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The map label for this gene is mrcA [H]

Identifier: 77456632

GI number: 77456632

Start: 459017

End: 461464

Strand: Reverse

Name: mrcA [H]

Synonym: Pfl01_0404

Alternate gene names: 77456632

Gene position: 461464-459017 (Counterclockwise)

Preceding gene: 77456644

Following gene: 77456628

Centisome position: 7.17

GC content: 62.62

Gene sequence:

>2448_bases
TTGATTCGTCTGCTGAAATTTTTCGGTTGGTCCATCGTCGCCGTTTTCTGCGGACTGCTTTTAGGTCTCAGCGGCGCGTT
TCTTTACCTTAGTCCGGGTTTGCCATCTGTGGAGGCGCTGAGAAGCATTCAGTTGCAGATTCCGCTCCGGGTCTACAGCA
GCGACAACAAGTTGATCGCAGAATTTGGCGAAATGCGCCGCACACCGATCCGTTTCGCCGACATTCCCCCCAATTTCATC
AATGCGTTACTAAGTGCTGAAGACGACAACTTCGCCAACCACTACGGCGTTGATCCGAGCAGCCTGATGCGTGCCGCGAC
CCAACTGGTCAAGAGCGGGCACATCCAGTCCGGCGGCAGCACCATCACCATGCAGGTGGCGAAGAACTTCTTCCTGACCA
GCGAGCGCAGCTTCTCGCGCAAGACCACTGAAATCCTTCTCGCCCTGCAGATCGAACGCCAACTGACCAAGGACGAGATC
CTTGAGCTGTACGTGAACAAGATCTATCTGGGCAACCGCGCCTACGGCATCGAGGCGGCGGCACAGGTGTATTACGGCAA
GTCGATCCGCGAAGTCAGCCTGGCGCAGATGGCGATGATCGCCGGCCTGCCGAAAGCCCCGTCGCGCTTCAACCCGCTGG
CCAACCCGGCGCGCAGCAAGGAGCGTCGTGACTGGATCCTTGGCCGCATGTACAAGCTCGGCAAGATCACCGAAGCCGAC
TACACCGCCGCGATCAACGAGCCGCTGAATGCCAGCTATCACGTGCCGACCCCGGAAGTGAACGCGCCGTACATCGCCGA
GATGGCCCGTGCGGAAATGGTCGGTCGCTACGGCAGCGACGCCTATACCGAAGGTTTCCGCGTTACCACCACGGTGCCGA
GCAACCTCCAGGAAATGGCCAACACCGCGCTGCATGAAGGCCTGATGACCTACGACCAGCGTCACGGCTACCGTGGCCCT
GAATCACGCCTGCCGGGCAAGACCCGTGAAGCCTGGGCCACCGAACTGACCAAGCAACGCACCATCAGCAGCCTCGAGCC
GGCCATCGTCACCCAGGTCGACAAGACCGGCCTGCAAGTGCTGACCCGCACCGGCGAAGAGCATGTGGCGTGGGACACCA
TGAAATGGGCGCGACCGTTCCTCAACACCAACAGCATGGGCGCCGCGCCGCGTCAGCCGTCGGATGTGGCACAGATCGGC
GACCTGATTCGCGTGCAGCGCCAGCCGAACAATTCGCTGAAGTTCAGCCAGATTCCGCAGGCGCAAGGCGCGCTGGTGTC
ACTGGATCCGCAGAACGGCGCGATCCGTTCGCTGGTCGGTGGTTTCGCCTTCGAGCAGAGCAACTACAACCGCGCGATGC
AGGCCAAGCGTCAGCCGGGCTCGAGCTTCAAGCCGTTCGTCTACAGCGCCGCGCTGGATAACGGCTACACCGCCGCCAGC
CTGGTCAACGACGCACCGATCGTGTTCGTCGACGAGTACCTGGACAAGGTCTGGCGTCCGAAGAACGACACCAACACTTT
CCTCGGCCCGATCCGCCTGCGTGAGGCGCTGTACAAGTCGCGCAACCTGGTGTCGATCCGCCTGTTGCAGGCGATGGGCG
TAGGCAAGACCATCGACTACATCACCCGCTTCGGCTTCAACAAGCAGGACCTGCCGCCAAACCTGTCGCTGGCGCTGGGC
ACCGCGACCCTGACGCCGATGGAAATCGCCACTGGCTGGAGCACCTTTGCCAACGGCGGCTACAAGATCACCCCGTACAT
CATCGACAAGATCGAAAGCCGCAATGGCGATACTCTCTTCGTCGCCAACCCGCCAACCGTACCGCAGGGCGCCGCAGCTA
CCGACGGCATTGCCGCACCCGCCACCCAGGCGTTTACCGTCAATGTAGCGCCGGTTCCGGGTGAAGCGCCGGGCAACGCT
GCCGTGCCCCAAGCACCAGCCGTCGCCGAGCGCATCGTCGATGGCCGTACCACCTACATCCTCAACAGCATGCTGCAGGA
CGTGATCAAGCTTGGCACCGGCCGGCGCGCACTGGCTCTGGGCCGCAGCGACATCGCCGGCAAGACCGGTACCACCAACG
AATCCAAGGATGCGTGGTTCTCCGGCTACAACGCCGACTACGTGACCACCGTGTGGACCGGTTTCGACCAGCCGGAAAGC
CTCGGTCGCCGGGAATTCGGGGGCACCGTGGCACTGCCGATCTGGATGAACTACATGGCCGCCGCGCTCAAGGACAAGCC
ACCGCATGTCCAGCCGGAGCCGGAAGGCATCCTCAGCCTGCGGGTGGATCCGGTGAGCGGTCGTGCGGCATCGCCGAGCA
CGCCGGGTGCGTACTTCGAGCTGTTCAAGGCTGAGGACACGCCGCCGTCGGTCAACGAGATCGGCAATGGCGCCGTACCG
GGCAGTCCGCTGCCGGCGGATGAACAGGCGCCGATCGATTTGTTCTGA

Upstream 100 bases:

>100_bases
CAGCGTTTTTTTCCGCTTTTTACTGCCGGCGCGGGTCGTTCTGTGTGTTAGCCGGGACCCTGTCGCCCAATTCATTCTTT
GCCGTGGATGTCCAAAAGCC

Downstream 100 bases:

>100_bases
TCCTGATCCGCCAAAAAGCCCCGCCTTCGATTGAAGTGCGGGGCTTTTTATTACCTGTTCATTTTTGTGTTGTCTGGGCC
GGCCTCTTCGCGAGCAAGCT

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MIRLLKFFGWSIVAVFCGLLLGLSGAFLYLSPGLPSVEALRSIQLQIPLRVYSSDNKLIAEFGEMRRTPIRFADIPPNFI
NALLSAEDDNFANHYGVDPSSLMRAATQLVKSGHIQSGGSTITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEI
LELYVNKIYLGNRAYGIEAAAQVYYGKSIREVSLAQMAMIAGLPKAPSRFNPLANPARSKERRDWILGRMYKLGKITEAD
YTAAINEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSDAYTEGFRVTTTVPSNLQEMANTALHEGLMTYDQRHGYRGP
ESRLPGKTREAWATELTKQRTISSLEPAIVTQVDKTGLQVLTRTGEEHVAWDTMKWARPFLNTNSMGAAPRQPSDVAQIG
DLIRVQRQPNNSLKFSQIPQAQGALVSLDPQNGAIRSLVGGFAFEQSNYNRAMQAKRQPGSSFKPFVYSAALDNGYTAAS
LVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQAMGVGKTIDYITRFGFNKQDLPPNLSLALG
TATLTPMEIATGWSTFANGGYKITPYIIDKIESRNGDTLFVANPPTVPQGAAATDGIAAPATQAFTVNVAPVPGEAPGNA
AVPQAPAVAERIVDGRTTYILNSMLQDVIKLGTGRRALALGRSDIAGKTGTTNESKDAWFSGYNADYVTTVWTGFDQPES
LGRREFGGTVALPIWMNYMAAALKDKPPHVQPEPEGILSLRVDPVSGRAASPSTPGAYFELFKAEDTPPSVNEIGNGAVP
GSPLPADEQAPIDLF

Sequences:

