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The map label for this gene is rlmB [H]

Identifier: 75676251

GI number: 75676251

Start: 2251111

End: 2251962

Strand: Direct

Name: rlmB [H]

Synonym: Nwi_2062

Alternate gene names: 75676251

Gene position: 2251111-2251962 (Clockwise)

Preceding gene: 75676250

Following gene: 75676256

Centisome position: 66.17

GC content: 66.9

Gene sequence:

>852_bases
ATGAGCGATCGTGATCGAGAGCCGCGATTCCGGCGGGCTTCACGCCCGCGGAGCGACATTCACACCAAGGAGGCCGGAGG
CCGGAACCATCAGCCAAAGCGTGGCTCGGCGCGGAAGGCTCGCGAGACCAGCCCCGATGGTCCGGCGGTGCTCTACGGCT
GGCATACGGTGACAGCCGCGCTGGCCAATCCTCGCCGCCGCATCCGAAAACTGCTCGTCACGGAAAACGCCGCGCGACGG
CTCACGCATGAGAATATCGACACCCCGATCACGCCCGAGATCGTCCGGCCGAGTGCGATCGACCAGCGCCTCGGCCCCGA
CGCGGTGCATCAGGGACTGCTCGCCGAAGCTGATCCCCTGCCCTCGCCCGATATCGAGACGCTGACGCAGGAAGGCATCG
TGCTGGTGCTCGACCAGATTACGGATCCGCACAATGTCGGCGCGATCATGCGATCGGCCGCGGCTTTTTCGGTGAAGGCG
ATCGTCACCACAGCACGCCACAGTCCCGAGGCCACCGGCGTGCTCGCGAAGTCCGCCTCCGGCGCGCTGGAGATCGTGCC
GCTGGTGCTGGTGCAGAATCTGGCGCGAGCGCTGGCCGGGCTGAACGACCGCGGCTTCATGACGGTCGGCCTCGACAGCG
CCGGCAGCGAAAATCTCGCCGCCGTCCCGTTGCGGGAGCCATTGGCTCTGGTGCTGGGCGCCGAAGGCAAAGGTCTACGG
CAATTGACGCGCGACACATGCAGCGCGGTTGCGCGCCTCGACATGCCCGGCCACATCAAGAGCCTGAACGTCTCGAACGC
CGCGGTGCTGGCGCTTTATATCGGCGCCAGCCGTCTCGGCCTCATGCACTGA

Upstream 100 bases:

>100_bases
AAGACGCCTCACAGCTTCGCAGGTTTCTCCGGCCTCCACTATTTCAGATCTCCAAGTTTACGATTCTCGATAGATTCCGC
ACCGTCCGAGACACGCCTAC

Downstream 100 bases:

>100_bases
CGGCGCGACGCCAGATCACATCGGACATCGCAAGCGTTTCATTCATCGGGCGCGCCGGTCAGTCGTGGCGATACTGCACG
GCATGACGGTAGCCATGATG

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSDRDREPRFRRASRPRSDIHTKEAGGRNHQPKRGSARKARETSPDGPAVLYGWHTVTAALANPRRRIRKLLVTENAARR
LTHENIDTPITPEIVRPSAIDQRLGPDAVHQGLLAEADPLPSPDIETLTQEGIVLVLDQITDPHNVGAIMRSAAAFSVKA
IVTTARHSPEATGVLAKSASGALEIVPLVLVQNLARALAGLNDRGFMTVGLDSAGSENLAAVPLREPLALVLGAEGKGLR
QLTRDTCSAVARLDMPGHIKSLNVSNAAVLALYIGASRLGLMH

Sequences:

>Translated_283_residues
MSDRDREPRFRRASRPRSDIHTKEAGGRNHQPKRGSARKARETSPDGPAVLYGWHTVTAALANPRRRIRKLLVTENAARR
LTHENIDTPITPEIVRPSAIDQRLGPDAVHQGLLAEADPLPSPDIETLTQEGIVLVLDQITDPHNVGAIMRSAAAFSVKA
IVTTARHSPEATGVLAKSASGALEIVPLVLVQNLARALAGLNDRGFMTVGLDSAGSENLAAVPLREPLALVLGAEGKGLR
QLTRDTCSAVARLDMPGHIKSLNVSNAAVLALYIGASRLGLMH
>Mature_282_residues
SDRDREPRFRRASRPRSDIHTKEAGGRNHQPKRGSARKARETSPDGPAVLYGWHTVTAALANPRRRIRKLLVTENAARRL
THENIDTPITPEIVRPSAIDQRLGPDAVHQGLLAEADPLPSPDIETLTQEGIVLVLDQITDPHNVGAIMRSAAAFSVKAI
VTTARHSPEATGVLAKSASGALEIVPLVLVQNLARALAGLNDRGFMTVGLDSAGSENLAAVPLREPLALVLGAEGKGLRQ
LTRDTCSAVARLDMPGHIKSLNVSNAAVLALYIGASRLGLMH

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=196, Percent_Identity=33.6734693877551, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1790623, Length=234, Percent_Identity=32.4786324786325, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788935, Length=140, Percent_Identity=31.4285714285714, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=263, Percent_Identity=28.8973384030418, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30268; Mature: 30137

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRDREPRFRRASRPRSDIHTKEAGGRNHQPKRGSARKARETSPDGPAVLYGWHTVTAA
CCCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEHHHHHHH
LANPRRRIRKLLVTENAARRLTHENIDTPITPEIVRPSAIDQRLGPDAVHQGLLAEADPL
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHCCHHHHHHCHHCCCCCC
PSPDIETLTQEGIVLVLDQITDPHNVGAIMRSAAAFSVKAIVTTARHSPEATGVLAKSAS
CCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHCCC
GALEIVPLVLVQNLARALAGLNDRGFMTVGLDSAGSENLAAVPLREPLALVLGAEGKGLR
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCEEEEECCCCCHHH
QLTRDTCSAVARLDMPGHIKSLNVSNAAVLALYIGASRLGLMH
HHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCHHHHCCCC
>Mature Secondary Structure 
SDRDREPRFRRASRPRSDIHTKEAGGRNHQPKRGSARKARETSPDGPAVLYGWHTVTAA
CCCCCCCHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCEEEEEHHHHHHH
LANPRRRIRKLLVTENAARRLTHENIDTPITPEIVRPSAIDQRLGPDAVHQGLLAEADPL
HHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHCCHHHHHHCHHCCCCCC
PSPDIETLTQEGIVLVLDQITDPHNVGAIMRSAAAFSVKAIVTTARHSPEATGVLAKSAS
CCCCHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEHHCCC
GALEIVPLVLVQNLARALAGLNDRGFMTVGLDSAGSENLAAVPLREPLALVLGAEGKGLR
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCEEEEECCCCCHHH
QLTRDTCSAVARLDMPGHIKSLNVSNAAVLALYIGASRLGLMH
HHHHHHHHHHHHCCCCCCEEECCCCCCEEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA