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The map label for this gene is puuB [H]

Identifier: 77456779

GI number: 77456779

Start: 644096

End: 645379

Strand: Direct

Name: puuB [H]

Synonym: Pfl01_0551

Alternate gene names: 77456779

Gene position: 644096-645379 (Clockwise)

Preceding gene: 77456778

Following gene: 77456780

Centisome position: 10.0

GC content: 63.79

Gene sequence:

>1284_bases
ATGCCGTTACGCGAAGAATGTCTGTGGGAAAAACTCACGCCGCAACGACCGGATAACACGGCGCTCAGGGGCGAGGTCAA
AGTCGATGTCTGCGTGATCGGCGCCGGGTTTACCGGGCTGTCGGCGGCGCTGCATCTGCTGGAGAAAGGCAAAAGTGTCT
GCGTGCTCGAAGCCCATCGCGCCGGGCATGGTGGCTCCGGGCGCAACGTCGGGCTGGTCAACGCCGGGATGTGGATTCCG
CCGGACGAGATAGAGGCCGGCTTCGGCGAGGCGGTCGGCAGTCAGCTCAACCGCATGCTCGGTGCGGCGCCGGCGCTGGT
GTTCAGCCTTGTGGATAAATACAACATCGATTGCCAGTTGCGCCGCGAAGGCACCTTGCACATGGCGCACAACGCCAGGG
GTGAGGCGGACCTGCGCAGTCGCGAAGAACAATGGAAACGCCGCGGCGCACCGGTCGAACTGCTGACCGGCAAGGCCTGT
GAACAGGCGACCGGCACGCAGAAAATTGCTGCCGCGCTGCTTGATCGGCGTGCCGGTACGCTCAATCCGATGGCGTATGT
CACCGGGCTGGCCAACGCTGTAATGAGCCTTGGCGGGCAGATGTTCGATCACTCCCCGGTCGCGCGTCTCGAACGCCAGG
GGCCGAAATGGTCGGTGCAGACCGAGCAGGGTTCGGTGCTCGCCGAACAGGTGGTTATCGCTTCCAACGCCTACACCGAA
GGCGACTGGACCGAACTCAAGCGCAATTTCTTCCCCGGTTACTACTATCAGGTCGCTTCGGTGCCGCTCACCGAAGACGC
CGCTCAGGAAATCCTCCCGGGCGGGCAGGGCTCGTGGGATACCCGTCAGGTGCTGAGCAGCATCCGCCGTGACAAGGATG
GTCGTTTGCTGCTGGGCAGTCTCGGCAACGGCAATCAGAAACCGACCTGGTTCCTCAAGGCCTGGGCCGACCGGGTGCAG
CAGCATTACTTCCCCAACCTCAAACCCGTGGAGTGGGAATGCACCTGGACCGGGCGCATCGCGTTCACTCCCGATCACCT
GATGCGCCTGTTCGAGCCGGCGCCGGGGCTAGTGGCCGTCACCGGTTACAACGGCCGTGGCGTGACCACCGGCACCGTGG
TCGGCAAGGCCTTCGCCGATTACCTGTGTAACGGAAATCCTCAGGCATTGCCGATTCCGTTCGCACCGATGCAGCCGCTG
GCGGGGGTGGGCCTGCGCAGTTGTCTGTACGAGGCCGGGTTCTCGCTGTATCACGCGGGCCAGTGCCTGCGGATCGTCAT
CTGA

Upstream 100 bases:

>100_bases
TGTTGGCTGAAACGCCGCTTTCGTCGGAGCGCCGCCCGGAGCAGGCTCGCTTCCACATTGTTGAACTGTGTGTTTGTTAG
GTTTCTGTTGGAGTCTGGCA

Downstream 100 bases:

>100_bases
GTGTTGAAATATGTTGCCGGAAGCGGCGCTTTTCGATGTAGCGACTAGCCTGTAATGGTGCGGGTTGTAGCAGTCCCGCA
CCAGCAGTGTGCAGTTCGGT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPLREECLWEKLTPQRPDNTALRGEVKVDVCVIGAGFTGLSAALHLLEKGKSVCVLEAHRAGHGGSGRNVGLVNAGMWIP
PDEIEAGFGEAVGSQLNRMLGAAPALVFSLVDKYNIDCQLRREGTLHMAHNARGEADLRSREEQWKRRGAPVELLTGKAC
EQATGTQKIAAALLDRRAGTLNPMAYVTGLANAVMSLGGQMFDHSPVARLERQGPKWSVQTEQGSVLAEQVVIASNAYTE
GDWTELKRNFFPGYYYQVASVPLTEDAAQEILPGGQGSWDTRQVLSSIRRDKDGRLLLGSLGNGNQKPTWFLKAWADRVQ
QHYFPNLKPVEWECTWTGRIAFTPDHLMRLFEPAPGLVAVTGYNGRGVTTGTVVGKAFADYLCNGNPQALPIPFAPMQPL
AGVGLRSCLYEAGFSLYHAGQCLRIVI

