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The map label for this gene is purA

Identifier: 75908274

GI number: 75908274

Start: 2541482

End: 2542825

Strand: Direct

Name: purA

Synonym: Ava_2053

Alternate gene names: 75908274

Gene position: 2541482-2542825 (Clockwise)

Preceding gene: 75908273

Following gene: 75908275

Centisome position: 39.92

GC content: 48.14

Gene sequence:

>1344_bases
TTGGCTAACGTCATTGTAATAGGCGCTCAATGGGGCGATGAAGGAAAAGGTAAAATAACTGACTTACTCAGCCGCTCCGC
AGATGTGGTAGTACGCTATCAAGGGGGTGTCAATGCTGGACACACAATTGTAGTCAAAGGTCAGACCTTTAAACTGCATT
TGATTCCCTCTGGTATTTTGTACCCCGATACGGAATGTATGATCGGCTGTGGGACGGTCATCGATCCACAAGTTTTGATA
AAAGAACTCGACCAATTAGAAAGTTTAAATATTTCTACTAAAAATCTGCTCATCTCTGAAACAGCCCATGTCACCATGCC
CTATCATCGGCTAATTGACAAGGCATCAGAAGAGCGACGCGGAAGCCATAAAATTGGAACCACTGGTCGAGGGATTGGCC
CTACCTACGCTGATAAATCCGAGCGCACAGGTATCAGGGTATTAGACTTGATGGACCCGGCGGCCCTCCGCGACCAGTTG
GCATGGACAATAAATAATAAGAACCTCATTTTAGAAAAGCTGTATAACCTGCCACCTCTAGATACTGAAGAGGTCGTCAA
AGAATATTTAGGGTATGCAGAACGTTTGAGACCTCACGTTGTTGATACATCTTTAAAGATATATGATGCCATTCAACGGC
GGCGTAACATATTGTTTGAAGGCGCACAAGGTACGCTCTTAGACTTGGATCATGGGACTTATCCCTATGTCACTTCTTCC
AACCCCGTAGCTGGGGGGGCTTGCGTAGGTACAGGTCTAGGCCCGACAATGATAGATCGGGTAATTGGGGTTTCCAAAGC
CTACACAACACGAGTCGGAGAAGGCCCATTCCCTACCGAACTGGATGGGGAGTTAGGGGAATTATTGTGCGATCGCGGGG
CAGAATTTGGCACCACTACCGGACGTAAGCGTCGCTGTGGTTGGTTTGATGCGGTTATCGGTCGCTACGCTGTGCGAATT
AACGGCATGGATTGTATGGCTATCACCAAACTGGATGTCCTTGACGAGTTAGAGGAAATTCAAGTTTGTATAGCCTATGA
AATAGACGGCGATCGCTGCGACCATTTCCCAACTAGCGCTCGTCAGTTTGCCCGGTGTCGTCCTATTTACAAAACCCTCC
CAGGCTGGCAAGTGCCAACAAGCGAATGTCGCACCCTGGAAGACTTACCCCAACAAGCACTGGACTATCTAAAATTCCTC
GCAGAATTGATGGAAGTCCCAATTGCGATCGTTTCTTTAGGCGCAAGCCGCGATCAAACTATCATCGTTGAAGACCCTAT
CCACGGGCCAAAACGTGCCTTGCTGCACCCCGATGGTACTCCCGCCTCTTTGTTGAGTGCATAA

Upstream 100 bases:

>100_bases
CCTAGTGCAGCAGAAAAAATCACTAAAAACTATTGACATTTGTCGCAGACTATGTTGATAATGGTTCTTTGTGGAAACTT
TACCTCAATAAAATAAACAC

Downstream 100 bases:

>100_bases
TCAGTACTAAGTACTAAGTCAAAAGTCCAGAGTCCAGAGTTTTGTGACAACAGACAAATGTACAGACGCGATTAATCGCG
TCTCTAACAACTAACAACCG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MANVIVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVKGQTFKLHLIPSGILYPDTECMIGCGTVIDPQVLI
KELDQLESLNISTKNLLISETAHVTMPYHRLIDKASEERRGSHKIGTTGRGIGPTYADKSERTGIRVLDLMDPAALRDQL
AWTINNKNLILEKLYNLPPLDTEEVVKEYLGYAERLRPHVVDTSLKIYDAIQRRRNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACVGTGLGPTMIDRVIGVSKAYTTRVGEGPFPTELDGELGELLCDRGAEFGTTTGRKRRCGWFDAVIGRYAVRI
NGMDCMAITKLDVLDELEEIQVCIAYEIDGDRCDHFPTSARQFARCRPIYKTLPGWQVPTSECRTLEDLPQQALDYLKFL
AELMEVPIAIVSLGASRDQTIIVEDPIHGPKRALLHPDGTPASLLSA

Sequences:

>Translated_447_residues
MANVIVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVKGQTFKLHLIPSGILYPDTECMIGCGTVIDPQVLI
KELDQLESLNISTKNLLISETAHVTMPYHRLIDKASEERRGSHKIGTTGRGIGPTYADKSERTGIRVLDLMDPAALRDQL
AWTINNKNLILEKLYNLPPLDTEEVVKEYLGYAERLRPHVVDTSLKIYDAIQRRRNILFEGAQGTLLDLDHGTYPYVTSS
NPVAGGACVGTGLGPTMIDRVIGVSKAYTTRVGEGPFPTELDGELGELLCDRGAEFGTTTGRKRRCGWFDAVIGRYAVRI
NGMDCMAITKLDVLDELEEIQVCIAYEIDGDRCDHFPTSARQFARCRPIYKTLPGWQVPTSECRTLEDLPQQALDYLKFL
AELMEVPIAIVSLGASRDQTIIVEDPIHGPKRALLHPDGTPASLLSA
>Mature_446_residues
ANVIVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVKGQTFKLHLIPSGILYPDTECMIGCGTVIDPQVLIK
ELDQLESLNISTKNLLISETAHVTMPYHRLIDKASEERRGSHKIGTTGRGIGPTYADKSERTGIRVLDLMDPAALRDQLA
WTINNKNLILEKLYNLPPLDTEEVVKEYLGYAERLRPHVVDTSLKIYDAIQRRRNILFEGAQGTLLDLDHGTYPYVTSSN
PVAGGACVGTGLGPTMIDRVIGVSKAYTTRVGEGPFPTELDGELGELLCDRGAEFGTTTGRKRRCGWFDAVIGRYAVRIN
GMDCMAITKLDVLDELEEIQVCIAYEIDGDRCDHFPTSARQFARCRPIYKTLPGWQVPTSECRTLEDLPQQALDYLKFLA
ELMEVPIAIVSLGASRDQTIIVEDPIHGPKRALLHPDGTPASLLSA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=425, Percent_Identity=45.4117647058823, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=43.1924882629108, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI40316944, Length=396, Percent_Identity=43.9393939393939, Blast_Score=317, Evalue=1e-86,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=49.6487119437939, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI25146553, Length=427, Percent_Identity=41.92037470726, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=427, Percent_Identity=41.92037470726, Blast_Score=323, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=45.3703703703704, Blast_Score=357, Evalue=3e-99,
Organism=Drosophila melanogaster, GI21356033, Length=423, Percent_Identity=43.0260047281324, Blast_Score=355, Evalue=4e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_ANAVT (Q3MBG1)

Other databases:

- EMBL:   CP000117
- RefSeq:   YP_322570.1
- ProteinModelPortal:   Q3MBG1
- SMR:   Q3MBG1
- STRING:   Q3MBG1
- GeneID:   3680702
- GenomeReviews:   CP000117_GR
- KEGG:   ava:Ava_2053
- NMPDR:   fig|240292.3.peg.3133
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q3MBG1
- ProtClustDB:   PRK01117
- BioCyc:   AVAR240292:AVA_2053-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 49141; Mature: 49010

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVIVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVKGQTFKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
YPDTECMIGCGTVIDPQVLIKELDQLESLNISTKNLLISETAHVTMPYHRLIDKASEERR
CCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEECCCEEEECHHHHHHHHHHHHC
GSHKIGTTGRGIGPTYADKSERTGIRVLDLMDPAALRDQLAWTINNKNLILEKLYNLPPL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCEEEEECCCCHHHHHHHCCCCC
DTEEVVKEYLGYAERLRPHVVDTSLKIYDAIQRRRNILFEGAQGTLLDLDHGTYPYVTSS
CHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCEEECC
NPVAGGACVGTGLGPTMIDRVIGVSKAYTTRVGEGPFPTELDGELGELLCDRGAEFGTTT
CCCCCCCEECCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
GRKRRCGWFDAVIGRYAVRINGMDCMAITKLDVLDELEEIQVCIAYEIDGDRCDHFPTSA
CCCCCCCHHHHHHHCEEEEECCCCEEEEHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHH
RQFARCRPIYKTLPGWQVPTSECRTLEDLPQQALDYLKFLAELMEVPIAIVSLGASRDQT
HHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IIVEDPIHGPKRALLHPDGTPASLLSA
EEEECCCCCCCCEEECCCCCCHHHHCC
>Mature Secondary Structure 
ANVIVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVKGQTFKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEEECCCCC
YPDTECMIGCGTVIDPQVLIKELDQLESLNISTKNLLISETAHVTMPYHRLIDKASEERR
CCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHEEEECCCEEEECHHHHHHHHHHHHC
GSHKIGTTGRGIGPTYADKSERTGIRVLDLMDPAALRDQLAWTINNKNLILEKLYNLPPL
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHCEEEEECCCCHHHHHHHCCCCC
DTEEVVKEYLGYAERLRPHVVDTSLKIYDAIQRRRNILFEGAQGTLLDLDHGTYPYVTSS
CHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCEEECC
NPVAGGACVGTGLGPTMIDRVIGVSKAYTTRVGEGPFPTELDGELGELLCDRGAEFGTTT
CCCCCCCEECCCCCHHHHHHHHCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCC
GRKRRCGWFDAVIGRYAVRINGMDCMAITKLDVLDELEEIQVCIAYEIDGDRCDHFPTSA
CCCCCCCHHHHHHHCEEEEECCCCEEEEHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHH
RQFARCRPIYKTLPGWQVPTSECRTLEDLPQQALDYLKFLAELMEVPIAIVSLGASRDQT
HHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IIVEDPIHGPKRALLHPDGTPASLLSA
EEEECCCCCCCCEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA