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The map label for this gene is rbsB [H]

Identifier: 75908390

GI number: 75908390

Start: 2686212

End: 2687243

Strand: Direct

Name: rbsB [H]

Synonym: Ava_2171

Alternate gene names: 75908390

Gene position: 2686212-2687243 (Clockwise)

Preceding gene: 75908388

Following gene: 75908391

Centisome position: 42.2

GC content: 46.71

Gene sequence:

>1032_bases
ATGGATGTGAAAAAACAGGCGATGTCTAAAGACAAGCTGCTACGCGTCTACGCAGCAACTTTATTAAGTATTGTCAGTGG
TACTCTTGTCAGCTGCACAAACATTTCTCCCAATGGCCCAACGGCTGCTGACACTGATACCAATACCCAGACCAACAATG
GATCTCCCCATAAATTGCGATCGGTTGGTGTTACCCTGGGGGATTTGAGTAACCCTTTCTTCGTTGTCATGGCCCAGGGA
GCCGAGAAAGAAGCCAAGAAAATCGGTGGTGAGGATGTCAGAGTAACTGTAGTTTCTAGCGGCTATGACCTGAACCAGCA
ATTCAACCAAATTGAGAATTTCGTTGCGGCTAATACTGACCTGATTATCATCAATGCTGCTGACAGTAAAGGAATCAGAC
CAGCCGTTGACCAAGCAAGGCAAGCAGGTAAGGTTGTAATTGCAGTAGATACGGCAATAGAAGCAGACATAGACGCTACC
GTCACCACCAATAATGTGCAAGCGGGAGAAATCAGTTGCCAATATATAGCCGATCGCCTCAAAGGCAAAGGTAATGTAGT
CATAGTCAACGGGCCGCCAGTAACATCGGTAATTCAGCGAGTGGACGGCTGCTTGAAAGTATTAGCCAAATATCCCGATA
TCAAACTACTTTCTAAAGACCAGAATGCAGAAGGTAGCAGAGATGGCGGACTCAGGGTAATGAGTGATTTGTTAGTCACA
TTCCCCAAGATTGATGCTGTCTTTGCCATCAACGATCCTAGCGGTGTGGGAGTAGACCTAGCCGCCAACCAAGCCAAACG
CCAAGACTTTTTCATTGTGGGAGTTGACGGTGCGCCAGAAGCCATAGAAGCGATCGCCTCTGGAGATAGTTTATATGCAG
CAACGGCAACGCAAAACCCCAGAGGAATGACGCAAACAGCCATTCAGGTAGGCAACGACATTTTACATGGCAAAAAACCT
GAATCACCCAATATTTTGATTCCTGCCAAGTTGATTACGAAAGAGAACGTGAGTACATCTACAGGCTGGTAG

Upstream 100 bases:

>100_bases
AGAAAATACCCGCTACAAGGGACGATTAAAGTGGGTTTTAGTTTAGTTAACTTTTCTACACATATTTAAATTTTTCCCAG
TTTTTCTACTTAGGATATAG

Downstream 100 bases:

>100_bases
GGGTGTAGGGGTGTAGGGGTGTAGGGGAGCAACTGACAACTGACAACTGACTATTGACTATTGACCAATGACAACAAATA
TTAAAACAGATTTTTCTCCA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDVKKQAMSKDKLLRVYAATLLSIVSGTLVSCTNISPNGPTAADTDTNTQTNNGSPHKLRSVGVTLGDLSNPFFVVMAQG
AEKEAKKIGGEDVRVTVVSSGYDLNQQFNQIENFVAANTDLIIINAADSKGIRPAVDQARQAGKVVIAVDTAIEADIDAT
VTTNNVQAGEISCQYIADRLKGKGNVVIVNGPPVTSVIQRVDGCLKVLAKYPDIKLLSKDQNAEGSRDGGLRVMSDLLVT
FPKIDAVFAINDPSGVGVDLAANQAKRQDFFIVGVDGAPEAIEAIASGDSLYAATATQNPRGMTQTAIQVGNDILHGKKP
ESPNILIPAKLITKENVSTSTGW

Sequences:

>Translated_343_residues
MDVKKQAMSKDKLLRVYAATLLSIVSGTLVSCTNISPNGPTAADTDTNTQTNNGSPHKLRSVGVTLGDLSNPFFVVMAQG
AEKEAKKIGGEDVRVTVVSSGYDLNQQFNQIENFVAANTDLIIINAADSKGIRPAVDQARQAGKVVIAVDTAIEADIDAT
VTTNNVQAGEISCQYIADRLKGKGNVVIVNGPPVTSVIQRVDGCLKVLAKYPDIKLLSKDQNAEGSRDGGLRVMSDLLVT
FPKIDAVFAINDPSGVGVDLAANQAKRQDFFIVGVDGAPEAIEAIASGDSLYAATATQNPRGMTQTAIQVGNDILHGKKP
ESPNILIPAKLITKENVSTSTGW
>Mature_343_residues
MDVKKQAMSKDKLLRVYAATLLSIVSGTLVSCTNISPNGPTAADTDTNTQTNNGSPHKLRSVGVTLGDLSNPFFVVMAQG
AEKEAKKIGGEDVRVTVVSSGYDLNQQFNQIENFVAANTDLIIINAADSKGIRPAVDQARQAGKVVIAVDTAIEADIDAT
VTTNNVQAGEISCQYIADRLKGKGNVVIVNGPPVTSVIQRVDGCLKVLAKYPDIKLLSKDQNAEGSRDGGLRVMSDLLVT
FPKIDAVFAINDPSGVGVDLAANQAKRQDFFIVGVDGAPEAIEAIASGDSLYAATATQNPRGMTQTAIQVGNDILHGKKP
ESPNILIPAKLITKENVSTSTGW

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=277, Percent_Identity=29.9638989169675, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790526, Length=258, Percent_Identity=31.0077519379845, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789990, Length=265, Percent_Identity=27.9245283018868, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36117; Mature: 36117

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKKQAMSKDKLLRVYAATLLSIVSGTLVSCTNISPNGPTAADTDTNTQTNNGSPHKLR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEH
SVGVTLGDLSNPFFVVMAQGAEKEAKKIGGEDVRVTVVSSGYDLNQQFNQIENFVAANTD
EECEEECCCCCCEEEEEECCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
LIIINAADSKGIRPAVDQARQAGKVVIAVDTAIEADIDATVTTNNVQAGEISCQYIADRL
EEEEECCCCCCCCHHHHHHHHCCCEEEEEECEEECCCCCEEEECCEECCCCHHHHHHHHH
KGKGNVVIVNGPPVTSVIQRVDGCLKVLAKYPDIKLLSKDQNAEGSRDGGLRVMSDLLVT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FPKIDAVFAINDPSGVGVDLAANQAKRQDFFIVGVDGAPEAIEAIASGDSLYAATATQNP
CCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCC
RGMTQTAIQVGNDILHGKKPESPNILIPAKLITKENVSTSTGW
CCHHHHHHHHCCHHHCCCCCCCCCEEEEHHHEECCCCCCCCCC
>Mature Secondary Structure
MDVKKQAMSKDKLLRVYAATLLSIVSGTLVSCTNISPNGPTAADTDTNTQTNNGSPHKLR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEH
SVGVTLGDLSNPFFVVMAQGAEKEAKKIGGEDVRVTVVSSGYDLNQQFNQIENFVAANTD
EECEEECCCCCCEEEEEECCCHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
LIIINAADSKGIRPAVDQARQAGKVVIAVDTAIEADIDATVTTNNVQAGEISCQYIADRL
EEEEECCCCCCCCHHHHHHHHCCCEEEEEECEEECCCCCEEEECCEECCCCHHHHHHHHH
KGKGNVVIVNGPPVTSVIQRVDGCLKVLAKYPDIKLLSKDQNAEGSRDGGLRVMSDLLVT
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
FPKIDAVFAINDPSGVGVDLAANQAKRQDFFIVGVDGAPEAIEAIASGDSLYAATATQNP
CCCCCEEEEECCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCC
RGMTQTAIQVGNDILHGKKPESPNILIPAKLITKENVSTSTGW
CCHHHHHHHHCCHHHCCCCCCCCCEEEEHHHEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]