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The map label for this gene is ppsB [H]

Identifier: 75908806

GI number: 75908806

Start: 3209345

End: 3210706

Strand: Direct

Name: ppsB [H]

Synonym: Ava_2592

Alternate gene names: 75908806

Gene position: 3209345-3210706 (Clockwise)

Preceding gene: 75908805

Following gene: 75908807

Centisome position: 50.42

GC content: 41.41

Gene sequence:

>1362_bases
GTGGAAAAAATCGCCATCATTGGCTTATCATGCCTGTTTCCTGATGCTAATAATCCTGAGCAATTTTGGCAGAATCTCAC
CGAGCAGAAAGATTCGACATCATCTGTAACTGTTGCCCAAATGGGTGTAGATCCCACAATATTTTACGACCCGGTAAAAG
GCAAGCCGGAAAAATTTTATTTTCTCAAAGGTGGATTTATTCAAAACTTCCAATTTGATGCTAGTGAATACAATCTGCCA
TCAGAATATGTGGAAAGCTTGGATAATACCTTCAAATGGTCGCTATATGCGGCTAAACAAGCAATAAAACATAGTGGTTA
TTGGGGAAATCAAGATGTCCTATCTAAATGTGGAGTCATTTTAGGTACTCTCTCTTTCCCAACTAAGCTTTCTACTCAAC
TGGTTACACCTATTTATCGACAAACTATAGAACCTGCTGTTGCCGAACTTTTACAAAATCAAGATTTTCATCTGGCTGCT
TTACCTACAGCAACTAAACCATCTCTACATAATGCGATGATATCTGGTTTACCAGCCGCATTAGTTGCTCAGGCTTTCTC
TCTATCAGGTACTCATTTCTGTATCGATGCGGCTTGTTCTTCTGCTTTCTACGCTATCAAGTTAGCATCTCATTATCTGT
GGACAGGTAAGGCTGATGTAATGTTAGCTGGTGCTATTAGTTGCTCAGACCCTTTGTTTTTGCGGATGTTGTTTTCTGGG
ATTCAAGGCTATCCAGAAAATGGGATTAGCTCTCCTTTAGATAAATCATCCAGAGGCTTAGTTACATCCGAAGGCATTGG
TATGGTAATGCTAAAACGATATAGCGATGCTGTGAGGGATGGGGATAAAATTCTGGCTACAGTTTGTGGTAATGGTCTTT
CTAATGATGGGAAGGGTAAGCATTTGCTCAGTCCCAACACGAAAGGGCAAGTTACAGCTTTTGAGAGAGCCTATAAAGAA
GCCCAACTTAGCCCCAAAGCTATAGATTATATGGAGTGTCACGCTACCGGCACTTTGTTAGGAGATACGACGGAATTTAA
TTCCATCGAAAGCTTTTTTGGAGAACATCAAGCCGCACCATTAATAGGTTCAAGCAAAGCTAATGTAGGTCACTTGTTAG
TTGCGGCGGGGATGGTGAGTTTAACGAAGGCGATTTACGGCATGAATTATGGTGTGATTCCACCCACGATTAATGTCAAT
GACCCTATAGGTACTGAAAACAATGTCATTTCTCCCCAAAACATTGTTCGGACTCCCACAGCCTGGCCTATCAATACCGG
AACTAAACACGCGGCTTTAAGTGCTTTCGGTTTTGGTGGTACAAACTCCCATCTGATTTTGGAAAAAGCAGCAGATTTGT
AG

Upstream 100 bases:

>100_bases
AGGTCAAAAAAGACTTGTCGAACTCAAGTTATATTAAGTCTGGGGAATAGCGTAAATCCAATAATTCGTAATTCGTTATA
CCCTAACGAGGATATCAGTA

Downstream 100 bases:

>100_bases
CCATAACTTGAGGGCTGAGGGAACACCAAAAAATAGGCGTTGCTGATTAGTTTAGTAGAGTGCGTCAGTATGAATAATTT
CTGAGTATGTTTAGGTTTTA

Product: beta-ketoacyl synthase

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MEKIAIIGLSCLFPDANNPEQFWQNLTEQKDSTSSVTVAQMGVDPTIFYDPVKGKPEKFYFLKGGFIQNFQFDASEYNLP
SEYVESLDNTFKWSLYAAKQAIKHSGYWGNQDVLSKCGVILGTLSFPTKLSTQLVTPIYRQTIEPAVAELLQNQDFHLAA
LPTATKPSLHNAMISGLPAALVAQAFSLSGTHFCIDAACSSAFYAIKLASHYLWTGKADVMLAGAISCSDPLFLRMLFSG
IQGYPENGISSPLDKSSRGLVTSEGIGMVMLKRYSDAVRDGDKILATVCGNGLSNDGKGKHLLSPNTKGQVTAFERAYKE
AQLSPKAIDYMECHATGTLLGDTTEFNSIESFFGEHQAAPLIGSSKANVGHLLVAAGMVSLTKAIYGMNYGVIPPTINVN
DPIGTENNVISPQNIVRTPTAWPINTGTKHAALSAFGFGGTNSHLILEKAADL

Sequences:

>Translated_453_residues
MEKIAIIGLSCLFPDANNPEQFWQNLTEQKDSTSSVTVAQMGVDPTIFYDPVKGKPEKFYFLKGGFIQNFQFDASEYNLP
SEYVESLDNTFKWSLYAAKQAIKHSGYWGNQDVLSKCGVILGTLSFPTKLSTQLVTPIYRQTIEPAVAELLQNQDFHLAA
LPTATKPSLHNAMISGLPAALVAQAFSLSGTHFCIDAACSSAFYAIKLASHYLWTGKADVMLAGAISCSDPLFLRMLFSG
IQGYPENGISSPLDKSSRGLVTSEGIGMVMLKRYSDAVRDGDKILATVCGNGLSNDGKGKHLLSPNTKGQVTAFERAYKE
AQLSPKAIDYMECHATGTLLGDTTEFNSIESFFGEHQAAPLIGSSKANVGHLLVAAGMVSLTKAIYGMNYGVIPPTINVN
DPIGTENNVISPQNIVRTPTAWPINTGTKHAALSAFGFGGTNSHLILEKAADL
>Mature_453_residues
MEKIAIIGLSCLFPDANNPEQFWQNLTEQKDSTSSVTVAQMGVDPTIFYDPVKGKPEKFYFLKGGFIQNFQFDASEYNLP
SEYVESLDNTFKWSLYAAKQAIKHSGYWGNQDVLSKCGVILGTLSFPTKLSTQLVTPIYRQTIEPAVAELLQNQDFHLAA
LPTATKPSLHNAMISGLPAALVAQAFSLSGTHFCIDAACSSAFYAIKLASHYLWTGKADVMLAGAISCSDPLFLRMLFSG
IQGYPENGISSPLDKSSRGLVTSEGIGMVMLKRYSDAVRDGDKILATVCGNGLSNDGKGKHLLSPNTKGQVTAFERAYKE
AQLSPKAIDYMECHATGTLLGDTTEFNSIESFFGEHQAAPLIGSSKANVGHLLVAAGMVSLTKAIYGMNYGVIPPTINVN
DPIGTENNVISPQNIVRTPTAWPINTGTKHAALSAFGFGGTNSHLILEKAADL

Specific function: Polyketide synthase involved in phenolpthiocerol synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Homo sapiens, GI41872631, Length=463, Percent_Identity=25.4859611231102, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI8923559, Length=286, Percent_Identity=31.4685314685315, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI223972627, Length=157, Percent_Identity=33.1210191082803, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1787337, Length=462, Percent_Identity=26.1904761904762, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1788663, Length=291, Percent_Identity=30.2405498281787, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17550940, Length=456, Percent_Identity=26.5350877192982, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71983541, Length=286, Percent_Identity=34.6153846153846, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71983535, Length=261, Percent_Identity=35.632183908046, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI212642053, Length=461, Percent_Identity=28.4164859002169, Blast_Score=131, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6320904, Length=466, Percent_Identity=27.0386266094421, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19920632, Length=451, Percent_Identity=27.7161862527716, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI221330659, Length=451, Percent_Identity=27.7161862527716, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24581345, Length=455, Percent_Identity=26.5934065934066, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24644341, Length=275, Percent_Identity=31.2727272727273, Blast_Score=125, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001227
- InterPro:   IPR009081
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016036
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR020801
- InterPro:   IPR020841
- InterPro:   IPR013968
- InterPro:   IPR020806
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF08659 KR; PF00550 PP-binding [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 48772; Mature: 48772

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIAIIGLSCLFPDANNPEQFWQNLTEQKDSTSSVTVAQMGVDPTIFYDPVKGKPEKFY
CCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCCEEE
FLKGGFIQNFQFDASEYNLPSEYVESLDNTFKWSLYAAKQAIKHSGYWGNQDVLSKCGVI
EEECCEEECEECCCHHCCCCHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCHH
LGTLSFPTKLSTQLVTPIYRQTIEPAVAELLQNQDFHLAALPTATKPSLHNAMISGLPAA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCHHH
LVAQAFSLSGTHFCIDAACSSAFYAIKLASHYLWTGKADVMLAGAISCSDPLFLRMLFSG
HHHHHHHCCCCEEEEHHHHHHHHEEEEEHHHHEECCCCCEEEEEECCCCCHHHHHHHHHH
IQGYPENGISSPLDKSSRGLVTSEGIGMVMLKRYSDAVRDGDKILATVCGNGLSNDGKGK
HCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCCCC
HLLSPNTKGQVTAFERAYKEAQLSPKAIDYMECHATGTLLGDTTEFNSIESFFGEHQAAP
EEECCCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCCEEECCCCCHHHHHHHHCCCCCCC
LIGSSKANVGHLLVAAGMVSLTKAIYGMNYGVIPPTINVNDPIGTENNVISPQNIVRTPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHEECCC
AWPINTGTKHAALSAFGFGGTNSHLILEKAADL
CCCCCCCCCHHHHEEECCCCCCCEEEEEECCCC
>Mature Secondary Structure
MEKIAIIGLSCLFPDANNPEQFWQNLTEQKDSTSSVTVAQMGVDPTIFYDPVKGKPEKFY
CCCEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCEEEECCCCCCCCEEE
FLKGGFIQNFQFDASEYNLPSEYVESLDNTFKWSLYAAKQAIKHSGYWGNQDVLSKCGVI
EEECCEEECEECCCHHCCCCHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCHHHHHHCCHH
LGTLSFPTKLSTQLVTPIYRQTIEPAVAELLQNQDFHLAALPTATKPSLHNAMISGLPAA
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHCCHHH
LVAQAFSLSGTHFCIDAACSSAFYAIKLASHYLWTGKADVMLAGAISCSDPLFLRMLFSG
HHHHHHHCCCCEEEEHHHHHHHHEEEEEHHHHEECCCCCEEEEEECCCCCHHHHHHHHHH
IQGYPENGISSPLDKSSRGLVTSEGIGMVMLKRYSDAVRDGDKILATVCGNGLSNDGKGK
HCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCHHEEHHHHCCCCCCCCCCC
HLLSPNTKGQVTAFERAYKEAQLSPKAIDYMECHATGTLLGDTTEFNSIESFFGEHQAAP
EEECCCCCCCCHHHHHHHHHHCCCCCHHHHEEECCCCEEECCCCCHHHHHHHHCCCCCCC
LIGSSKANVGHLLVAAGMVSLTKAIYGMNYGVIPPTINVNDPIGTENNVISPQNIVRTPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCCCHHHEECCC
AWPINTGTKHAALSAFGFGGTNSHLILEKAADL
CCCCCCCCCHHHHEEECCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]