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The map label for this gene is pitA [H]

Identifier: 77457489

GI number: 77457489

Start: 1435392

End: 1436867

Strand: Reverse

Name: pitA [H]

Synonym: Pfl01_1262

Alternate gene names: 77457489

Gene position: 1436867-1435392 (Counterclockwise)

Preceding gene: 77457491

Following gene: 77457486

Centisome position: 22.32

GC content: 61.45

Gene sequence:

>1476_bases
ATGATCGATTTATTCAGCGGACTGGATGCCTGGGTGCTTGTGAGCCTCCTGCTCGCCCTGACATTTGTCCTCGCCTTCGA
GTTCATCAATGGATTTCATGACACCGCTAACGCGGTAGCCACTGTTATCTACACCAAAGCGATGCCGCCTCACCTGGCGG
TGTTCTTCTCGGGCGTGTTCAACTTCCTCGGCGTACTGCTGGGCGGCGTTGGCGTGGCGTATGCCATCGTGCACCTGCTG
CCGGTAGAACTGTTGATCAACGTAAACACCGGCCATGGTCTGGCGATGGTGTTCTCGCTGCTCGCCGCCGCGATTGCCTG
GAACCTGGGCACCTGGTACTTCGGTATCCCGGCCTCCAGTTCGCACACGTTGATCGGTTCGATCCTCGGTGTCGGCCTGG
CCAACGCCCTGATCAACGAAATTCCACTGGCTGACGGCGTCAACTGGCAGAAAGCGATCGACATCGGCGCCTCGCTGGTG
TTCTCGCCGATGGCCGGCTTCCTGATCGCCGCGCTGGTGCTGATCGGCCTGAAATGGTGGCGCCCGCTGTCGAAGATGCA
CAAGACGCCGGAACAGCGCCGCAAGATCGACGACAAGAAGCACCCACCGTTCTGGAACCGCCTGGTCCTGGTGATCTCGG
CAATGGCCGTGAGCTTCGTGCACGGCTCCAACGACGGCCAGAAAGGTATCGGCCTGATCATGCTGGTACTGATCGGTATC
GTGCCTGCGCAGTTCGTACTCGACCTGAACAGCACTACCTACCAGATCGAACGCACTCGCGATGCGACCCTGCACCTGAG
CCAGTTCTACAAGCGCAACGCCGATACCCTGGGCGAATTCCTGGCCCTGGGCAAAAGCGTGGAAGGCGACCTGCCGGAGA
AATTCCGTTGCAACCCGCAACAGACCGAACCGACCATCGCCGCCCTGCTCGACACGCTCAAAGGTGTAGCTGACTACCAC
TCGCTGTCCGCTGAAAGCCGCATCGAAGTGCGTCGCTACCTGCTCTGCCTGGACGACACCGCGAAGAAAGTCAGCAAACT
GCCAGGCCTCGCCGCTCGTGAAAAAGCCGACCTGGACAAACTGCGCAAAGACCTGACCACCACCACCGAATACGCCCCGT
TCTGGGTGATTCTGGCGGTAGCGCTGGCCCTGGGCCTGGGCACCATGGTCGGCTGGAAACGCGTGGTACTGACCATCGGC
GAGAAGATCGGCAAGCAAGGCATGACCTACTCGCAAGGCATGTCGGCCCAGATCACCACCGCCAGCCTGATCGGCATGGC
CAACATCTTCAGCCTGCCGGTGTCCACCACCCACGTCCTCTCCTCGGGCGTGGCCGGCACCATGGTCGCCAACAAAAGCG
GCCTGCAAGGCGGCACGGTGAAAACCATCCTGCTGGCCTGGGTCCTGACCCTGCCAGCCACCGTGGCGCTGTCGGCCGGC
CTGTTCTGGCTGGCGTCGAAGGCGCTGGGTAGCTGA

Upstream 100 bases:

>100_bases
TTAATTTGGCGAAGAAGCTTACCGATGAGCAGGTAGGGGATCGATTTCCAGTCTTTTCTGGCATAATCGCGCCCCCTTAT
GACCGGGTCAGAAAACCTTC

Downstream 100 bases:

>100_bases
TAGACCGCTGAAATGAAAAAGGCGTGATTCGAAAGGATCACGCCTTTTTTGTTGCTTCCAGAGATGCGACGCGGAGCGTC
CCGGGCTGCATTCCCACGCG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIDLFSGLDAWVLVSLLLALTFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLL
PVELLINVNTGHGLAMVFSLLAAAIAWNLGTWYFGIPASSSHTLIGSILGVGLANALINEIPLADGVNWQKAIDIGASLV
FSPMAGFLIAALVLIGLKWWRPLSKMHKTPEQRRKIDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPAQFVLDLNSTTYQIERTRDATLHLSQFYKRNADTLGEFLALGKSVEGDLPEKFRCNPQQTEPTIAALLDTLKGVADYH
SLSAESRIEVRRYLLCLDDTAKKVSKLPGLAAREKADLDKLRKDLTTTTEYAPFWVILAVALALGLGTMVGWKRVVLTIG
EKIGKQGMTYSQGMSAQITTASLIGMANIFSLPVSTTHVLSSGVAGTMVANKSGLQGGTVKTILLAWVLTLPATVALSAG
LFWLASKALGS

Sequences:

>Translated_491_residues
MIDLFSGLDAWVLVSLLLALTFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLL
PVELLINVNTGHGLAMVFSLLAAAIAWNLGTWYFGIPASSSHTLIGSILGVGLANALINEIPLADGVNWQKAIDIGASLV
FSPMAGFLIAALVLIGLKWWRPLSKMHKTPEQRRKIDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPAQFVLDLNSTTYQIERTRDATLHLSQFYKRNADTLGEFLALGKSVEGDLPEKFRCNPQQTEPTIAALLDTLKGVADYH
SLSAESRIEVRRYLLCLDDTAKKVSKLPGLAAREKADLDKLRKDLTTTTEYAPFWVILAVALALGLGTMVGWKRVVLTIG
EKIGKQGMTYSQGMSAQITTASLIGMANIFSLPVSTTHVLSSGVAGTMVANKSGLQGGTVKTILLAWVLTLPATVALSAG
LFWLASKALGS
>Mature_491_residues
MIDLFSGLDAWVLVSLLLALTFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVFNFLGVLLGGVGVAYAIVHLL
PVELLINVNTGHGLAMVFSLLAAAIAWNLGTWYFGIPASSSHTLIGSILGVGLANALINEIPLADGVNWQKAIDIGASLV
FSPMAGFLIAALVLIGLKWWRPLSKMHKTPEQRRKIDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
VPAQFVLDLNSTTYQIERTRDATLHLSQFYKRNADTLGEFLALGKSVEGDLPEKFRCNPQQTEPTIAALLDTLKGVADYH
SLSAESRIEVRRYLLCLDDTAKKVSKLPGLAAREKADLDKLRKDLTTTTEYAPFWVILAVALALGLGTMVGWKRVVLTIG
EKIGKQGMTYSQGMSAQITTASLIGMANIFSLPVSTTHVLSSGVAGTMVANKSGLQGGTVKTILLAWVLTLPATVALSAG
LFWLASKALGS

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=499, Percent_Identity=54.7094188376754, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI1789360, Length=495, Percent_Identity=52.9292929292929, Blast_Score=511, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 52736; Mature: 52736

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLFSGLDAWVLVSLLLALTFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NFLGVLLGGVGVAYAIVHLLPVELLINVNTGHGLAMVFSLLAAAIAWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSILGVGLANALINEIPLADGVNWQKAIDIGASLVFSPMAGFLIAALVLIGLKWW
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPLSKMHKTPEQRRKIDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
HHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VPAQFVLDLNSTTYQIERTRDATLHLSQFYKRNADTLGEFLALGKSVEGDLPEKFRCNPQ
HHHHHHEECCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCC
QTEPTIAALLDTLKGVADYHSLSAESRIEVRRYLLCLDDTAKKVSKLPGLAAREKADLDK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRKDLTTTTEYAPFWVILAVALALGLGTMVGWKRVVLTIGEKIGKQGMTYSQGMSAQITT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEHH
ASLIGMANIFSLPVSTTHVLSSGVAGTMVANKSGLQGGTVKTILLAWVLTLPATVALSAG
HHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFWLASKALGS
HHHHHHHHHCC
>Mature Secondary Structure
MIDLFSGLDAWVLVSLLLALTFVLAFEFINGFHDTANAVATVIYTKAMPPHLAVFFSGVF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
NFLGVLLGGVGVAYAIVHLLPVELLINVNTGHGLAMVFSLLAAAIAWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSILGVGLANALINEIPLADGVNWQKAIDIGASLVFSPMAGFLIAALVLIGLKWW
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPLSKMHKTPEQRRKIDDKKHPPFWNRLVLVISAMAVSFVHGSNDGQKGIGLIMLVLIGI
HHHHHHHCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VPAQFVLDLNSTTYQIERTRDATLHLSQFYKRNADTLGEFLALGKSVEGDLPEKFRCNPQ
HHHHHHEECCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHCCCCC
QTEPTIAALLDTLKGVADYHSLSAESRIEVRRYLLCLDDTAKKVSKLPGLAAREKADLDK
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
LRKDLTTTTEYAPFWVILAVALALGLGTMVGWKRVVLTIGEKIGKQGMTYSQGMSAQITT
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCEEHH
ASLIGMANIFSLPVSTTHVLSSGVAGTMVANKSGLQGGTVKTILLAWVLTLPATVALSAG
HHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFWLASKALGS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]