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The map label for this gene is hldE [H]

Identifier: 77456712

GI number: 77456712

Start: 560968

End: 562392

Strand: Direct

Name: hldE [H]

Synonym: Pfl01_0484

Alternate gene names: 77456712

Gene position: 560968-562392 (Clockwise)

Preceding gene: 77456711

Following gene: 77456717

Centisome position: 8.71

GC content: 64.28

Gene sequence:

>1425_bases
ATGAAGTTGTCCATGCCGCGATTCGATCAAGCCCCTGTCTTGGTGGTCGGCGATGTCATGCTCGACCGTTACTGGCATGG
TGGTACCTCACGGATTTCCCCTGAGGCGCCGGTTCCTGTCGTTAAAGTCGAGCAAATCGAAGACCGCCCGGGCGGTGCCG
CCAACGTTGCCTTGAACATTGCCGCGCTGGGTGCGCCGGCGTCGCTGGTCGGTGTGACTGGTGACGACGAAGCCGCCGAC
AGCCTGAGCAACAGCCTCAAGGGTGCGGGCGTACGAGCCTTGTTCCAGCGCATCGCGCACCAGCCGACCATCGTCAAGCT
GCGGGTCATGAGCCGGCACCAGCAATTGCTGCGTATCGACTTCGAAGAACCGTTCGCCACCGATGCACTGGCCCTTGGCG
CGCAGGTCGACGACCTGCTCGAAGGCATCAAGGTGCTGGTGCTGTCCGACTACGGCAAAGGCGCCCTGAAAAACCATCAG
GCCTTGATCCAGGCCGCCCGTGCCAAAGGCATTCCGGTGCTGGCCGACCCGAAGGGCAAGGATTTCTCGATCTACCGTGG
TGCCAGCCTGATCACCCCGAACCTCAGCGAGTTCGAAGCCATCGTCGGCGGTTGCGCCGATGAGCACGAGCTGGTGACCA
AGGGCGCGACGCTGATGCACGACCTGGATCTCGGCGCACTGCTGGTGACCCGTGGCGAACACGGCATGACCCTGCTGCGT
CCGGATCATCCGGCGCTGCATTTGCCTGCGCGGGCCCGTGAAGTGTTCGACGTGACCGGTGCCGGCGACACGGTGATTTC
CACCCTGGCAGCCGCGATTGCTGCCGGTGAAGAATTGCCGCACGCGGTGGCGCTGGCCAACTTGGCGGCGGGCATCGTGG
TCGGCAAGTTGGGTACCGCGGCGATCAGCGCCCCGGAACTGCGTCGCGCCATTCAGCGTGAAGAAGGTTCCGAGCGTGGC
GTGCTGGGGCTGGAGCAACTGCTGCTGGCAGTCGCCGATGCCCGTGCGCACAATGAGAAGATCGTGTTCACCAACGGCTG
CTTCGACATCCTGCACGCCGGCCACGTGACCTACCTCGAGCAGGCGCGAGCCCAGGGCGATCGCCTGATCGTCGCGATCA
ACGACGACGCCTCGGTCAGCCGCCTGAAAGGGCCGGGCCGGCCGATCAACAGCGTCGACCGGCGCATGGCGGTTCTCGCC
GGTCTCGGTGCGGTGGACTGGGTCATCAGCTTTGCCGAAGGCACCCCGGAAAACCTGCTGCGCGAGGTCAAGCCGGACGT
GCTGGTGAAGGGTGGCGATTACGGCATCGACCAGGTTGTCGGCGCTGATATCGTCAAAGCCTACGGCGGCGCCGTCAAAG
TGCTGGGGCTTGTGGAGAACAGCTCGACCACGGCGATTGTGGAAAAGATCCGCAAGTCGGAGTGA

Upstream 100 bases:

>100_bases
AAAAAAGCCAGCTGTTTCATCCTGTTGCAGCCACCGCACAAGCCTTCGCCAACCCTGTGTTAATATCGCGCCCCTGTTCA
TTTTGTATGTGGGTTGCTCC

Downstream 100 bases:

>100_bases
TGCAAACATAAAGACCGCGTCTGCGATGACTCTCCGGAGTCACTCGCAGGCGCGGTCTTTTGCCGTTCAGGAATTCACTT
TCTTGCGCGGCACGATCTTC

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MKLSMPRFDQAPVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVEQIEDRPGGAANVALNIAALGAPASLVGVTGDDEAAD
SLSNSLKGAGVRALFQRIAHQPTIVKLRVMSRHQQLLRIDFEEPFATDALALGAQVDDLLEGIKVLVLSDYGKGALKNHQ
ALIQAARAKGIPVLADPKGKDFSIYRGASLITPNLSEFEAIVGGCADEHELVTKGATLMHDLDLGALLVTRGEHGMTLLR
PDHPALHLPARAREVFDVTGAGDTVISTLAAAIAAGEELPHAVALANLAAGIVVGKLGTAAISAPELRRAIQREEGSERG
VLGLEQLLLAVADARAHNEKIVFTNGCFDILHAGHVTYLEQARAQGDRLIVAINDDASVSRLKGPGRPINSVDRRMAVLA
GLGAVDWVISFAEGTPENLLREVKPDVLVKGGDYGIDQVVGADIVKAYGGAVKVLGLVENSSTTAIVEKIRKSE

