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The map label for this gene is fnrN [H]

Identifier: 75676250

GI number: 75676250

Start: 2249925

End: 2250650

Strand: Direct

Name: fnrN [H]

Synonym: Nwi_2061

Alternate gene names: 75676250

Gene position: 2249925-2250650 (Clockwise)

Preceding gene: 75676249

Following gene: 75676251

Centisome position: 66.13

GC content: 57.02

Gene sequence:

>726_bases
ATGAGCACGTCCCGGAAGGACATCCATAACTCCGATGTGCCGAAGCTCTGTCAGAGTTGTGAGGTACGACACAAAGGCAT
GTGCGGCGCGCTCAACGCCAATGAGTTAGTGGATTTTGCCAAGCATACGCGTGTCGTGAAGGTATCCGCCGGCGATGTTC
TCCTGCAGGAACAGGGATCGATCAGGGCCTACGCGAATGTCATGCGCGGTGTGCTCAAGCTGACCAAGACAATGGCCGAT
GGCCGCCAACAGATCGTCGGACTTCAATTCGCACCCGATTTTGTCGGACGTCTCCATCAAGACGAGAGCCCTGTCCGCGT
CGAAGCCTGTTCAGGCGTTGAGGTCTGCAGCATAACCAGAAACGCGCTGCGGAAGATGCTCGAAAAAAATCCGGCCCTCG
AAACCAAGCTGCTTCACCAGGCCCTGCGCGAGGTCGATCAGGGTCGCGAGTGGATGCTGGCGCTGGGACGAAAGACGGCG
CGTGAAAAGGTGGCCAGCTTTCTGATGATGATGGTTCGGCAGATCGATCCGTTTTCAAGAGGGAACGCCGCCACGGCTTT
CGATCTTCCGCTTACGCGAGCGGAAATGGGCGACTTTCTCGGTTTGACGATTGGAACCGTTTCGCGCGAACTCACGCGAC
TGCGGCGAGTCGGGGTGATCAGGATTTCCTCTCACCGTCACATCGATGTTCTGAATCTAGAAGCGCTTCGGCAATCCGCC
GGTTAA

Upstream 100 bases:

>100_bases
AGACAGGAAGATTTGCGGGATGCAGGCTCCGATCGATGGAACCGCCAGTTCGTCGAAAAAGCGGTTTCCCGGCATACGGT
TAACCTCAAGCTGGAGCGAT

Downstream 100 bases:

>100_bases
ACGGCACAATCCTCGCACGGGCGCTTTCTAGAGCCTTTTCGCTTCTGATGTAATCAGAAGCGAGGCTCTATATTGTTGTT
TTGACGCGTTTTCTTCACGC

Product: Crp family transcriptional regulator

Products: NA

Alternate protein names: ORF-240 [H]

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSTSRKDIHNSDVPKLCQSCEVRHKGMCGALNANELVDFAKHTRVVKVSAGDVLLQEQGSIRAYANVMRGVLKLTKTMAD
GRQQIVGLQFAPDFVGRLHQDESPVRVEACSGVEVCSITRNALRKMLEKNPALETKLLHQALREVDQGREWMLALGRKTA
REKVASFLMMMVRQIDPFSRGNAATAFDLPLTRAEMGDFLGLTIGTVSRELTRLRRVGVIRISSHRHIDVLNLEALRQSA
G

Sequences:

>Translated_241_residues
MSTSRKDIHNSDVPKLCQSCEVRHKGMCGALNANELVDFAKHTRVVKVSAGDVLLQEQGSIRAYANVMRGVLKLTKTMAD
GRQQIVGLQFAPDFVGRLHQDESPVRVEACSGVEVCSITRNALRKMLEKNPALETKLLHQALREVDQGREWMLALGRKTA
REKVASFLMMMVRQIDPFSRGNAATAFDLPLTRAEMGDFLGLTIGTVSRELTRLRRVGVIRISSHRHIDVLNLEALRQSA
G
>Mature_240_residues
STSRKDIHNSDVPKLCQSCEVRHKGMCGALNANELVDFAKHTRVVKVSAGDVLLQEQGSIRAYANVMRGVLKLTKTMADG
RQQIVGLQFAPDFVGRLHQDESPVRVEACSGVEVCSITRNALRKMLEKNPALETKLLHQALREVDQGREWMLALGRKTAR
EKVASFLMMMVRQIDPFSRGNAATAFDLPLTRAEMGDFLGLTIGTVSRELTRLRRVGVIRISSHRHIDVLNLEALRQSAG

Specific function: Promotes the microaerobic and symbiotic induction of fixN, possibly by binding to the FNR consensus binding site upstream of fixN [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=228, Percent_Identity=27.1929824561404, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26803; Mature: 26671

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSRKDIHNSDVPKLCQSCEVRHKGMCGALNANELVDFAKHTRVVKVSAGDVLLQEQGS
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEEEECCCEEEECCCC
IRAYANVMRGVLKLTKTMADGRQQIVGLQFAPDFVGRLHQDESPVRVEACSGVEVCSITR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHCCCCCCCEEEECCCCHHHHHHH
NALRKMLEKNPALETKLLHQALREVDQGREWMLALGRKTAREKVASFLMMMVRQIDPFSR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GNAATAFDLPLTRAEMGDFLGLTIGTVSRELTRLRRVGVIRISSHRHIDVLNLEALRQSA
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEHHHHHHHHHCC
G
C
>Mature Secondary Structure 
STSRKDIHNSDVPKLCQSCEVRHKGMCGALNANELVDFAKHTRVVKVSAGDVLLQEQGS
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCEEEEEECCCEEEECCCC
IRAYANVMRGVLKLTKTMADGRQQIVGLQFAPDFVGRLHQDESPVRVEACSGVEVCSITR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHCCCCCCCEEEECCCCHHHHHHH
NALRKMLEKNPALETKLLHQALREVDQGREWMLALGRKTAREKVASFLMMMVRQIDPFSR
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GNAATAFDLPLTRAEMGDFLGLTIGTVSRELTRLRRVGVIRISSHRHIDVLNLEALRQSA
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCEEHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2175385 [H]