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The map label for this gene is priA [H]

Identifier: 77456628

GI number: 77456628

Start: 453938

End: 456172

Strand: Reverse

Name: priA [H]

Synonym: Pfl01_0400

Alternate gene names: 77456628

Gene position: 456172-453938 (Counterclockwise)

Preceding gene: 77456632

Following gene: 77456627

Centisome position: 7.09

GC content: 66.17

Gene sequence:

>2235_bases
ATGAGAATCCCCCGCGTGCCCGACGCCATTCTGCGCCTCGCCCTGCCTTCGCCCCTGCGCCGCCTGTTCGACTATCGGGC
CCCGGCCGGTGTGCTGCGTTCGCAGTTGCAACCGGGCATGCGCCTGCGGGTGCCGTTCGGCCGACGGGAGATGATCGGGA
TTCTGGTGGAGGTCACTGACACCAGCGAAGTGCCAGTGGAGAAGCTCAAACCGGCACTGGCGCTGCTCGACGTCACCGCA
CCACTGCCGCCAGCGCTGTTCAAGCTGTGCCTGTGGACGTCTCAGTATTATCAGCACAGCCTCGGCGACACGTTGAGCTG
GGCGCTGCCGGTGCTGTTGCGCCAGGGCGAACTGGCCGAAGTTCGGCAGGAACGCTTCTGGTCCGCCGCTCCCGGCGCCA
GCCTCGATGACCCACGCATCGCCCGCGCCCCGCGCCAGCGTGAAGCCCTGGCGACTTTGGCCCAGCACCCTCACGGCGTG
GCCCATCAGTTGCTGAGCAAACTGATGCTGAGCAAGGACAGCCTTGATCTGCTGCTGGCCAAGGATCTGGTGCAGGTTGA
AGTGCGGCGTCACGCCCCCGGCGCGCGCCATGAACACTGGCTGGCCCAGCCCGAGCTGCCGCTCAACAGCGAACAGCGCG
CCGCCTATGAAGCGATTCGCGCCGGGTTCGACAGTTATCACGCGTTCCTGCTGGCCGGGGTCACTGGCAGCGGCAAGACC
GAAGTCTATTTGCAGCTGATCCGCGAAACCCTCGAGGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAGATCAACCTCGG
CCCGCAGACCCTCGCGCGGTTCGAACAACGCTTCAACGCCCGCATCGCCCTGCTGCACTCGGCGGTCAACGATCGTGATC
GTCTGGATGCCTGGCTCGCCGCCCGCGATGGCGAGGCCGACATTATTATCGGCACCCGCTCGGCGCTGTTCACCCCGATG
AAGAATCCGGGGCTGATAATCATCGACGAAGAACACGACGGCTCTTATAAACAGCAGGAAGGCCTGCGCTACCACGCGCG
GGATCTGGCGCTGGTGCGGGCGCGGCAGGAAAACATCCCGATCGTGCTCGGCTCGGCGACGCCATCCCTGGAAAGCCTGC
ACAACGCCTACACCGGCCGTTACGGACTCCTACGCCTGAACGAACGGGCCGGAGGCGCCAAGCAGCCACGTTTCCTGCGC
CTGGATGTGAAAAGTCGCCCCCTGGACAGCGGCATTTCCGGGCCGATGCAGCAAGCAATCGGCCAGACCCTCGCCGCCGG
CCAGCAGGTATTGGTGTTCCTCAACCGCCGGGGTTTTGCCCCGACGCTGCTGTGCCACGATTGTGGCTGGATGTCCGAAT
GCCAGCGCTGCGATGCGCGAATGACCGTGCACCAGCGCTACGGCGAGCTGCGCTGCCACCACTGCGGTTACGTGGAACGT
GTGCCGCGCCAGTGCCCGAAGTGCAACAAGGTCGATCTGCGCCCGGTGGGCGCCGGCACCGAGCGGGCTGAGGAACGGCT
GGCAATTCTGTTCCCGGACTATCCGGTGTTGCGCGTCGACCGTGACAGCACGTCGCGCAAGGATGCGATGAACCAGTTGT
TCGCCACGATCCAGAAAGGCCAGCCGTGCATTCTGGTCGGCACCCAGATGTTGGCCAAGGGGCACCACTTCCCTCGGGTG
ACGCTGGTGTCGATCCTCGATGCCGACGGTGGGCTGTTCTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAGTTGAT
CGTGCAGGTTGCCGGCCGTGCCGGGCGGGCGGAAGAACCGGGCAAGGTGATCATCCAGACCCACCTCGCCGACCATCCTT
TATTGGTGCAACTGACCGAGCAGGGTTACTTCGCCTTCGCCGAACAGGCGCTGAGCGAACGCCGCGCCGCCGGCCTGCCG
CCGTTCGCCCATCTCGCCCTGCTGCGCGCCGAAGCCCACAAGCCAGGGCAGGCCGAAGGTTTTCTCGATGAGGCATGCAG
CGAGGCCGAGCGCTTGCTGGCCGAACAGAACCTGAGCGGCATCGAATTGCTGGGGCCGGTGCCGGCACCGATGGAACGTC
GGGCCGGACGCTTCCGCGCGCAGCTGCTGTTGCAGGCCACGGCCCGGGCGCCGCTGCACCGATTGCTGGCGAGCTGGCTG
CTGGTGCTGGAGCAGATGCCAAGCGGTCGGGCGGTGCGCTGGTCGCTGGATGTCGATCCGGTGGATCTGTACTGA

Upstream 100 bases:

>100_bases
CGTAATTAGGCCGCGGATTCTACCAGACAATCCCGATCACGCAAGCTGTCACTGAGACCGACCGTCTGCTAGGCTCCCGC
CCTTCTCCACCGCCCCGCCT

Downstream 100 bases:

>100_bases
CCGGGCAAAACCGGCCGAATTGTCACCACACATCCACATCCTGCCTGCTAAGGTTGGCAAGCCCGTCTTCGCAACGGATA
ATGCCCAGTTTTTCCACCCG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 744; Mature: 744

Protein sequence:

>744_residues
MRIPRVPDAILRLALPSPLRRLFDYRAPAGVLRSQLQPGMRLRVPFGRREMIGILVEVTDTSEVPVEKLKPALALLDVTA
PLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAEVRQERFWSAAPGASLDDPRIARAPRQREALATLAQHPHGV
AHQLLSKLMLSKDSLDLLLAKDLVQVEVRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKT
EVYLQLIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPM
KNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLR
LDVKSRPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRYGELRCHHCGYVER
VPRQCPKCNKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRV
TLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLP
PFAHLALLRAEAHKPGQAEGFLDEACSEAERLLAEQNLSGIELLGPVPAPMERRAGRFRAQLLLQATARAPLHRLLASWL
LVLEQMPSGRAVRWSLDVDPVDLY

Sequences:

>Translated_744_residues
MRIPRVPDAILRLALPSPLRRLFDYRAPAGVLRSQLQPGMRLRVPFGRREMIGILVEVTDTSEVPVEKLKPALALLDVTA
PLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAEVRQERFWSAAPGASLDDPRIARAPRQREALATLAQHPHGV
AHQLLSKLMLSKDSLDLLLAKDLVQVEVRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKT
EVYLQLIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPM
KNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLR
LDVKSRPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRYGELRCHHCGYVER
VPRQCPKCNKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRV
TLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLP
PFAHLALLRAEAHKPGQAEGFLDEACSEAERLLAEQNLSGIELLGPVPAPMERRAGRFRAQLLLQATARAPLHRLLASWL
LVLEQMPSGRAVRWSLDVDPVDLY
>Mature_744_residues
MRIPRVPDAILRLALPSPLRRLFDYRAPAGVLRSQLQPGMRLRVPFGRREMIGILVEVTDTSEVPVEKLKPALALLDVTA
PLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAEVRQERFWSAAPGASLDDPRIARAPRQREALATLAQHPHGV
AHQLLSKLMLSKDSLDLLLAKDLVQVEVRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKT
EVYLQLIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLAARDGEADIIIGTRSALFTPM
KNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLR
LDVKSRPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRYGELRCHHCGYVER
VPRQCPKCNKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKGQPCILVGTQMLAKGHHFPRV
TLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLP
PFAHLALLRAEAHKPGQAEGFLDEACSEAERLLAEQNLSGIELLGPVPAPMERRAGRFRAQLLLQATARAPLHRLLASWL
LVLEQMPSGRAVRWSLDVDPVDLY