>Translated_815_residues
MIRLLKFFGWSIVAVFCGLLLGLSGAFLYLSPGLPSVEALRSIQLQIPLRVYSSDNKLIAEFGEMRRTPIRFADIPPNFI
NALLSAEDDNFANHYGVDPSSLMRAATQLVKSGHIQSGGSTITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEI
LELYVNKIYLGNRAYGIEAAAQVYYGKSIREVSLAQMAMIAGLPKAPSRFNPLANPARSKERRDWILGRMYKLGKITEAD
YTAAINEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSDAYTEGFRVTTTVPSNLQEMANTALHEGLMTYDQRHGYRGP
ESRLPGKTREAWATELTKQRTISSLEPAIVTQVDKTGLQVLTRTGEEHVAWDTMKWARPFLNTNSMGAAPRQPSDVAQIG
DLIRVQRQPNNSLKFSQIPQAQGALVSLDPQNGAIRSLVGGFAFEQSNYNRAMQAKRQPGSSFKPFVYSAALDNGYTAAS
LVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQAMGVGKTIDYITRFGFNKQDLPPNLSLALG
TATLTPMEIATGWSTFANGGYKITPYIIDKIESRNGDTLFVANPPTVPQGAAATDGIAAPATQAFTVNVAPVPGEAPGNA
AVPQAPAVAERIVDGRTTYILNSMLQDVIKLGTGRRALALGRSDIAGKTGTTNESKDAWFSGYNADYVTTVWTGFDQPES
LGRREFGGTVALPIWMNYMAAALKDKPPHVQPEPEGILSLRVDPVSGRAASPSTPGAYFELFKAEDTPPSVNEIGNGAVP
GSPLPADEQAPIDLF
>Mature_815_residues
MIRLLKFFGWSIVAVFCGLLLGLSGAFLYLSPGLPSVEALRSIQLQIPLRVYSSDNKLIAEFGEMRRTPIRFADIPPNFI
NALLSAEDDNFANHYGVDPSSLMRAATQLVKSGHIQSGGSTITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEI
LELYVNKIYLGNRAYGIEAAAQVYYGKSIREVSLAQMAMIAGLPKAPSRFNPLANPARSKERRDWILGRMYKLGKITEAD
YTAAINEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSDAYTEGFRVTTTVPSNLQEMANTALHEGLMTYDQRHGYRGP
ESRLPGKTREAWATELTKQRTISSLEPAIVTQVDKTGLQVLTRTGEEHVAWDTMKWARPFLNTNSMGAAPRQPSDVAQIG
DLIRVQRQPNNSLKFSQIPQAQGALVSLDPQNGAIRSLVGGFAFEQSNYNRAMQAKRQPGSSFKPFVYSAALDNGYTAAS
LVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQAMGVGKTIDYITRFGFNKQDLPPNLSLALG
TATLTPMEIATGWSTFANGGYKITPYIIDKIESRNGDTLFVANPPTVPQGAAATDGIAAPATQAFTVNVAPVPGEAPGNA
AVPQAPAVAERIVDGRTTYILNSMLQDVIKLGTGRRALALGRSDIAGKTGTTNESKDAWFSGYNADYVTTVWTGFDQPES
LGRREFGGTVALPIWMNYMAAALKDKPPHVQPEPEGILSLRVDPVSGRAASPSTPGAYFELFKAEDTPPSVNEIGNGAVP
GSPLPADEQAPIDLF

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=838, Percent_Identity=40.3341288782816, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1788867, Length=251, Percent_Identity=35.0597609561753, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1786343, Length=357, Percent_Identity=30.2521008403361, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1789601, Length=161, Percent_Identity=36.0248447204969, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 88974; Mature: 88974

