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The map label for this gene is pgaC [H]

Identifier: 77456407

GI number: 77456407

Start: 217342

End: 218697

Strand: Direct

Name: pgaC [H]

Synonym: Pfl01_0179

Alternate gene names: 77456407

Gene position: 217342-218697 (Clockwise)

Preceding gene: 77456406

Following gene: 77456408

Centisome position: 3.38

GC content: 62.24

Gene sequence:

>1356_bases
ATGCTGGATAGACTGCTGGCCCTGCTTGTTCTGGCGATCGTCCTCGGCGTCCCGCTGGGGCTGATCTTCCTGGTCACCGG
GCAATTCCTGATGGACTTCGTGTTCTTCTACCCGCTGTTCATGTCCGGGTTGTGGATTTCCGGTGGCCTGTATTTCTGGC
TGCACTGGGAACGGCACTGGCCGTGGAAGGACGACACCCTGCCGCCACCGCTGGAAGGCGAACCGCTGATTTCGATCCTG
ATCCCTTGCTACAACGAGGGCGATAACGCCGCCGAAACTATCCACGCGGCGCTGGCGCAGCATTACCCGAACATCGAAGT
CATCGCGATCAACGACGGCTCCAGGGACAACACCGCCGAAGTGCTGGATGCGCTGGCCAAGGAAGACCCACGGCTGCGGG
TGCTGCATCTGGCGGAAAACCAGGGCAAGGCCGTGGCCCTGCGCATGGGCGCCATCGCGGCGCGCAGCGAATATCTGGTG
TGCATCGACGGTGATGCGCTGCTGGCGCCGAACACCGCCGCTTATCTGGTAGCGCCGATGCTCGACAACGCACGACTGGG
CGCGGTGACCGGCAACCCACGGATCCGCACGCGTTCGACTCTGGTCGGGCGGGTTCAGGTCGGCGAGTTCTCGTCGATCA
TCGGGCTGATCAAACGCACACAGCGGGTGTTCGGGCGGATCTTCACTGTGTCGGGTGTGATCGTTGCGTTCCGCCGCACG
GCGCTGAACCGGGTCGGCTACTGGAGCCCGGACATGATCACCGAAGACATCGACATCAGCTGGAAGCTGCAACTGGACCA
CTGGAGCATCTTTTACGAGCCCCGTGCCCTGTGCTGGATCCTGATGCCGGAAACCCTGCGCGGTCTGTGGAAGCAGCGCC
TGCGCTGGGCCCAGGGTGGTGCCGAAGTGCTGTTCAAGAACATTCGCGGGATCTGGCAGTACCGCCATCGTTACCTGTGG
CCGCTGCTGTTCGAATACTGCCTGTCGACCGGCTGGGCGTTCACGTTCCTGTTGTCGGTGATCTTCTGGGCCGTCGGCAA
GTTCGTGGTCATGCCTGAGGCGATTGCCGTCGATCACCTGATGCCGCCAGCCTTCACCGGTTTGCTGCTGGCGTTTGTCT
GCCTGGTGCAGTTCGCGGTCAGCATCGTCATCGACCGGCGTTATGAGCCGGGCCTGGGCAGGACCATGTTCTGGGTGGTG
TGGTACCCGATCGCGTTCTGGCTCGTCAGCCTGCTGACCACGCTGGTCAGTTTCCCCAAAGTCTTGTTCGGCCAACACCA
GAAACGTGCGCGCTGGGTCAGTCCCGACCGGGGTATCAAGCCGCTCGGGGACGATGAAGAGGAGGTTATCAAATGA

Upstream 100 bases:

>100_bases
GGCGCCACCAGTTTCGGCTACTACCCGGACAACTTTCTCGAGAACCTGCCGGACCTGAAAACCGTGCGGCCTGCGCTCTC
CAACAAATGGAACCCATGAC

Downstream 100 bases:

>100_bases
AAATCATCCGGACCCGGCAACGACCGTTTCTGGTGCTGCTCGATGCGTTCTTCACCGTGCTGGCCTGGATCGGCCTGCTG
TATCTGCTGGTACGCGGTCT

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PGA synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm PGA synthesis protein PgaC; N-acetylglucosaminyltransferase PgaC [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLDRLLALLVLAIVLGVPLGLIFLVTGQFLMDFVFFYPLFMSGLWISGGLYFWLHWERHWPWKDDTLPPPLEGEPLISIL
IPCYNEGDNAAETIHAALAQHYPNIEVIAINDGSRDNTAEVLDALAKEDPRLRVLHLAENQGKAVALRMGAIAARSEYLV
CIDGDALLAPNTAAYLVAPMLDNARLGAVTGNPRIRTRSTLVGRVQVGEFSSIIGLIKRTQRVFGRIFTVSGVIVAFRRT
ALNRVGYWSPDMITEDIDISWKLQLDHWSIFYEPRALCWILMPETLRGLWKQRLRWAQGGAEVLFKNIRGIWQYRHRYLW
PLLFEYCLSTGWAFTFLLSVIFWAVGKFVVMPEAIAVDHLMPPAFTGLLLAFVCLVQFAVSIVIDRRYEPGLGRTMFWVV
WYPIAFWLVSLLTTLVSFPKVLFGQHQKRARWVSPDRGIKPLGDDEEEVIK

Sequences:

>Translated_451_residues
MLDRLLALLVLAIVLGVPLGLIFLVTGQFLMDFVFFYPLFMSGLWISGGLYFWLHWERHWPWKDDTLPPPLEGEPLISIL
IPCYNEGDNAAETIHAALAQHYPNIEVIAINDGSRDNTAEVLDALAKEDPRLRVLHLAENQGKAVALRMGAIAARSEYLV
CIDGDALLAPNTAAYLVAPMLDNARLGAVTGNPRIRTRSTLVGRVQVGEFSSIIGLIKRTQRVFGRIFTVSGVIVAFRRT
ALNRVGYWSPDMITEDIDISWKLQLDHWSIFYEPRALCWILMPETLRGLWKQRLRWAQGGAEVLFKNIRGIWQYRHRYLW
PLLFEYCLSTGWAFTFLLSVIFWAVGKFVVMPEAIAVDHLMPPAFTGLLLAFVCLVQFAVSIVIDRRYEPGLGRTMFWVV
WYPIAFWLVSLLTTLVSFPKVLFGQHQKRARWVSPDRGIKPLGDDEEEVIK
>Mature_451_residues
MLDRLLALLVLAIVLGVPLGLIFLVTGQFLMDFVFFYPLFMSGLWISGGLYFWLHWERHWPWKDDTLPPPLEGEPLISIL
IPCYNEGDNAAETIHAALAQHYPNIEVIAINDGSRDNTAEVLDALAKEDPRLRVLHLAENQGKAVALRMGAIAARSEYLV
CIDGDALLAPNTAAYLVAPMLDNARLGAVTGNPRIRTRSTLVGRVQVGEFSSIIGLIKRTQRVFGRIFTVSGVIVAFRRT
ALNRVGYWSPDMITEDIDISWKLQLDHWSIFYEPRALCWILMPETLRGLWKQRLRWAQGGAEVLFKNIRGIWQYRHRYLW
PLLFEYCLSTGWAFTFLLSVIFWAVGKFVVMPEAIAVDHLMPPAFTGLLLAFVCLVQFAVSIVIDRRYEPGLGRTMFWVV
WYPIAFWLVSLLTTLVSFPKVLFGQHQKRARWVSPDRGIKPLGDDEEEVIK

Specific function: Probable N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N- acetylglucosamine to produce the linear homopolymer poly-beta-1,6- N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide [H]

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=439, Percent_Identity=61.7312072892939, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1790044, Length=113, Percent_Identity=31.858407079646, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786560, Length=215, Percent_Identity=28.3720930232558, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 51355; Mature: 51355

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLLALLVLAIVLGVPLGLIFLVTGQFLMDFVFFYPLFMSGLWISGGLYFWLHWERHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEEEEECC
PWKDDTLPPPLEGEPLISILIPCYNEGDNAAETIHAALAQHYPNIEVIAINDGSRDNTAE
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHH
VLDALAKEDPRLRVLHLAENQGKAVALRMGAIAARSEYLVCIDGDALLAPNTAAYLVAPM
HHHHHHCCCCCEEEEEEECCCCCEEEEEEHHHCCCCCEEEEECCCEEECCCCHHHEEEEH
LDNARLGAVTGNPRIRTRSTLVGRVQVGEFSSIIGLIKRTQRVFGRIFTVSGVIVAFRRT
HCCCEEEEECCCCCEEEHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNRVGYWSPDMITEDIDISWKLQLDHWSIFYEPRALCWILMPETLRGLWKQRLRWAQGG
HHHHCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECHHHHHHHHHHHHHHHCCH
AEVLFKNIRGIWQYRHRYLWPLLFEYCLSTGWAFTFLLSVIFWAVGKFVVMPEAIAVDHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
MPPAFTGLLLAFVCLVQFAVSIVIDRRYEPGLGRTMFWVVWYPIAFWLVSLLTTLVSFPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFGQHQKRARWVSPDRGIKPLGDDEEEVIK
HHHCCCHHHCCCCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MLDRLLALLVLAIVLGVPLGLIFLVTGQFLMDFVFFYPLFMSGLWISGGLYFWLHWERHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEEEEEECC
PWKDDTLPPPLEGEPLISILIPCYNEGDNAAETIHAALAQHYPNIEVIAINDGSRDNTAE
CCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHH
VLDALAKEDPRLRVLHLAENQGKAVALRMGAIAARSEYLVCIDGDALLAPNTAAYLVAPM
HHHHHHCCCCCEEEEEEECCCCCEEEEEEHHHCCCCCEEEEECCCEEECCCCHHHEEEEH
LDNARLGAVTGNPRIRTRSTLVGRVQVGEFSSIIGLIKRTQRVFGRIFTVSGVIVAFRRT
HCCCEEEEECCCCCEEEHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALNRVGYWSPDMITEDIDISWKLQLDHWSIFYEPRALCWILMPETLRGLWKQRLRWAQGG
HHHHCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCEEEEECHHHHHHHHHHHHHHHCCH
AEVLFKNIRGIWQYRHRYLWPLLFEYCLSTGWAFTFLLSVIFWAVGKFVVMPEAIAVDHL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHC
MPPAFTGLLLAFVCLVQFAVSIVIDRRYEPGLGRTMFWVVWYPIAFWLVSLLTTLVSFPK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLFGQHQKRARWVSPDRGIKPLGDDEEEVIK
HHHCCCHHHCCCCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]