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The map label for this gene is nadE

Identifier: 77456775

GI number: 77456775

Start: 639208

End: 640035

Strand: Reverse

Name: nadE

Synonym: Pfl01_0547

Alternate gene names: 77456775

Gene position: 640035-639208 (Counterclockwise)

Preceding gene: 77456776

Following gene: 77456773

Centisome position: 9.94

GC content: 63.89

Gene sequence:

>828_bases
ATGCAAGCCGTACAGCGTGAGATTGCTGAACAGCTCAACGTTCAGCCGCCGTTCGCCGATGACCAGGCCCTCGAAGCGGA
AGTCGCCCGCCGCATCAGCTTCATCCAGGATTGCCTGACCAGTTCCGGACTCAAGACCCTGGTGCTGGGCATCAGCGGCG
GGGTCGATTCCCTGACCGCCGGCCTTCTGGCCCAGCGTGCGATGCGCGAGTTGCGCGAGCGCACTGGTGACGAGGCCTAC
AAGTTCATCGCCGTGCGCCTGCCGTATGACGTGCAGTTCGATGAACACGACGCCCAGGCCTCGGTGGACTTCATCGCCCC
GGACGAACGCCACACCGTCAACATCGGCCCGGCGGTCAAATCCCTGGCCAGCGAAGTCGCAGCCTTCGAAGGCAAGCACG
CGGTGTCGGTGGACTTCGTGCTCGGCAACACCAAGGCGCGGATGCGCATGGTCGCCCAGTACACGATCGCCGGCGCGACT
CATGGTCTGGTGATCGGCACCGACCACGCGGCGGAAGCGGTGATGGGTTTCTTCACCAAGTTCGGTGACGGCGCCTGCGA
TCTGGCCCCGCTCAGCGGTCTGGTGAAAAACCAGGTCCGGGCCATCGCCCGCAGCTTCGGCGCACCGGAATCACTGGTGG
AAAAAGTGCCGACGGCGGATCTGGAAGACCTGTCGCCGGGCAAGCCGGACGAGGCGTCACACGGCGTGACCTACGCCGAG
ATCGACGCTTTCCTGCACGGAGAGCCGGTGCGTCAGGAGGCGTTCGACATCATCGTCAACACCTACAAAAAGACTCATCA
CAAGCGGGTCATGCCGTTTGCGCCTTGA

Upstream 100 bases:

>100_bases
CCGGGCAAGACCCACTGCAAAGACCCGAATTTCGTCGCCTATTTGCGACACGTTTTCCAGGTTCCTGCCGTTTCCAGCCT
TTCCAGCAAGGAGTGAATTC

Downstream 100 bases:

>100_bases
ATCCAGGGCATAAAAAAAGCGTCCTTCGGGACGCTTTTTTTTGACCTTGTGTTGCTTACTTCAGGGTTACGGTGCCTTTC
ATCATCGAGATGTGGCCCGG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MQAVQREIAEQLNVQPPFADDQALEAEVARRISFIQDCLTSSGLKTLVLGISGGVDSLTAGLLAQRAMRELRERTGDEAY
KFIAVRLPYDVQFDEHDAQASVDFIAPDERHTVNIGPAVKSLASEVAAFEGKHAVSVDFVLGNTKARMRMVAQYTIAGAT
HGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARSFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
IDAFLHGEPVRQEAFDIIVNTYKKTHHKRVMPFAP

Sequences:

>Translated_275_residues
MQAVQREIAEQLNVQPPFADDQALEAEVARRISFIQDCLTSSGLKTLVLGISGGVDSLTAGLLAQRAMRELRERTGDEAY
KFIAVRLPYDVQFDEHDAQASVDFIAPDERHTVNIGPAVKSLASEVAAFEGKHAVSVDFVLGNTKARMRMVAQYTIAGAT
HGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARSFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
IDAFLHGEPVRQEAFDIIVNTYKKTHHKRVMPFAP
>Mature_275_residues
MQAVQREIAEQLNVQPPFADDQALEAEVARRISFIQDCLTSSGLKTLVLGISGGVDSLTAGLLAQRAMRELRERTGDEAY
KFIAVRLPYDVQFDEHDAQASVDFIAPDERHTVNIGPAVKSLASEVAAFEGKHAVSVDFVLGNTKARMRMVAQYTIAGAT
HGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARSFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
IDAFLHGEPVRQEAFDIIVNTYKKTHHKRVMPFAP

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Homo sapiens, GI41393551, Length=158, Percent_Identity=30.379746835443, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788036, Length=271, Percent_Identity=50.9225092250922, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_PSEPF (Q3KIW5)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346280.1
- HSSP:   P18843
- ProteinModelPortal:   Q3KIW5
- SMR:   Q3KIW5
- STRING:   Q3KIW5
- World-2DPAGE:   0008:Q3KIW5
- GeneID:   3712942
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_0547
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   PELEWAM
- ProtClustDB:   PRK00768
- BioCyc:   PFLU205922:PFL_0547-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 29745; Mature: 29745

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVQREIAEQLNVQPPFADDQALEAEVARRISFIQDCLTSSGLKTLVLGISGGVDSLTA
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHH
GLLAQRAMRELRERTGDEAYKFIAVRLPYDVQFDEHDAQASVDFIAPDERHTVNIGPAVK
HHHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECCHHHH
SLASEVAAFEGKHAVSVDFVLGNTKARMRMVAQYTIAGATHGLVIGTDHAAEAVMGFFTK
HHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
FGDGACDLAPLSGLVKNQVRAIARSFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
IDAFLHGEPVRQEAFDIIVNTYKKTHHKRVMPFAP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQAVQREIAEQLNVQPPFADDQALEAEVARRISFIQDCLTSSGLKTLVLGISGGVDSLTA
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHH
GLLAQRAMRELRERTGDEAYKFIAVRLPYDVQFDEHDAQASVDFIAPDERHTVNIGPAVK
HHHHHHHHHHHHHHCCCHHEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEEECCHHHH
SLASEVAAFEGKHAVSVDFVLGNTKARMRMVAQYTIAGATHGLVIGTDHAAEAVMGFFTK
HHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
FGDGACDLAPLSGLVKNQVRAIARSFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
IDAFLHGEPVRQEAFDIIVNTYKKTHHKRVMPFAP
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA