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The map label for this gene is gcvA [H]

Identifier: 77457486

GI number: 77457486

Start: 1433350

End: 1434216

Strand: Reverse

Name: gcvA [H]

Synonym: Pfl01_1259

Alternate gene names: 77457486

Gene position: 1434216-1433350 (Counterclockwise)

Preceding gene: 77457489

Following gene: 77457485

Centisome position: 22.28

GC content: 64.36

Gene sequence:

>867_bases
GTGCCCCTGCATCAGGATCTGCCACCGCTGATGGCCTTGCGCGCCTTTGAGGCCGTGGCCCGCCATTTGAGCTTCATCAA
GGCTGCCGCTGAGCTGTCGGTGACCCAAAGCGCGATCAGCCATCAGGTGCACAAACTCGAAGAGTTCCTCGATCAGCGAC
TGTTCGTGCGCCGCACCCGGGCGATTGATCTGACACCCGCCGGCGAACATTACTACCGGCAGATCGAACCGGCCCTCGCT
GCGATTGCCGCCGCCACCCTCCACCTGCGCGGCGAACGCCCCACCACCCTGCGCATCGGTCTGCTCGCCTCTTTCGCGAC
CCTGTGGCTGGCACCGCGTCTGGCGGATTTCAACGCGAAATACCCGAACATCCATGTCGAACTGCTGCCCGCCGTGCAAC
TGGCCGACGTCGACGCGGGCGAGGTGGATCTGGCGATCCGCTACGGCAAGGGCGGCTGGCCGAAAGTCCAGGCCCGCCGG
TTCATGGGCGAAACCCTGATGCCGGTATGCAGCCCGGCCTTCAAGGCCAGAGGCGTGAGCAACGGACCGTTGCTGATGGC
CAAGTCGCACCAGCCGTTCGAATGGATCGACTGGCAGCAACACAGCGGCATCGATCTGACTCATGTACCCAGCGTCATGC
TGCATGACTACAACATCGTCGTCGAAGCGGCCGTCGCCGGTCAGGGCATTGCCATGGGCCGTGAACGAATGATCGATCGG
CGGATCAAGGAGGGCGCGCTGGTGCCAGCCTTCGACACGCCGCCAATGCTCGGCGAGATCGGTTATTGGCTGGTCACCCC
GCAACGCCCGGCCACTGCGGCGGCGCAGAGTTTCTGCCAGTGGCTGGAGGAAACCGCGAACGCATGA

Upstream 100 bases:

>100_bases
GACCAGCCACTGACCTCAACGCTCTACAACAATTCATGTACCGCCCACCGGATTTGCCCGCACAATCGGCACCTCGCTCT
GAACCCGAAGGATCTGCGCC

Downstream 100 bases:

>100_bases
GTTTTTTGGATGCGTCGAATTAAATCTATCCGTTTGTCGCCCTTTCCTCGCCACAACATGCTGAAGCCCTCACTCAGGAG
GACTTCCCATGACCGATTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MPLHQDLPPLMALRAFEAVARHLSFIKAAAELSVTQSAISHQVHKLEEFLDQRLFVRRTRAIDLTPAGEHYYRQIEPALA
AIAAATLHLRGERPTTLRIGLLASFATLWLAPRLADFNAKYPNIHVELLPAVQLADVDAGEVDLAIRYGKGGWPKVQARR
FMGETLMPVCSPAFKARGVSNGPLLMAKSHQPFEWIDWQQHSGIDLTHVPSVMLHDYNIVVEAAVAGQGIAMGRERMIDR
RIKEGALVPAFDTPPMLGEIGYWLVTPQRPATAAAQSFCQWLEETANA

Sequences:

>Translated_288_residues
MPLHQDLPPLMALRAFEAVARHLSFIKAAAELSVTQSAISHQVHKLEEFLDQRLFVRRTRAIDLTPAGEHYYRQIEPALA
AIAAATLHLRGERPTTLRIGLLASFATLWLAPRLADFNAKYPNIHVELLPAVQLADVDAGEVDLAIRYGKGGWPKVQARR
FMGETLMPVCSPAFKARGVSNGPLLMAKSHQPFEWIDWQQHSGIDLTHVPSVMLHDYNIVVEAAVAGQGIAMGRERMIDR
RIKEGALVPAFDTPPMLGEIGYWLVTPQRPATAAAQSFCQWLEETANA
>Mature_287_residues
PLHQDLPPLMALRAFEAVARHLSFIKAAAELSVTQSAISHQVHKLEEFLDQRLFVRRTRAIDLTPAGEHYYRQIEPALAA
IAAATLHLRGERPTTLRIGLLASFATLWLAPRLADFNAKYPNIHVELLPAVQLADVDAGEVDLAIRYGKGGWPKVQARRF
MGETLMPVCSPAFKARGVSNGPLLMAKSHQPFEWIDWQQHSGIDLTHVPSVMLHDYNIVVEAAVAGQGIAMGRERMIDRR
IKEGALVPAFDTPPMLGEIGYWLVTPQRPATAAAQSFCQWLEETANA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=34.7972972972973, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=29.757785467128, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786448, Length=285, Percent_Identity=31.5789473684211, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787589, Length=283, Percent_Identity=30.0353356890459, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=26.3513513513513, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787128, Length=252, Percent_Identity=28.968253968254, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI157672245, Length=162, Percent_Identity=34.5679012345679, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789639, Length=165, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787601, Length=174, Percent_Identity=27.5862068965517, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=137, Percent_Identity=32.1167883211679, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87081978, Length=283, Percent_Identity=27.9151943462898, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693193, Length=169, Percent_Identity=28.9940828402367, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=242, Percent_Identity=25.6198347107438, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787879, Length=144, Percent_Identity=32.6388888888889, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31920; Mature: 31789

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHQDLPPLMALRAFEAVARHLSFIKAAAELSVTQSAISHQVHKLEEFLDQRLFVRRTR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AIDLTPAGEHYYRQIEPALAAIAAATLHLRGERPTTLRIGLLASFATLWLAPRLADFNAK
EEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
YPNIHVELLPAVQLADVDAGEVDLAIRYGKGGWPKVQARRFMGETLMPVCSPAFKARGVS
CCCEEEEEECCEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
NGPLLMAKSHQPFEWIDWQQHSGIDLTHVPSVMLHDYNIVVEAAVAGQGIAMGRERMIDR
CCCEEEECCCCCCCCCCCCCCCCCCHHHCCHHHEECCEEEEEEECCCCCHHHHHHHHHHH
RIKEGALVPAFDTPPMLGEIGYWLVTPQRPATAAAQSFCQWLEETANA
HHHCCCEECCCCCCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLHQDLPPLMALRAFEAVARHLSFIKAAAELSVTQSAISHQVHKLEEFLDQRLFVRRTR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AIDLTPAGEHYYRQIEPALAAIAAATLHLRGERPTTLRIGLLASFATLWLAPRLADFNAK
EEECCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
YPNIHVELLPAVQLADVDAGEVDLAIRYGKGGWPKVQARRFMGETLMPVCSPAFKARGVS
CCCEEEEEECCEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
NGPLLMAKSHQPFEWIDWQQHSGIDLTHVPSVMLHDYNIVVEAAVAGQGIAMGRERMIDR
CCCEEEECCCCCCCCCCCCCCCCCCHHHCCHHHEECCEEEEEEECCCCCHHHHHHHHHHH
RIKEGALVPAFDTPPMLGEIGYWLVTPQRPATAAAQSFCQWLEETANA
HHHCCCEECCCCCCCCHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]