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The map label for this gene is rimO [H]

Identifier: 77457418

GI number: 77457418

Start: 1357707

End: 1359044

Strand: Reverse

Name: rimO [H]

Synonym: Pfl01_1191

Alternate gene names: 77457418

Gene position: 1359044-1357707 (Counterclockwise)

Preceding gene: 77457420

Following gene: 77457417

Centisome position: 21.11

GC content: 61.73

Gene sequence:

>1338_bases
ATGTCCACCACTCCTGCGCCGGCCAATCCAAAGGTTGGCTTCGTTTCTCTGGGTTGCCCGAAAGCACTGGTCGACTCCGA
GCGCATCCTGACCCAGCTGCGCATGGAAGGCTATGACGTGGTGTCCACCTATCAGGACGCCGACGTGGTCGTGGTCAACA
CCTGCGGCTTCATCGACTCGGCCAAGGCCGAATCGCTGGAAGTCATCGGCGAAGCGATCAAGGAAAACGGCAAGGTCATC
GTGACCGGCTGCATGGGCGTCGAAGAAGGCAATATCCGTGACGTGCACCCGAGCGTGCTGGCCGTCACCGGTCCGCAGCA
GTACGAGCAAGTGGTCAACGCGGTGCACGAAGTCGTGCCGCCGAAGCAGGATCACAACCCGCTGATCGACCTGGTGCCGC
CGCAAGGCATCAAGCTGACCCCGCGCCACTACGCGTACCTGAAGATTTCCGAAGGCTGCAACCACAGCTGCAGCTTCTGC
ATCATCCCGTCGATGCGCGGCAAACTGGTCAGCCGTCCGGTCGGCGACGTGCTCGACGAAGCCCAGCGTCTGGTCAAATC
CGGCGTGAAGGAACTGCTGGTGATTTCCCAGGACACCAGCGCCTATGGCGTTGACGTCAAATACCGCACCGGTTTCTGGA
ACGGCGCGCCGGTGAAAACCCGCATGACCGAACTGTGCGAAGCGCTGAGCACCCTCGGCGTCTGGGTCCGTCTGCACTAC
GTTTACCCGTACCCGCACGTCGACGAGCTGATCCCGCTGATGGCCGCCGGCAAGATCCTGCCGTACCTGGACATCCCGTT
CCAGCACGCCAGCCCGAAAGTCCTCAAGTCGATGAAACGCCCGGCGTTCGAAGACAAGACCCTGGCGCGGATCAAGAACT
GGCGCGAGATCTGCCCGGACCTGATCATCCGTTCGACCTTCATCGTCGGCTTCCCCGGCGAAACCGAAGAAGACTTCCAG
TACCTGCTGGACTGGCTGACCGAAGCCCAGCTCGACCGCGTCGGCTGCTTCCAGTATTCGCCGGTCGAAGGCGCTCCAGC
CAACGATCTGGATCTGGAAGTGGTTCCGGACGACGTCAAGCAAGACCGCTGGGAACGCTTCATGGCGCACCAGCAGGCCA
TCAGTTCGGCGCGCCTGCAACTGCGCATCGGCCGTGAAATCGAAGTACTGGTGGACGAAGTCGACGAGCAGGGCGCGGTC
GGCCGCTGCTTCTTCGATGCCCCGGAAATCGACGGCAATGTGTTCATCGACAACGGCAGCAACCTGAAGCCAGGCGACAA
GGTCTGGTGCAAGGTGACCGACGCCGACGAATACGACCTCTGGGCCGAACAGATCTAA

Upstream 100 bases:

>100_bases
TCGCTAGAATTGCGCACTTTTTGATCAGAGGCGCAGTTCAAGCGCCCATCCGTCGGTTACCGAACCAGGCAAGCGACGTC
ACAAAACACCGAGGTTAGAC

Downstream 100 bases:

>100_bases
GCGCGAAAAATACAGAAAGCCCCGCTCTCTTTTACGAGATGCGGGGCTTTTTTACGGCTATCGTTTGGGGTGGGAAGGAC
TCCCTCATCCATGACAAAGA

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSTTPAPANPKVGFVSLGCPKALVDSERILTQLRMEGYDVVSTYQDADVVVVNTCGFIDSAKAESLEVIGEAIKENGKVI
VTGCMGVEEGNIRDVHPSVLAVTGPQQYEQVVNAVHEVVPPKQDHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFC
IIPSMRGKLVSRPVGDVLDEAQRLVKSGVKELLVISQDTSAYGVDVKYRTGFWNGAPVKTRMTELCEALSTLGVWVRLHY
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKVLKSMKRPAFEDKTLARIKNWREICPDLIIRSTFIVGFPGETEEDFQ
YLLDWLTEAQLDRVGCFQYSPVEGAPANDLDLEVVPDDVKQDRWERFMAHQQAISSARLQLRIGREIEVLVDEVDEQGAV
GRCFFDAPEIDGNVFIDNGSNLKPGDKVWCKVTDADEYDLWAEQI

Sequences:

>Translated_445_residues
MSTTPAPANPKVGFVSLGCPKALVDSERILTQLRMEGYDVVSTYQDADVVVVNTCGFIDSAKAESLEVIGEAIKENGKVI
VTGCMGVEEGNIRDVHPSVLAVTGPQQYEQVVNAVHEVVPPKQDHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFC
IIPSMRGKLVSRPVGDVLDEAQRLVKSGVKELLVISQDTSAYGVDVKYRTGFWNGAPVKTRMTELCEALSTLGVWVRLHY
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKVLKSMKRPAFEDKTLARIKNWREICPDLIIRSTFIVGFPGETEEDFQ
YLLDWLTEAQLDRVGCFQYSPVEGAPANDLDLEVVPDDVKQDRWERFMAHQQAISSARLQLRIGREIEVLVDEVDEQGAV
GRCFFDAPEIDGNVFIDNGSNLKPGDKVWCKVTDADEYDLWAEQI
>Mature_444_residues
STTPAPANPKVGFVSLGCPKALVDSERILTQLRMEGYDVVSTYQDADVVVVNTCGFIDSAKAESLEVIGEAIKENGKVIV
TGCMGVEEGNIRDVHPSVLAVTGPQQYEQVVNAVHEVVPPKQDHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCI
IPSMRGKLVSRPVGDVLDEAQRLVKSGVKELLVISQDTSAYGVDVKYRTGFWNGAPVKTRMTELCEALSTLGVWVRLHYV
YPYPHVDELIPLMAAGKILPYLDIPFQHASPKVLKSMKRPAFEDKTLARIKNWREICPDLIIRSTFIVGFPGETEEDFQY
LLDWLTEAQLDRVGCFQYSPVEGAPANDLDLEVVPDDVKQDRWERFMAHQQAISSARLQLRIGREIEVLVDEVDEQGAVG
RCFFDAPEIDGNVFIDNGSNLKPGDKVWCKVTDADEYDLWAEQI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=419, Percent_Identity=27.4463007159904, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=26.5690376569038, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=26.5690376569038, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI1787057, Length=437, Percent_Identity=71.395881006865, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=399, Percent_Identity=29.0726817042606, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=472, Percent_Identity=25.2118644067797, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71996771, Length=315, Percent_Identity=27.6190476190476, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI19922432, Length=467, Percent_Identity=25.0535331905782, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=399, Percent_Identity=24.812030075188, Blast_Score=118, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49559; Mature: 49428

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS50926 TRAM ; PS00430 TONB_DEPENDENT_REC_1 ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPAPANPKVGFVSLGCPKALVDSERILTQLRMEGYDVVSTYQDADVVVVNTCGFIDS
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCC
AKAESLEVIGEAIKENGKVIVTGCMGVEEGNIRDVHPSVLAVTGPQQYEQVVNAVHEVVP
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHCC
PKQDHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLDE
CCCCCCCEEEECCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
AQRLVKSGVKELLVISQDTSAYGVDVKYRTGFWNGAPVKTRMTELCEALSTLGVWVRLHY
HHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEEEE
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKVLKSMKRPAFEDKTLARIKNWREICPD
ECCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LIIRSTFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVEGAPANDLDLEVVPDDVK
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEECCCHHH
QDRWERFMAHQQAISSARLQLRIGREIEVLVDEVDEQGAVGRCFFDAPEIDGNVFIDNGS
HHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
NLKPGDKVWCKVTDADEYDLWAEQI
CCCCCCCEEEEEECCCCCCHHHHCC
>Mature Secondary Structure 
STTPAPANPKVGFVSLGCPKALVDSERILTQLRMEGYDVVSTYQDADVVVVNTCGFIDS
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCC
AKAESLEVIGEAIKENGKVIVTGCMGVEEGNIRDVHPSVLAVTGPQQYEQVVNAVHEVVP
CCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCEEEEECHHHHHHHHHHHHHHCC
PKQDHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLDE
CCCCCCCEEEECCCCCCEECCCEEEEEEECCCCCCCCCEEEECCCCCHHHCCCHHHHHHH
AQRLVKSGVKELLVISQDTSAYGVDVKYRTGFWNGAPVKTRMTELCEALSTLGVWVRLHY
HHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHEEEEEEEE
VYPYPHVDELIPLMAAGKILPYLDIPFQHASPKVLKSMKRPAFEDKTLARIKNWREICPD
ECCCCCHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LIIRSTFIVGFPGETEEDFQYLLDWLTEAQLDRVGCFQYSPVEGAPANDLDLEVVPDDVK
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEECCCHHH
QDRWERFMAHQQAISSARLQLRIGREIEVLVDEVDEQGAVGRCFFDAPEIDGNVFIDNGS
HHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
NLKPGDKVWCKVTDADEYDLWAEQI
CCCCCCCEEEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA