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The map label for this gene is dsbA [H]

Identifier: 77456280

GI number: 77456280

Start: 58135

End: 58776

Strand: Reverse

Name: dsbA [H]

Synonym: Pfl01_0052

Alternate gene names: 77456280

Gene position: 58776-58135 (Counterclockwise)

Preceding gene: 77456281

Following gene: 77456279

Centisome position: 0.91

GC content: 59.81

Gene sequence:

>642_bases
ATGCGTAATCTGATCATCAGCGCCGCCCTCGTCGCTGCCAGCCTGTTCGGCGTCACCGCCCAGGCCGCCGAAGCCCCTGC
TGCCCCTTACGTCGAGCTGAGCAACCCGGTACCGGTCGCCGTGCCTGGCAAGATCGAAGTGGTCGAGCTGTTCTGGTATG
GCTGCCCGCACTGCTACGCGTTCGAGCCGGTGATCAATCCGTGGGTTGAAAAACTGCCGTCCGACGTCAACTTCGTACGC
ATTCCTGCGATGTTCGGTGGCCCGTGGGACGCTCACGGCCAGATGTTCCTGACCCTGGAAGCCATGGGCGTCGAGCACAA
CGTGCACGCTGCCGTTTTCAACGCGATCCAGAAAGAACACAAGAAGCTGACCGACAAGAACGACATGGCCGACTTCCTCG
CCACTCAAGGCGTGGACAAGGACAAGTTCCTGGCGACCTTCGATTCGTTCGCCATCAAAGGGCAGATCGTGAAAGCCCGC
GAACTGGCCAAGAAGTATGAAATCTCCGGTGTTCCAACCATGATCGTCAACGGTAAGTACCGCTTCGACATCGGCTCTGC
CGGCGGTGCCGAACAGGCGCTGAAACTGGCTGACCAACTGGTGGCCAAAGAGCGAGCGACCAACAAGGCAGCCGCCAACT
AA

Upstream 100 bases:

>100_bases
TTTTTTGTGGCCGCTGCCGTTACACTACAGAACTCATGCCCGCCAGGATCTGTCTGAAAACAGGTCGCGCGAGGCGACCA
AAATTGTCCAGGAGTAAAGC

Downstream 100 bases:

>100_bases
GCGCGGCAATCGCCATGCGCCGCTGGGGAACCGAACGCGTCGTTGGCCTGCATGAACCGCAGGTCAACGAGCATCACCTG
GAGTCGACGGGCCTGCCCGC

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRNLIISAALVAASLFGVTAQAAEAPAAPYVELSNPVPVAVPGKIEVVELFWYGCPHCYAFEPVINPWVEKLPSDVNFVR
IPAMFGGPWDAHGQMFLTLEAMGVEHNVHAAVFNAIQKEHKKLTDKNDMADFLATQGVDKDKFLATFDSFAIKGQIVKAR
ELAKKYEISGVPTMIVNGKYRFDIGSAGGAEQALKLADQLVAKERATNKAAAN

Sequences:

>Translated_213_residues
MRNLIISAALVAASLFGVTAQAAEAPAAPYVELSNPVPVAVPGKIEVVELFWYGCPHCYAFEPVINPWVEKLPSDVNFVR
IPAMFGGPWDAHGQMFLTLEAMGVEHNVHAAVFNAIQKEHKKLTDKNDMADFLATQGVDKDKFLATFDSFAIKGQIVKAR
ELAKKYEISGVPTMIVNGKYRFDIGSAGGAEQALKLADQLVAKERATNKAAAN
>Mature_213_residues
MRNLIISAALVAASLFGVTAQAAEAPAAPYVELSNPVPVAVPGKIEVVELFWYGCPHCYAFEPVINPWVEKLPSDVNFVR
IPAMFGGPWDAHGQMFLTLEAMGVEHNVHAAVFNAIQKEHKKLTDKNDMADFLATQGVDKDKFLATFDSFAIKGQIVKAR
ELAKKYEISGVPTMIVNGKYRFDIGSAGGAEQALKLADQLVAKERATNKAAAN

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=179, Percent_Identity=29.608938547486, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23089; Mature: 23089

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLIISAALVAASLFGVTAQAAEAPAAPYVELSNPVPVAVPGKIEVVELFWYGCPHCYA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEEEEECCCCCCEE
FEPVINPWVEKLPSDVNFVRIPAMFGGPWDAHGQMFLTLEAMGVEHNVHAAVFNAIQKEH
ECHHHHHHHHHCCCCCCEEEECCEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
KKLTDKNDMADFLATQGVDKDKFLATFDSFAIKGQIVKARELAKKYEISGVPTMIVNGKY
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCEE
RFDIGSAGGAEQALKLADQLVAKERATNKAAAN
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRNLIISAALVAASLFGVTAQAAEAPAAPYVELSNPVPVAVPGKIEVVELFWYGCPHCYA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCEEEEEEEECCCCCCEE
FEPVINPWVEKLPSDVNFVRIPAMFGGPWDAHGQMFLTLEAMGVEHNVHAAVFNAIQKEH
ECHHHHHHHHHCCCCCCEEEECCEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHH
KKLTDKNDMADFLATQGVDKDKFLATFDSFAIKGQIVKARELAKKYEISGVPTMIVNGKY
HHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEECCEE
RFDIGSAGGAEQALKLADQLVAKERATNKAAAN
EEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]