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The map label for this gene is pksE [H]

Identifier: 75908808

GI number: 75908808

Start: 3214353

End: 3216011

Strand: Direct

Name: pksE [H]

Synonym: Ava_2594

Alternate gene names: 75908808

Gene position: 3214353-3216011 (Clockwise)

Preceding gene: 75908807

Following gene: 75908809

Centisome position: 50.49

GC content: 41.89

Gene sequence:

>1659_bases
GTGACCACTGTTGATGCGTTGATAAATAAATATGATAATGGCTTAGGCTTTTTAGCTTATACCTCATATCAAAATCTAGC
TTGGAAAGGTTCTTTAGACTGCATATCTTTTGACCACACAGCCATAAAAAATAAATTACTGGCTTTAGATAAACCTTGTT
ATATTGTGAAGGTTGCTGGCAAGATTGGTCTCACAAATGACGGTTATTTATCTGCGGCCGAACTTGGGACACCAGGACAA
GTAGAACTTTTAACATCTCTACCATCTATCCGCATTCAACAATTAGGTGACCCCAATTTTCTCTCTTTTTACGGCGTACA
ATCTGCCTATATGACTGGAGCGATGGCTGGTGGTATTGCTTCTGAGGAAATGGTCATTGCTTTAGGTAGAGAAAAAATTC
TCGGTTCTTTTGGTGCAGGTGGTTTACCTCCAGAACGTTTAGAAGTAGCTATTAATCGCATTCAACAAGCGCTACCTCAC
GGCCCCTATGCCTTCAATTTAATTCACAGCCCCAACGATATGGCGATTGAACGCCGGGCAGTGGATTTATACCTCAAATA
TGAGGTAACAACTGTAGAAGCTTCAGCATTTTTAGATTTAACCGCCAACATTGTTTATTATCGGGTGGCGGGGTTAAGTT
TAAATGATGCCAATCAAATTCAAATTAAAAATAAAGTCATTGCCAAAATTTCTCGCCGGGAAGTTGCGAGTAAGTTTATG
CAGCCTGCACCAGCCAGAATTATTAAAGAACTGCTGGAACAAGGTTTAATTACGGAGTTGCAAGCCAAGCTGGCTGCTAA
TGTACCGATGGCGGATGATATTACTGTAGAAGCTGATTCTGGCGGTCATACAGATAATCGTCCCTTAGTTTGCTTGCTGC
CTTCTATTATTGCTTTGCGGGATGAAATTCAAAGACAATATAATTACTCGCAACCTATTAGAGTCGGTGCAGCCGGAGGA
ATTGCCACACCAGAATCAGCATTAGCAGCTTTTATCATGGGTGCTGCTTATGTGGTGACTGGTTCGGTGAATCAAGCTTG
TATTGAATCGGGTGCTTGTGACCATACCAAGCAATTATTAGCGCAAGCGGAAATGGCTGATGTAATTATGGCTCCCGCCG
CCGATATGTTTGAAATGGGAGTGAAATTACAAGTCCTCAAACGGGGGACAATGTTTGCTATGCGGGCGCAAAAATTGTAT
GAACTATACCGCACTTATGACTCGATTGAAGCGATTCCTCCAGCAGAAAGAGAGAAGTTAGAAAAACAAGTTTTTCGCAA
GACAATTGCTGAGGTTTGGGAAGGAACTGCGGCTTATTTATCTCAGAGAAATCCTGAGAAATTGGGTAAGGCAGTTAATA
ATCCTAAATTAAAAATGGCGTTGATTTTCCGTTGGTATTTAGGTTTGTCTTCTCGTTGGTCTAGTGCTGGAGAAAAAGGG
AGAGAAGTAGATTATCAAATTTGGTGCGGCCCGGCAATGGGCGGGTTTAATGACTGGGTGCGCGGTTCTTATTTGGCAGA
AGCAAATAATCGGCGGGTTGTGGATGTGGCTCATCATATTATGACTGGTGCGGCATTCTTATATCGCATTCAAAGTTTGA
AAATTCAAGGAATGCAAATTCCCGATTACTACTGTCAATATCATCCGGTTCGTTACTAG

Upstream 100 bases:

>100_bases
GGTAATAGATAATTGGTCATTCTCTACCCCCCATTACCTATTACCCATTACCCATTACCAATAGGTAGTAATTCATTGCT
AATCGTGCGAGGGAACGACC

Downstream 100 bases:

>100_bases
GAATGGGTAATGGGTGATGGTTAATGGTAATGGGCTGTGGGCTTGTCGAAGAGTCGAAGTAGTTCTCACGCTGAAGTCCG
AATGGTAATGGGTAATAGTT

Product: 2-nitropropane dioxygenase

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MTTVDALINKYDNGLGFLAYTSYQNLAWKGSLDCISFDHTAIKNKLLALDKPCYIVKVAGKIGLTNDGYLSAAELGTPGQ
VELLTSLPSIRIQQLGDPNFLSFYGVQSAYMTGAMAGGIASEEMVIALGREKILGSFGAGGLPPERLEVAINRIQQALPH
GPYAFNLIHSPNDMAIERRAVDLYLKYEVTTVEASAFLDLTANIVYYRVAGLSLNDANQIQIKNKVIAKISRREVASKFM
QPAPARIIKELLEQGLITELQAKLAANVPMADDITVEADSGGHTDNRPLVCLLPSIIALRDEIQRQYNYSQPIRVGAAGG
IATPESALAAFIMGAAYVVTGSVNQACIESGACDHTKQLLAQAEMADVIMAPAADMFEMGVKLQVLKRGTMFAMRAQKLY
ELYRTYDSIEAIPPAEREKLEKQVFRKTIAEVWEGTAAYLSQRNPEKLGKAVNNPKLKMALIFRWYLGLSSRWSSAGEKG
REVDYQIWCGPAMGGFNDWVRGSYLAEANNRRVVDVAHHIMTGAAFLYRIQSLKIQGMQIPDYYCQYHPVRY

Sequences:

>Translated_552_residues
MTTVDALINKYDNGLGFLAYTSYQNLAWKGSLDCISFDHTAIKNKLLALDKPCYIVKVAGKIGLTNDGYLSAAELGTPGQ
VELLTSLPSIRIQQLGDPNFLSFYGVQSAYMTGAMAGGIASEEMVIALGREKILGSFGAGGLPPERLEVAINRIQQALPH
GPYAFNLIHSPNDMAIERRAVDLYLKYEVTTVEASAFLDLTANIVYYRVAGLSLNDANQIQIKNKVIAKISRREVASKFM
QPAPARIIKELLEQGLITELQAKLAANVPMADDITVEADSGGHTDNRPLVCLLPSIIALRDEIQRQYNYSQPIRVGAAGG
IATPESALAAFIMGAAYVVTGSVNQACIESGACDHTKQLLAQAEMADVIMAPAADMFEMGVKLQVLKRGTMFAMRAQKLY
ELYRTYDSIEAIPPAEREKLEKQVFRKTIAEVWEGTAAYLSQRNPEKLGKAVNNPKLKMALIFRWYLGLSSRWSSAGEKG
REVDYQIWCGPAMGGFNDWVRGSYLAEANNRRVVDVAHHIMTGAAFLYRIQSLKIQGMQIPDYYCQYHPVRY
>Mature_551_residues
TTVDALINKYDNGLGFLAYTSYQNLAWKGSLDCISFDHTAIKNKLLALDKPCYIVKVAGKIGLTNDGYLSAAELGTPGQV
ELLTSLPSIRIQQLGDPNFLSFYGVQSAYMTGAMAGGIASEEMVIALGREKILGSFGAGGLPPERLEVAINRIQQALPHG
PYAFNLIHSPNDMAIERRAVDLYLKYEVTTVEASAFLDLTANIVYYRVAGLSLNDANQIQIKNKVIAKISRREVASKFMQ
PAPARIIKELLEQGLITELQAKLAANVPMADDITVEADSGGHTDNRPLVCLLPSIIALRDEIQRQYNYSQPIRVGAAGGI
ATPESALAAFIMGAAYVVTGSVNQACIESGACDHTKQLLAQAEMADVIMAPAADMFEMGVKLQVLKRGTMFAMRAQKLYE
LYRTYDSIEAIPPAEREKLEKQVFRKTIAEVWEGTAAYLSQRNPEKLGKAVNNPKLKMALIFRWYLGLSSRWSSAGEKGR
EVDYQIWCGPAMGGFNDWVRGSYLAEANNRRVVDVAHHIMTGAAFLYRIQSLKIQGMQIPDYYCQYHPVRY

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 60817; Mature: 60686

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVDALINKYDNGLGFLAYTSYQNLAWKGSLDCISFDHTAIKNKLLALDKPCYIVKVAG
CCCHHHHHHHHCCCCEEEEEECCCCEEECCCCCEEECCHHHHHHHHHEECCCEEEEEEEC
KIGLTNDGYLSAAELGTPGQVELLTSLPSIRIQQLGDPNFLSFYGVQSAYMTGAMAGGIA
CCCCCCCCCEEHHCCCCCCCHHHHHCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
SEEMVIALGREKILGSFGAGGLPPERLEVAINRIQQALPHGPYAFNLIHSPNDMAIERRA
CCCEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHEEEEE
VDLYLKYEVTTVEASAFLDLTANIVYYRVAGLSLNDANQIQIKNKVIAKISRREVASKFM
EEEEEEEEEEEEEHHHHHHHHHCEEEEEEECCEECCCCEEEEHHHHHHHHHHHHHHHHHC
QPAPARIIKELLEQGLITELQAKLAANVPMADDITVEADSGGHTDNRPLVCLLPSIIALR
CCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHH
DEIQRQYNYSQPIRVGAAGGIATPESALAAFIMGAAYVVTGSVNQACIESGACDHTKQLL
HHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCHHHHHCCCCHHHHHHH
AQAEMADVIMAPAADMFEMGVKLQVLKRGTMFAMRAQKLYELYRTYDSIEAIPPAEREKL
HHHHHHHHHHCCCHHHHHCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EKQVFRKTIAEVWEGTAAYLSQRNPEKLGKAVNNPKLKMALIFRWYLGLSSRWSSAGEKG
HHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHCCCCC
REVDYQIWCGPAMGGFNDWVRGSYLAEANNRRVVDVAHHIMTGAAFLYRIQSLKIQGMQI
CCCCEEEEECCCCCCCHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCC
PDYYCQYHPVRY
CHHHHCCCCCCC
>Mature Secondary Structure 
TTVDALINKYDNGLGFLAYTSYQNLAWKGSLDCISFDHTAIKNKLLALDKPCYIVKVAG
CCHHHHHHHHCCCCEEEEEECCCCEEECCCCCEEECCHHHHHHHHHEECCCEEEEEEEC
KIGLTNDGYLSAAELGTPGQVELLTSLPSIRIQQLGDPNFLSFYGVQSAYMTGAMAGGIA
CCCCCCCCCEEHHCCCCCCCHHHHHCCCCHHHHHCCCCCEEEECCCHHHHHHHHHHCCCC
SEEMVIALGREKILGSFGAGGLPPERLEVAINRIQQALPHGPYAFNLIHSPNDMAIERRA
CCCEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHEEEEE
VDLYLKYEVTTVEASAFLDLTANIVYYRVAGLSLNDANQIQIKNKVIAKISRREVASKFM
EEEEEEEEEEEEEHHHHHHHHHCEEEEEEECCEECCCCEEEEHHHHHHHHHHHHHHHHHC
QPAPARIIKELLEQGLITELQAKLAANVPMADDITVEADSGGHTDNRPLVCLLPSIIALR
CCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEEHHHHHHHHH
DEIQRQYNYSQPIRVGAAGGIATPESALAAFIMGAAYVVTGSVNQACIESGACDHTKQLL
HHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEECCCCHHHHHCCCCHHHHHHH
AQAEMADVIMAPAADMFEMGVKLQVLKRGTMFAMRAQKLYELYRTYDSIEAIPPAEREKL
HHHHHHHHHHCCCHHHHHCCCEEEEHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EKQVFRKTIAEVWEGTAAYLSQRNPEKLGKAVNNPKLKMALIFRWYLGLSSRWSSAGEKG
HHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCEEHHHHHHHHHCCCHHHHHCCCCC
REVDYQIWCGPAMGGFNDWVRGSYLAEANNRRVVDVAHHIMTGAAFLYRIQSLKIQGMQI
CCCCEEEEECCCCCCCHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCC
PDYYCQYHPVRY
CHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]