Sequences:

>Translated_474_residues
MKLSMPRFDQAPVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVEQIEDRPGGAANVALNIAALGAPASLVGVTGDDEAAD
SLSNSLKGAGVRALFQRIAHQPTIVKLRVMSRHQQLLRIDFEEPFATDALALGAQVDDLLEGIKVLVLSDYGKGALKNHQ
ALIQAARAKGIPVLADPKGKDFSIYRGASLITPNLSEFEAIVGGCADEHELVTKGATLMHDLDLGALLVTRGEHGMTLLR
PDHPALHLPARAREVFDVTGAGDTVISTLAAAIAAGEELPHAVALANLAAGIVVGKLGTAAISAPELRRAIQREEGSERG
VLGLEQLLLAVADARAHNEKIVFTNGCFDILHAGHVTYLEQARAQGDRLIVAINDDASVSRLKGPGRPINSVDRRMAVLA
GLGAVDWVISFAEGTPENLLREVKPDVLVKGGDYGIDQVVGADIVKAYGGAVKVLGLVENSSTTAIVEKIRKSE
>Mature_474_residues
MKLSMPRFDQAPVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVEQIEDRPGGAANVALNIAALGAPASLVGVTGDDEAAD
SLSNSLKGAGVRALFQRIAHQPTIVKLRVMSRHQQLLRIDFEEPFATDALALGAQVDDLLEGIKVLVLSDYGKGALKNHQ
ALIQAARAKGIPVLADPKGKDFSIYRGASLITPNLSEFEAIVGGCADEHELVTKGATLMHDLDLGALLVTRGEHGMTLLR
PDHPALHLPARAREVFDVTGAGDTVISTLAAAIAAGEELPHAVALANLAAGIVVGKLGTAAISAPELRRAIQREEGSERG
VLGLEQLLLAVADARAHNEKIVFTNGCFDILHAGHVTYLEQARAQGDRLIVAINDDASVSRLKGPGRPINSVDRRMAVLA
GLGAVDWVISFAEGTPENLLREVKPDVLVKGGDYGIDQVVGADIVKAYGGAVKVLGLVENSSTTAIVEKIRKSE

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=475, Percent_Identity=57.6842105263158, Blast_Score=536, Evalue=1e-153,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 49976; Mature: 49976

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSMPRFDQAPVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVEQIEDRPGGAANVALNI
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEE
AALGAPASLVGVTGDDEAADSLSNSLKGAGVRALFQRIAHQPTIVKLRVMSRHQQLLRID
EEECCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHEEC
FEEPFATDALALGAQVDDLLEGIKVLVLSDYGKGALKNHQALIQAARAKGIPVLADPKGK
CCCCCCHHHHHHCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCC
DFSIYRGASLITPNLSEFEAIVGGCADEHELVTKGATLMHDLDLGALLVTRGEHGMTLLR
CEEEECCCEEECCCHHHHHHHHCCCCCCHHHHHCCCHHEEECCCCEEEEECCCCCEEEEC
PDHPALHLPARAREVFDVTGAGDTVISTLAAAIAAGEELPHAVALANLAAGIVVGKLGTA
CCCCCEECCHHHHHHEEECCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCHH
AISAPELRRAIQREEGSERGVLGLEQLLLAVADARAHNEKIVFTNGCFDILHAGHVTYLE
HCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHH
QARAQGDRLIVAINDDASVSRLKGPGRPINSVDRRMAVLAGLGAVDWVISFAEGTPENLL
HHHCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHH
REVKPDVLVKGGDYGIDQVVGADIVKAYGGAVKVLGLVENSSTTAIVEKIRKSE
HHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MKLSMPRFDQAPVLVVGDVMLDRYWHGGTSRISPEAPVPVVKVEQIEDRPGGAANVALNI
CCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEE
AALGAPASLVGVTGDDEAADSLSNSLKGAGVRALFQRIAHQPTIVKLRVMSRHQQLLRID
EEECCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHEEC
FEEPFATDALALGAQVDDLLEGIKVLVLSDYGKGALKNHQALIQAARAKGIPVLADPKGK
CCCCCCHHHHHHCCCHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCC
DFSIYRGASLITPNLSEFEAIVGGCADEHELVTKGATLMHDLDLGALLVTRGEHGMTLLR
CEEEECCCEEECCCHHHHHHHHCCCCCCHHHHHCCCHHEEECCCCEEEEECCCCCEEEEC
PDHPALHLPARAREVFDVTGAGDTVISTLAAAIAAGEELPHAVALANLAAGIVVGKLGTA
CCCCCEECCHHHHHHEEECCCCHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHCCCHH
AISAPELRRAIQREEGSERGVLGLEQLLLAVADARAHNEKIVFTNGCFDILHAGHVTYLE
HCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCHHHHHH
QARAQGDRLIVAINDDASVSRLKGPGRPINSVDRRMAVLAGLGAVDWVISFAEGTPENLL
HHHCCCCEEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHH
REVKPDVLVKGGDYGIDQVVGADIVKAYGGAVKVLGLVENSSTTAIVEKIRKSE
HHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA