGGCCAGCAGCTGTATCAGATCGATCCGTCGGTCTATGAAGCGACCCTGAAAAGCGCCGAAGC
CAACCTGCGTTCGACCAAGTCGATCTCCGACCGCTACAAGCAACTGGTCGACGAGCAGGCCGTCAGCCGTCAGGAATACG
ACACCGCCGTGGCCAACCGCCTGCAATCGGAAGCGTCGTTGCAGACCGCACAGATCAACGTGCGCTACACCAAGGTCTAC
GCACCGATCTCCGGTCGTATCGGACGCTCTTCGGTGACCGAAGGCGCGCTGGTCAGCAACGGACAGGCCGATGCGATGGC
CGTGATCCAGCAACTGGACCCGATCTACGTCGACGTCACCCAGTCCTCGGTCGAATTGCTGGAACTGCGCCGCGAACTGG
AAAGTGGCCGCCTGCAAAAGGCTGGCGACAACGCCGCTGCGGTCAAGCTGACCCTCGAAGACGGCAGCCAGTACAAGCTC
GACGGTAAACTGGAATTCTCCGAAGTGTCGGTCGATCAGACCACCGGTTCCGTGACCCTGCGCGCCGTGTTCCCGAACCC
GGATCACACTCTGCTGCCGGGCATGTTCGTCCACGCGCAGTTGCAGGCCGGTGTGAACAGCGCCGCGATCCTCGCGCCGC
AGCAAGGCGTGACCCGCGACCTCAAAGGCACCCCGACCGCACTGGTCGTCGGCCCGGACAACAAGGTCGAACTGCGTCAG
CTCAAGGCCAGCCGTACTGTCGGCAGCCAGTGGCTGATCGAGGACGGCCTGAAGGCCGGCGATCGCCTGATCACCGAAGG
GCTGCAATTCGTCAAACCGGGTGTTGAGGTCAAACCGACCGAAGCGACCAACGTCGGCAACAAGAACCCGGCCCCCGCTC
AGGCAGCTGACAAAGCCGCCGGCGGCAAAGGGGAGTAA

Upstream 100 bases:

>100_bases
GTAAGTATATTCCTTAGCAAGCTACTTATCCACCCGGTAACCCTTTTTTACCCTTCCACACTTCTTGTGCGCTTTTCGCG
CGCCTGACCCGAGGATCTTC

Downstream 100 bases:

>100_bases
TCCATGTCAAAATTTTTCATCGACCGTCCGATTTTCGCCTGGGTAATTGCCCTGGTGATCATGCTGGTCGGGGCACTTTC
GATCCTGAAACTGCCGATCA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MQFKPAVTALVTAVALASLLSGCKKEEAAPAAPPPQVGVVTLQPQAFTLTSELPGRTSAFRIAEVRPQVNGIILKRLFKE
GGDVKAGQQLYQIDPSVYEATLKSAEANLRSTKSISDRYKQLVDEQAVSRQEYDTAVANRLQSEASLQTAQINVRYTKVY
APISGRIGRSSVTEGALVSNGQADAMAVIQQLDPIYVDVTQSSVELLELRRELESGRLQKAGDNAAAVKLTLEDGSQYKL
DGKLEFSEVSVDQTTGSVTLRAVFPNPDHTLLPGMFVHAQLQAGVNSAAILAPQQGVTRDLKGTPTALVVGPDNKVELRQ
LKASRTVGSQWLIEDGLKAGDRLITEGLQFVKPGVEVKPTEATNVGNKNPAPAQAADKAAGGKGE

Sequences:

>Translated_385_residues
MQFKPAVTALVTAVALASLLSGCKKEEAAPAAPPPQVGVVTLQPQAFTLTSELPGRTSAFRIAEVRPQVNGIILKRLFKE
GGDVKAGQQLYQIDPSVYEATLKSAEANLRSTKSISDRYKQLVDEQAVSRQEYDTAVANRLQSEASLQTAQINVRYTKVY
APISGRIGRSSVTEGALVSNGQADAMAVIQQLDPIYVDVTQSSVELLELRRELESGRLQKAGDNAAAVKLTLEDGSQYKL
DGKLEFSEVSVDQTTGSVTLRAVFPNPDHTLLPGMFVHAQLQAGVNSAAILAPQQGVTRDLKGTPTALVVGPDNKVELRQ
LKASRTVGSQWLIEDGLKAGDRLITEGLQFVKPGVEVKPTEATNVGNKNPAPAQAADKAAGGKGE
>Mature_385_residues
MQFKPAVTALVTAVALASLLSGCKKEEAAPAAPPPQVGVVTLQPQAFTLTSELPGRTSAFRIAEVRPQVNGIILKRLFKE
GGDVKAGQQLYQIDPSVYEATLKSAEANLRSTKSISDRYKQLVDEQAVSRQEYDTAVANRLQSEASLQTAQINVRYTKVY
APISGRIGRSSVTEGALVSNGQADAMAVIQQLDPIYVDVTQSSVELLELRRELESGRLQKAGDNAAAVKLTLEDGSQYKL
DGKLEFSEVSVDQTTGSVTLRAVFPNPDHTLLPGMFVHAQLQAGVNSAAILAPQQGVTRDLKGTPTALVVGPDNKVELRQ
LKASRTVGSQWLIEDGLKAGDRLITEGLQFVKPGVEVKPTEATNVGNKNPAPAQAADKAAGGKGE

Specific function: The periplasmic linker protein component of an organic solvent and antibiotic efflux pump; confers resistance to toluene, hexane, p-xylene, ampicillin, penicillin G, erythromycin, novobiocin and tetracycline [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=384, Percent_Identity=54.1666666666667, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=51.0989010989011, Blast_Score=348, Evalue=4e-97,
Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=44.2307692307692, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI87082036, Length=324, Percent_Identity=28.7037037037037, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40994; Mature: 40994

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFKPAVTALVTAVALASLLSGCKKEEAAPAAPPPQVGVVTLQPQAFTLTSELPGRTSAF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCEE
RIAEVRPQVNGIILKRLFKEGGDVKAGQQLYQIDPSVYEATLKSAEANLRSTKSISDRYK
EEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVDEQAVSRQEYDTAVANRLQSEASLQTAQINVRYTKVYAPISGRIGRSSVTEGALVSN
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEECC
GQADAMAVIQQLDPIYVDVTQSSVELLELRRELESGRLQKAGDNAAAVKLTLEDGSQYKL
CCHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEE
DGKLEFSEVSVDQTTGSVTLRAVFPNPDHTLLPGMFVHAQLQAGVNSAAILAPQQGVTRD
CCEEEEHEEEEECCCCCEEEEEECCCCCCCCCCCCEEEHHHHCCCCCEEEECCCCCCCCC
LKGTPTALVVGPDNKVELRQLKASRTVGSQWLIEDGLKAGDRLITEGLQFVKPGVEVKPT
CCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
EATNVGNKNPAPAQAADKAAGGKGE
CCCCCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure
MQFKPAVTALVTAVALASLLSGCKKEEAAPAAPPPQVGVVTLQPQAFTLTSELPGRTSAF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCEE
RIAEVRPQVNGIILKRLFKEGGDVKAGQQLYQIDPSVYEATLKSAEANLRSTKSISDRYK
EEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVDEQAVSRQEYDTAVANRLQSEASLQTAQINVRYTKVYAPISGRIGRSSVTEGALVSN
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEEEECCCCCCCCCCCCCCCEECC
GQADAMAVIQQLDPIYVDVTQSSVELLELRRELESGRLQKAGDNAAAVKLTLEDGSQYKL
CCHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEE
DGKLEFSEVSVDQTTGSVTLRAVFPNPDHTLLPGMFVHAQLQAGVNSAAILAPQQGVTRD
CCEEEEHEEEEECCCCCEEEEEECCCCCCCCCCCCEEEHHHHCCCCCEEEECCCCCCCCC
LKGTPTALVVGPDNKVELRQLKASRTVGSQWLIEDGLKAGDRLITEGLQFVKPGVEVKPT
CCCCCCEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCC
EATNVGNKNPAPAQAADKAAGGKGE
CCCCCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9827328 [H]