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The map label for this gene is dusB [H]

Identifier: 75908388

GI number: 75908388

Start: 2683612

End: 2684748

Strand: Direct

Name: dusB [H]

Synonym: Ava_2169

Alternate gene names: 75908388

Gene position: 2683612-2684748 (Clockwise)

Preceding gene: 75908384

Following gene: 75908390

Centisome position: 42.16

GC content: 48.99

Gene sequence:

>1137_bases
ATGTCTATGCCCCAGGTATCGCTGCCCCAATCGCTTCAGAAAGACCAACCCTTCACCGCCCTTGCACCCATGCAGGATGT
GACAAACCTCTGGTTTATGAAGGTCATCGCCCACTACGGTAGTCCTGACTACTTTTTTACTGAGTATTTTCGCGTAAATG
ATACTTCGCGGCTCAATCGCGGTATTCTGGCAGCCATCACCGAAAATGATACAGGTCGCCCTGTTTTTGCCCAAATGATT
GGGGAAAGTATTCCCGACTTGGTGAGAACCGCCAAGGAACTCTGCCACTATGATATCGCTGGAGTTGATTTGAACATGGG
CTGTCCCGCACCGAGAATCTATCGTAAAAATGTCGGGGGTGGATTGCTACTCTCACCTGGGAAAGTGGAGCAGATTTTGG
CAGAACTACGCCAGGCAGTGAGCGATCGCCCCTTAACAGTCAAGATGCGGGTAGGCTTTGAAAATACAGATACCTTTTAC
GAAATTCTCGATATCGTCAATCGCCACAACATTGATTTGCTGAGTCTGCATGGTCGCACAGTCAAAGATATGTACCACGG
GGCAGTGAAATATGATTTGATTGCGGAAGCTGTGAAACGAGTGGATTGTCCAGTACTGGCTAATGGCAATATTCACTCCG
CCACAACTGCCTTAGAAGTGCTGGAGCGAACAGGTGCGGCGGGTGTGATGGTGGGACGCTGGGCTATTGGGAATCCGTGG
CTATTTAATCAGATTCGCCAGGCTTTACGAGGAGAACCAATCACCCCTGTTCCTTTAGTAGAAGTACGCAACTATATTGA
TCGTTTATGGCAAACCCCGACAGCCGCAACTATGCCAGAGCGATCGCGCGTAGGCTACTTAAAAATGTTCCTCAATTATA
TTGCCCTAAGTGTCGATGCTGAAGGTCAGTTCCTGCGACTGATGCGACGGACACAGACCGAAGTGGAAATGTTTAACCTC
TGCGATCGCTTTTTACTTAGTGATCTGACGCAAACTTTAGCCTTAGCACCTTACTCAGGCGTAGGGGAGCAGGGGAGCAG
GGAGCAGGGAGCAGGGGAGAAAGTATTTTCCCTTCCCTGTTCTTCTACCTCTTCGGTCAATAGTCCACAGTCCAAAAACC
TTGATTTTGACCATTGA

Upstream 100 bases:

>100_bases
TGACTTTTATAATTGCAAACTGCCAATTGGCTTGACAAAATATCTCTCGGTGTTTCTATTGTAACATTAGGTATGACAAT
CAAGTTTGTCGATCGCCCGA

Downstream 100 bases:

>100_bases
CTATTGACTATTGACAAAAAAACCAGAAAATTAGTCATACTGCGTAAGTCCTAGTACTATCTCTTTTGACTAAAAAAGGA
GATAAAAGCAGATTTTCTAC

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSMPQVSLPQSLQKDQPFTALAPMQDVTNLWFMKVIAHYGSPDYFFTEYFRVNDTSRLNRGILAAITENDTGRPVFAQMI
GESIPDLVRTAKELCHYDIAGVDLNMGCPAPRIYRKNVGGGLLLSPGKVEQILAELRQAVSDRPLTVKMRVGFENTDTFY
EILDIVNRHNIDLLSLHGRTVKDMYHGAVKYDLIAEAVKRVDCPVLANGNIHSATTALEVLERTGAAGVMVGRWAIGNPW
LFNQIRQALRGEPITPVPLVEVRNYIDRLWQTPTAATMPERSRVGYLKMFLNYIALSVDAEGQFLRLMRRTQTEVEMFNL
CDRFLLSDLTQTLALAPYSGVGEQGSREQGAGEKVFSLPCSSTSSVNSPQSKNLDFDH

Sequences:

