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The map label for this gene is livJ [C]

Identifier: 77456780

GI number: 77456780

Start: 645744

End: 646877

Strand: Direct

Name: livJ [C]

Synonym: Pfl01_0552

Alternate gene names: 77456780

Gene position: 645744-646877 (Clockwise)

Preceding gene: 77456779

Following gene: 77456781

Centisome position: 10.03

GC content: 60.85

Gene sequence:

>1134_bases
ATGTCCCAGACGTTTTACAAGAAAGGCTTTCTGGCCCTCGCAGTGGCTACTGCGCTGGGTGTTTCTGCGTTTGCACAAGC
TGATGTGAAAATCGGTGTGGCGGGTCCGATGACGGGCGCCAACGCGGCATTTGGCGAGCAATACATGAAAGGGGCACAAG
CGGCGGCCGACGCGGTCAACGCGGCGGGCGGCGTCAACGGGGAGAAGATCGTCCTGGTCAAGGGCGATGACGCTTGCGAG
CCGAAACAGGCGGTGACGGTCGCCAAGGACCTCACCAACCAGAAAGTCGCCGGTGTGGTCGGTCACTTCTGCTCGTCCTC
CACCATTCCTGCGTCCGAAATCTACGACGAAGCCGGGATCATTGCCATCACCCCGGGTTCCACCAACCCGCAAGTGACCG
AGCGCGGTCTGAGCGCCATGTTCCGTATGTGCGGCCGTGACGACCAGCAAGGCATCGTCGCCGGCGATTACATCGTCGAC
GTGCTCAAGGGCAAGAAAGTCGTCGTGCTGCACGACAAGGACACCTATGGTCAGGGCCTGGCCGATGCGACCAAGGCACA
ACTGGAAAAACGCGGCGTGAAGCCGGTGCTGTACGAAGGCCTGACCCGTGGCGAGAAAGACTTCAGCACAATCGTCACCA
AGATCCGTGGCGCCGGCGCCGATGTCGTCTACTTCGGTGGCCTGCACCCGGAAGCCGGCCCGCTGGTTCGCCAACTGCGT
GAACAAGGCCTGAAAGACGTCAAGTTCATGTCCGATGACGGCATCGTGACCGATGAACTGGTGACCACCGCCGGTGGCCC
GCAATTCGTCGACGGCGTGCTGATGACGTTTGGTGCCGACCCGCGCCTGCTGCCAGACAGCAAGACCGTGGTAGATGCAT
TCCGCAAGGCTGGCACCGAGCCTGAAGGCTACACCCTGTACGCCTATGCATCGGTTCAGGCCCTGGCCGCCGCCTTCAAC
GGCGCCAAGTCGAACAAGGGCGAGGAGGCCGCTGCCTGGCTGAAGAAGAACCCGGTCAAGACCGTCATGGGCGAGAAGAC
CTGGGATTCCAAAGGCGACCTGAAGGTCTCCGACTACGTGGTCTACCAATGGGACAAGGACGGCAAATATCACCAGCTGG
AAAAACAGAAATAA

Upstream 100 bases:

>100_bases
GCGACTTTTTGAAGAATAAAAAACCGATGGCACGGCCCTTGCTCTGAGCATTCAAGTGAAGTCGCAGTGCCAACTAAAAA
AAACCTTGGAGCACCACCTC

Downstream 100 bases:

>100_bases
CGGCACCTGATCAACCGGACGCCACATTCCCTGTGGGAGCGGGCTTGCCCGCGATGACGGCCTGACAGTCGACAACTTTG
TTGAATGTTCTACCGCTATC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSQTFYKKGFLALAVATALGVSAFAQADVKIGVAGPMTGANAAFGEQYMKGAQAAADAVNAAGGVNGEKIVLVKGDDACE
PKQAVTVAKDLTNQKVAGVVGHFCSSSTIPASEIYDEAGIIAITPGSTNPQVTERGLSAMFRMCGRDDQQGIVAGDYIVD
VLKGKKVVVLHDKDTYGQGLADATKAQLEKRGVKPVLYEGLTRGEKDFSTIVTKIRGAGADVVYFGGLHPEAGPLVRQLR
EQGLKDVKFMSDDGIVTDELVTTAGGPQFVDGVLMTFGADPRLLPDSKTVVDAFRKAGTEPEGYTLYAYASVQALAAAFN
GAKSNKGEEAAAWLKKNPVKTVMGEKTWDSKGDLKVSDYVVYQWDKDGKYHQLEKQK

Sequences:

>Translated_377_residues
MSQTFYKKGFLALAVATALGVSAFAQADVKIGVAGPMTGANAAFGEQYMKGAQAAADAVNAAGGVNGEKIVLVKGDDACE
PKQAVTVAKDLTNQKVAGVVGHFCSSSTIPASEIYDEAGIIAITPGSTNPQVTERGLSAMFRMCGRDDQQGIVAGDYIVD
VLKGKKVVVLHDKDTYGQGLADATKAQLEKRGVKPVLYEGLTRGEKDFSTIVTKIRGAGADVVYFGGLHPEAGPLVRQLR
EQGLKDVKFMSDDGIVTDELVTTAGGPQFVDGVLMTFGADPRLLPDSKTVVDAFRKAGTEPEGYTLYAYASVQALAAAFN
GAKSNKGEEAAAWLKKNPVKTVMGEKTWDSKGDLKVSDYVVYQWDKDGKYHQLEKQK
>Mature_376_residues
SQTFYKKGFLALAVATALGVSAFAQADVKIGVAGPMTGANAAFGEQYMKGAQAAADAVNAAGGVNGEKIVLVKGDDACEP
KQAVTVAKDLTNQKVAGVVGHFCSSSTIPASEIYDEAGIIAITPGSTNPQVTERGLSAMFRMCGRDDQQGIVAGDYIVDV
LKGKKVVVLHDKDTYGQGLADATKAQLEKRGVKPVLYEGLTRGEKDFSTIVTKIRGAGADVVYFGGLHPEAGPLVRQLRE
QGLKDVKFMSDDGIVTDELVTTAGGPQFVDGVLMTFGADPRLLPDSKTVVDAFRKAGTEPEGYTLYAYASVQALAAAFNG
AKSNKGEEAAAWLKKNPVKTVMGEKTWDSKGDLKVSDYVVYQWDKDGKYHQLEKQK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=362, Percent_Identity=41.9889502762431, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI1789867, Length=362, Percent_Identity=40.0552486187845, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39946; Mature: 39815

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTFYKKGFLALAVATALGVSAFAQADVKIGVAGPMTGANAAFGEQYMKGAQAAADAVN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AAGGVNGEKIVLVKGDDACEPKQAVTVAKDLTNQKVAGVVGHFCSSSTIPASEIYDEAGI
HCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCE
IAITPGSTNPQVTERGLSAMFRMCGRDDQQGIVAGDYIVDVLKGKKVVVLHDKDTYGQGL
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEEECCCCCCCCH
ADATKAQLEKRGVKPVLYEGLTRGEKDFSTIVTKIRGAGADVVYFGGLHPEAGPLVRQLR
HHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
EQGLKDVKFMSDDGIVTDELVTTAGGPQFVDGVLMTFGADPRLLPDSKTVVDAFRKAGTE
HCCCHHEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
PEGYTLYAYASVQALAAAFNGAKSNKGEEAAAWLKKNPVKTVMGEKTWDSKGDLKVSDYV
CCCEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEE
VYQWDKDGKYHQLEKQK
EEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SQTFYKKGFLALAVATALGVSAFAQADVKIGVAGPMTGANAAFGEQYMKGAQAAADAVN
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
AAGGVNGEKIVLVKGDDACEPKQAVTVAKDLTNQKVAGVVGHFCSSSTIPASEIYDEAGI
HCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHCCCCE
IAITPGSTNPQVTERGLSAMFRMCGRDDQQGIVAGDYIVDVLKGKKVVVLHDKDTYGQGL
EEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEEECCCCCCCCH
ADATKAQLEKRGVKPVLYEGLTRGEKDFSTIVTKIRGAGADVVYFGGLHPEAGPLVRQLR
HHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHH
EQGLKDVKFMSDDGIVTDELVTTAGGPQFVDGVLMTFGADPRLLPDSKTVVDAFRKAGTE
HCCCHHEEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCC
PEGYTLYAYASVQALAAAFNGAKSNKGEEAAAWLKKNPVKTVMGEKTWDSKGDLKVSDYV
CCCEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEE
VYQWDKDGKYHQLEKQK
EEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA