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The map label for this gene is nudE [H]

Identifier: 77456512

GI number: 77456512

Start: 330594

End: 331160

Strand: Direct

Name: nudE [H]

Synonym: Pfl01_0284

Alternate gene names: 77456512

Gene position: 330594-331160 (Clockwise)

Preceding gene: 77456510

Following gene: 77456513

Centisome position: 5.13

GC content: 62.43

Gene sequence:

>567_bases
ATGCGCCAGAAACCCACCGTACTTGCCCGCGAGATCGTCGCCACCAGCCGGCTGTTCTGCGTCGAAGAGCTGAAGCTGCG
TTTCTCCAACGGCGTGGAACGTACCTACGAGCGGCTGGTCGGCAAGGGCGCCGGTTACGGCGCGGTCATGATCGTGGCGA
TGCTCGATGCCGAGCACGCGGTGTTGGTCGAGGAATACTGTGGCGGCACCGACGAATATGAACTGTCGCTGCCCAAAGGC
CTGATCGAACCCGGCGAAGATGTGCTGGCCGCCGCCGAGCGTGAGCTCAAGGAAGAAGCCGGTTACGGCGCGCGACAACT
GGAGCATCTGACCGAGCTGTCGTTGTCCCCCGGTTACATGAGCCAGAAAATTCAGGTGGTGCTGGCCACCGACCTGTACG
AGGAGCGCCTTGAAGGCGATGAGCCTGAGCCGATGCGCGTGGACAAGGTCAATCTGCGAGAGCTGTCGACGCTGGCGCAG
AATCCGAATTTCAGTGAGGGGCGCGCCCTGGCGGCGCTATACCTGACCCGCGACCTGCTGACCCAGCGCGGATTTTTCCA
GTCATGA

Upstream 100 bases:

>100_bases
TAATGAAGGCATGGCAACGTCTCGAACGGTAGAAAGATATCCGACGCAAACAATCGGAAAAGCCGCGTTATAGTAGCGCG
TTCACGCCAAGGAGCTTTGC

Downstream 100 bases:

>100_bases
GTGAGATGCCAATGAATTTTCCCCATCCGTTGATGGCGCCGGTGGTCGAGCTGGCATTGCGGGCCGGTGAGGCGATTTTG
CCGTTCTGGCGTGCCGATGT

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MRQKPTVLAREIVATSRLFCVEELKLRFSNGVERTYERLVGKGAGYGAVMIVAMLDAEHAVLVEEYCGGTDEYELSLPKG
LIEPGEDVLAAAERELKEEAGYGARQLEHLTELSLSPGYMSQKIQVVLATDLYEERLEGDEPEPMRVDKVNLRELSTLAQ
NPNFSEGRALAALYLTRDLLTQRGFFQS

Sequences:

>Translated_188_residues
MRQKPTVLAREIVATSRLFCVEELKLRFSNGVERTYERLVGKGAGYGAVMIVAMLDAEHAVLVEEYCGGTDEYELSLPKG
LIEPGEDVLAAAERELKEEAGYGARQLEHLTELSLSPGYMSQKIQVVLATDLYEERLEGDEPEPMRVDKVNLRELSTLAQ
NPNFSEGRALAALYLTRDLLTQRGFFQS
>Mature_188_residues
MRQKPTVLAREIVATSRLFCVEELKLRFSNGVERTYERLVGKGAGYGAVMIVAMLDAEHAVLVEEYCGGTDEYELSLPKG
LIEPGEDVLAAAERELKEEAGYGARQLEHLTELSLSPGYMSQKIQVVLATDLYEERLEGDEPEPMRVDKVNLRELSTLAQ
NPNFSEGRALAALYLTRDLLTQRGFFQS

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=182, Percent_Identity=50.5494505494505, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 21000; Mature: 21000

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQKPTVLAREIVATSRLFCVEELKLRFSNGVERTYERLVGKGAGYGAVMIVAMLDAEHA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
VLVEEYCGGTDEYELSLPKGLIEPGEDVLAAAERELKEEAGYGARQLEHLTELSLSPGYM
HHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
SQKIQVVLATDLYEERLEGDEPEPMRVDKVNLRELSTLAQNPNFSEGRALAALYLTRDLL
CHHHEEEHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TQRGFFQS
HHCCCCCC
>Mature Secondary Structure
MRQKPTVLAREIVATSRLFCVEELKLRFSNGVERTYERLVGKGAGYGAVMIVAMLDAEHA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
VLVEEYCGGTDEYELSLPKGLIEPGEDVLAAAERELKEEAGYGARQLEHLTELSLSPGYM
HHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
SQKIQVVLATDLYEERLEGDEPEPMRVDKVNLRELSTLAQNPNFSEGRALAALYLTRDLL
CHHHEEEHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
TQRGFFQS
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]