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The map label for this gene is pgaB [H]

Identifier: 77456406

GI number: 77456406

Start: 215343

End: 217340

Strand: Direct

Name: pgaB [H]

Synonym: Pfl01_0178

Alternate gene names: 77456406

Gene position: 215343-217340 (Clockwise)

Preceding gene: 77456405

Following gene: 77456407

Centisome position: 3.34

GC content: 63.36

Gene sequence:

>1998_bases
ATGCCTTTGATTTCGCGTTTCATCCTTCTGCTGGGAGTGCTGCTGGTCAGCGCCTGCGCCCAGCAAGCTCCGGCCTTCGT
GCCGCCGTCCGAGCGACCGCTGGCGGCCAATGAAAAGCCCTGGCCGAAAAATCACGTGCTGGGCATTGCCTACCACGACG
TCGAAGACCGTGACCCCGATCAGGCGGTGGTGGCGGTGCGCACCGAGCGCCTGCTTGAGCAACTCGCCTGGCTGCGGGAG
AACAACTACCAACCGGTGACGGTCGATCAGATCATGGCCGCGCGCAAAGGCGGGCCGGAACTGCCGCCCAAGGCGATCCT
GCTCAGTTTCGACGACGGTTACTCAAGCTTCTACACCCGAGTGTTGCCGGTGTTGCGCGCCTACAACTGGCATGCGCTGC
TGGCGCCGGTCGGCACCTGGATCGACACGCCGCTGAACCAACCGGTGGATTTCGCCGGTACACCGCGTCCGCGCTCGGAC
TTCCTGACCTGGGCTCAGGTGCGGGAAATCTCGCAATCGGGCCTGGTGGAAATCGCCGCCCACACCGACGCCAGCCACAA
AGGCATCCTCGCCAACCCGCAAGGCAACCTGCAGCCGGCGGCCGCGACCCGACGCTACGATCCGGTGAGCAAACGCTACG
AATCCGAAGCCGATTTCCAGGCGCGGATCCGCGCCGACGTGCAGGCGATCTCGGAGAAGATCCGCAAGAACACCGGTAAA
AAACCGCGCATTTGGGTCTGGCCTTACGGGACGGCGGACGGAACCTCGCTGACCGTGGTCAGCGAAGAGGGCTATGAAAT
GGCCCTGACCCTCGATGACGGCCTCGATGCCCTCGACAACCTGATGAGCGGGCCGCGTTTCCTGGTGGCTTCCGACCCTG
ACGGCGAGCACTTCGCCAACAGCGTGGTCGGCGTGCAAGCGGATTTCGTGATGCGCGTGGTCCACGTCGATCTGGACAAC
GTCTACGACCCGGACCCGGCGCAGCAGGAAATCAACCTCGGCAAATTGATCCAGCGCATGGCCGACATGGGCGCCAACAC
CGTGTTCCTGCAAGCCTTCGCCGATCCGAAGGGCGATGGCCTGGTGCATTCGCTGTACTTCCCCAACCGTCATCTGCCGA
TGCGCGCCGACCTGTTCGACCGCGTCGCCTGGCAATTGCGCACCCGGGCTCACGTGAAGGTTTTCGCGTGGATGCCGGTG
CTGAGTTTCGAGCTGGATTCGAAGTTGCCACGGGTGACGCGCTGGGATCCGAAGACCGGCACGACCGCAGTCGACCCGGA
TCAATACAAACGGCTGTCGCCGTTCGATCCCGAAGTGCGGCGGATCATCGGCGAGATCTACGAAGACGTAGCACGCCTGA
CTTCGGTCGACGGCATTCTCTATCACGACGACGCAGTGCTTTCGGACTTCGAAGACGCCGGCCCGCAGGCGCTGAAGGCT
TACACCGCCCAAGGCTTGCCGGGCTCGATCGCCGCTCTGCGTGACGATCCGGCAGCCATGCAGCGCTGGACGCGCTTCAA
GAGCCGCTACCTGATCGACTTCACCAATGAGCTGACCGCCAAGGTGCGCGCGATTCGTGGCCCGCAAGTGCTCACCGCAC
GCAACATCTTCGCCGAGCCGGTGCTCAATCCGCACAGCGAAGCGTGGTTCGCGCAGAACCTCGACGACTTCCTCGGGACT
TACGACTGGACGGCGCCGATGGCCATGCCGCTCATGGAAAAACAGACGTATGCGCAGTCCGGCCCGTGGCTCGAAGAACT
GGTGGCGAAGATCAAACAACGCCCCGGCGCGCTGGATCGCACGGTGTTCGAACTGCAGGCCCGGGACTGGACGAAAAAGG
ACCAGGCCGACATCGATGGCGCACAACTGGCCGACTGGATGAGCCGCCTCAAACGTCAGGGCGCCACCAGTTTCGGCTAC
TACCCGGACAACTTTCTCGAGAACCTGCCGGACCTGAAAACCGTGCGGCCTGCGCTCTCCAACAAATGGAACCCATGA

Upstream 100 bases:

>100_bases
CGGTTTGTTCAGCGTGATCAACCGGCCCTACGACGGTGACCGGGAAACCGATCTGCGTCTGCTCGTCGACCTCACTTTCC
GCTTCTAGAAGAGTTTGAAG

Downstream 100 bases:

>100_bases
CATGCTGGATAGACTGCTGGCCCTGCTTGTTCTGGCGATCGTCCTCGGCGTCCCGCTGGGGCTGATCTTCCTGGTCACCG
GGCAATTCCTGATGGACTTC

Product: outer membrane N-deacetylase

Products: NA

Alternate protein names: PGA N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MPLISRFILLLGVLLVSACAQQAPAFVPPSERPLAANEKPWPKNHVLGIAYHDVEDRDPDQAVVAVRTERLLEQLAWLRE
NNYQPVTVDQIMAARKGGPELPPKAILLSFDDGYSSFYTRVLPVLRAYNWHALLAPVGTWIDTPLNQPVDFAGTPRPRSD
FLTWAQVREISQSGLVEIAAHTDASHKGILANPQGNLQPAAATRRYDPVSKRYESEADFQARIRADVQAISEKIRKNTGK
KPRIWVWPYGTADGTSLTVVSEEGYEMALTLDDGLDALDNLMSGPRFLVASDPDGEHFANSVVGVQADFVMRVVHVDLDN
VYDPDPAQQEINLGKLIQRMADMGANTVFLQAFADPKGDGLVHSLYFPNRHLPMRADLFDRVAWQLRTRAHVKVFAWMPV
LSFELDSKLPRVTRWDPKTGTTAVDPDQYKRLSPFDPEVRRIIGEIYEDVARLTSVDGILYHDDAVLSDFEDAGPQALKA
YTAQGLPGSIAALRDDPAAMQRWTRFKSRYLIDFTNELTAKVRAIRGPQVLTARNIFAEPVLNPHSEAWFAQNLDDFLGT
YDWTAPMAMPLMEKQTYAQSGPWLEELVAKIKQRPGALDRTVFELQARDWTKKDQADIDGAQLADWMSRLKRQGATSFGY
YPDNFLENLPDLKTVRPALSNKWNP

Sequences:

>Translated_665_residues
MPLISRFILLLGVLLVSACAQQAPAFVPPSERPLAANEKPWPKNHVLGIAYHDVEDRDPDQAVVAVRTERLLEQLAWLRE
NNYQPVTVDQIMAARKGGPELPPKAILLSFDDGYSSFYTRVLPVLRAYNWHALLAPVGTWIDTPLNQPVDFAGTPRPRSD
FLTWAQVREISQSGLVEIAAHTDASHKGILANPQGNLQPAAATRRYDPVSKRYESEADFQARIRADVQAISEKIRKNTGK
KPRIWVWPYGTADGTSLTVVSEEGYEMALTLDDGLDALDNLMSGPRFLVASDPDGEHFANSVVGVQADFVMRVVHVDLDN
VYDPDPAQQEINLGKLIQRMADMGANTVFLQAFADPKGDGLVHSLYFPNRHLPMRADLFDRVAWQLRTRAHVKVFAWMPV
LSFELDSKLPRVTRWDPKTGTTAVDPDQYKRLSPFDPEVRRIIGEIYEDVARLTSVDGILYHDDAVLSDFEDAGPQALKA
YTAQGLPGSIAALRDDPAAMQRWTRFKSRYLIDFTNELTAKVRAIRGPQVLTARNIFAEPVLNPHSEAWFAQNLDDFLGT
YDWTAPMAMPLMEKQTYAQSGPWLEELVAKIKQRPGALDRTVFELQARDWTKKDQADIDGAQLADWMSRLKRQGATSFGY
YPDNFLENLPDLKTVRPALSNKWNP
>Mature_664_residues
PLISRFILLLGVLLVSACAQQAPAFVPPSERPLAANEKPWPKNHVLGIAYHDVEDRDPDQAVVAVRTERLLEQLAWLREN
NYQPVTVDQIMAARKGGPELPPKAILLSFDDGYSSFYTRVLPVLRAYNWHALLAPVGTWIDTPLNQPVDFAGTPRPRSDF
LTWAQVREISQSGLVEIAAHTDASHKGILANPQGNLQPAAATRRYDPVSKRYESEADFQARIRADVQAISEKIRKNTGKK
PRIWVWPYGTADGTSLTVVSEEGYEMALTLDDGLDALDNLMSGPRFLVASDPDGEHFANSVVGVQADFVMRVVHVDLDNV
YDPDPAQQEINLGKLIQRMADMGANTVFLQAFADPKGDGLVHSLYFPNRHLPMRADLFDRVAWQLRTRAHVKVFAWMPVL
SFELDSKLPRVTRWDPKTGTTAVDPDQYKRLSPFDPEVRRIIGEIYEDVARLTSVDGILYHDDAVLSDFEDAGPQALKAY
TAQGLPGSIAALRDDPAAMQRWTRFKSRYLIDFTNELTAKVRAIRGPQVLTARNIFAEPVLNPHSEAWFAQNLDDFLGTY
DWTAPMAMPLMEKQTYAQSGPWLEELVAKIKQRPGALDRTVFELQARDWTKKDQADIDGAQLADWMSRLKRQGATSFGYY
PDNFLENLPDLKTVRPALSNKWNP

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PGA), a biofilm adhesin polysaccharide. N- deacetylation promotes PGA export through the PgaA porin [H]

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1787260, Length=661, Percent_Identity=48.8653555219365, Blast_Score=669, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 74723; Mature: 74592

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLISRFILLLGVLLVSACAQQAPAFVPPSERPLAANEKPWPKNHVLGIAYHDVEDRDPD
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
QAVVAVRTERLLEQLAWLRENNYQPVTVDQIMAARKGGPELPPKAILLSFDDGYSSFYTR
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHH
VLPVLRAYNWHALLAPVGTWIDTPLNQPVDFAGTPRPRSDFLTWAQVREISQSGLVEIAA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
HTDASHKGILANPQGNLQPAAATRRYDPVSKRYESEADFQARIRADVQAISEKIRKNTGK
CCCCCCCCEEECCCCCCCCHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
KPRIWVWPYGTADGTSLTVVSEEGYEMALTLDDGLDALDNLMSGPRFLVASDPDGEHFAN
CCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHCCCEEEEECCCCCHHHHH
SVVGVQADFVMRVVHVDLDNVYDPDPAQQEINLGKLIQRMADMGANTVFLQAFADPKGDG
HHHCCHHHHHHHHHHCCHHCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCCCC
LVHSLYFPNRHLPMRADLFDRVAWQLRTRAHVKVFAWMPVLSFELDSKLPRVTRWDPKTG
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCC
TTAVDPDQYKRLSPFDPEVRRIIGEIYEDVARLTSVDGILYHDDAVLSDFEDAGPQALKA
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCHHHHHH
YTAQGLPGSIAALRDDPAAMQRWTRFKSRYLIDFTNELTAKVRAIRGPQVLTARNIFAEP
HHCCCCCCCHHHHCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHCCCCEEEHHHHHHCC
VLNPHSEAWFAQNLDDFLGTYDWTAPMAMPLMEKQTYAQSGPWLEELVAKIKQRPGALDR
CCCCCCHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
TVFELQARDWTKKDQADIDGAQLADWMSRLKRQGATSFGYYPDNFLENLPDLKTVRPALS
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHC
NKWNP
CCCCC
>Mature Secondary Structure 
PLISRFILLLGVLLVSACAQQAPAFVPPSERPLAANEKPWPKNHVLGIAYHDVEDRDPD
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCC
QAVVAVRTERLLEQLAWLRENNYQPVTVDQIMAARKGGPELPPKAILLSFDDGYSSFYTR
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHH
VLPVLRAYNWHALLAPVGTWIDTPLNQPVDFAGTPRPRSDFLTWAQVREISQSGLVEIAA
HHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
HTDASHKGILANPQGNLQPAAATRRYDPVSKRYESEADFQARIRADVQAISEKIRKNTGK
CCCCCCCCEEECCCCCCCCHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCC
KPRIWVWPYGTADGTSLTVVSEEGYEMALTLDDGLDALDNLMSGPRFLVASDPDGEHFAN
CCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHCCCEEEEECCCCCHHHHH
SVVGVQADFVMRVVHVDLDNVYDPDPAQQEINLGKLIQRMADMGANTVFLQAFADPKGDG
HHHCCHHHHHHHHHHCCHHCCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEEECCCCCCC
LVHSLYFPNRHLPMRADLFDRVAWQLRTRAHVKVFAWMPVLSFELDSKLPRVTRWDPKTG
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCC
TTAVDPDQYKRLSPFDPEVRRIIGEIYEDVARLTSVDGILYHDDAVLSDFEDAGPQALKA
CCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCHHHHHH
YTAQGLPGSIAALRDDPAAMQRWTRFKSRYLIDFTNELTAKVRAIRGPQVLTARNIFAEP
HHCCCCCCCHHHHCCCHHHHHHHHHHHHCEEEEEHHHHHHHHHHHCCCCEEEHHHHHHCC
VLNPHSEAWFAQNLDDFLGTYDWTAPMAMPLMEKQTYAQSGPWLEELVAKIKQRPGALDR
CCCCCCHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHH
TVFELQARDWTKKDQADIDGAQLADWMSRLKRQGATSFGYYPDNFLENLPDLKTVRPALS
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCHHHHHHHHC
NKWNP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]