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The map label for this gene is gcvA [H]

Identifier: 77456796

GI number: 77456796

Start: 661792

End: 662694

Strand: Reverse

Name: gcvA [H]

Synonym: Pfl01_0568

Alternate gene names: 77456796

Gene position: 662694-661792 (Counterclockwise)

Preceding gene: 77456800

Following gene: 77456795

Centisome position: 10.29

GC content: 64.89

Gene sequence:

>903_bases
ATGTTCGCTTCATTGCCGCTGACCGCCCTGCGCGCGTTCGAATCCGCCTCCCGTCTGCTGAGTTTCAAGGCAGCCGCCGA
AGAGCTGGCGGTGACGCCGACGGCGATTTCCCATCAGATCCGCTCGCTGGAGGATTGGCTCGGCGTGGCGCTGTTCGAAC
GCCTGCCGCGTCAGGTGCGCCTGACCGAGGGTGGCGAGCGGCTGTTCCGCAGCCTGCACGGCGCATTTCTGGAAGTGGCG
CAAAGCGTCGACACCCTGCGCCCGCAACGCAGCGGCAGCAGCCTCACGCTGTCGACCACCGCCGCGTTCGCCGCGCTGTG
GCTGGTGCCGCGACTGGGGCGGTTTTATGCGAAGCATCCGAACATCAATGTGCGGCTGGACACGCATTGCGAAGTCATCG
ACCTGCATCAGGACGCCAGCGTCGACCTGGTATTGCGCTACAGCCTCGACGATTACCCGAACCTCTATGGGTTGTGCCTG
TTCGATGAATCGTTCGGCGTATACGGCTCGCCGGAACAGGTGGCGCTGGCCGCCCGCCGCACTCCGGCGCTGATCAGCGT
GCATTGGCACAATTCGAAGCTCTACGCCCACGGCTGGGAGGCCTGGTGCGCGCAGTCGGGCGAGAACTGGCTGAATCAGC
ACCCGGCCGTCCGCGAATACGATGAAGAGCATTACGCCCTGCAAGCGGCCATTGCCGGACAAGGGCTGGTGCTGGCGAGC
AACATCCTGGTGTCGCAAAGCGTCGCCAGCGGTCTGCTTGTGCCGTACAAGGGCGAGGTCCAGGTCGATGGCGCCGGTTA
CAGCGCACTTTGCGTACCGGGTCGCGAGCGGCATCCACCGGTGAAGGCGTTTTTTGCCTGGCTGCGCGAAGAGGCACAGC
TTTCCGGGCATGTGCCGCGCTGA

Upstream 100 bases:

>100_bases
GACTTGAGTGACTGTGTGGCGCCAGATTGCAGGCATCACCTCATCGCCTCAAATGAGCAAAAATCAGTCGGGATGAATTG
ATTTCATCCGTGGAGTTTCC

Downstream 100 bases:

>100_bases
ATCCGACAAAACTTTTTGCAGCATTCATCACTCACAACCTGGTAACGATCACGTCAGCAGAACGTGACGCCAATCGGATT
AGCCTCCAGGAGACTGAAAT

Product: regulatory protein, LysR

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MFASLPLTALRAFESASRLLSFKAAAEELAVTPTAISHQIRSLEDWLGVALFERLPRQVRLTEGGERLFRSLHGAFLEVA
QSVDTLRPQRSGSSLTLSTTAAFAALWLVPRLGRFYAKHPNINVRLDTHCEVIDLHQDASVDLVLRYSLDDYPNLYGLCL
FDESFGVYGSPEQVALAARRTPALISVHWHNSKLYAHGWEAWCAQSGENWLNQHPAVREYDEEHYALQAAIAGQGLVLAS
NILVSQSVASGLLVPYKGEVQVDGAGYSALCVPGRERHPPVKAFFAWLREEAQLSGHVPR

Sequences:

>Translated_300_residues
MFASLPLTALRAFESASRLLSFKAAAEELAVTPTAISHQIRSLEDWLGVALFERLPRQVRLTEGGERLFRSLHGAFLEVA
QSVDTLRPQRSGSSLTLSTTAAFAALWLVPRLGRFYAKHPNINVRLDTHCEVIDLHQDASVDLVLRYSLDDYPNLYGLCL
FDESFGVYGSPEQVALAARRTPALISVHWHNSKLYAHGWEAWCAQSGENWLNQHPAVREYDEEHYALQAAIAGQGLVLAS
NILVSQSVASGLLVPYKGEVQVDGAGYSALCVPGRERHPPVKAFFAWLREEAQLSGHVPR
>Mature_300_residues
MFASLPLTALRAFESASRLLSFKAAAEELAVTPTAISHQIRSLEDWLGVALFERLPRQVRLTEGGERLFRSLHGAFLEVA
QSVDTLRPQRSGSSLTLSTTAAFAALWLVPRLGRFYAKHPNINVRLDTHCEVIDLHQDASVDLVLRYSLDDYPNLYGLCL
FDESFGVYGSPEQVALAARRTPALISVHWHNSKLYAHGWEAWCAQSGENWLNQHPAVREYDEEHYALQAAIAGQGLVLAS
NILVSQSVASGLLVPYKGEVQVDGAGYSALCVPGRERHPPVKAFFAWLREEAQLSGHVPR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=33.5593220338983, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=32.6732673267327, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=32.3232323232323, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787128, Length=206, Percent_Identity=31.5533980582524, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081978, Length=267, Percent_Identity=29.9625468164794, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=281, Percent_Identity=23.8434163701068, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=28.169014084507, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=25.2525252525253, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33213; Mature: 33213

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFASLPLTALRAFESASRLLSFKAAAEELAVTPTAISHQIRSLEDWLGVALFERLPRQVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTEGGERLFRSLHGAFLEVAQSVDTLRPQRSGSSLTLSTTAAFAALWLVPRLGRFYAKHP
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
NINVRLDTHCEVIDLHQDASVDLVLRYSLDDYPNLYGLCLFDESFGVYGSPEQVALAARR
CEEEEECCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHEECC
TPALISVHWHNSKLYAHGWEAWCAQSGENWLNQHPAVREYDEEHYALQAAIAGQGLVLAS
CCEEEEEEEECCEEEECCHHHHHHCCCCHHHHCCCCHHHCCCHHHEEHHHHCCCCEEEHH
NILVSQSVASGLLVPYKGEVQVDGAGYSALCVPGRERHPPVKAFFAWLREEAQLSGHVPR
HHHHHHHHHCCCEECCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFASLPLTALRAFESASRLLSFKAAAEELAVTPTAISHQIRSLEDWLGVALFERLPRQVR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTEGGERLFRSLHGAFLEVAQSVDTLRPQRSGSSLTLSTTAAFAALWLVPRLGRFYAKHP
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
NINVRLDTHCEVIDLHQDASVDLVLRYSLDDYPNLYGLCLFDESFGVYGSPEQVALAARR
CEEEEECCCCEEEEECCCCCEEEEEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHEECC
TPALISVHWHNSKLYAHGWEAWCAQSGENWLNQHPAVREYDEEHYALQAAIAGQGLVLAS
CCEEEEEEEECCEEEECCHHHHHHCCCCHHHHCCCCHHHCCCHHHEEHHHHCCCCEEEHH
NILVSQSVASGLLVPYKGEVQVDGAGYSALCVPGRERHPPVKAFFAWLREEAQLSGHVPR
HHHHHHHHHCCCEECCCCCEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]