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLLKFFGWSIVAVFCGLLLGLSGAFLYLSPGLPSVEALRSIQLQIPLRVYSSDNKLIA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHEEEEEEEEEECCCCHHHH
EFGEMRRTPIRFADIPPNFINALLSAEDDNFANHYGVDPSSLMRAATQLVKSGHIQSGGS
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
TITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEILELYVNKIYLGNRAYGIEAA
EEEEEEHHHHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHH
AQVYYGKSIREVSLAQMAMIAGLPKAPSRFNPLANPARSKERRDWILGRMYKLGKITEAD
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
YTAAINEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSDAYTEGFRVTTTVPSNLQEMA
HHHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEECCHHHHHHH
NTALHEGLMTYDQRHGYRGPESRLPGKTREAWATELTKQRTISSLEPAIVTQVDKTGLQV
HHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LTRTGEEHVAWDTMKWARPFLNTNSMGAAPRQPSDVAQIGDLIRVQRQPNNSLKFSQIPQ
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
AQGALVSLDPQNGAIRSLVGGFAFEQSNYNRAMQAKRQPGSSFKPFVYSAALDNGYTAAS
CCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEHHCCCCCHHH
LVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQAMGVGKTIDY
HCCCCCEEEHHHHHHHHCCCCCCCCCEECHHHHHHHHHHCCCEEEEEHHHHHCCCHHHHH
ITRFGFNKQDLPPNLSLALGTATLTPMEIATGWSTFANGGYKITPYIIDKIESRNGDTLF
HHHCCCCCCCCCCCCEEEECCCCCCCHHHHCCHHHHCCCCEEEEHHHHHHHHCCCCCEEE
VANPPTVPQGAAATDGIAAPATQAFTVNVAPVPGEAPGNAAVPQAPAVAERIVDGRTTYI
EECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHH
LNSMLQDVIKLGTGRRALALGRSDIAGKTGTTNESKDAWFSGYNADYVTTVWTGFDQPES
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCHHH
LGRREFGGTVALPIWMNYMAAALKDKPPHVQPEPEGILSLRVDPVSGRAASPSTPGAYFE
HHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHEE
LFKAEDTPPSVNEIGNGAVPGSPLPADEQAPIDLF
EEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIRLLKFFGWSIVAVFCGLLLGLSGAFLYLSPGLPSVEALRSIQLQIPLRVYSSDNKLIA
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHEEEEEEEEEECCCCHHHH
EFGEMRRTPIRFADIPPNFINALLSAEDDNFANHYGVDPSSLMRAATQLVKSGHIQSGGS
HHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHCCCCCCCCC
TITMQVAKNFFLTSERSFSRKTTEILLALQIERQLTKDEILELYVNKIYLGNRAYGIEAA
EEEEEEHHHHHEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCHHHH
AQVYYGKSIREVSLAQMAMIAGLPKAPSRFNPLANPARSKERRDWILGRMYKLGKITEAD
HHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
YTAAINEPLNASYHVPTPEVNAPYIAEMARAEMVGRYGSDAYTEGFRVTTTVPSNLQEMA
HHHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEECCHHHHHHH
NTALHEGLMTYDQRHGYRGPESRLPGKTREAWATELTKQRTISSLEPAIVTQVDKTGLQV
HHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LTRTGEEHVAWDTMKWARPFLNTNSMGAAPRQPSDVAQIGDLIRVQRQPNNSLKFSQIPQ
HHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
AQGALVSLDPQNGAIRSLVGGFAFEQSNYNRAMQAKRQPGSSFKPFVYSAALDNGYTAAS
CCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEEHHCCCCCHHH
LVNDAPIVFVDEYLDKVWRPKNDTNTFLGPIRLREALYKSRNLVSIRLLQAMGVGKTIDY
HCCCCCEEEHHHHHHHHCCCCCCCCCEECHHHHHHHHHHCCCEEEEEHHHHHCCCHHHHH
ITRFGFNKQDLPPNLSLALGTATLTPMEIATGWSTFANGGYKITPYIIDKIESRNGDTLF
HHHCCCCCCCCCCCCEEEECCCCCCCHHHHCCHHHHCCCCEEEEHHHHHHHHCCCCCEEE
VANPPTVPQGAAATDGIAAPATQAFTVNVAPVPGEAPGNAAVPQAPAVAERIVDGRTTYI
EECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHH
LNSMLQDVIKLGTGRRALALGRSDIAGKTGTTNESKDAWFSGYNADYVTTVWTGFDQPES
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCCCCHHH
LGRREFGGTVALPIWMNYMAAALKDKPPHVQPEPEGILSLRVDPVSGRAASPSTPGAYFE
HHHHHCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHEE
LFKAEDTPPSVNEIGNGAVPGSPLPADEQAPIDLF
EEECCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043; 7901733 [H]