>Translated_378_residues
MSMPQVSLPQSLQKDQPFTALAPMQDVTNLWFMKVIAHYGSPDYFFTEYFRVNDTSRLNRGILAAITENDTGRPVFAQMI
GESIPDLVRTAKELCHYDIAGVDLNMGCPAPRIYRKNVGGGLLLSPGKVEQILAELRQAVSDRPLTVKMRVGFENTDTFY
EILDIVNRHNIDLLSLHGRTVKDMYHGAVKYDLIAEAVKRVDCPVLANGNIHSATTALEVLERTGAAGVMVGRWAIGNPW
LFNQIRQALRGEPITPVPLVEVRNYIDRLWQTPTAATMPERSRVGYLKMFLNYIALSVDAEGQFLRLMRRTQTEVEMFNL
CDRFLLSDLTQTLALAPYSGVGEQGSREQGAGEKVFSLPCSSTSSVNSPQSKNLDFDH
>Mature_377_residues
SMPQVSLPQSLQKDQPFTALAPMQDVTNLWFMKVIAHYGSPDYFFTEYFRVNDTSRLNRGILAAITENDTGRPVFAQMIG
ESIPDLVRTAKELCHYDIAGVDLNMGCPAPRIYRKNVGGGLLLSPGKVEQILAELRQAVSDRPLTVKMRVGFENTDTFYE
ILDIVNRHNIDLLSLHGRTVKDMYHGAVKYDLIAEAVKRVDCPVLANGNIHSATTALEVLERTGAAGVMVGRWAIGNPWL
FNQIRQALRGEPITPVPLVEVRNYIDRLWQTPTAATMPERSRVGYLKMFLNYIALSVDAEGQFLRLMRRTQTEVEMFNLC
DRFLLSDLTQTLALAPYSGVGEQGSREQGAGEKVFSLPCSSTSSVNSPQSKNLDFDH

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=29.1845493562232, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239788483, Length=233, Percent_Identity=29.1845493562232, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI8923374, Length=266, Percent_Identity=28.1954887218045, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI31742496, Length=249, Percent_Identity=26.5060240963855, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI40807366, Length=309, Percent_Identity=27.5080906148867, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789660, Length=167, Percent_Identity=40.1197604790419, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788462, Length=298, Percent_Identity=27.1812080536913, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=243, Percent_Identity=27.1604938271605, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17507177, Length=247, Percent_Identity=27.5303643724696, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25144369, Length=200, Percent_Identity=26.5, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323437, Length=242, Percent_Identity=26.0330578512397, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=30.4347826086957, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24580595, Length=234, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI19920448, Length=234, Percent_Identity=25.6410256410256, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 42232; Mature: 42101

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMPQVSLPQSLQKDQPFTALAPMQDVTNLWFMKVIAHYGSPDYFFTEYFRVNDTSRLNR
CCCCCCCCCHHHHCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEHEEEECCHHHHCC
GILAAITENDTGRPVFAQMIGESIPDLVRTAKELCHYDIAGVDLNMGCPAPRIYRKNVGG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHCCCC
GLLLSPGKVEQILAELRQAVSDRPLTVKMRVGFENTDTFYEILDIVNRHNIDLLSLHGRT
CEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCH
VKDMYHGAVKYDLIAEAVKRVDCPVLANGNIHSATTALEVLERTGAAGVMVGRWAIGNPW
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECEECCCCH
LFNQIRQALRGEPITPVPLVEVRNYIDRLWQTPTAATMPERSRVGYLKMFLNYIALSVDA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EGQFLRLMRRTQTEVEMFNLCDRFLLSDLTQTLALAPYSGVGEQGSREQGAGEKVFSLPC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCC
SSTSSVNSPQSKNLDFDH
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SMPQVSLPQSLQKDQPFTALAPMQDVTNLWFMKVIAHYGSPDYFFTEYFRVNDTSRLNR
CCCCCCCCHHHHCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEHEEEECCHHHHCC
GILAAITENDTGRPVFAQMIGESIPDLVRTAKELCHYDIAGVDLNMGCPAPRIYRKNVGG
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHCCCC
GLLLSPGKVEQILAELRQAVSDRPLTVKMRVGFENTDTFYEILDIVNRHNIDLLSLHGRT
CEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCCH
VKDMYHGAVKYDLIAEAVKRVDCPVLANGNIHSATTALEVLERTGAAGVMVGRWAIGNPW
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECEECCCCH
LFNQIRQALRGEPITPVPLVEVRNYIDRLWQTPTAATMPERSRVGYLKMFLNYIALSVDA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
EGQFLRLMRRTQTEVEMFNLCDRFLLSDLTQTLALAPYSGVGEQGSREQGAGEKVFSLPC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCC
SSTSSVNSPQSKNLDFDH
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]