Sequences:

>Translated_427_residues
MPLREECLWEKLTPQRPDNTALRGEVKVDVCVIGAGFTGLSAALHLLEKGKSVCVLEAHRAGHGGSGRNVGLVNAGMWIP
PDEIEAGFGEAVGSQLNRMLGAAPALVFSLVDKYNIDCQLRREGTLHMAHNARGEADLRSREEQWKRRGAPVELLTGKAC
EQATGTQKIAAALLDRRAGTLNPMAYVTGLANAVMSLGGQMFDHSPVARLERQGPKWSVQTEQGSVLAEQVVIASNAYTE
GDWTELKRNFFPGYYYQVASVPLTEDAAQEILPGGQGSWDTRQVLSSIRRDKDGRLLLGSLGNGNQKPTWFLKAWADRVQ
QHYFPNLKPVEWECTWTGRIAFTPDHLMRLFEPAPGLVAVTGYNGRGVTTGTVVGKAFADYLCNGNPQALPIPFAPMQPL
AGVGLRSCLYEAGFSLYHAGQCLRIVI
>Mature_426_residues
PLREECLWEKLTPQRPDNTALRGEVKVDVCVIGAGFTGLSAALHLLEKGKSVCVLEAHRAGHGGSGRNVGLVNAGMWIPP
DEIEAGFGEAVGSQLNRMLGAAPALVFSLVDKYNIDCQLRREGTLHMAHNARGEADLRSREEQWKRRGAPVELLTGKACE
QATGTQKIAAALLDRRAGTLNPMAYVTGLANAVMSLGGQMFDHSPVARLERQGPKWSVQTEQGSVLAEQVVIASNAYTEG
DWTELKRNFFPGYYYQVASVPLTEDAAQEILPGGQGSWDTRQVLSSIRRDKDGRLLLGSLGNGNQKPTWFLKAWADRVQQ
HYFPNLKPVEWECTWTGRIAFTPDHLMRLFEPAPGLVAVTGYNGRGVTTGTVVGKAFADYLCNGNPQALPIPFAPMQPLA
GVGLRSCLYEAGFSLYHAGQCLRIVI

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=385, Percent_Identity=31.1688311688312, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46511; Mature: 46379

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLREECLWEKLTPQRPDNTALRGEVKVDVCVIGAGFTGLSAALHLLEKGKSVCVLEAHR
CCCHHHHHHHHCCCCCCCCCCEECEEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECC
AGHGGSGRNVGLVNAGMWIPPDEIEAGFGEAVGSQLNRMLGAAPALVFSLVDKYNIDCQL
CCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
RREGTLHMAHNARGEADLRSREEQWKRRGAPVELLTGKACEQATGTQKIAAALLDRRAGT
ECCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHCCHHHHHHHHHHHCCCC
LNPMAYVTGLANAVMSLGGQMFDHSPVARLERQGPKWSVQTEQGSVLAEQVVIASNAYTE
CCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHCCCCEEEECCCCCHHHHHHEEECCCCCC
GDWTELKRNFFPGYYYQVASVPLTEDAAQEILPGGQGSWDTRQVLSSIRRDKDGRLLLGS
CCHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
LGNGNQKPTWFLKAWADRVQQHYFPNLKPVEWECTWTGRIAFTPDHLMRLFEPAPGLVAV
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECEEEECHHHHHHHHCCCCCEEEE
TGYNGRGVTTGTVVGKAFADYLCNGNPQALPIPFAPMQPLAGVGLRSCLYEAGFSLYHAG
ECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCEECC
QCLRIVI
CEEEEEC
>Mature Secondary Structure 
PLREECLWEKLTPQRPDNTALRGEVKVDVCVIGAGFTGLSAALHLLEKGKSVCVLEAHR
CCHHHHHHHHCCCCCCCCCCEECEEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECC
AGHGGSGRNVGLVNAGMWIPPDEIEAGFGEAVGSQLNRMLGAAPALVFSLVDKYNIDCQL
CCCCCCCCEEEEEECCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
RREGTLHMAHNARGEADLRSREEQWKRRGAPVELLTGKACEQATGTQKIAAALLDRRAGT
ECCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHCCHHHHHHHHHHHCCCC
LNPMAYVTGLANAVMSLGGQMFDHSPVARLERQGPKWSVQTEQGSVLAEQVVIASNAYTE
CCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHCCCCEEEECCCCCHHHHHHEEECCCCCC
GDWTELKRNFFPGYYYQVASVPLTEDAAQEILPGGQGSWDTRQVLSSIRRDKDGRLLLGS
CCHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
LGNGNQKPTWFLKAWADRVQQHYFPNLKPVEWECTWTGRIAFTPDHLMRLFEPAPGLVAV
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECEEEECHHHHHHHHCCCCCEEEE
TGYNGRGVTTGTVVGKAFADYLCNGNPQALPIPFAPMQPLAGVGLRSCLYEAGFSLYHAG
ECCCCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCEECC
QCLRIVI
CEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]