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The map label for this gene is rsmE [H]

Identifier: 77456607

GI number: 77456607

Start: 435130

End: 435837

Strand: Reverse

Name: rsmE [H]

Synonym: Pfl01_0379

Alternate gene names: 77456607

Gene position: 435837-435130 (Counterclockwise)

Preceding gene: 77456608

Following gene: 77456606

Centisome position: 6.77

GC content: 65.96

Gene sequence:

>708_bases
ATGAACCTGCTGCTGCTCGAAGAGGCCGACTTCATTGCGGCCGACCGCGTCGTGCTGCGTGATCGGCGCCTGACCCACAT
GCAGGAAGTCCACCGTTCGGAAGTCGGCGACAGCCTGCGGGTCGGGTGCATTGGCGGGCTGATGGGCTCGGCCGAGCTTT
TGCGCCTTGACGCGGGCGAAGCCGAGCTGCGAGTCAACCTCGATCAACCGCCACCGGCCAAACTGCCGCTGACCCTGGTG
CTCGCCCTGCCCCGCCCGAAGATGCTGCGCCGGGTGTTCCAGACCGTGGCGACCATGGGCGTGTCGAAGGTGATTCTGGT
CAACAGCTACCGGGTCGAGAAAAGCTTCTGGCAGACGCCGTTCCTCGAACCGGACGCCATCCGCGAACAACTGATCCTCG
GCCTCGAACAGGCTCGGGATACGGTGCTGCCGGAGATCATCATCGAGAAGCGCTTCAAGCCGTTCGTCGAAGATCGCCTG
CCGGCCATCACCGACGGCACCCTCGGTCTGGTCGGTCATCCGGGCAACTTCCCGCCCTGCCCCCGCGCCCTGAACGAACC
GGTGACCCTGGCCATCGGCCCCGAGGGTGGCTGGATTCCCTACGAAATCGATCTGCTGGCCAAGGCCGGGCTGCAACCGG
TGCAACTGGGCGAGCGAATCCTGCGGGTCGAAACCGCCGTCACCGCGCTGCTCGCCCGGCTGTTCTGA

Upstream 100 bases:

>100_bases
TGTTCGAAGTCGGCATCCTCTTCGGCAGTCTGGTCAGCAAGCGCGAGCGCCCGGAAGAAACCGCCGACGATCACAACGAC
CAGCCGCCAGCGACTCAGCC

Downstream 100 bases:

>100_bases
GCGCTTCTGTTCTAAGCGCTTCGCCAGACAAAAGGCGTTACAGATTGCCATCATGCGGCCGATACACTCCCCATAAGTCC
AATTAGTGGTCCGAGGGGAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MNLLLLEEADFIAADRVVLRDRRLTHMQEVHRSEVGDSLRVGCIGGLMGSAELLRLDAGEAELRVNLDQPPPAKLPLTLV
LALPRPKMLRRVFQTVATMGVSKVILVNSYRVEKSFWQTPFLEPDAIREQLILGLEQARDTVLPEIIIEKRFKPFVEDRL
PAITDGTLGLVGHPGNFPPCPRALNEPVTLAIGPEGGWIPYEIDLLAKAGLQPVQLGERILRVETAVTALLARLF

Sequences:

>Translated_235_residues
MNLLLLEEADFIAADRVVLRDRRLTHMQEVHRSEVGDSLRVGCIGGLMGSAELLRLDAGEAELRVNLDQPPPAKLPLTLV
LALPRPKMLRRVFQTVATMGVSKVILVNSYRVEKSFWQTPFLEPDAIREQLILGLEQARDTVLPEIIIEKRFKPFVEDRL
PAITDGTLGLVGHPGNFPPCPRALNEPVTLAIGPEGGWIPYEIDLLAKAGLQPVQLGERILRVETAVTALLARLF
>Mature_235_residues
MNLLLLEEADFIAADRVVLRDRRLTHMQEVHRSEVGDSLRVGCIGGLMGSAELLRLDAGEAELRVNLDQPPPAKLPLTLV
LALPRPKMLRRVFQTVATMGVSKVILVNSYRVEKSFWQTPFLEPDAIREQLILGLEQARDTVLPEIIIEKRFKPFVEDRL
PAITDGTLGLVGHPGNFPPCPRALNEPVTLAIGPEGGWIPYEIDLLAKAGLQPVQLGERILRVETAVTALLARLF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26058; Mature: 26058

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLLLEEADFIAADRVVLRDRRLTHMQEVHRSEVGDSLRVGCIGGLMGSAELLRLDAGE
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCEEEEECCCC
AELRVNLDQPPPAKLPLTLVLALPRPKMLRRVFQTVATMGVSKVILVNSYRVEKSFWQTP
EEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECEEHHHHHHCCC
FLEPDAIREQLILGLEQARDTVLPEIIIEKRFKPFVEDRLPAITDGTLGLVGHPGNFPPC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCCCCC
PRALNEPVTLAIGPEGGWIPYEIDLLAKAGLQPVQLGERILRVETAVTALLARLF
CHHHCCCEEEEECCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNLLLLEEADFIAADRVVLRDRRLTHMQEVHRSEVGDSLRVGCIGGLMGSAELLRLDAGE
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCEEEEECCCC
AELRVNLDQPPPAKLPLTLVLALPRPKMLRRVFQTVATMGVSKVILVNSYRVEKSFWQTP
EEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECEEHHHHHHCCC
FLEPDAIREQLILGLEQARDTVLPEIIIEKRFKPFVEDRLPAITDGTLGLVGHPGNFPPC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCEEEEECCCCCCCC
PRALNEPVTLAIGPEGGWIPYEIDLLAKAGLQPVQLGERILRVETAVTALLARLF
CHHHCCCEEEEECCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]