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The map label for this gene is pgaA [H]

Identifier: 77456405

GI number: 77456405

Start: 212850

End: 215330

Strand: Direct

Name: pgaA [H]

Synonym: Pfl01_0177

Alternate gene names: 77456405

Gene position: 212850-215330 (Clockwise)

Preceding gene: 77456404

Following gene: 77456406

Centisome position: 3.31

GC content: 65.34

Gene sequence:

>2481_bases
ATGCCGCGTATTGATGGTCCTGTATTACATCGTGGTTTACGCCCTTTGTTTCGAGTCGCGCTGTGCAGCCAGTTGCTCTG
GCCGGCGTTCGCGTTCGCCGACACGGCCTACGATCAAATGGTGCTTGACGCCCGGGCCGGGCATTACACGCCCGCGCTGA
GCGTGTTGCGTCAGGTGCCGCCGGCCCAGGCCAGCACCGGCCAAGTCAGCGATCACCTGCAAATCGCCAGTTGGGCCGGG
CTCGACGCCGAAGTGGTGCAGGTCTACGAGACTCAGGGCCGTGAGCGTGCACTGCCGGTTCAGGCGCTGACCGCCACCGC
CCGGGCCTACCGCAACCTGAAACGCTGGGATCAGGCGATCCAGGTCTACAACAAGGCCCTGACGCTGGAGCCGGACAACG
CTGACCTGCAACTCGGTCTGGCGCTGACCCAGGCCGACTCCGGCAAGCCTGACGAAGGCGTGATCCGCGCCCGTTCGCTG
GTCGCCGCCAAACCCGATGATCCTTCCCGCCGACTGGCGCTGGGCTACGCCCTGAATCGCGCCGGCAAGCCGTACGACGC
GCTGTTCGAATACGACCAGGCCTTCATCCGCGCCGGCAACAAACCCGAGGTCGCCCGCGAATACGTGGTCGCCCTGCAAA
AGGCGCGTCTGCCGGAGCCGGCGCTGCGTCTTGCCAGACAGCGTCCGGGGTTGATCGATCCGGTCACCTTGCGGCGTCTG
GCAGGCGATCTGGCCGCTGAACGCGTGCGCCTCGCCGAACTGGCCACCCGCAGCGAAAAAGAACGCTACGTGATCGCCGA
CCGCGCCCTGGCCGACTACGACCAGCTCCTCGCCACCTGGACTCCGGACGCCACCGCCCACGACGACGTGACCCGCTGGC
GCATCGACCGCTTGGGCGCACTCAAGGCCCGAGCGCGCACCGCCGAAGTCATCAGCGAATATCAGAAGCTGCAAGCCGAA
GGCGTGAAGATCCCGACCTACGCCCTGCGCTGGGTGGCGGCGTCCTATCTGGATCAGCGCCAGCCGGAAATCGCCACCGA
TCTCTACCGTCAGGTGCTGGCAGCGCCAGATGCCGATGTCGGTGACCGACTCGAAGACACCACGGCGCTGTATTACTCGC
TGCTGGAAAGCGACCGCGCTGAAGAGGCGCGCAAAGTCGCTGAAGATCAGGCCAGTGCGCAGAAGCCGCGCGTCGAACTC
AAGGGCCTGCCGATCGGCAACCCCAACGACGAATGGATGGACGCCCAGCAACTGGCGGCACAGGCCGGCACCTATGGCTC
CGACCTGCCTCACGGCGAGGAGCGCTTGCAGACCTTGGTGGATCAGGCGCCGGGCAACATCGGCCTGCGCCTGGCCCAGG
CCGATCTGTACCTGGCCCGAAACTGGCCGCGCCGCGCTGAAAACCAGCTCAAGGAAACCGAGAGCATCGCGCCGCGCGAC
ACGGGTTTGGAAGTCGCGCAGGCTCGCACCGCGATGGACTTGCAGGAATGGCGGCAAATGGATGCGCTGACCGATGACGT
GGTGGCGCGCTTCCTCGACAACCGTCAGGTCCAGCGTCTGGCCCGCGAGCGTGAAGTGCACGACATGTCCGAGCTGCGTG
TCGAAGCCTATGGCGGCAAGGCTAACGGAGGCAGCGGTGGCGATGCCGGCGCGGTCTCCGGTAGCCGGGATTTCGGCATC
CAGACCACCCTTTACAGCCCGCCGATCGATGAGGACTGGCGAGTGTTCGCCGGGGCCGGATACGCCACCGGCGATTTTGC
CGAAGGCACCGGCCACCACCGCTTCCAGCGTGTCGGCCTGGAGCGTCGCACCCGCGACATGACCCTCGAAGCCGAAGTGT
CGAATCATTCCTACGGTTTCGGCGACAAACAGGGCGCGCGTCTGGCGATTGCCCGCGATATCAACGACCACTGGCAATAC
GGCGGCAGCCTTGAATACCTCTCGGCCGAAACTCCGCTGCGGGCATTGAACAGCGACATCAAGGCCAACGGCGGCAGCGG
TTTCATCCGTTGGCGCGCCAACGAGAGCCGCGAGTGGCGGCTGGCGGTCAGCCCGTCGCACTTCAGCGACGGCAACAACC
GCGTCGAAGCCTTGCTGACCGGAAAAGAGGGCGTCTACAGCGCGCCGAACCTGCAAGTCGACGCGGGCCTGGAAGTGGGC
ACCAGCCACAACTCCAAGTCCGATGACGTGCCGTACTTCAACCCGAAGTCGGACTTCAGTGTCATGCCGCTGCTCAACGT
CAATCACGTGCTGTACCACCGCTACGAAACCGCCTGGAGCCAGCAGTTCCAGGCCGGTGCGGGGACTTACAGCCAGCGTG
ATCACGGCACCGGCGGCATGGCATTGCTCGGTTACGGCCAGCGCTACGCCTGGAACGACGTGTTCGAGGTGGGCGGTTTG
TTCAGCGTGATCAACCGGCCCTACGACGGTGACCGGGAAACCGATCTGCGTCTGCTCGTCGACCTCACTTTCCGCTTCTA
G

Upstream 100 bases:

>100_bases
CTGCTTGGATAAGCTTGGCCAGGCAAATCGACCTGGCCTCCGTTACGAATTGGGCAAGGGGGGCGTTGCTACCCGGCTGG
CCATGTAAGGATGCTGACGA

Downstream 100 bases:

>100_bases
AAGAGTTTGAAGATGCCTTTGATTTCGCGTTTCATCCTTCTGCTGGGAGTGCTGCTGGTCAGCGCCTGCGCCCAGCAAGC
TCCGGCCTTCGTGCCGCCGT

Product: outer membrane protein PgaA

Products: NA

Alternate protein names: PGA export protein; Poly-beta-1,6-GlcNAc export protein [H]

Number of amino acids: Translated: 826; Mature: 825

Protein sequence:

>826_residues
MPRIDGPVLHRGLRPLFRVALCSQLLWPAFAFADTAYDQMVLDARAGHYTPALSVLRQVPPAQASTGQVSDHLQIASWAG
LDAEVVQVYETQGRERALPVQALTATARAYRNLKRWDQAIQVYNKALTLEPDNADLQLGLALTQADSGKPDEGVIRARSL
VAAKPDDPSRRLALGYALNRAGKPYDALFEYDQAFIRAGNKPEVAREYVVALQKARLPEPALRLARQRPGLIDPVTLRRL
AGDLAAERVRLAELATRSEKERYVIADRALADYDQLLATWTPDATAHDDVTRWRIDRLGALKARARTAEVISEYQKLQAE
GVKIPTYALRWVAASYLDQRQPEIATDLYRQVLAAPDADVGDRLEDTTALYYSLLESDRAEEARKVAEDQASAQKPRVEL
KGLPIGNPNDEWMDAQQLAAQAGTYGSDLPHGEERLQTLVDQAPGNIGLRLAQADLYLARNWPRRAENQLKETESIAPRD
TGLEVAQARTAMDLQEWRQMDALTDDVVARFLDNRQVQRLAREREVHDMSELRVEAYGGKANGGSGGDAGAVSGSRDFGI
QTTLYSPPIDEDWRVFAGAGYATGDFAEGTGHHRFQRVGLERRTRDMTLEAEVSNHSYGFGDKQGARLAIARDINDHWQY
GGSLEYLSAETPLRALNSDIKANGGSGFIRWRANESREWRLAVSPSHFSDGNNRVEALLTGKEGVYSAPNLQVDAGLEVG
TSHNSKSDDVPYFNPKSDFSVMPLLNVNHVLYHRYETAWSQQFQAGAGTYSQRDHGTGGMALLGYGQRYAWNDVFEVGGL
FSVINRPYDGDRETDLRLLVDLTFRF

Sequences:

>Translated_826_residues
MPRIDGPVLHRGLRPLFRVALCSQLLWPAFAFADTAYDQMVLDARAGHYTPALSVLRQVPPAQASTGQVSDHLQIASWAG
LDAEVVQVYETQGRERALPVQALTATARAYRNLKRWDQAIQVYNKALTLEPDNADLQLGLALTQADSGKPDEGVIRARSL
VAAKPDDPSRRLALGYALNRAGKPYDALFEYDQAFIRAGNKPEVAREYVVALQKARLPEPALRLARQRPGLIDPVTLRRL
AGDLAAERVRLAELATRSEKERYVIADRALADYDQLLATWTPDATAHDDVTRWRIDRLGALKARARTAEVISEYQKLQAE
GVKIPTYALRWVAASYLDQRQPEIATDLYRQVLAAPDADVGDRLEDTTALYYSLLESDRAEEARKVAEDQASAQKPRVEL
KGLPIGNPNDEWMDAQQLAAQAGTYGSDLPHGEERLQTLVDQAPGNIGLRLAQADLYLARNWPRRAENQLKETESIAPRD
TGLEVAQARTAMDLQEWRQMDALTDDVVARFLDNRQVQRLAREREVHDMSELRVEAYGGKANGGSGGDAGAVSGSRDFGI
QTTLYSPPIDEDWRVFAGAGYATGDFAEGTGHHRFQRVGLERRTRDMTLEAEVSNHSYGFGDKQGARLAIARDINDHWQY
GGSLEYLSAETPLRALNSDIKANGGSGFIRWRANESREWRLAVSPSHFSDGNNRVEALLTGKEGVYSAPNLQVDAGLEVG
TSHNSKSDDVPYFNPKSDFSVMPLLNVNHVLYHRYETAWSQQFQAGAGTYSQRDHGTGGMALLGYGQRYAWNDVFEVGGL
FSVINRPYDGDRETDLRLLVDLTFRF
>Mature_825_residues
PRIDGPVLHRGLRPLFRVALCSQLLWPAFAFADTAYDQMVLDARAGHYTPALSVLRQVPPAQASTGQVSDHLQIASWAGL
DAEVVQVYETQGRERALPVQALTATARAYRNLKRWDQAIQVYNKALTLEPDNADLQLGLALTQADSGKPDEGVIRARSLV
AAKPDDPSRRLALGYALNRAGKPYDALFEYDQAFIRAGNKPEVAREYVVALQKARLPEPALRLARQRPGLIDPVTLRRLA
GDLAAERVRLAELATRSEKERYVIADRALADYDQLLATWTPDATAHDDVTRWRIDRLGALKARARTAEVISEYQKLQAEG
VKIPTYALRWVAASYLDQRQPEIATDLYRQVLAAPDADVGDRLEDTTALYYSLLESDRAEEARKVAEDQASAQKPRVELK
GLPIGNPNDEWMDAQQLAAQAGTYGSDLPHGEERLQTLVDQAPGNIGLRLAQADLYLARNWPRRAENQLKETESIAPRDT
GLEVAQARTAMDLQEWRQMDALTDDVVARFLDNRQVQRLAREREVHDMSELRVEAYGGKANGGSGGDAGAVSGSRDFGIQ
TTLYSPPIDEDWRVFAGAGYATGDFAEGTGHHRFQRVGLERRTRDMTLEAEVSNHSYGFGDKQGARLAIARDINDHWQYG
GSLEYLSAETPLRALNSDIKANGGSGFIRWRANESREWRLAVSPSHFSDGNNRVEALLTGKEGVYSAPNLQVDAGLEVGT
SHNSKSDDVPYFNPKSDFSVMPLLNVNHVLYHRYETAWSQQFQAGAGTYSQRDHGTGGMALLGYGQRYAWNDVFEVGGLF
SVINRPYDGDRETDLRLLVDLTFRF

Specific function: Exports the biofilm adhesin polysaccharide poly-beta- 1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N- deacetylation of PGA by PgaB is needed for PGA export through the PgaA pori

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 TPR repeats [H]

Homologues:

Organism=Escherichia coli, GI1787261, Length=803, Percent_Identity=39.8505603985056, Blast_Score=533, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013026
- InterPro:   IPR011990
- InterPro:   IPR019734 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 91816; Mature: 91685

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRIDGPVLHRGLRPLFRVALCSQLLWPAFAFADTAYDQMVLDARAGHYTPALSVLRQVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
PAQASTGQVSDHLQIASWAGLDAEVVQVYETQGRERALPVQALTATARAYRNLKRWDQAI
CCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QVYNKALTLEPDNADLQLGLALTQADSGKPDEGVIRARSLVAAKPDDPSRRLALGYALNR
HHHHHHEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHEECCCCCCHHHEEHHHHHHC
AGKPYDALFEYDQAFIRAGNKPEVAREYVVALQKARLPEPALRLARQRPGLIDPVTLRRL
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
AGDLAAERVRLAELATRSEKERYVIADRALADYDQLLATWTPDATAHDDVTRWRIDRLGA
HHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LKARARTAEVISEYQKLQAEGVKIPTYALRWVAASYLDQRQPEIATDLYRQVLAAPDADV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
GDRLEDTTALYYSLLESDRAEEARKVAEDQASAQKPRVELKGLPIGNPNDEWMDAQQLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHH
QAGTYGSDLPHGEERLQTLVDQAPGNIGLRLAQADLYLARNWPRRAENQLKETESIAPRD
HCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHCCCCCC
TGLEVAQARTAMDLQEWRQMDALTDDVVARFLDNRQVQRLAREREVHDMSELRVEAYGGK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCC
ANGGSGGDAGAVSGSRDFGIQTTLYSPPIDEDWRVFAGAGYATGDFAEGTGHHRFQRVGL
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHH
ERRTRDMTLEAEVSNHSYGFGDKQGARLAIARDINDHWQYGGSLEYLSAETPLRALNSDI
HHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCCCEEEECCCCCHHHHCCCC
KANGGSGFIRWRANESREWRLAVSPSHFSDGNNRVEALLTGKEGVYSAPNLQVDAGLEVG
CCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEC
TSHNSKSDDVPYFNPKSDFSVMPLLNVNHVLYHRYETAWSQQFQAGAGTYSQRDHGTGGM
CCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
ALLGYGQRYAWNDVFEVGGLFSVINRPYDGDRETDLRLLVDLTFRF
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure 
PRIDGPVLHRGLRPLFRVALCSQLLWPAFAFADTAYDQMVLDARAGHYTPALSVLRQVP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCC
PAQASTGQVSDHLQIASWAGLDAEVVQVYETQGRERALPVQALTATARAYRNLKRWDQAI
CCCCCCCCCCCCEEEHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QVYNKALTLEPDNADLQLGLALTQADSGKPDEGVIRARSLVAAKPDDPSRRLALGYALNR
HHHHHHEEECCCCCCEEEEEEEEECCCCCCCHHHHHHHHHEECCCCCCHHHEEHHHHHHC
AGKPYDALFEYDQAFIRAGNKPEVAREYVVALQKARLPEPALRLARQRPGLIDPVTLRRL
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHH
AGDLAAERVRLAELATRSEKERYVIADRALADYDQLLATWTPDATAHDDVTRWRIDRLGA
HHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LKARARTAEVISEYQKLQAEGVKIPTYALRWVAASYLDQRQPEIATDLYRQVLAAPDADV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
GDRLEDTTALYYSLLESDRAEEARKVAEDQASAQKPRVELKGLPIGNPNDEWMDAQQLAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHH
QAGTYGSDLPHGEERLQTLVDQAPGNIGLRLAQADLYLARNWPRRAENQLKETESIAPRD
HCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHHCCCCCC
TGLEVAQARTAMDLQEWRQMDALTDDVVARFLDNRQVQRLAREREVHDMSELRVEAYGGK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCC
ANGGSGGDAGAVSGSRDFGIQTTLYSPPIDEDWRVFAGAGYATGDFAEGTGHHRFQRVGL
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHH
ERRTRDMTLEAEVSNHSYGFGDKQGARLAIARDINDHWQYGGSLEYLSAETPLRALNSDI
HHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCCHHHCCCCCEEEECCCCCHHHHCCCC
KANGGSGFIRWRANESREWRLAVSPSHFSDGNNRVEALLTGKEGVYSAPNLQVDAGLEVG
CCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEC
TSHNSKSDDVPYFNPKSDFSVMPLLNVNHVLYHRYETAWSQQFQAGAGTYSQRDHGTGGM
CCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
ALLGYGQRYAWNDVFEVGGLFSVINRPYDGDRETDLRLLVDLTFRF
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]