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=738, Percent_Identity=49.4579945799458, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=213, Percent_Identity=28.6384976525822, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 82701; Mature: 82701

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRVPDAILRLALPSPLRRLFDYRAPAGVLRSQLQPGMRLRVPFGRREMIGILVEVTD
CCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCCEEECCCCCCCEEEEEEEECC
TSEVPVEKLKPALALLDVTAPLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAE
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHH
VRQERFWSAAPGASLDDPRIARAPRQREALATLAQHPHGVAHQLLSKLMLSKDSLDLLLA
HHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
KDLVQVEVRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHEEEHEEECCCCCCH
EVYLQLIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLA
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHE
ARDGEADIIIGTRSALFTPMKNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIP
ECCCCCEEEEECHHHHCCCCCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCCCC
IVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKSRPLDSGISGPMQQAI
EEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHH
GQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRYGELRCHHCGYVER
HHHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCHHHH
VPRQCPKCNKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKG
HHHHCCCCCCCCCEECCCCCHHHHCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCC
QPCILVGTQMLAKGHHFPRVTLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEP
CCEEEECHHHHHCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCC
GKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHLALLRAEAHKPGQAEG
CEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCH
FLDEACSEAERLLAEQNLSGIELLGPVPAPMERRAGRFRAQLLLQATARAPLHRLLASWL
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLEQMPSGRAVRWSLDVDPVDLY
HHHHHCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MRIPRVPDAILRLALPSPLRRLFDYRAPAGVLRSQLQPGMRLRVPFGRREMIGILVEVTD
CCCCCCCHHHHHHHCCHHHHHHHHCCCCHHHHHHHCCCCCEEECCCCCCCEEEEEEEECC
TSEVPVEKLKPALALLDVTAPLPPALFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAE
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHH
VRQERFWSAAPGASLDDPRIARAPRQREALATLAQHPHGVAHQLLSKLMLSKDSLDLLLA
HHHHHHCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHH
KDLVQVEVRRHAPGARHEHWLAQPELPLNSEQRAAYEAIRAGFDSYHAFLLAGVTGSGKT
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHEEEHEEECCCCCCH
EVYLQLIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALLHSAVNDRDRLDAWLA
HHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHE
ARDGEADIIIGTRSALFTPMKNPGLIIIDEEHDGSYKQQEGLRYHARDLALVRARQENIP
ECCCCCEEEEECHHHHCCCCCCCCEEEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCCCC
IVLGSATPSLESLHNAYTGRYGLLRLNERAGGAKQPRFLRLDVKSRPLDSGISGPMQQAI
EEEECCCCCHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHH
GQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSECQRCDARMTVHQRYGELRCHHCGYVER
HHHHHCCCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCHHHH
VPRQCPKCNKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFATIQKG
HHHHCCCCCCCCCEECCCCCHHHHCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHCC
QPCILVGTQMLAKGHHFPRVTLVSILDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEP
CCEEEECHHHHHCCCCCCCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCC
GKVIIQTHLADHPLLVQLTEQGYFAFAEQALSERRAAGLPPFAHLALLRAEAHKPGQAEG
CEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCH
FLDEACSEAERLLAEQNLSGIELLGPVPAPMERRAGRFRAQLLLQATARAPLHRLLASWL
HHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLEQMPSGRAVRWSLDVDPVDLY
HHHHHCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]